Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses
Identifieur interne : 000895 ( Istex/Corpus ); précédent : 000894; suivant : 000896Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses
Auteurs : R. Johnson ; J. Cranston ; E. Chaverra ; E. DyerSource :
- Plant Molecular Biology [ 0167-4412 ] ; 1995-04-01.
Abstract
Abstract: The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.
Url:
DOI: 10.1007/BF00042043
Links to Exploration step
ISTEX:45CE131D98D661242EC8A84CDB197F22CE4E791DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title><author><name sortKey="Johnson, R" sort="Johnson, R" uniqKey="Johnson R" first="R." last="Johnson">R. Johnson</name><affiliation><mods:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</mods:affiliation></affiliation></author><author><name sortKey="Cranston, J" sort="Cranston, J" uniqKey="Cranston J" first="J." last="Cranston">J. Cranston</name><affiliation><mods:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</mods:affiliation></affiliation></author><author><name sortKey="Chaverra, E" sort="Chaverra, E" uniqKey="Chaverra E" first="E." last="Chaverra">E. Chaverra</name><affiliation><mods:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</mods:affiliation></affiliation></author><author><name sortKey="Dyer, E" sort="Dyer, E" uniqKey="Dyer E" first="E." last="Dyer">E. Dyer</name><affiliation><mods:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:45CE131D98D661242EC8A84CDB197F22CE4E791D</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1007/BF00042043</idno><idno type="url">https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000895</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title><author><name sortKey="Johnson, R" sort="Johnson, R" uniqKey="Johnson R" first="R." last="Johnson">R. Johnson</name><affiliation><mods:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</mods:affiliation></affiliation></author><author><name sortKey="Cranston, J" sort="Cranston, J" uniqKey="Cranston J" first="J." last="Cranston">J. Cranston</name><affiliation><mods:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</mods:affiliation></affiliation></author><author><name sortKey="Chaverra, E" sort="Chaverra, E" uniqKey="Chaverra E" first="E." last="Chaverra">E. Chaverra</name><affiliation><mods:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</mods:affiliation></affiliation></author><author><name sortKey="Dyer, E" sort="Dyer, E" uniqKey="Dyer E" first="E." last="Dyer">E. Dyer</name><affiliation><mods:affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Molecular Biology</title><title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title><title level="j" type="abbrev">Plant Mol Biol</title><idno type="ISSN">0167-4412</idno><idno type="eISSN">1573-5028</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1995-04-01">1995-04-01</date><biblScope unit="volume">28</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="113">113</biblScope><biblScope unit="page" to="122">122</biblScope></imprint><idno type="ISSN">0167-4412</idno></series><idno type="istex">45CE131D98D661242EC8A84CDB197F22CE4E791D</idno><idno type="DOI">10.1007/BF00042043</idno><idno type="ArticleID">BF00042043</idno><idno type="ArticleID">Art11</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-4412</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.</div></front></TEI><istex><corpusName>springer</corpusName><author><json:item><name>Russell R. Johnson</name><affiliations><json:string>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</json:string></affiliations></json:item><json:item><name>Harwood J. Cranston</name><affiliations><json:string>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</json:string></affiliations></json:item><json:item><name>Marta E. Chaverra</name><affiliations><json:string>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</json:string></affiliations></json:item><json:item><name>William E. Dyer</name><affiliations><json:string>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</json:string></affiliations></json:item></author><articleId><json:string>BF00042043</json:string><json:string>Art11</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.