Cold-induced changes in ribulose 1,5-bisphosphate carboxylase-oxygenase and phosphoenolpyruvate carboxylase in citrus
Identifieur interne : 000344 ( Istex/Corpus ); précédent : 000343; suivant : 000345Cold-induced changes in ribulose 1,5-bisphosphate carboxylase-oxygenase and phosphoenolpyruvate carboxylase in citrus
Auteurs : Joseph C. V. Vu ; Sanjay K. Gupta ; George Yelenosky ; Maurice S. B. KuSource :
- Environmental and Experimental Botany [ 0098-8472 ] ; 1995.
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- KwdEn :
Abstract
Abstract: The effects of cold acclimation on ribulose 1,5-bisphosphate carboxylase-oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPCase) were examined in leaves of moderately cold-hardy ‘Valencia’ orange (Citrus sinensis L. Osbeck) and in two cold-hardy genotypes, ‘Trifoliata’ orange (Poncirus trifoliata L. Raf.) and ‘FF-6-7-2’ citrus hybrid (Citrus ichangensis Swing. X Fortunella margarita Lour. Swing.). Soluble protein extracted from leaves and analyzed by electrophoresis showed significant differences in composition among the three citrus genotypes. A 12 kD polypeptide with unknown function was present in large quantity in ‘Valencia’, but not in the other two genotypes. Probing with antibodies specific for Rubisco and PEPCase indicated that ‘Trifoliata’ had two polypeptides of Rubisco small subunit (13 and 14 kD) whereas ‘Valencia’ and ‘FF-6-7-2’ had only one (14 kD). Cold acclimation differentially altered the expression of the two carboxylases. In all three genotypes, cold acclimation resulted in increased amount of Rubisco large subunit, but decreased amount of the small subunit. However, the amount of PEPCase was substantially enhanced by cold acclimation in the cold-hardy genotypes (‘FF-6-7-2’ and ‘Trifoliata’), but was largely reduced by the cold treatment in the moderately cold-hardy genotype (‘Valencia’). The enhanced expression of PEPCase during cold acclimation may be important for the induction of freezing tolerance in citrus.
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DOI: 10.1016/0098-8472(94)00044-6
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cold-induced changes in ribulose 1,5-bisphosphate carboxylase-oxygenase and phosphoenolpyruvate carboxylase in citrus</title><author><name sortKey="Vu, Joseph C V" sort="Vu, Joseph C V" uniqKey="Vu J" first="Joseph C. V." last="Vu">Joseph C. V. Vu</name><affiliation><mods:affiliation>U.S. Department of Agriculture, Agricultural Research Service, Agronomy Physiology Laboratory, Building 164, University of Florida, Gainesville, FL 32611-0840, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Gupta, Sanjay K" sort="Gupta, Sanjay K" uniqKey="Gupta S" first="Sanjay K." last="Gupta">Sanjay K. Gupta</name><affiliation><mods:affiliation>Botany Department, Washington State University, Pullman, WA 99164-4238, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Yelenosky, George" sort="Yelenosky, George" uniqKey="Yelenosky G" first="George" last="Yelenosky">George Yelenosky</name><affiliation><mods:affiliation>U.S. Horticultural Research Laboratory, 2120 Camden Road, Orlando, FL 32803-1419, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Ku, Maurice S B" sort="Ku, Maurice S B" uniqKey="Ku M" first="Maurice S. B." last="Ku">Maurice S. B. 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Vu</name><affiliation><mods:affiliation>U.S. Department of Agriculture, Agricultural Research Service, Agronomy Physiology Laboratory, Building 164, University of Florida, Gainesville, FL 32611-0840, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Gupta, Sanjay K" sort="Gupta, Sanjay K" uniqKey="Gupta S" first="Sanjay K." last="Gupta">Sanjay K. Gupta</name><affiliation><mods:affiliation>Botany Department, Washington State University, Pullman, WA 99164-4238, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Yelenosky, George" sort="Yelenosky, George" uniqKey="Yelenosky G" first="George" last="Yelenosky">George Yelenosky</name><affiliation><mods:affiliation>U.S. Horticultural Research Laboratory, 2120 Camden Road, Orlando, FL 32803-1419, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Ku, Maurice S B" sort="Ku, Maurice S B" uniqKey="Ku M" first="Maurice S. B." last="Ku">Maurice S. B. Ku</name><affiliation><mods:affiliation>Botany Department, Washington State University, Pullman, WA 99164-4238, U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Environmental and Experimental Botany</title><title level="j" type="abbrev">EEB</title><idno type="ISSN">0098-8472</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">35</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="25">25</biblScope><biblScope unit="page" to="31">31</biblScope></imprint><idno type="ISSN">0098-8472</idno></series><idno type="istex">84D93BE3493DF9413C2B1EFE345D21B8D24E6210</idno><idno type="DOI">10.1016/0098-8472(94)00044-6</idno><idno type="PII">0098-8472(94)00044-6</idno><idno type="ArticleID">94000446</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0098-8472</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus</term><term>LSU</term><term>PEPCase</term><term>PPFD</term><term>Rubisco</term><term>SDS-PAGE</term><term>SSU</term><term>cold acclimation</term><term>protein expression</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The effects of cold acclimation on ribulose 1,5-bisphosphate carboxylase-oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPCase) were examined in leaves of moderately cold-hardy ‘Valencia’ orange (Citrus sinensis L. Osbeck) and in two cold-hardy genotypes, ‘Trifoliata’ orange (Poncirus trifoliata L. Raf.) and ‘FF-6-7-2’ citrus hybrid (Citrus ichangensis Swing. X Fortunella margarita Lour. Swing.). Soluble protein extracted from leaves and analyzed by electrophoresis showed significant differences in composition among the three citrus genotypes. A 12 kD polypeptide with unknown function was present in large quantity in ‘Valencia’, but not in the other two genotypes. Probing with antibodies specific for Rubisco and PEPCase indicated that ‘Trifoliata’ had two polypeptides of Rubisco small subunit (13 and 14 kD) whereas ‘Valencia’ and ‘FF-6-7-2’ had only one (14 kD). Cold acclimation differentially altered the expression of the two carboxylases. In all three genotypes, cold acclimation resulted in increased amount of Rubisco large subunit, but decreased amount of the small subunit. However, the amount of PEPCase was substantially enhanced by cold acclimation in the cold-hardy genotypes (‘FF-6-7-2’ and ‘Trifoliata’), but was largely reduced by the cold treatment in the moderately cold-hardy genotype (‘Valencia’). The enhanced expression of PEPCase during cold acclimation may be important for the induction of freezing tolerance in citrus.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Joseph C.V. Vu</name><affiliations><json:string>U.S. Department of Agriculture, Agricultural Research Service, Agronomy Physiology Laboratory, Building 164, University of Florida, Gainesville, FL 32611-0840, U.S.A.</json:string></affiliations></json:item><json:item><name>Sanjay K. Gupta</name><affiliations><json:string>Botany Department, Washington State University, Pullman, WA 99164-4238, U.S.A.</json:string></affiliations></json:item><json:item><name>George Yelenosky</name><affiliations><json:string>U.S. Horticultural Research Laboratory, 2120 Camden Road, Orlando, FL 32803-1419, U.S.A.</json:string></affiliations></json:item><json:item><name>Maurice S.B. Ku</name><affiliations><json:string>Botany Department, Washington State University, Pullman, WA 99164-4238, U.S.A.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Citrus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cold acclimation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>protein expression</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LSU : large subunit</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PEPCase : phosphoenolpyruvate carboxylase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PPFD : photosynthetic photon flux density</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Rubisco : ribulose 1,5-bisphosphate carboxylase-oxygenase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SDS-PAGE : sodium