The sucrose transporter SlSUT2 from tomato interacts with brassinosteroid functioning and affects arbuscular mycorrhiza formation.
Identifieur interne : 001755 ( Main/Exploration ); précédent : 001754; suivant : 001756The sucrose transporter SlSUT2 from tomato interacts with brassinosteroid functioning and affects arbuscular mycorrhiza formation.
Auteurs : Michael Bitterlich [Allemagne] ; Undine Krügel ; Katja Boldt-Burisch ; Philipp Franken ; Christina KühnSource :
- The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2014.
Descripteurs français
- KwdFr :
- Lycopersicon esculentum (microbiologie), Lycopersicon esculentum (métabolisme), Mycorhizes (physiologie), Protéines de transport membranaire (génétique), Protéines de transport membranaire (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (génétique), Régulation de l'expression des gènes végétaux (physiologie), Symbiose (MeSH).
- MESH :
- génétique : Protéines de transport membranaire, Protéines végétales, Régulation de l'expression des gènes végétaux.
- microbiologie : Lycopersicon esculentum.
- métabolisme : Lycopersicon esculentum, Protéines de transport membranaire, Protéines végétales.
- physiologie : Mycorhizes, Régulation de l'expression des gènes végétaux.
- Symbiose.
English descriptors
- KwdEn :
- Gene Expression Regulation, Plant (genetics), Gene Expression Regulation, Plant (physiology), Lycopersicon esculentum (metabolism), Lycopersicon esculentum (microbiology), Membrane Transport Proteins (genetics), Membrane Transport Proteins (metabolism), Mycorrhizae (physiology), Plant Proteins (genetics), Plant Proteins (metabolism), Symbiosis (MeSH).
- MESH :
- chemical , genetics : Membrane Transport Proteins, Plant Proteins.
- genetics : Gene Expression Regulation, Plant.
- metabolism : Lycopersicon esculentum, Membrane Transport Proteins, Plant Proteins.
- microbiology : Lycopersicon esculentum.
- physiology : Gene Expression Regulation, Plant, Mycorrhizae.
- Symbiosis.
Abstract
Mycorrhizal plants benefit from the fungal partners by getting better access to soil nutrients. In exchange, the plant supplies carbohydrates to the fungus. The additional carbohydrate demand in mycorrhizal plants was shown to be balanced partially by higher CO2 assimilation and increased C metabolism in shoots and roots. In order to test the role of sucrose transport for fungal development in arbuscular mycorrhizal (AM) tomato, transgenic plants with down-regulated expression of three sucrose transporter genes were analysed. Plants that carried an antisense construct of SlSUT2 (SlSUT2as) repeatedly exhibited increased mycorrhizal colonization and the positive effect of plants to mycorrhiza was abolished. Grafting experiments between transgenic and wild-type rootstocks and scions indicated that mainly the root-specific function of SlSUT2 has an impact on colonization of tomato roots with the AM fungus. Localization of SISUT2 to the periarbuscular membrane indicates a role in back transport of sucrose from the periarbuscular matrix into the plant cell thereby affecting hyphal development. Screening of an expression library for SlSUT2-interacting proteins revealed interactions with candidates involved in brassinosteroid (BR) signaling or biosynthesis. Interaction of these candidates with SlSUT2 was confirmed by bimolecular fluorescence complementation. Tomato mutants defective in BR biosynthesis were analysed with respect to mycorrhizal symbiosis and showed indeed decreased mycorrhization. This finding suggests that BRs affect mycorrhizal infection and colonization. If the inhibitory effect of SlSUT2 on mycorrhizal growth involves components of BR synthesis and of the BR signaling pathway is discussed.
DOI: 10.1111/tpj.12515
PubMed: 24654931
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979, Großbeeren, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Plant Physiology Department, Humboldt University of Berlin, Philippstr. 13, Building 12, 10115, Berlin, Germany; Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979, Großbeeren</wicri:regionArea><wicri:noRegion>14979, Großbeeren</wicri:noRegion><wicri:noRegion>14979, Großbeeren</wicri:noRegion><wicri:noRegion>Großbeeren</wicri:noRegion></affiliation></author><author><name sortKey="Krugel, Undine" sort="Krugel, Undine" uniqKey="Krugel U" first="Undine" last="Krügel">Undine Krügel</name></author><author><name sortKey="Boldt Burisch, Katja" sort="Boldt Burisch, Katja" uniqKey="Boldt Burisch K" first="Katja" last="Boldt-Burisch">Katja Boldt-Burisch</name></author><author><name sortKey="Franken, Philipp" sort="Franken, Philipp" uniqKey="Franken P" first="Philipp" last="Franken">Philipp Franken</name></author><author><name sortKey="Kuhn, Christina" sort="Kuhn, Christina" uniqKey="Kuhn C" first="Christina" last="Kühn">Christina Kühn</name></author></analytic><series><title level="j">The Plant journal : for cell and molecular biology</title><idno type="eISSN">1365-313X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Regulation, Plant (genetics)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Lycopersicon esculentum (metabolism)</term><term>Lycopersicon esculentum (microbiology)</term><term>Membrane Transport Proteins (genetics)</term><term>Membrane Transport Proteins (metabolism)</term><term>Mycorrhizae (physiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Lycopersicon esculentum (microbiologie)</term><term>Lycopersicon esculentum (métabolisme)</term><term>Mycorhizes (physiologie)</term><term>Protéines de transport membranaire (génétique)</term><term>Protéines de transport membranaire (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (génétique)</term><term>Régulation de l'expression des gènes végétaux (physiologie)</term><term>Symbiose (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Transport Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de transport membranaire</term><term>Protéines végétales</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lycopersicon esculentum</term><term>Membrane Transport Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lycopersicon esculentum</term><term>Protéines de transport membranaire</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mycorhizes</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Symbiose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mycorrhizal plants benefit from the fungal partners by getting better access to soil nutrients. In exchange, the plant supplies carbohydrates to the fungus. The additional carbohydrate demand in mycorrhizal plants was shown