</abstract><qualityIndicators><score>6.933</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1533</abstractCharCount><pdfWordCount>4245</pdfWordCount><pdfCharCount>25582</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>224</abstractWordCount></qualityIndicators><title>Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title><genre><json:string>research-article</json:string></genre><host><volume>28</volume><pages><last>122</last><first>113</first></pages><issn><json:string>0167-4412</json:string></issn><issue>1</issue><subject><json:item><value>Biochemistry, general</value></json:item><json:item><value>Plant Sciences</value></json:item><json:item><value>Plant Pathology</value></json:item></subject><journalId><json:string>11103</json:string></journalId><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1573-5028</json:string></eissn><title>Plant Molecular Biology</title><publicationDate>1995</publicationDate><copyrightDate>1995</copyrightDate></host><publicationDate>1995</publicationDate><copyrightDate>1995</copyrightDate><doi><json:string>10.1007/BF00042043</json:string></doi><id>45CE131D98D661242EC8A84CDB197F22CE4E791D</id><score>0.29002142</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><availability><p>Kluwer Academic Publishers, 1995</p></availability><date>1995-10-27</date></publicationStmt><notesStmt><note>Research Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title><author xml:id="author-1"><persName><forename type="first">Russell</forename><surname>Johnson</surname></persName><affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</affiliation></author><author xml:id="author-2"><persName><forename type="first">Harwood</forename><surname>Cranston</surname></persName><affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</affiliation></author><author xml:id="author-3"><persName><forename type="first">Marta</forename><surname>Chaverra</surname></persName><affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</affiliation></author><author xml:id="author-4"><persName><forename type="first">William</forename><surname>Dyer</surname></persName><affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</affiliation></author></analytic><monogr><title level="j">Plant Molecular Biology</title><title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title><title level="j" type="abbrev">Plant Mol Biol</title><idno type="journal-ID">11103</idno><idno type="pISSN">0167-4412</idno><idno type="eISSN">1573-5028</idno><idno type="issue-article-count">19</idno><idno type="volume-issue-count">6</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1995-04-01"></date><biblScope unit="volume">28</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="113">113</biblScope><biblScope unit="page" to="122">122</biblScope></imprint></monogr><idno type="istex">45CE131D98D661242EC8A84CDB197F22CE4E791D</idno><idno type="DOI">10.1007/BF00042043</idno><idno type="ArticleID">BF00042043</idno><idno type="ArticleID">Art11</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1995-10-27</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Biochemistry, general</term></item><item><term>Plant Sciences</term></item><item><term>Plant Pathology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-10-27">Created</change><change when="1995-04-01">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-09-22">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Springer, Publisher found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Kluwer Academic Publishers</PublisherName><PublisherLocation>Dordrecht</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11103</JournalID><JournalPrintISSN>0167-4412</JournalPrintISSN><JournalElectronicISSN>1573-5028</JournalElectronicISSN><JournalTitle>Plant Molecular Biology</JournalTitle><JournalSubTitle>An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</JournalSubTitle><JournalAbbreviatedTitle>Plant Mol Biol</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Biochemistry, general</JournalSubject><JournalSubject Type="Secondary">Plant Sciences</JournalSubject><JournalSubject Type="Secondary">Plant Pathology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>28</VolumeIDStart><VolumeIDEnd>28</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd><IssueArticleCount>19</IssueArticleCount><IssueHistory><CoverDate><Year>1995</Year><Month>4</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>1995</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art11"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00042043</ArticleID><ArticleDOI>10.1007/BF00042043</ArticleDOI><ArticleSequenceNumber>11</ArticleSequenceNumber><ArticleTitle Language="En">Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant <Emphasis Type="Italic">Avena fatua</Emphasis> L. caryopses</ArticleTitle><ArticleCategory>Research Article</ArticleCategory><ArticleFirstPage>113</ArticleFirstPage><ArticleLastPage>122</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>4</Month><Day>27</Day></RegistrationDate><Received><Year>1995</Year><Month>10</Month><Day>27</Day></Received><Accepted><Year>1995</Year><Month>2</Month><Day>23</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>1995</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>11103</JournalID><VolumeIDStart>28</VolumeIDStart><VolumeIDEnd>28</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Russell</GivenName><GivenName>R.</GivenName><FamilyName>Johnson</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Harwood</GivenName><GivenName>J.</GivenName><FamilyName>Cranston</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Marta</GivenName><GivenName>E.</GivenName><FamilyName>Chaverra</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>William</GivenName><GivenName>E.</GivenName><FamilyName>Dyer</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Department of Plant, Soil and Environmental Sciences</OrgDivision><OrgName>Montana State University</OrgName><OrgAddress><Postcode>59717-0312</Postcode><City>Bozeman</City><State>MT</State><Country>USA</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant <Emphasis Type="Italic">Avena fatua</Emphasis> L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA<Subscript>3</Subscript> treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a <Emphasis Type="Italic">Citrus sinensis</Emphasis> glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword>differential display</Keyword><Keyword>dormancy</Keyword><Keyword>RT-PCR</Keyword><Keyword>transcript levels</Keyword><Keyword>wild oats</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses</title></titleInfo><name type="personal"><namePart type="given">Russell</namePart><namePart type="given">R.</namePart><namePart type="family">Johnson</namePart><affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Harwood</namePart><namePart type="given">J.</namePart><namePart type="family">Cranston</namePart><affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marta</namePart><namePart type="given">E.</namePart><namePart type="family">Chaverra</namePart><affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William</namePart><namePart type="given">E.</namePart><namePart type="family">Dyer</namePart><affiliation>Department of Plant, Soil and Environmental Sciences, Montana State University, 59717-0312, Bozeman, MT, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Kluwer Academic Publishers</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateCreated encoding="w3cdtf">1995-10-27</dateCreated><dateIssued encoding="w3cdtf">1995-04-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Abstract: The molecular regulation of seed dormancy was investigated using differential display to visualize and isolate cDNAs representing differentially expressed genes during early imbibition of dormant and nondormant Avena fatua L. embryos. Of about 3000 cDNA bands examined, 5 cDNAs hybridized with mRNAs exhibiting dormancy-associated expression patterns during the first 48 h of imbibition, while many more nondormancy-associated cDNAs were observed. Dormancy-associated clone AFD1 hybridized with a 1.5 kb mRNA barely detectable in dry dormant and nondormant embryos that became more abundant in dormant embryos after 24 h of imbibition. Clone AFD2 hybridized with two mRNAs, a 1.3 kb message constitutively expressed in dormant and nondormant embryos and a 0.9 kb message present at higher levels in dormant embryos after 3 h of imbibition. Nondormancy-associated clones AFN1, AFN2 and AFN3 hybridized with 1.5 kb, 1.7 kb and 1.1 kb mRNAs, respectively, that were more abundant in nondormant embryos during imbibition. Expression patterns of some mRNAs in dormant embryos induced to germinate by GA3 treatment were different than water controls, but were not identical to those observed in nondormant embryos. DNA sequence analysis revealed 76% sequence identity between clone AFN3 and a Citrus sinensis glutathione peroxidase-like cDNA, while significant sequence similarities with known genes were not found for other clones. Southern hybridization analyses showed that all clones represent low (1 to 4) copy number genes.</abstract><note>Research Article</note><relatedItem type="host"><titleInfo><title>Plant Molecular Biology</title><subTitle>An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</subTitle></titleInfo><titleInfo type="abbreviated"><title>Plant Mol Biol</title></titleInfo><genre type="journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1995-04-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Biochemistry, general</topic><topic>Plant Sciences</topic><topic>Plant Pathology</topic></subject><identifier type="ISSN">0167-4412</identifier><identifier type="eISSN">1573-5028</identifier><identifier type="JournalID">11103</identifier><identifier type="IssueArticleCount">19</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1995</date><detail type="volume"><number>28</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>113</start><end>122</end></extent></part><recordInfo><recordOrigin>Kluwer Academic Publishers, 1995</recordOrigin></recordInfo></relatedItem><identifier type="istex">45CE131D98D661242EC8A84CDB197F22CE4E791D</identifier><identifier type="DOI">10.1007/BF00042043</identifier><identifier type="ArticleID">BF00042043</identifier><identifier type="ArticleID">Art11</identifier><accessCondition type="use and reproduction" contentType="copyright">Kluwer Academic Publishers, 1995</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Kluwer Academic Publishers, 1995</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/enrichments/multicat</uri></json:item><istex:refBibTEI uri="https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/enrichments/refBib"><teiHeader></teiHeader><text><front></front><body></body><back><listBibl><biblStruct xml:id="b0"><analytic><title></title><author><persName><forename type="first">B</forename><surname>Alberts</surname></persName></author><author><persName><forename type="first">D</forename><surname>Bray</surname></persName></author><author><persName><forename type="first">J</forename><surname>Lewis</surname></persName></author><author><persName><forename type="first">M</forename><surname>Raft</surname></persName></author><author><persName><forename type="first">K</forename><surname>Roberts</surname></persName></author><author><persName><forename type="first">Jd</forename><surname>Watson</surname></persName></author></analytic><monogr><title level="j">Molecular Biology of the Cell</title><imprint><biblScope unit="page">485</biblScope><date type="published" when="1989"></date></imprint></monogr></biblStruct><biblStruct xml:id="b1"><analytic><title level="a" type="main">Basic local alignment search tool</title><author><persName><forename type="first">Sf</forename><surname>Altshul</surname></persName></author><author><persName><forename type="first">W</forename><surname>Gish</surname></persName></author><author><persName><forename type="first">W</forename><surname>Miller</surname></persName></author><author><persName><forename type="first">Ew</forename><surname>Myers</surname></persName></author><author><persName><forename type="first">Dj</forename><surname>Lipman</surname></persName></author></analytic><monogr><title level="j">J Mol Biol</title><imprint><biblScope unit="volume">215</biblScope><biblScope unit="page" from="403" to="410"></biblScope><date type="published" when="1990"></date></imprint></monogr></biblStruct><biblStruct xml:id="b2"><analytic><title></title><author><persName><forename type="first">Fm</forename><surname>Ausubel</surname></persName></author><author><persName><forename type="first">R</forename><surname>Brent</surname></persName></author><author><persName><forename type="first">Re</forename><surname>Kingston</surname></persName></author><author><persName><forename type="first">Dd</forename><surname>Moore</surname></persName></author><author><persName><forename type="first">Jg</forename><surname>Seidman</surname></persName></author><author><persName><forename type="first">Ja</forename><surname>Smith</surname></persName></author><author><persName><forename type="first">K</forename><surname>Struhl</surname></persName></author></analytic><monogr><title level="j">Current Protocols in Molecular Biology</title><imprint><publisher>John Wiley</publisher><publisher>John Wiley</publisher><date type="published" when="1987"></date></imprint></monogr></biblStruct><biblStruct xml:id="b3"><analytic><title level="a" type="main">Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR)</title><author><persName><forename type="first">D</forename><surname>Bauer</surname></persName></author><author><persName><forename type="first">H</forename><surname>Mtlller</surname></persName></author><author><persName><forename type="first">J</forename><surname>Reich</surname></persName></author><author><persName><forename type="first">H</forename><surname>Riedel</surname></persName></author><author><persName><forename type="first">V</forename><surname>Ahrenkiel</surname></persName></author><author><persName><forename type="first">P</forename><surname>Warthoe</surname></persName></author><author><persName><forename type="first">M</forename><surname>Strauss</surname></persName></author></analytic><monogr><title level="j">Nucl Acids Res</title><imprint><biblScope unit="volume">21</biblScope><biblScope unit="page" from="4272" to="4280"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b4"><analytic><title></title><author><persName><forename type="first">Jd</forename><surname>Bewley</surname></persName></author><author><persName><forename type="first">M</forename><surname>Black</surname></persName></author></analytic><monogr><title level="m">Physiology of Development and Germination</title><meeting><address><addrLine>New York</addrLine></address></meeting><imprint><publisher>Plenum</publisher><date type="published" when="1994"></date></imprint></monogr></biblStruct><biblStruct xml:id="b5"><analytic><title level="a" type="main">Isolation and characterization of a plant cDNA showing homologies to animal glutathione peroxidases</title><author><persName><forename type="first">Mc</forename><surname>Criqui</surname></persName></author><author><persName><forename type="first">E</forename><surname>Jamet</surname></persName></author><author><persName><forename type="first">Y</forename><surname>Parmentier</surname></persName></author><author><persName><forename type="first">J</forename><surname>Marbach</surname></persName></author><author><persName><forename type="first">A</forename><surname>Durr</surname></persName></author><author><persName><forename type="first">J</forename><surname>Fleck</surname></persName></author></analytic><monogr><title level="j">Plant Mol Biol</title><imprint><biblScope unit="volume">18</biblScope><biblScope unit="page" from="623" to="627"></biblScope><date type="published" when="1992"></date></imprint></monogr></biblStruct><biblStruct xml:id="b6"><analytic><title level="a" type="main">Membrane turnover in imbibed and dormant embryos of the wild oat (Avenafatua L.) I. Protein turnover and membrane replacement</title><author><persName><forename type="first">Ac</forename><surname>Cuming</surname></persName></author><author><persName><forename type="first">Dj</forename><surname>Osborne</surname></persName></author></analytic><monogr><title level="j">Planta</title><imprint><biblScope unit="volume">139</biblScope><biblScope unit="page" from="209" to="217"></biblScope><date type="published" when="1978"></date></imprint></monogr></biblStruct><biblStruct xml:id="b7"><analytic><title level="a" type="main">Release of dormancy during after-ripening of Agrostemma githago seeds</title><author><persName><forename type="first">Gj</forename><surname>De Klerk</surname></persName></author></analytic><monogr><title level="j">Physiol Plant</title><imprint><biblScope unit="volume">71</biblScope><biblScope unit="page" from="335" to="340"></biblScope><date type="published" when="1987"></date></imprint></monogr></biblStruct><biblStruct xml:id="b8"><analytic><title level="a" type="main">A delayed-early gene activated by fibroblast growth factor-1 encodes a protein related to aldose reductase</title><author><persName><forename type="first">Pj</forename><surname>Donohoe</surname></persName></author><author><persName><forename type="first">Gf</forename><surname>Alberts</surname></persName></author><author><persName><forename type="first">Bs</forename><surname>Hampton</surname></persName></author><author><persName><forename type="first">Ja</forename><surname>Winkles</surname></persName></author></analytic><monogr><title level="j">J Biol Chem</title><imprint><biblScope unit="volume">269</biblScope><biblScope unit="page" from="8604" to="8609"></biblScope><date type="published" when="1994"></date></imprint></monogr></biblStruct><biblStruct xml:id="b9"><analytic><title level="a" type="main">Dormancy associated embryonic mRNAs and proteins in imbibing Arena fatua L. caryopses</title><author><persName><forename type="first">W</forename><surname>Dyer</surname></persName></author></analytic><monogr><title level="j">Physiol Plant</title><imprint><biblScope unit="volume">88</biblScope><biblScope unit="page" from="201" to="211"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b10"><analytic><title level="a" type="main">A technique for radiolabeling DNA restriction fragments to high specific activity</title><author><persName><forename type="first">Ap</forename><surname>Feinberg</surname></persName></author><author><persName><forename type="first">B</forename><surname>Vogelstein</surname></persName></author></analytic><monogr><title level="j">Analyt Biochem</title><imprint><biblScope unit="volume">132</biblScope><biblScope unit="page" from="6" to="13"></biblScope><date type="published" when="1983"></date></imprint></monogr></biblStruct><biblStruct xml:id="b11"><analytic><title level="a" type="main">Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains</title><author><persName><forename type="first">Gb</forename><surname>Fincher</surname></persName></author></analytic><monogr><title level="j">Annu Rev Plant Physiol Plant Mol Biol</title><imprint><biblScope unit="volume">40</biblScope><biblScope unit="page" from="305" to="346"></biblScope><date type="published" when="1989"></date></imprint></monogr></biblStruct><biblStruct xml:id="b12"><analytic><title level="a" type="main">Identification of a transcriptionally active pseudogene in the chorion locus of the silkmoth Bombyx mori</title><author><persName><forename type="first">M</forename><surname>Fotaki</surname></persName></author><author><persName><forename type="first">K</forename><surname>Iatrou</surname></persName></author></analytic><monogr><title level="j">J Mol Biol</title><imprint><biblScope unit="volume">203</biblScope><biblScope unit="page" from="849" to="860"></biblScope><date type="published" when="1988"></date></imprint></monogr></biblStruct><biblStruct xml:id="b13"><analytic><title level="a" type="main">Cloning and expression of an embryo-specific mRNA up-regulated in hydrated dormant seeds</title><author><persName><forename type="first">Pj</forename><surname>Goldmark</surname></persName></author><author><persName><forename type="first">J</forename><surname>Curry</surname></persName></author><author><persName><forename type="first">Cf</forename><surname>Morris</surname></persName></author><author><persName><forename type="first">Mk</forename><surname>Walker-Simmons</surname></persName></author></analytic><monogr><title level="j">Plant Mol Biol</title><imprint><biblScope unit="volume">19</biblScope><biblScope unit="page" from="433" to="441"></biblScope><date type="published" when="1992"></date></imprint></monogr></biblStruct><biblStruct xml:id="b14"><analytic><title level="a" type="main">The physiology of seed hydration and dehydration and the relation between water stress and the control of germination: a review</title><author><persName><forename type="first">Tw</forename><surname>Hegarty</surname></persName></author></analytic><monogr><title level="j">Plant Cell Environ</title><imprint><biblScope unit="volume">1</biblScope><biblScope unit="page" from="101" to="119"></biblScope><date type="published" when="1978"></date></imprint></monogr></biblStruct><biblStruct xml:id="b15"><analytic><title level="a" type="main">Molecular characterization of salt-stress-associated protein in citrus: protein and cDNA sequence homology to mammalian glutathione peroxidases</title><author><persName><forename type="first">D</forename><surname>Holland</surname></persName></author><author><persName><forename type="first">G</forename><surname>Ben-Hayyim</surname></persName></author><author><persName><forename type="first">Z</forename><surname>Faltin</surname></persName></author><author><persName><forename type="first">L</forename><surname>Camoin</surname></persName></author><author><persName><forename type="first">Ad</forename><surname>Strosberg</surname></persName></author><author><persName><forename type="first">Y</forename><surname>Eshdat</surname></persName></author></analytic><monogr><title level="j">Plant Mol Biol</title><imprint><biblScope unit="volume">21</biblScope><biblScope unit="page" from="923" to="927"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b16"><analytic><title level="a" type="main">Optimization of northern analysis by vacuum-blotting RNA-transfer visualization, and ultraviolet fixation</title><author><persName><forename type="first">Ra</forename><surname>Kroczek</surname></persName></author><author><persName><forename type="first">E</forename><surname>Siebert</surname></persName></author></analytic><monogr><title level="j">Analyt Biochem</title><imprint><biblScope unit="volume">184</biblScope><biblScope unit="page" from="90" to="95"></biblScope><date type="published" when="1990"></date></imprint></monogr></biblStruct><biblStruct xml:id="b17"><analytic><title level="a" type="main">Differential polypeptide patterns in imbibed dormant and after-ripened A venafatua embryos</title><author><persName><forename type="first">B</forename><surname>Li</surname></persName></author><author><persName><forename type="first">Me</forename><surname>Foley</surname></persName></author></analytic><monogr><title level="j">J Exp Bot</title><imprint><biblScope unit="volume">45</biblScope><biblScope unit="page" from="275" to="279"></biblScope><date