Systematic analysis of O-methyltransferase gene family and identification of potential members involved in the formation of O-methylated flavonoids in Citrus.
Identifieur interne : 000043 ( Main/Exploration ); précédent : 000042; suivant : 000044Systematic analysis of O-methyltransferase gene family and identification of potential members involved in the formation of O-methylated flavonoids in Citrus.
Auteurs : Xiaogang Liu [République populaire de Chine] ; Yan Luo [République populaire de Chine] ; Hongkun Wu [République populaire de Chine] ; Wanpeng Xi [République populaire de Chine] ; Jie Yu [République populaire de Chine] ; Qiuyun Zhang [République populaire de Chine] ; Zhiqin Zhou [République populaire de Chine]Source :
- Gene [ 1879-0038 ] ; 2016.
English descriptors
- KwdEn :
- Citrus sinensis (chemistry), Citrus sinensis (enzymology), Citrus sinensis (genetics), Flavonoids (biosynthesis), Flavonoids (chemistry), Garcinia cambogia (chemistry), Gene Expression Regulation, Developmental, Gene Expression Regulation, Enzymologic, Genome, Plant, Methylation, Multigene Family, Organ Specificity, Phylogeny, Plant Proteins (classification), Plant Proteins (genetics), Plant Proteins (metabolism), Protein O-Methyltransferase (classification), Protein O-Methyltransferase (genetics), Protein O-Methyltransferase (metabolism).
- MESH :
- chemical , biosynthesis : Flavonoids.
- chemistry : Citrus sinensis, Flavonoids, Garcinia cambogia.
- chemical , classification : Plant Proteins, Protein O-Methyltransferase.
- enzymology : Citrus sinensis.
- genetics : Citrus sinensis, Plant Proteins, Protein O-Methyltransferase.
- chemical , metabolism : Plant Proteins, Protein O-Methyltransferase.
- Gene Expression Regulation, Developmental, Gene Expression Regulation, Enzymologic, Genome, Plant, Methylation, Multigene Family, Organ Specificity, Phylogeny.
Abstract
The O-methylation of various secondary metabolites is mainly catalyzed by S-adenosyl-l-methionine (SAM)-dependent O-methyltransferase (OMT) proteins that are encoded by the O-methyltransferase gene family. Citrus fruits are a rich source of O-methylated flavonoids that have a broad spectrum of biological activities, including anti-inflammatory, anticarcinogenic, and antiatherogenic properties. However, little is known about this gene family and its members that are involved in the O-methylation of flavonoids and their regulation in Citrus. In this study, 58 OMT genes were identified from the entire Citrus sinensis genome and compared with those from 3 other representative dicot plants. A comprehensive analysis was performed, including functional/substrate predictions, identification of chromosomal locations, phylogenetic relationships, gene structures, and conserved motifs. Distribution mapping revealed that the 58 OMT genes were unevenly distributed on the 9 citrus chromosomes. Phylogenetic analysis of 164 OMT proteins from C.sinensis, Arabidopsis thaliana, Populus trichocarpa, and Vitis vinifera showed that these proteins were categorized into group I (COMT subfamily) and group II (CCoAOMT subfamily), which were further divided into 10 and 2 subgroups, respectively. Finally, digital gene expression and quantitative real-time polymerase chain reaction analyses revealed that citrus OMT genes had distinct temporal and spatial expression patterns in different tissues and developmental stages. Interestingly, 18 and 11 of the 27 genes predicted to be involved in O-methylation of flavonoids had higher expression in the peel and pulp during fruit development, respectively. The citrus OMT gene family identified in this study might help in the selection of appropriate candidate genes and facilitate functional studies in Citrus.
DOI: 10.1016/j.gene.2015.09.048
PubMed: 26407870
Affiliations:
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Le document en format XML
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<term>Flavonoids (chemistry)</term>
<term>Garcinia cambogia (chemistry)</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Gene Expression Regulation, Enzymologic</term>
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<term>Multigene Family</term>
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<term>Protein O-Methyltransferase (genetics)</term>
<term>Protein O-Methyltransferase (metabolism)</term>
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<term>Flavonoids</term>
<term>Garcinia cambogia</term>
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<term>Gene Expression Regulation, Enzymologic</term>
<term>Genome, Plant</term>
<term>Methylation</term>
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<front><div type="abstract" xml:lang="en">The O-methylation of various secondary metabolites is mainly catalyzed by S-adenosyl-l-methionine (SAM)-dependent O-methyltransferase (OMT) proteins that are encoded by the O-methyltransferase gene family. Citrus fruits are a rich source of O-methylated flavonoids that have a broad spectrum of biological activities, including anti-inflammatory, anticarcinogenic, and antiatherogenic properties. However, little is known about this gene family and its members that are involved in the O-methylation of flavonoids and their regulation in Citrus. In this study, 58 OMT genes were identified from the entire Citrus sinensis genome and compared with those from 3 other representative dicot plants. A comprehensive analysis was performed, including functional/substrate predictions, identification of chromosomal locations, phylogenetic relationships, gene structures, and conserved motifs. Distribution mapping revealed that the 58 OMT genes were unevenly distributed on the 9 citrus chromosomes. Phylogenetic analysis of 164 OMT proteins from C.sinensis, Arabidopsis thaliana, Populus trichocarpa, and Vitis vinifera showed that these proteins were categorized into group I (COMT subfamily) and group II (CCoAOMT subfamily), which were further divided into 10 and 2 subgroups, respectively. Finally, digital gene expression and quantitative real-time polymerase chain reaction analyses revealed that citrus OMT genes had distinct temporal and spatial expression patterns in different tissues and developmental stages. Interestingly, 18 and 11 of the 27 genes predicted to be involved in O-methylation of flavonoids had higher expression in the peel and pulp during fruit development, respectively. The citrus OMT gene family identified in this study might help in the selection of appropriate candidate genes and facilitate functional studies in Citrus.</div>
</front>
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<name sortKey="Yu, Jie" sort="Yu, Jie" uniqKey="Yu J" first="Jie" last="Yu">Jie Yu</name>
<name sortKey="Zhang, Qiuyun" sort="Zhang, Qiuyun" uniqKey="Zhang Q" first="Qiuyun" last="Zhang">Qiuyun Zhang</name>
<name sortKey="Zhou, Zhiqin" sort="Zhou, Zhiqin" uniqKey="Zhou Z" first="Zhiqin" last="Zhou">Zhiqin Zhou</name>
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