dodecyl sulfate-polyacrylamide gel electrophoresis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SSU : small subunit</value></json:item></subject><articleId><json:string>94000446</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>The effects of cold acclimation on ribulose 1,5-bisphosphate carboxylase-oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPCase) were examined in leaves of moderately cold-hardy ‘Valencia’ orange (Citrus sinensis L. Osbeck) and in two cold-hardy genotypes, ‘Trifoliata’ orange (Poncirus trifoliata L. Raf.) and ‘FF-6-7-2’ citrus hybrid (Citrus ichangensis Swing. X Fortunella margarita Lour. Swing.). Soluble protein extracted from leaves and analyzed by electrophoresis showed significant differences in composition among the three citrus genotypes. A 12 kD polypeptide with unknown function was present in large quantity in ‘Valencia’, but not in the other two genotypes. Probing with antibodies specific for Rubisco and PEPCase indicated that ‘Trifoliata’ had two polypeptides of Rubisco small subunit (13 and 14 kD) whereas ‘Valencia’ and ‘FF-6-7-2’ had only one (14 kD). Cold acclimation differentially altered the expression of the two carboxylases. In all three genotypes, cold acclimation resulted in increased amount of Rubisco large subunit, but decreased amount of the small subunit. However, the amount of PEPCase was substantially enhanced by cold acclimation in the cold-hardy genotypes (‘FF-6-7-2’ and ‘Trifoliata’), but was largely reduced by the cold treatment in the moderately cold-hardy genotype (‘Valencia’). The enhanced expression of PEPCase during cold acclimation may be important for the induction of freezing tolerance in citrus.</abstract><qualityIndicators><score>5.219</score><pdfVersion>1.3</pdfVersion><pdfPageSize>504 x 692 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>9</keywordCount><abstractCharCount>1466</abstractCharCount><pdfWordCount>2783</pdfWordCount><pdfCharCount>18184</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>203</abstractWordCount></qualityIndicators><title>Cold-induced changes in ribulose 1,5-bisphosphate carboxylase-oxygenase and phosphoenolpyruvate carboxylase in citrus</title><pii><json:string>0098-8472(94)00044-6</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>76</volume><pages><last>4354</last><first>4350</first></pages><language><json:string>unknown</json:string></language><title>Proc. Natn. Acad. Sci. 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Osbeck) and in two cold-hardy genotypes, ‘Trifoliata’ orange (Poncirus trifoliata L. Raf.) and ‘FF-6-7-2’ citrus hybrid (Citrus ichangensis Swing. X Fortunella margarita Lour. Swing.). Soluble protein extracted from leaves and analyzed by electrophoresis showed significant differences in composition among the three citrus genotypes. A 12 kD polypeptide with unknown function was present in large quantity in ‘Valencia’, but not in the other two genotypes. Probing with antibodies specific for Rubisco and PEPCase indicated that ‘Trifoliata’ had two polypeptides of Rubisco small subunit (13 and 14 kD) whereas ‘Valencia’ and ‘FF-6-7-2’ had only one (14 kD). Cold acclimation differentially altered the expression of the two carboxylases. In all three genotypes, cold acclimation resulted in increased amount of Rubisco large subunit, but decreased amount of the small subunit. However, the amount of PEPCase was substantially enhanced by cold acclimation in the cold-hardy genotypes (‘FF-6-7-2’ and ‘Trifoliata’), but was largely reduced by the cold treatment in the moderately cold-hardy genotype (‘Valencia’). The enhanced expression of PEPCase during cold acclimation may be important for the induction of freezing tolerance in citrus.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Citrus</term></item><item><term>cold acclimation</term></item><item><term>protein expression</term></item></list></keywords></textClass><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>LSU</term><term>large subunit</term></item><item><term>PEPCase</term><term>phosphoenolpyruvate carboxylase</term></item><item><term>PPFD</term><term>photosynthetic photon flux density</term></item><item><term>Rubisco</term><term>ribulose 1,5-bisphosphate carboxylase-oxygenase</term></item><item><term>SDS-PAGE</term><term>sodium dodecyl sulfate-polyacrylamide gel electrophoresis</term></item><item><term>SSU</term><term>small subunit</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/84D93BE3493DF9413C2B1EFE345D21B8D24E6210/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="fla"><item-info><jid>EEB</jid><aid>94000446</aid><ce:pii>0098-8472(94)00044-6</ce:pii><ce:doi>10.1016/0098-8472(94)00044-6</ce:doi><ce:copyright type="unknown" year="1995">Elsevier Science Ltd</ce:copyright></item-info><head><ce:article-footnote><ce:note-para>Disclaimer — Mention of a trademark, warranty, proprietary product or vendor does not constitute a guarantee by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable.</ce:note-para></ce:article-footnote><ce:title>Cold-induced changes in ribulose 1,5-bisphosphate carboxylase-oxygenase and phosphoenolpyruvate carboxylase in citrus</ce:title><ce:author-group><ce:author><ce:given-name>Joseph C.V.</ce:given-name><ce:surname>Vu</ce:surname><ce:cross-ref refid="aff1"><ce:sup>*</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Sanjay K.</ce:given-name><ce:surname>Gupta</ce:surname><ce:cross-ref refid="aff2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>George</ce:given-name><ce:surname>Yelenosky</ce:surname><ce:cross-ref refid="aff3"><ce:sup>‡</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Maurice S.B.</ce:given-name><ce:surname>Ku</ce:surname><ce:cross-ref refid="aff2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:label>*</ce:label><ce:textfn>U.S. Department of Agriculture, Agricultural Research Service, Agronomy Physiology Laboratory, Building 164, University of Florida, Gainesville, FL 32611-0840, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="aff2"><ce:label>†</ce:label><ce:textfn>Botany Department, Washington State University, Pullman, WA 99164-4238, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="aff3"><ce:label>‡</ce:label><ce:textfn>U.S. Horticultural Research Laboratory, 2120 Camden Road, Orlando, FL 32803-1419, U.S.A.</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="16" month="5" year="1994"></ce:date-received><ce:date-accepted day="13" month="7" year="1994"></ce:date-accepted><ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The effects of cold acclimation on ribulose 1,5-bisphosphate carboxylase-oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPCase) were examined in leaves of moderately cold-hardy ‘Valencia’ orange (<ce:italic>Citrus sinensis</ce:italic> L. Osbeck) and in two cold-hardy genotypes, ‘Trifoliata’ orange (<ce:italic>Poncirus trifoliata</ce:italic> L. Raf.) and ‘FF-6-7-2’ citrus hybrid (<ce:italic>Citrus ichangensis</ce:italic> Swing. X <ce:italic>Fortunella margarita</ce:italic> Lour. Swing.). Soluble protein extracted from leaves and analyzed by electrophoresis showed significant differences in composition among the three citrus genotypes. A 12 kD polypeptide with unknown function was present in large quantity in ‘Valencia’, but not in the other two genotypes. Probing with antibodies specific for Rubisco and PEPCase indicated that ‘Trifoliata’ had two polypeptides of Rubisco small subunit (13 and 14 kD) whereas ‘Valencia’ and ‘FF-6-7-2’ had only one (14 kD). Cold acclimation differentially altered the expression of the two carboxylases. In all three genotypes, cold acclimation resulted in increased amount of Rubisco large subunit, but decreased amount of the small subunit. However, the amount of PEPCase was substantially enhanced by cold acclimation in the cold-hardy genotypes (‘FF-6-7-2’ and ‘Trifoliata’), but was largely reduced by the cold treatment in the moderately cold-hardy genotype (‘Valencia’). The enhanced expression of PEPCase during cold acclimation may be important for the induction of freezing tolerance in citrus.