Isolation and phenotypic characterization of Lotus japonicus mutants specifically defective in arbuscular mycorrhizal formation.
Identifieur interne : 001890 ( Main/Exploration ); précédent : 001889; suivant : 001891Isolation and phenotypic characterization of Lotus japonicus mutants specifically defective in arbuscular mycorrhizal formation.
Auteurs : Tomoko Kojima [Japon] ; Katsuharu Saito ; Hirosuke Oba ; Yuma Yoshida ; Junya Terasawa ; Yosuke Umehara ; Norio Suganuma ; Masayoshi Kawaguchi ; Ryo OhtomoSource :
- Plant & cell physiology [ 1471-9053 ] ; 2014.
Descripteurs français
- KwdFr :
- Cartographie chromosomique (MeSH), Chromosomes de plante (génétique), Données de séquences moléculaires (MeSH), Hyphae (génétique), Hyphae (physiologie), Interactions hôte-pathogène (génétique), Loteae (génétique), Loteae (microbiologie), Mesorhizobium (physiologie), Mesorhizobium (ultrastructure), Microscopie confocale (MeSH), Microscopie électronique à transmission (MeSH), Mutagenèse (effets des médicaments et des substances chimiques), Mutagènes (toxicité), Mutation (MeSH), Mycorhizes (génétique), Mycorhizes (physiologie), Méthanesulfonate d'éthyle (toxicité), Nodulation racinaire (génétique), Nodules racinaires de plante (génétique), Nodules racinaires de plante (microbiologie), Phénotype (MeSH), RT-PCR (MeSH), Racines de plante (génétique), Racines de plante (microbiologie), Régulation de l'expression des gènes fongiques (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Symbiose (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Mutagenèse.
- génétique : Chromosomes de plante, Hyphae, Interactions hôte-pathogène, Loteae, Mycorhizes, Nodulation racinaire, Nodules racinaires de plante, Racines de plante.
- microbiologie : Loteae, Nodules racinaires de plante, Racines de plante.
- physiologie : Hyphae, Mesorhizobium, Mycorhizes.
- toxicité : Mutagènes, Méthanesulfonate d'éthyle.
- ultrastructure : Cartographie chromosomique, Données de séquences moléculaires, Mesorhizobium, Microscopie confocale, Microscopie électronique à transmission, Mutation, Phénotype, RT-PCR, Régulation de l'expression des gènes fongiques, Régulation de l'expression des gènes végétaux, Symbiose.
English descriptors
- KwdEn :
- Chromosome Mapping (MeSH), Chromosomes, Plant (genetics), Ethyl Methanesulfonate (toxicity), Gene Expression Regulation, Fungal (MeSH), Gene Expression Regulation, Plant (MeSH), Host-Pathogen Interactions (genetics), Hyphae (genetics), Hyphae (physiology), Lotus (genetics), Lotus (microbiology), Mesorhizobium (physiology), Mesorhizobium (ultrastructure), Microscopy, Confocal (MeSH), Microscopy, Electron, Transmission (MeSH), Molecular Sequence Data (MeSH), Mutagenesis (drug effects), Mutagens (toxicity), Mutation (MeSH), Mycorrhizae (genetics), Mycorrhizae (physiology), Phenotype (MeSH), Plant Root Nodulation (genetics), Plant Roots (genetics), Plant Roots (microbiology), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Root Nodules, Plant (genetics), Root Nodules, Plant (microbiology), Symbiosis (MeSH).
- MESH :
- chemical , toxicity : Ethyl Methanesulfonate, Mutagens.
- drug effects : Mutagenesis.
- genetics : Chromosomes, Plant, Host-Pathogen Interactions, Hyphae, Lotus, Mycorrhizae, Plant Root Nodulation, Plant Roots, Root Nodules, Plant.
- microbiology : Lotus, Plant Roots, Root Nodules, Plant.
- physiology : Hyphae, Mesorhizobium, Mycorrhizae.
- ultrastructure : Mesorhizobium.
- Chromosome Mapping, Gene Expression Regulation, Fungal, Gene Expression Regulation, Plant, Microscopy, Confocal, Microscopy, Electron, Transmission, Molecular Sequence Data, Mutation, Phenotype, Reverse Transcriptase Polymerase Chain Reaction, Symbiosis.
Abstract
Several symbiotic mutants of legume plants defective in nodulation have also been shown to be mutants related to arbuscular mycorrhizal (AM) symbiosis. The origin of the AM symbiosis can be traced back to the early land plants. It has therefore been postulated that the older system of AM symbiosis was partially incorporated into the newer system of legume-rhizobium symbiosis. To unravel the genetic basis of the establishment of AM symbiosis, we screened about 34,000 plants derived from ethyl methanesulfonate (EMS)-mutagenized Lotus japonicus seeds by microscopic observation. As a result, three lines (ME778, ME966 and ME2329) were isolated as AM-specific mutants that exhibit clear AM-defective phenotypes but form normal effective root nodules with rhizobial infection. In the ME2329 mutant, AM fungi spread their hyphae into the intercellular space of the cortex and formed trunk hyphae in the cortical cells, but the development of fine branches in the arbuscules was arrested. The ME2329 mutant carried a nonsense mutation in the STR-homolog gene, implying that the line may be an str mutant in L. japonicus. On the ME778 and ME966 mutant roots, the entry of AM fungal hyphae was blocked between two adjacent epidermal cells. Occasionally, hyphal colonization accompanied by arbuscules was observed in the two mutants. The genes responsible for the ME778 and ME966 mutants were independently located on chromosome 2. These results suggest that the ME778 and ME966 lines are symbiotic mutants involved in the early stage of AM formation in L. japonicus.
DOI: 10.1093/pcp/pcu024
PubMed: 24492255
Affiliations:
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Science, Nasushiobara, Tochigi, 329-2793 Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Agriculture and Food Research Organization (NARO) Institute of Livestock and Grassland Science, Nasushiobara, Tochigi</wicri:regionArea><wicri:noRegion>Tochigi</wicri:noRegion></affiliation></author><author><name sortKey="Saito, Katsuharu" sort="Saito, Katsuharu" uniqKey="Saito K" first="Katsuharu" last="Saito">Katsuharu Saito</name></author><author><name sortKey="Oba, Hirosuke" sort="Oba, Hirosuke" uniqKey="Oba H" first="Hirosuke" last="Oba">Hirosuke Oba</name></author><author><name sortKey="Yoshida, Yuma" sort="Yoshida, Yuma" uniqKey="Yoshida Y" first="Yuma" last="Yoshida">Yuma Yoshida</name></author><author><name sortKey="Terasawa, Junya" sort="Terasawa, Junya" uniqKey="Terasawa J" first="Junya" last="Terasawa">Junya Terasawa</name></author><author><name sortKey="Umehara, Yosuke" sort="Umehara, Yosuke" uniqKey="Umehara Y" first="Yosuke" 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(MeSH)</term><term>Host-Pathogen Interactions (genetics)</term><term>Hyphae (genetics)</term><term>Hyphae (physiology)</term><term>Lotus (genetics)</term><term>Lotus (microbiology)</term><term>Mesorhizobium (physiology)</term><term>Mesorhizobium (ultrastructure)</term><term>Microscopy, Confocal (MeSH)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mutagenesis (drug effects)</term><term>Mutagens (toxicity)</term><term>Mutation (MeSH)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (physiology)</term><term>Phenotype (MeSH)</term><term>Plant Root Nodulation (genetics)</term><term>Plant Roots (genetics)</term><term>Plant Roots (microbiology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Root Nodules, Plant (genetics)</term><term>Root Nodules, Plant (microbiology)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cartographie chromosomique (MeSH)</term><term>Chromosomes de plante (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Hyphae (génétique)</term><term>Hyphae (physiologie)</term><term>Interactions hôte-pathogène (génétique)</term><term>Loteae (génétique)</term><term>Loteae (microbiologie)</term><term>Mesorhizobium (physiologie)</term><term>Mesorhizobium (ultrastructure)</term><term>Microscopie confocale (MeSH)</term><term>Microscopie électronique à transmission (MeSH)</term><term>Mutagenèse (effets des médicaments et des substances chimiques)</term><term>Mutagènes (toxicité)</term><term>Mutation (MeSH)</term><term>Mycorhizes (génétique)</term><term>Mycorhizes (physiologie)</term><term>Méthanesulfonate d'éthyle (toxicité)</term><term>Nodulation racinaire (génétique)</term><term>Nodules racinaires de plante (génétique)</term><term>Nodules racinaires de plante (microbiologie)</term><term>Phénotype (MeSH)</term><term>RT-PCR (MeSH)</term><term>Racines de plante (génétique)</term><term>Racines de plante (microbiologie)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Symbiose (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Ethyl Methanesulfonate</term><term>Mutagens</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Mutagenesis</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Mutagenèse</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Plant</term><term>Host-Pathogen Interactions</term><term>Hyphae</term><term>Lotus</term><term>Mycorrhizae</term><term>Plant Root Nodulation</term><term>Plant Roots</term><term>Root Nodules, Plant</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chromosomes de plante</term><term>Hyphae</term><term>Interactions 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xml:lang="en"><term>Mesorhizobium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromosome Mapping</term><term>Gene Expression Regulation, Fungal</term><term>Gene Expression Regulation, Plant</term><term>Microscopy, Confocal</term><term>Microscopy, Electron, Transmission</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Phenotype</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Symbiosis</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Cartographie chromosomique</term><term>Données de séquences moléculaires</term><term>Mesorhizobium</term><term>Microscopie confocale</term><term>Microscopie électronique à transmission</term><term>Mutation</term><term>Phénotype</term><term>RT-PCR</term><term>Régulation de l'expression des gènes fongiques</term><term>Régulation de l'expression des gènes végétaux</term><term>Symbiose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Several symbiotic mutants of legume plants defective in nodulation have also been shown to be mutants related to arbuscular mycorrhizal (AM) symbiosis. The origin of the AM symbiosis can be traced back to the early land plants. It has therefore been postulated that the older system of AM symbiosis was partially incorporated into the newer system of legume-rhizobium symbiosis. To unravel the genetic basis of the establishment of AM symbiosis, we screened about 34,000 plants derived from ethyl methanesulfonate (EMS)-mutagenized Lotus japonicus seeds by microscopic observation. As a result, three lines (ME778, ME966 and ME2329) were isolated as AM-specific mutants that exhibit clear AM-defective phenotypes but form normal effective root nodules with rhizobial infection. In the ME2329 mutant, AM fungi spread their hyphae into the intercellular space of the cortex and formed trunk hyphae in the cortical cells, but the development of fine branches in the arbuscules was arrested. The ME2329 mutant carried a nonsense mutation in the STR-homolog gene, implying that the line may be an str mutant in L. japonicus. On the ME778 and ME966 mutant roots, the entry of AM fungal hyphae was blocked between two adjacent epidermal cells. Occasionally, hyphal colonization accompanied by arbuscules was observed in the two mutants. The genes responsible for the ME778 and ME966 mutants were independently located on chromosome 2. These results suggest that the ME778 and ME966 lines are symbiotic mutants involved in the early stage of AM formation in L. japonicus. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24492255</PMID><DateCompleted><Year>2015</Year><Month>02</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1471-9053</ISSN><JournalIssue CitedMedium="Internet"><Volume>55</Volume><Issue>5</Issue><PubDate><Year>2014</Year><Month>May</Month></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>Isolation and phenotypic characterization of Lotus japonicus mutants specifically defective in arbuscular mycorrhizal formation.</ArticleTitle><Pagination><MedlinePgn>928-41</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/pcp/pcu024</ELocationID><Abstract><AbstractText>Several symbiotic mutants of legume plants defective in nodulation have also been shown to be mutants related to arbuscular mycorrhizal (AM) symbiosis. The origin of the AM symbiosis can be traced back to the early land plants. It has therefore been postulated that the older system of AM symbiosis was partially incorporated into the newer system of legume-rhizobium symbiosis. To unravel the genetic basis of the establishment of AM symbiosis, we screened about 34,000 plants derived from ethyl methanesulfonate (EMS)-mutagenized Lotus japonicus seeds by microscopic observation. As a result, three lines (ME778, ME966 and ME2329) were isolated as AM-specific mutants that exhibit clear AM-defective phenotypes but form normal effective root nodules with rhizobial infection. In the ME2329 mutant, AM fungi spread their hyphae into the intercellular space of the cortex and formed trunk hyphae in the cortical cells, but the development of fine branches in the arbuscules was arrested. The ME2329 mutant carried a nonsense mutation in the STR-homolog gene, implying that the line may be an str mutant in L. japonicus. On the ME778 and ME966 mutant roots, the entry of AM fungal hyphae was blocked between two adjacent epidermal cells. Occasionally, hyphal colonization accompanied by arbuscules was observed in the two mutants. The genes responsible for the ME778 and ME966 mutants were independently located on chromosome 2. These results suggest that the ME778 and ME966 lines are symbiotic mutants involved in the early stage of AM formation in L. japonicus. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kojima</LastName><ForeName>Tomoko</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>National Agriculture and Food Research Organization (NARO) Institute of Livestock and Grassland Science, Nasushiobara, Tochigi, 329-2793 Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saito</LastName><ForeName>Katsuharu</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Oba</LastName><ForeName>Hirosuke</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Yoshida</LastName><ForeName>Yuma</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Terasawa</LastName><ForeName>Junya</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Umehara</LastName><ForeName>Yosuke</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Suganuma</LastName><ForeName>Norio</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Kawaguchi</LastName><ForeName>Masayoshi</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Ohtomo</LastName><ForeName>Ryo</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AB830530</AccessionNumber><AccessionNumber>AB830531</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>02</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Cell Physiol</MedlineTA><NlmUniqueID>9430925</NlmUniqueID><ISSNLinking>0032-0781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009153">Mutagens</NameOfSubstance></Chemical><Chemical><RegistryNumber>9H154DI0UP</RegistryNumber><NameOfSubstance UI="D005020">Ethyl Methanesulfonate</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032461" MajorTopicYN="N">Chromosomes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005020" MajorTopicYN="N">Ethyl Methanesulfonate</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000070116" MajorTopicYN="N">Lotus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059128" MajorTopicYN="N">Mesorhizobium</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016296" MajorTopicYN="N">Mutagenesis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009153" MajorTopicYN="N">Mutagens</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="Y">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055170" MajorTopicYN="N">Plant Root Nodulation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053204" MajorTopicYN="N">Root Nodules, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arbuscular mycorrhizal fungi</Keyword><Keyword MajorTopicYN="N">EMS</Keyword><Keyword MajorTopicYN="N">Lotus japonicus</Keyword><Keyword MajorTopicYN="N">Symbiotic mutants</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>2</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24492255</ArticleId><ArticleId IdType="pii">pcu024</ArticleId><ArticleId IdType="doi">10.1093/pcp/pcu024</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Kawaguchi, Masayoshi" sort="Kawaguchi, Masayoshi" uniqKey="Kawaguchi M" first="Masayoshi" last="Kawaguchi">Masayoshi Kawaguchi</name><name sortKey="Oba, Hirosuke" sort="Oba, Hirosuke" uniqKey="Oba H" first="Hirosuke" last="Oba">Hirosuke Oba</name><name sortKey="Ohtomo, Ryo" sort="Ohtomo, Ryo" uniqKey="Ohtomo R" first="Ryo" last="Ohtomo">Ryo Ohtomo</name><name sortKey="Saito, Katsuharu" sort="Saito, Katsuharu" uniqKey="Saito K" first="Katsuharu" last="Saito">Katsuharu Saito</name><name sortKey="Suganuma, Norio" sort="Suganuma, Norio" uniqKey="Suganuma N" first="Norio" last="Suganuma">Norio Suganuma</name><name sortKey="Terasawa, Junya" sort="Terasawa, Junya" uniqKey="Terasawa J" first="Junya" last="Terasawa">Junya Terasawa</name><name sortKey="Umehara, Yosuke" sort="Umehara, Yosuke" uniqKey="Umehara Y" first="Yosuke" last="Umehara">Yosuke Umehara</name><name sortKey="Yoshida, Yuma" sort="Yoshida, Yuma" uniqKey="Yoshida Y" first="Yuma" last="Yoshida">Yuma Yoshida</name></noCountry><country name="Japon"><noRegion><name sortKey="Kojima, Tomoko" sort="Kojima, Tomoko" uniqKey="Kojima T" first="Tomoko" last="Kojima">Tomoko Kojima</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
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|clé= pubmed:24492255
|texte= Isolation and phenotypic characterization of Lotus japonicus mutants specifically defective in arbuscular mycorrhizal formation.
}}
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