OrangerV1, Istex, Curation, bibRecord, 000B18

Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration

Identifieur interne : 000B18 ( Istex/Curation ); précédent : 000B17; suivant : 000B19

Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration

Auteurs : C. Scottez [France] ; E. Chevreau [France] ; N. Godard [France] ; Y. Arnaud [France] ; M. Duron [France] ; J. Dereuddre [France]

Source :

Cryobiology [ 0011-2240 ] ; 1992.

RBID : ISTEX:C7F3C19F622F8EBEB15762FDF36445AA49922746

Abstract

Cryopreservation of axillary shoot-tips of pear in vitro cultures (Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for 1 week before removal of shoot-tips from beads and subculture onto fresh medium. Shoot recovery from cryopreserved shoot-tips was greatly improved by 8–12 weeks of cold acclimation at 0 °C of donor in vitro cultures. The best results (80% shoot recovery) were obtained using 0.75 M sucrose for preculture and 4-h dehydration (giving 20% residual water). The resistance of encapsulated and dehydrated shoot-tips to liquid nitrogen did not depend on cooling rate. Apical shoot-tips about 3 mm in length with several axillary buds were also cryopreserved successfully (47% shoot recovery).

Url:

https://api.istex.fr/document/C7F3C19F622F8EBEB15762FDF36445AA49922746/fulltext/pdf

DOI: 10.1016/0011-2240(92)90073-B

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000B18

Links to Exploration step

ISTEX:C7F3C19F622F8EBEB15762FDF36445AA49922746

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration</title>
<author><name sortKey="Scottez, C" sort="Scottez, C" uniqKey="Scottez C" first="C." last="Scottez">C. Scottez</name>
<affiliation wicri:level="1"><mods:affiliation>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chevreau, E" sort="Chevreau, E" uniqKey="Chevreau E" first="E." last="Chevreau">E. Chevreau</name>
<affiliation wicri:level="1"><mods:affiliation>CTIFL, Centre de Balandran, F 30127 Bellegarde, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>CTIFL, Centre de Balandran, F 30127 Bellegarde</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Godard, N" sort="Godard, N" uniqKey="Godard N" first="N." last="Godard">N. Godard</name>
<affiliation wicri:level="1"><mods:affiliation>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Arnaud, Y" sort="Arnaud, Y" uniqKey="Arnaud Y" first="Y." last="Arnaud">Y. Arnaud</name>
<affiliation wicri:level="1"><mods:affiliation>Laboratoire de Physiologie du Développement des Plantes, Université Paris VI, F 75005 Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Physiologie du Développement des Plantes, Université Paris VI, F 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Duron, M" sort="Duron, M" uniqKey="Duron M" first="M." last="Duron">M. Duron</name>
<affiliation wicri:level="1"><mods:affiliation>INRA, Station d'Amélioration des Espèces Fruitières et Ornementales, F 49070 Beaucouzé, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INRA, Station d'Amélioration des Espèces Fruitières et Ornementales, F 49070 Beaucouzé</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dereuddre, J" sort="Dereuddre, J" uniqKey="Dereuddre J" first="J." last="Dereuddre">J. Dereuddre</name>
<affiliation wicri:level="1"><mods:affiliation>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:C7F3C19F622F8EBEB15762FDF36445AA49922746</idno>
<date when="1992" year="1992">1992</date>
<idno type="doi">10.1016/0011-2240(92)90073-B</idno>
<idno type="url">https://api.istex.fr/document/C7F3C19F622F8EBEB15762FDF36445AA49922746/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000B18</idno>
<idno type="wicri:Area/Istex/Curation">000B18</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration</title>
<author><name sortKey="Scottez, C" sort="Scottez, C" uniqKey="Scottez C" first="C." last="Scottez">C. Scottez</name>
<affiliation wicri:level="1"><mods:affiliation>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chevreau, E" sort="Chevreau, E" uniqKey="Chevreau E" first="E." last="Chevreau">E. Chevreau</name>
<affiliation wicri:level="1"><mods:affiliation>CTIFL, Centre de Balandran, F 30127 Bellegarde, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>CTIFL, Centre de Balandran, F 30127 Bellegarde</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Godard, N" sort="Godard, N" uniqKey="Godard N" first="N." last="Godard">N. Godard</name>
<affiliation wicri:level="1"><mods:affiliation>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Arnaud, Y" sort="Arnaud, Y" uniqKey="Arnaud Y" first="Y." last="Arnaud">Y. Arnaud</name>
<affiliation wicri:level="1"><mods:affiliation>Laboratoire de Physiologie du Développement des Plantes, Université Paris VI, F 75005 Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Physiologie du Développement des Plantes, Université Paris VI, F 75005 Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Duron, M" sort="Duron, M" uniqKey="Duron M" first="M." last="Duron">M. Duron</name>
<affiliation wicri:level="1"><mods:affiliation>INRA, Station d'Amélioration des Espèces Fruitières et Ornementales, F 49070 Beaucouzé, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INRA, Station d'Amélioration des Espèces Fruitières et Ornementales, F 49070 Beaucouzé</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dereuddre, J" sort="Dereuddre, J" uniqKey="Dereuddre J" first="J." last="Dereuddre">J. Dereuddre</name>
<affiliation wicri:level="1"><mods:affiliation>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cryobiology</title>
<title level="j" type="abbrev">YCRYO</title>
<idno type="ISSN">0011-2240</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1992">1992</date>
<biblScope unit="volume">29</biblScope>
<biblScope unit="issue">6</biblScope>
<biblScope unit="page" from="691">691</biblScope>
<biblScope unit="page" to="700">700</biblScope>
</imprint>
<idno type="ISSN">0011-2240</idno>
</series>
<idno type="istex">C7F3C19F622F8EBEB15762FDF36445AA49922746</idno>
<idno type="DOI">10.1016/0011-2240(92)90073-B</idno>
<idno type="PII">0011-2240(92)90073-B</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0011-2240</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Cryopreservation of axillary shoot-tips of pear in vitro cultures (Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for 1 week before removal of shoot-tips from beads and subculture onto fresh medium. Shoot recovery from cryopreserved shoot-tips was greatly improved by 8–12 weeks of cold acclimation at 0 °C of donor in vitro cultures. The best results (80% shoot recovery) were obtained using 0.75 M sucrose for preculture and 4-h dehydration (giving 20% residual water). The resistance of encapsulated and dehydrated shoot-tips to liquid nitrogen did not depend on cooling rate. Apical shoot-tips about 3 mm in length with several axillary buds were also cryopreserved successfully (47% shoot recovery).</div>
</front>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Istex/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B18 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000B18 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Istex
   |étape=   Curation
   |type=    RBID
   |clé=     ISTEX:C7F3C19F622F8EBEB15762FDF36445AA49922746
   |texte=   Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration

Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri