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Morphological and molecular characterization of selected Ramaria mycorrhizae.

Identifieur interne : 003532 ( Main/Exploration ); précédent : 003531; suivant : 003533

Morphological and molecular characterization of selected Ramaria mycorrhizae.

Auteurs : Eduardo R. Nouhra [Argentine] ; Thomas R. Horton ; Efren Cazares ; Michael Castellano

Source :

RBID : pubmed:14745631

Descripteurs français

English descriptors

Abstract

Ramaria species are conspicuous mycorrhizal symbionts of conifers in the Pacific Northwest. Here we collected and identified sporocarps and associated ectomycorrhizae of Ramaria acrisiccescens Marr & Stuntz, R. cyaneigranosa Marr & Stuntz, R. sandaracina Marr & Stuntz, R. celerivirescens Marr & Stuntz, and R. flavobrunnescens var. aromatica Marr & Stuntz. An internal transcribed spacer (ITS)- restriction fragment length polymorphism pattern was observed for each of the Ramaria species and used as a diagnostic tool to support the identification of mycorrhizae occurring in mats below the sporocarps. We provide a description of ectomycorrhizae of Ramaria, which exhibit similar macro- and microscopic characteristics such as ramification pattern, coloration, abundance of mycelial strands and emanating hyphae, mantle morphology and chemical reactions of mantle and mycelial strands with KOH, FeSO4 and Melzer's reagent. Sequences of the ITS region for each of the species are deposited in the GenBank.

DOI: 10.1007/s00572-004-0294-5
PubMed: 14745631


Affiliations:


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<div type="abstract" xml:lang="en">Ramaria species are conspicuous mycorrhizal symbionts of conifers in the Pacific Northwest. Here we collected and identified sporocarps and associated ectomycorrhizae of Ramaria acrisiccescens Marr & Stuntz, R. cyaneigranosa Marr & Stuntz, R. sandaracina Marr & Stuntz, R. celerivirescens Marr & Stuntz, and R. flavobrunnescens var. aromatica Marr & Stuntz. An internal transcribed spacer (ITS)- restriction fragment length polymorphism pattern was observed for each of the Ramaria species and used as a diagnostic tool to support the identification of mycorrhizae occurring in mats below the sporocarps. We provide a description of ectomycorrhizae of Ramaria, which exhibit similar macro- and microscopic characteristics such as ramification pattern, coloration, abundance of mycelial strands and emanating hyphae, mantle morphology and chemical reactions of mantle and mycelial strands with KOH, FeSO4 and Melzer's reagent. Sequences of the ITS region for each of the species are deposited in the GenBank.</div>
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