to be balanced partially by higher CO2 assimilation and increased C metabolism in shoots and roots. In order to test the role of sucrose transport for fungal development in arbuscular mycorrhizal (AM) tomato, transgenic plants with down-regulated expression of three sucrose transporter genes were analysed. Plants that carried an antisense construct of SlSUT2 (SlSUT2as) repeatedly exhibited increased mycorrhizal colonization and the positive effect of plants to mycorrhiza was abolished. Grafting experiments between transgenic and wild-type rootstocks and scions indicated that mainly the root-specific function of SlSUT2 has an impact on colonization of tomato roots with the AM fungus. Localization of SISUT2 to the periarbuscular membrane indicates a role in back transport of sucrose from the periarbuscular matrix into the plant cell thereby affecting hyphal development. Screening of an expression library for SlSUT2-interacting proteins revealed interactions with candidates involved in brassinosteroid (BR) signaling or biosynthesis. Interaction of these candidates with SlSUT2 was confirmed by bimolecular fluorescence complementation. Tomato mutants defective in BR biosynthesis were analysed with respect to mycorrhizal symbiosis and showed indeed decreased mycorrhization. This finding suggests that BRs affect mycorrhizal infection and colonization. If the inhibitory effect of SlSUT2 on mycorrhizal growth involves components of BR synthesis and of the BR signaling pathway is discussed. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24654931</PMID><DateCompleted><Year>2015</Year><Month>01</Month><Day>09</Day></DateCompleted><DateRevised><Year>2014</Year><Month>05</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-313X</ISSN><JournalIssue CitedMedium="Internet"><Volume>78</Volume><Issue>5</Issue><PubDate><Year>2014</Year><Month>Jun</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>The sucrose transporter SlSUT2 from tomato interacts with brassinosteroid functioning and affects arbuscular mycorrhiza formation.</ArticleTitle><Pagination><MedlinePgn>877-89</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/tpj.12515</ELocationID><Abstract><AbstractText>Mycorrhizal plants benefit from the fungal partners by getting better access to soil nutrients. In exchange, the plant supplies carbohydrates to the fungus. The additional carbohydrate demand in mycorrhizal plants was shown to be balanced partially by higher CO2 assimilation and increased C metabolism in shoots and roots. In order to test the role of sucrose transport for fungal development in arbuscular mycorrhizal (AM) tomato, transgenic plants with down-regulated expression of three sucrose transporter genes were analysed. Plants that carried an antisense construct of SlSUT2 (SlSUT2as) repeatedly exhibited increased mycorrhizal colonization and the positive effect of plants to mycorrhiza was abolished. Grafting experiments between transgenic and wild-type rootstocks and scions indicated that mainly the root-specific function of SlSUT2 has an impact on colonization of tomato roots with the AM fungus. Localization of SISUT2 to the periarbuscular membrane indicates a role in back transport of sucrose from the periarbuscular matrix into the plant cell thereby affecting hyphal development. Screening of an expression library for SlSUT2-interacting proteins revealed interactions with candidates involved in brassinosteroid (BR) signaling or biosynthesis. Interaction of these candidates with SlSUT2 was confirmed by bimolecular fluorescence complementation. Tomato mutants defective in BR biosynthesis were analysed with respect to mycorrhizal symbiosis and showed indeed decreased mycorrhization. This finding suggests that BRs affect mycorrhizal infection and colonization. If the inhibitory effect of SlSUT2 on mycorrhizal growth involves components of BR synthesis and of the BR signaling pathway is discussed. </AbstractText><CopyrightInformation>© 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bitterlich</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Plant Physiology Department, Humboldt University of Berlin, Philippstr. 13, Building 12, 10115, Berlin, Germany; Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979, Großbeeren, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Krügel</LastName><ForeName>Undine</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Boldt-Burisch</LastName><ForeName>Katja</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Franken</LastName><ForeName>Philipp</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Kühn</LastName><ForeName>Christina</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>05</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026901">Membrane Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C078729">sucrose transport protein, plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D026901" MajorTopicYN="N">Membrane Transport Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Funneliformis mosseae</Keyword><Keyword MajorTopicYN="N">Solanum lycopersicum</Keyword><Keyword MajorTopicYN="N">arbuscular mycorrhiza</Keyword><Keyword MajorTopicYN="N">brassinosteroid signaling</Keyword><Keyword MajorTopicYN="N">brassinosteroid synthesis</Keyword><Keyword MajorTopicYN="N">protein-protein interactions</Keyword><Keyword MajorTopicYN="N">sucrose transporter</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>11</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>03</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>03</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>1</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24654931</ArticleId><ArticleId IdType="doi">10.1111/tpj.12515</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Boldt Burisch, Katja" sort="Boldt Burisch, Katja" uniqKey="Boldt Burisch K" first="Katja" last="Boldt-Burisch">Katja Boldt-Burisch</name><name sortKey="Franken, Philipp" sort="Franken, Philipp" uniqKey="Franken P" first="Philipp" last="Franken">Philipp Franken</name><name sortKey="Krugel, Undine" sort="Krugel, Undine" uniqKey="Krugel U" first="Undine" last="Krügel">Undine Krügel</name><name sortKey="Kuhn, Christina" sort="Kuhn, Christina" uniqKey="Kuhn C" first="Christina" last="Kühn">Christina Kühn</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Bitterlich, Michael" sort="Bitterlich, Michael" uniqKey="Bitterlich M" first="Michael" last="Bitterlich">Michael Bitterlich</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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