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Cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-dehydration

Identifieur interne : 002C73 ( Main/Curation ); précédent : 002C72; suivant : 002C74

Cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-dehydration

Auteurs : Q. C. Wang [Israël] ; Ö. Batuman [Israël] ; P. Li [Israël] ; M. Bar-Joseph [Israël] ; R. Gafny [Israël]

Source :

Plant cell reports : (Print) [ 0721-7714 ] ; 2002.

RBID : Pascal:02-0373411

Descripteurs français

Pascal (Inist)
- Cryoconservation, Pousse plante, Déshydratation, Agrume, Encapsulation, Citrus sinensis Poncirus trifoliata.
Wicri :
- topic : Déshydratation, Agrume.

English descriptors

KwdEn :
- Citrus fruit, Cryopreservation, Dehydration, Encapsulation, Shoot.

Abstract

In vitro-grown shoot tips excised from preconditioned stock shoots of Troyer' citrange were successfully cryopreserved by encapsulation-dehydration. Optimal survival of cryopreserved shoot tips was achieved when encapsulated shoot tips were dehydrated to 17.1% water content. The sucrose concentration in the preconditioning medium significantly influenced the growth and dry matter percentage of the stock shoots as well as subsequent survival of the cryopreserved shoot tips. Maximal growth of stock shoots was obtained in sucrose concentrations in the range of 0.15 M to 0.29 M, while the dry matter percentage increased as sucrose concentration increased up to 0.44 M. The survival of cryopreserved shoot tips increased from 40% to approximately 80% as the sucrose concentration for stock shoots increased from 0.09 M to 0.22 M or 0.29 M. The benzyladenine concentration in the post-culture medium significantly affected the survival and regrowth of the cryopreserved shoot tips. Survival of the shoot tips was lowest when they were post-cultured on benzyladenine-free medium. However, high benzyladenine concentrations (3-4 μM) induced callus formation. Optimal recovery was obtained in post-culture medium containing 2 μM benzyladenine and 0.05 μM α-naphthalene acetic acid. The extraction of shoot tips from alginate beads greatly improved the regrowth of cryopreserved shoot tips.

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Pascal:02-0373411

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<author><name sortKey="Wang, Q C" sort="Wang, Q C" uniqKey="Wang Q" first="Q. C." last="Wang">Q. C. Wang</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-dehydration</title>
<author><name sortKey="Wang, Q C" sort="Wang, Q C" uniqKey="Wang Q" first="Q. C." last="Wang">Q. C. Wang</name>
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<author><name sortKey="Batuman, O" sort="Batuman, O" uniqKey="Batuman O" first="Ö." last="Batuman">Ö. Batuman</name>
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</author>
<author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P." last="Li">P. Li</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O. Box 6</s1>
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<country>Israël</country>
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</author>
<author><name sortKey="Bar Joseph, M" sort="Bar Joseph, M" uniqKey="Bar Joseph M" first="M." last="Bar-Joseph">M. Bar-Joseph</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O. Box 6</s1>
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<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O. Box 6</s1>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus fruit</term>
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<term>Shoot</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Cryoconservation</term>
<term>Pousse plante</term>
<term>Déshydratation</term>
<term>Agrume</term>
<term>Encapsulation</term>
<term>Citrus sinensis  Poncirus trifoliata</term>
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<front><div type="abstract" xml:lang="en">In vitro-grown shoot tips excised from preconditioned stock shoots of Troyer' citrange were successfully cryopreserved by encapsulation-dehydration. Optimal survival of cryopreserved shoot tips was achieved when encapsulated shoot tips were dehydrated to 17.1% water content. The sucrose concentration in the preconditioning medium significantly influenced the growth and dry matter percentage of the stock shoots as well as subsequent survival of the cryopreserved shoot tips. Maximal growth of stock shoots was obtained in sucrose concentrations in the range of 0.15 M to 0.29 M, while the dry matter percentage increased as sucrose concentration increased up to 0.44 M. The survival of cryopreserved shoot tips increased from 40% to approximately 80% as the sucrose concentration for stock shoots increased from 0.09 M to 0.22 M or 0.29 M. The benzyladenine concentration in the post-culture medium significantly affected the survival and regrowth of the cryopreserved shoot tips. Survival of the shoot tips was lowest when they were post-cultured on benzyladenine-free medium. However, high benzyladenine concentrations (3-4 μM) induced callus formation. Optimal recovery was obtained in post-culture medium containing 2 μM benzyladenine and 0.05 μM α-naphthalene acetic acid. The extraction of shoot tips from alginate beads greatly improved the regrowth of cryopreserved shoot tips.</div>
</front>
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</record>

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Cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-dehydration

Cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-dehydration

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri