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Determination of gamma-aminobutyric acid in food matrices by isotope dilution hydrophilic interaction chromatography coupled to mass spectrometry.

Identifieur interne : 001D88 ( Main/Curation ); précédent : 001D87; suivant : 001D89

Determination of gamma-aminobutyric acid in food matrices by isotope dilution hydrophilic interaction chromatography coupled to mass spectrometry.

Auteurs : Raniero Zazzeroni [Royaume-Uni] ; Andrew Homan ; Emma Thain

Source :

Journal of chromatographic science [ 0021-9665 ] ; 2009.

RBID : pubmed:19772728

English descriptors

KwdEn :
- Beverages (analysis), Chromatography, Liquid (methods), Citrus sinensis (chemistry), Diet, Food Analysis (methods), Humans, Malus (chemistry), Solanum tuberosum (chemistry), Soybeans (chemistry), Spectrometry, Mass, Electrospray Ionization (methods), gamma-Aminobutyric Acid (analysis).
MESH :
- chemical , analysis : gamma-Aminobutyric Acid.
- analysis : Beverages.
- chemistry : Citrus sinensis, Malus, Solanum tuberosum, Soybeans.
- methods : Chromatography, Liquid, Food Analysis, Spectrometry, Mass, Electrospray Ionization.
- Diet, Humans.

Abstract

The estimation of the dietary intake of gamma-aminobutyric acid (GABA) is dependent upon the knowledge of its concentration values in food matrices. To this end, an isotope dilution liquid chromatography-mass spectrometry method has been developed employing the hydrophilic interaction chromatography technique for analyte separation. This approach enabled accurate quantification of GABA in apple, potato, soybeans, and orange juice without the need of a pre- or post-column derivatization reaction. A selective and precise analytical measurement has been obtained with a triple quadrupole mass spectrometer operating in multiple reaction monitoring using the method of standard additions and GABA-d(6) as an internal standard. The concentrations of GABA found in the matrices tested are 7 microg/g of apple, 342 microg/g of potatoes, 211 microg/g of soybeans, and 344 microg/mL of orange juice.

PubMed: 19772728

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pubmed:19772728

Le document en format XML

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<author><name sortKey="Zazzeroni, Raniero" sort="Zazzeroni, Raniero" uniqKey="Zazzeroni R" first="Raniero" last="Zazzeroni">Raniero Zazzeroni</name>
<affiliation wicri:level="1"><nlm:affiliation>Unilever, Safety and Environmental Assurance Centre, Colworth Science Park, Sharnbrook, MK44 1LQ, United Kingdom.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Unilever, Safety and Environmental Assurance Centre, Colworth Science Park, Sharnbrook, MK44 1LQ</wicri:regionArea>
<wicri:noRegion>MK44 1LQ</wicri:noRegion>
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</author>
<author><name sortKey="Homan, Andrew" sort="Homan, Andrew" uniqKey="Homan A" first="Andrew" last="Homan">Andrew Homan</name>
</author>
<author><name sortKey="Thain, Emma" sort="Thain, Emma" uniqKey="Thain E" first="Emma" last="Thain">Emma Thain</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Determination of gamma-aminobutyric acid in food matrices by isotope dilution hydrophilic interaction chromatography coupled to mass spectrometry.</title>
<author><name sortKey="Zazzeroni, Raniero" sort="Zazzeroni, Raniero" uniqKey="Zazzeroni R" first="Raniero" last="Zazzeroni">Raniero Zazzeroni</name>
<affiliation wicri:level="1"><nlm:affiliation>Unilever, Safety and Environmental Assurance Centre, Colworth Science Park, Sharnbrook, MK44 1LQ, United Kingdom.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
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<author><name sortKey="Homan, Andrew" sort="Homan, Andrew" uniqKey="Homan A" first="Andrew" last="Homan">Andrew Homan</name>
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<author><name sortKey="Thain, Emma" sort="Thain, Emma" uniqKey="Thain E" first="Emma" last="Thain">Emma Thain</name>
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<series><title level="j">Journal of chromatographic science</title>
<idno type="ISSN">0021-9665</idno>
<imprint><date when="2009" type="published">2009</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Beverages (analysis)</term>
<term>Chromatography, Liquid (methods)</term>
<term>Citrus sinensis (chemistry)</term>
<term>Diet</term>
<term>Food Analysis (methods)</term>
<term>Humans</term>
<term>Malus (chemistry)</term>
<term>Solanum tuberosum (chemistry)</term>
<term>Soybeans (chemistry)</term>
<term>Spectrometry, Mass, Electrospray Ionization (methods)</term>
<term>gamma-Aminobutyric Acid (analysis)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>gamma-Aminobutyric Acid</term>
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<keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term>
<term>Malus</term>
<term>Solanum tuberosum</term>
<term>Soybeans</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Liquid</term>
<term>Food Analysis</term>
<term>Spectrometry, Mass, Electrospray Ionization</term>
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<keywords scheme="MESH" xml:lang="en"><term>Diet</term>
<term>Humans</term>
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<front><div type="abstract" xml:lang="en">The estimation of the dietary intake of gamma-aminobutyric acid (GABA) is dependent upon the knowledge of its concentration values in food matrices. To this end, an isotope dilution liquid chromatography-mass spectrometry method has been developed employing the hydrophilic interaction chromatography technique for analyte separation. This approach enabled accurate quantification of GABA in apple, potato, soybeans, and orange juice without the need of a pre- or post-column derivatization reaction. A selective and precise analytical measurement has been obtained with a triple quadrupole mass spectrometer operating in multiple reaction monitoring using the method of standard additions and GABA-d(6) as an internal standard. The concentrations of GABA found in the matrices tested are 7 microg/g of apple, 342 microg/g of potatoes, 211 microg/g of soybeans, and 344 microg/mL of orange juice.</div>
</front>
</TEI>
</record>

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Determination of gamma-aminobutyric acid in food matrices by isotope dilution hydrophilic interaction chromatography coupled to mass spectrometry.

Determination of gamma-aminobutyric acid in food matrices by isotope dilution hydrophilic interaction chromatography coupled to mass spectrometry.

Source :

English descriptors

Abstract

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Le document en format XML

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Pour générer des pages wiki