type="published" when="1994"></date></imprint></monogr></biblStruct><biblStruct xml:id="b18"><analytic><title level="a" type="main">Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization</title><author><persName><forename type="first">P</forename><surname>Liang</surname></persName></author><author><persName><forename type="first">L</forename><surname>Auerboukh</surname></persName></author><author><persName><forename type="first">Ab</forename><surname>Pardee</surname></persName></author></analytic><monogr><title level="j">Nucl Acids Res</title><imprint><biblScope unit="volume">21</biblScope><biblScope unit="page" from="3269" to="3275"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b19"><analytic><title level="a" type="main">Differential display and cloning of messenger RNAs from human breast cancer versus mammary epithelial cells</title><author><persName><forename type="first">P</forename><surname>Liang</surname></persName></author><author><persName><forename type="first">L</forename><surname>Averboukh</surname></persName></author><author><persName><forename type="first">K</forename><surname>Keyomarsi</surname></persName></author><author><persName><forename type="first">R</forename><surname>Sager</surname></persName></author><author><persName><forename type="first">Ab</forename><surname>Pardee</surname></persName></author></analytic><monogr><title level="j">Cancer Res</title><imprint><biblScope unit="volume">52</biblScope><biblScope unit="page" from="6966" to="6968"></biblScope><date type="published" when="1992"></date></imprint></monogr></biblStruct><biblStruct xml:id="b20"><analytic><title level="a" type="main">Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction</title><author><persName><forename type="first">P</forename><surname>Liang</surname></persName></author><author><persName><forename type="first">Ab</forename><surname>Pardee</surname></persName></author></analytic><monogr><title level="j">Science</title><imprint><biblScope unit="volume">257</biblScope><biblScope unit="page" from="967" to="971"></biblScope><date type="published" when="1992"></date></imprint></monogr></biblStruct><biblStruct xml:id="b21"><analytic><title level="a" type="main">Molecular cloning and expression of abscisic acid-responsive genes in embryos of dormant wheat seeds</title><author><persName><forename type="first">Cf</forename><surname>Morris</surname></persName></author><author><persName><forename type="first">Rj</forename><surname>Anderberg</surname></persName></author><author><persName><forename type="first">Pj</forename><surname>Goldmark</surname></persName></author><author><persName><forename type="first">Mk</forename><surname>Walker-Simmons</surname></persName></author></analytic><monogr><title level="j">Plant Physiol</title><imprint><biblScope unit="volume">95</biblScope><biblScope unit="page" from="814" to="821"></biblScope><date type="published" when="1991"></date></imprint></monogr></biblStruct><biblStruct xml:id="b22"><analytic><title level="a" type="main">Improvements to the differential display method for gene analysis</title><author><persName><forename type="first">L</forename><surname>Mou</surname></persName></author><author><persName><forename type="first">H</forename><surname>Miller</surname></persName></author><author><persName><forename type="first">J</forename><surname>Li</surname></persName></author><author><persName><forename type="first">E</forename><surname>Wang</surname></persName></author><author><persName><forename type="first">L</forename><surname>Chalifour</surname></persName></author></analytic><monogr><title level="j">Biochem Biophys Res Commun</title><imprint><biblScope unit="volume">199</biblScope><biblScope unit="page" from="564" to="569"></biblScope><date type="published" when="1994"></date></imprint></monogr></biblStruct><biblStruct xml:id="b23"><analytic><title level="a" type="main">Genetic adaptation for seed dormancy in Avenafatua</title><author><persName><forename type="first">J</forename><surname>Naylor</surname></persName></author><author><persName><forename type="first">Jana</forename><forename type="middle">S</forename></persName></author></analytic><monogr><title level="j">Can J Bot</title><imprint><biblScope unit="volume">54</biblScope><biblScope unit="page" from="306" to="312"></biblScope><date type="published" when="1976"></date></imprint></monogr></biblStruct><biblStruct xml:id="b24"><analytic><title level="a" type="main">Identification by differential display of alpha 6 integrin as a candidate tumor suppressor gene</title><author><persName><forename type="first">R</forename><surname>Sager</surname></persName></author><author><persName><forename type="first">A</forename><surname>Anisowicz</surname></persName></author><author><persName><forename type="first">M</forename><surname>Neveu</surname></persName></author><author><persName><forename type="first">P</forename><surname>Liang</surname></persName></author><author><persName><forename type="first">G</forename><surname>Sotiropoulou</surname></persName></author></analytic><monogr><title level="j">FASEB J</title><imprint><biblScope unit="volume">7</biblScope><biblScope unit="page" from="964" to="970"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b25"><analytic><title level="a" type="main">Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics</title><author><persName><surname>Saghai-Maroofma</surname></persName></author><author><persName><forename type="first">Km</forename><surname>Soliman</surname></persName></author><author><persName><forename type="first">Ra</forename><surname>Jorgensen</surname></persName></author><author><persName><forename type="first">Rw</forename><surname>Allard</surname></persName></author></analytic><monogr><title level="j">Proc Natl Acad Sci</title><imprint><biblScope unit="volume">81</biblScope><biblScope unit="page" from="8014" to="8018"></biblScope><date type="published" when="1984"></date></imprint></monogr></biblStruct><biblStruct xml:id="b26"><analytic><title level="a" type="main">Increased participation of pentose phosphate pathway in response to after ripening and gibberellic acid treatment in caryopses ofA venafatua</title><author><persName><forename type="first">Ja</forename><surname>Simmonds</surname></persName></author><author><persName><forename type="first">Ga</forename><surname>Simpson</surname></persName></author></analytic><monogr><title level="j">Can J Bot</title><imprint><biblScope unit="volume">49</biblScope><biblScope unit="page" from="1833" to="1840"></biblScope><date type="published" when="1971"></date></imprint></monogr></biblStruct><biblStruct xml:id="b27"><monogr><title level="m" type="main">Seed Dormancy in Grasses</title><author><persName><forename type="first">G</forename><surname>Simpson</surname></persName></author><imprint><date type="published" when="1990"></date><publisher>Cambridge University Press</publisher><pubPlace>Cambridge</pubPlace></imprint></monogr></biblStruct><biblStruct xml:id="b28"><analytic><title level="a" type="main">DNA sequence analysis with a modified bacteriophage T7 DNA polymerase</title><author><persName><forename type="first">S</forename><surname>Tabor</surname></persName></author><author><persName><forename type="first">Cc</forename><surname>Richardson</surname></persName></author></analytic><monogr><title level="j">Proc Natl Acad Sci</title><imprint><biblScope unit="volume">84</biblScope><biblScope unit="page" from="767" to="771"></biblScope><date type="published" when="1987"></date></imprint></monogr></biblStruct><biblStruct xml:id="b29"><analytic><title level="a" type="main">Differential cDNA screening strategies to identify novel stage-specific proteins in the developing mammalian brain</title><author><persName><forename type="first">Jb</forename><surname>Watson</surname></persName></author><author><persName><forename type="first">Je</forename><surname>Margulies</surname></persName></author></analytic><monogr><title level="j">Devel Neurosci</title><imprint><biblScope unit="volume">15</biblScope><biblScope unit="page" from="77" to="86"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b30"><analytic><title level="a" type="main">Gene expression screening for specific genes associated with mouse mammary tumor development</title><author><persName><forename type="first">L</forename><surname>Zhang</surname></persName></author><author><persName><forename type="first">D</forename><surname>Medina</surname></persName></author></analytic><monogr><title level="j">Mol Carcinogen</title><imprint><biblScope unit="volume">8</biblScope><biblScope unit="page" from="123" to="126"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct></listBibl></back></text></istex:refBibTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/45CE131D98D661242EC8A84CDB197F22CE4E791D/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000895 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000895 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:45CE131D98D661242EC8A84CDB197F22CE4E791D
|texte= Characterization of cDNA clones for differentially expressed genes in embryos of dormant and nondormant Avena fatua L. caryopses
}}
| This area was generated with Dilib version V0.6.25. |  |