Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses
Identifieur interne : 003594 ( Main/Curation ); précédent : 003593; suivant : 003595Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses
Auteurs : R. Johnson [États-Unis] ; J. Cranston [États-Unis] ; E. Chaverra [États-Unis] ; E. Dyer [États-Unis]Source :
- Plant Molecular Biology [ 0167-4412 ] ; 1995-04-01.
English descriptors
- KwdEn :
- Blotting, Northern, Blotting, Southern, Cloning, Molecular, DNA, Complementary (genetics), Edible Grain (embryology), Edible Grain (genetics), Gene Expression, Genes, Plant (genetics), Germination (genetics), Molecular Sequence Data, Polymerase Chain Reaction, RNA, Messenger (analysis), Seeds (genetics), Seeds (growth & development), Sequence Analysis, DNA.
- MESH :
- chemical , analysis : RNA, Messenger.
- chemical , genetics : DNA, Complementary.
- embryology : Edible Grain.
- genetics : Edible Grain, Genes, Plant, Germination, Seeds.
- growth & development : Seeds.
- Blotting, Northern, Blotting, Southern, Cloning, Molecular, Gene Expression, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
Abstract: The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.
Url:
DOI: 10.1007/BF00042043
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000895
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :000895
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000F01
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :003861
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000F43
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000F43
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000F43
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :002242
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :002242
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002242
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :003780
Links to Exploration step
ISTEX:45CE131D98D661242EC8A84CDB197F22CE4E791DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title>
<author><name sortKey="Johnson, R" sort="Johnson, R" uniqKey="Johnson R" first="R." last="Johnson">R. Johnson</name>
</author>
<author><name sortKey="Cranston, J" sort="Cranston, J" uniqKey="Cranston J" first="J." last="Cranston">J. Cranston</name>
</author>
<author><name sortKey="Chaverra, E" sort="Chaverra, E" uniqKey="Chaverra E" first="E." last="Chaverra">E. Chaverra</name>
</author>
<author><name sortKey="Dyer, E" sort="Dyer, E" uniqKey="Dyer E" first="E." last="Dyer">E. Dyer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:45CE131D98D661242EC8A84CDB197F22CE4E791D</idno>
<date when="1995" year="1995">1995</date>
<idno type="doi">10.1007/BF00042043</idno>
<idno type="url">https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000895</idno>
<idno type="wicri:Area/Istex/Curation">000895</idno>
<idno type="wicri:Area/Istex/Checkpoint">000F01</idno>
<idno type="wicri:doubleKey">0167-4412:1995:Johnson R:characterization:of:cdna</idno>
<idno type="wicri:Area/Main/Merge">003861</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:7787176</idno>
<idno type="wicri:Area/PubMed/Corpus">000F43</idno>
<idno type="wicri:Area/PubMed/Curation">000F43</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000F43</idno>
<idno type="wicri:Area/Ncbi/Merge">002242</idno>
<idno type="wicri:Area/Ncbi/Curation">002242</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002242</idno>
<idno type="wicri:doubleKey">0167-4412:1995:Johnson R:characterization:of:cdna</idno>
<idno type="wicri:Area/Main/Merge">003780</idno>
<idno type="wicri:Area/Main/Curation">003594</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title>
<author><name sortKey="Johnson, R" sort="Johnson, R" uniqKey="Johnson R" first="R." last="Johnson">R. Johnson</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cranston, J" sort="Cranston, J" uniqKey="Cranston J" first="J." last="Cranston">J. Cranston</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chaverra, E" sort="Chaverra, E" uniqKey="Chaverra E" first="E." last="Chaverra">E. Chaverra</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dyer, E" sort="Dyer, E" uniqKey="Dyer E" first="E." last="Dyer">E. Dyer</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Plant Molecular Biology</title>
<title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title>
<title level="j" type="abbrev">Plant Mol Biol</title>
<idno type="ISSN">0167-4412</idno>
<idno type="eISSN">1573-5028</idno>
<imprint><publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="1995-04-01">1995-04-01</date>
<biblScope unit="volume">28</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="113">113</biblScope>
<biblScope unit="page" to="122">122</biblScope>
</imprint>
<idno type="ISSN">0167-4412</idno>
</series>
<idno type="istex">45CE131D98D661242EC8A84CDB197F22CE4E791D</idno>
<idno type="DOI">10.1007/BF00042043</idno>
<idno type="ArticleID">BF00042043</idno>
<idno type="ArticleID">Art11</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0167-4412</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Northern</term>
<term>Blotting, Southern</term>
<term>Cloning, Molecular</term>
<term>DNA, Complementary (genetics)</term>
<term>Edible Grain (embryology)</term>
<term>Edible Grain (genetics)</term>
<term>Gene Expression</term>
<term>Genes, Plant (genetics)</term>
<term>Germination (genetics)</term>
<term>Molecular Sequence Data</term>
<term>Polymerase Chain Reaction</term>
<term>RNA, Messenger (analysis)</term>
<term>Seeds (genetics)</term>
<term>Seeds (growth & development)</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Edible Grain</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Edible Grain</term>
<term>Genes, Plant</term>
<term>Germination</term>
<term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Seeds</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term>
<term>Blotting, Southern</term>
<term>Cloning, Molecular</term>
<term>Gene Expression</term>
<term>Molecular Sequence Data</term>
<term>Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.</div>
</front>
</TEI>
<double idat="0167-4412:1995:Johnson R:characterization:of:cdna"><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title>
<author><name sortKey="Johnson, R" sort="Johnson, R" uniqKey="Johnson R" first="R." last="Johnson">R. Johnson</name>
</author>
<author><name sortKey="Cranston, J" sort="Cranston, J" uniqKey="Cranston J" first="J." last="Cranston">J. Cranston</name>
</author>
<author><name sortKey="Chaverra, E" sort="Chaverra, E" uniqKey="Chaverra E" first="E." last="Chaverra">E. Chaverra</name>
</author>
<author><name sortKey="Dyer, E" sort="Dyer, E" uniqKey="Dyer E" first="E." last="Dyer">E. Dyer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:45CE131D98D661242EC8A84CDB197F22CE4E791D</idno>
<date when="1995" year="1995">1995</date>
<idno type="doi">10.1007/BF00042043</idno>
<idno type="url">https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000895</idno>
<idno type="wicri:Area/Istex/Curation">000895</idno>
<idno type="wicri:Area/Istex/Checkpoint">000F01</idno>
<idno type="wicri:doubleKey">0167-4412:1995:Johnson R:characterization:of:cdna</idno>
<idno type="wicri:Area/Main/Merge">003861</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title>
<author><name sortKey="Johnson, R" sort="Johnson, R" uniqKey="Johnson R" first="R." last="Johnson">R. Johnson</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cranston, J" sort="Cranston, J" uniqKey="Cranston J" first="J." last="Cranston">J. Cranston</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chaverra, E" sort="Chaverra, E" uniqKey="Chaverra E" first="E." last="Chaverra">E. Chaverra</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dyer, E" sort="Dyer, E" uniqKey="Dyer E" first="E." last="Dyer">E. Dyer</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Plant Molecular Biology</title>
<title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title>
<title level="j" type="abbrev">Plant Mol Biol</title>
<idno type="ISSN">0167-4412</idno>
<idno type="eISSN">1573-5028</idno>
<imprint><publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="1995-04-01">1995-04-01</date>
<biblScope unit="volume">28</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="113">113</biblScope>
<biblScope unit="page" to="122">122</biblScope>
</imprint>
<idno type="ISSN">0167-4412</idno>
</series>
<idno type="istex">45CE131D98D661242EC8A84CDB197F22CE4E791D</idno>
<idno type="DOI">10.1007/BF00042043</idno>
<idno type="ArticleID">BF00042043</idno>
<idno type="ArticleID">Art11</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0167-4412</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.</div>
</front>
</TEI>
</ISTEX>
<PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses.</title>
<author><name sortKey="Johnson, R R" sort="Johnson, R R" uniqKey="Johnson R" first="R R" last="Johnson">R R Johnson</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, Bozeman 59717-0312, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, Bozeman 59717-0312</wicri:regionArea>
<wicri:noRegion>Bozeman 59717-0312</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cranston, H J" sort="Cranston, H J" uniqKey="Cranston H" first="H J" last="Cranston">H J Cranston</name>
</author>
<author><name sortKey="Chaverra, M E" sort="Chaverra, M E" uniqKey="Chaverra M" first="M E" last="Chaverra">M E Chaverra</name>
</author>
<author><name sortKey="Dyer, W E" sort="Dyer, W E" uniqKey="Dyer W" first="W E" last="Dyer">W E Dyer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1995">1995</date>
<idno type="RBID">pubmed:7787176</idno>
<idno type="pmid">7787176</idno>
<idno type="wicri:Area/PubMed/Corpus">000F43</idno>
<idno type="wicri:Area/PubMed/Curation">000F43</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000F43</idno>
<idno type="wicri:Area/Ncbi/Merge">002242</idno>
<idno type="wicri:Area/Ncbi/Curation">002242</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002242</idno>
<idno type="wicri:doubleKey">0167-4412:1995:Johnson R:characterization:of:cdna</idno>
<idno type="wicri:Area/Main/Merge">003780</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses.</title>
<author><name sortKey="Johnson, R R" sort="Johnson, R R" uniqKey="Johnson R" first="R R" last="Johnson">R R Johnson</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, Bozeman 59717-0312, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant, Soil and Environmental Sciences, Montana State University, Bozeman 59717-0312</wicri:regionArea>
<wicri:noRegion>Bozeman 59717-0312</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cranston, H J" sort="Cranston, H J" uniqKey="Cranston H" first="H J" last="Cranston">H J Cranston</name>
</author>
<author><name sortKey="Chaverra, M E" sort="Chaverra, M E" uniqKey="Chaverra M" first="M E" last="Chaverra">M E Chaverra</name>
</author>
<author><name sortKey="Dyer, W E" sort="Dyer, W E" uniqKey="Dyer W" first="W E" last="Dyer">W E Dyer</name>
</author>
</analytic>
<series><title level="j">Plant molecular biology</title>
<idno type="ISSN">0167-4412</idno>
<imprint><date when="1995" type="published">1995</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Northern</term>
<term>Blotting, Southern</term>
<term>Cloning, Molecular</term>
<term>DNA, Complementary (genetics)</term>
<term>Edible Grain (embryology)</term>
<term>Edible Grain (genetics)</term>
<term>Gene Expression</term>
<term>Genes, Plant (genetics)</term>
<term>Germination (genetics)</term>
<term>Molecular Sequence Data</term>
<term>Polymerase Chain Reaction</term>
<term>RNA, Messenger (analysis)</term>
<term>Seeds (genetics)</term>
<term>Seeds (growth & development)</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Edible Grain</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Edible Grain</term>
<term>Genes, Plant</term>
<term>Germination</term>
<term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Seeds</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term>
<term>Blotting, Southern</term>
<term>Cloning, Molecular</term>
<term>Gene Expression</term>
<term>Molecular Sequence Data</term>
<term>Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of inhibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.</div>
</front>
</TEI>
</PubMed>
</double>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003594 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 003594 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Main |étape= Curation |type= RBID |clé= ISTEX:45CE131D98D661242EC8A84CDB197F22CE4E791D |texte= Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses }}
![]() | This area was generated with Dilib version V0.6.25. | ![]() |