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Citrus</ce:text></ce:keyword><ce:keyword><ce:text>cold acclimation</ce:text></ce:keyword><ce:keyword><ce:text>protein expression</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr" xml:lang="en"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>LSU</ce:text><ce:keyword><ce:text>large subunit</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PEPCase</ce:text><ce:keyword><ce:text>phosphoenolpyruvate carboxylase</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PPFD</ce:text><ce:keyword><ce:text>photosynthetic photon flux density</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Rubisco</ce:text><ce:keyword><ce:text>ribulose 1,5-bisphosphate carboxylase-oxygenase</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>SDS-PAGE</ce:text><ce:keyword><ce:text>sodium dodecyl sulfate-polyacrylamide gel electrophoresis</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>SSU</ce:text><ce:keyword><ce:text>small subunit</ce:text></ce:keyword></ce:keyword></ce:keywords></head><tail><ce:bibliography><ce:section-title>References</ce:section-title><ce:bibliography-sec><ce:bib-reference id="bib1"><ce:label>1.</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Cattivelli</ce:surname><ce:given-name>L.</ce:given-name></sb:author><sb:author><ce:surname>Bartels</ce:surname><ce:given-name>D.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Biochemistry and molecular biology of cold-inducible enzymes and proteins in higher plants</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:editors><sb:editor><ce:surname>Wray</ce:surname><ce:given-name>J.L.</ce:given-name></sb:editor></sb:editors><sb:title><sb:maintitle>Inducible plant proteins: their biochemistry and molecular biology</sb:maintitle></sb:title><sb:date>1992</sb:date><sb:publisher><sb:name>Cambridge University Press</sb:name><sb:location>Cambridge</sb:location></sb:publisher></sb:edited-book><sb:pages><sb:first-page>267</sb:first-page><sb:last-page>288</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib2"><ce:label>2.</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Chabot</ce:surname><ce:given-name>B.F.</ce:given-name></sb:author><sb:author><ce:surname>Chabot</ce:surname><ce:given-name>J.F.</ce:given-name></sb:author><sb:author><ce:surname>Billings</ce:surname><ce:given-name>W.D.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Ribulose-1,5-diphosphate carboxylase activity in artic and alpine populations of <ce:italic>Oxyria digyna</ce:italic></sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Photosynthetica</sb:maintitle></sb:title><sb:volume-nr>6</sb:volume-nr></sb:series><sb:date>1972</sb:date></sb:issue><sb:pages><sb:first-page>364</sb:first-page><sb:last-page>369</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib3"><ce:label>3.</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Chu</ce:surname><ce:given-name>C.</ce:given-name></sb:author><sb:author><ce:surname>Dai</ce:surname><ce:given-name>Z.</ce:given-name></sb:author><sb:author><ce:surname>Ku</ce:surname><ce:given-name>M.S.B.</ce:given-name></sb:author><sb:author><ce:surname>Edwards</ce:surname><ce:given-name>G.E.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Induction of crassulacean acid metabolism in the facultative halophyte <ce:italic>Mesembryanthemum crystallinum</ce:italic> by ABA</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Pl. 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Soc.</sb:maintitle></sb:title><sb:volume-nr>103</sb:volume-nr></sb:series></sb:book-series><sb:date>1990</sb:date></sb:edited-book><sb:pages><sb:first-page>67</sb:first-page><sb:last-page>71</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cold-induced changes in ribulose 1,5-bisphosphate carboxylase-oxygenase and phosphoenolpyruvate carboxylase in citrus</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Cold-induced changes in ribulose 1,5-bisphosphate carboxylase-oxygenase and phosphoenolpyruvate carboxylase in citrus</title></titleInfo><name type="personal"><namePart type="given">Joseph C.V.</namePart><namePart type="family">Vu</namePart><affiliation>U.S. Department of Agriculture, Agricultural Research Service, Agronomy Physiology Laboratory, Building 164, University of Florida, Gainesville, FL 32611-0840, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sanjay K.</namePart><namePart type="family">Gupta</namePart><affiliation>Botany Department, Washington State University, Pullman, WA 99164-4238, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">George</namePart><namePart type="family">Yelenosky</namePart><affiliation>U.S. Horticultural Research Laboratory, 2120 Camden Road, Orlando, FL 32803-1419, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Maurice S.B.</namePart><namePart type="family">Ku</namePart><affiliation>Botany Department, Washington State University, Pullman, WA 99164-4238, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Abstract: The effects of cold acclimation on ribulose 1,5-bisphosphate carboxylase-oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPCase) were examined in leaves of moderately cold-hardy ‘Valencia’ orange (Citrus sinensis L. Osbeck) and in two cold-hardy genotypes, ‘Trifoliata’ orange (Poncirus trifoliata L. Raf.) and ‘FF-6-7-2’ citrus hybrid (Citrus ichangensis Swing. X Fortunella margarita Lour. Swing.). Soluble protein extracted from leaves and analyzed by electrophoresis showed significant differences in composition among the three citrus genotypes. A 12 kD polypeptide with unknown function was present in large quantity in ‘Valencia’, but not in the other two genotypes. Probing with antibodies specific for Rubisco and PEPCase indicated that ‘Trifoliata’ had two polypeptides of Rubisco small subunit (13 and 14 kD) whereas ‘Valencia’ and ‘FF-6-7-2’ had only one (14 kD). Cold acclimation differentially altered the expression of the two carboxylases. In all three genotypes, cold acclimation resulted in increased amount of Rubisco large subunit, but decreased amount of the small subunit. However, the amount of PEPCase was substantially enhanced by cold acclimation in the cold-hardy genotypes (‘FF-6-7-2’ and ‘Trifoliata’), but was largely reduced by the cold treatment in the moderately cold-hardy genotype (‘Valencia’). The enhanced expression of PEPCase during cold acclimation may be important for the induction of freezing tolerance in citrus.</abstract><note>Disclaimer — Mention of a trademark, warranty, proprietary product or vendor does not constitute a guarantee by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable.</note><subject lang="en"><genre>Keywords</genre><topic>Citrus</topic><topic>cold acclimation</topic><topic>protein expression</topic></subject><subject lang="en"><genre>Abbreviations</genre><topic>LSU : large subunit</topic><topic>PEPCase : phosphoenolpyruvate carboxylase</topic><topic>PPFD : photosynthetic photon flux density</topic><topic>Rubisco : ribulose 1,5-bisphosphate carboxylase-oxygenase</topic><topic>SDS-PAGE : sodium dodecyl sulfate-polyacrylamide gel electrophoresis</topic><topic>SSU : small subunit</topic></subject><relatedItem type="host"><titleInfo><title>Environmental and Experimental Botany</title></titleInfo><titleInfo type="abbreviated"><title>EEB</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199501</dateIssued></originInfo><identifier type="ISSN">0098-8472</identifier><identifier type="PII">S0098-8472(00)X0007-X</identifier><part><date>199501</date><detail type="volume"><number>35</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>113</end></extent><extent unit="pages"><start>25</start><end>31</end></extent></part></relatedItem><identifier type="istex">84D93BE3493DF9413C2B1EFE345D21B8D24E6210</identifier><identifier type="DOI">10.1016/0098-8472(94)00044-6</identifier><identifier type="PII">0098-8472(94)00044-6</identifier><identifier type="ArticleID">94000446</identifier><accessCondition type="use and reproduction" contentType="copyright">©1995 Elsevier Science Ltd</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Ltd, ©1995</recordOrigin></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/84D93BE3493DF9413C2B1EFE345D21B8D24E6210/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">ENVIRONMENTAL SCIENCES</classCode><classCode scheme="WOS">PLANT SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
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