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Rapid determination of fungal colonization and arbuscule formation in roots of Medicago truncatula using real-time (RT) PCR.

Identifieur interne : 003630 ( Main/Curation ); précédent : 003629; suivant : 003631

Rapid determination of fungal colonization and arbuscule formation in roots of Medicago truncatula using real-time (RT) PCR.

Auteurs : Stanislav Isayenkov [Allemagne] ; Thomas Fester ; Bettina Hause

Source :

Journal of plant physiology [ 0176-1617 ] ; 2004.

RBID : pubmed:15658808

Descripteurs français

KwdFr :
- Champignons (physiologie), Medicago truncatula (croissance et développement), Medicago truncatula (génétique), Medicago truncatula (microbiologie), Mycorhizes (physiologie), Protéines de transport du phosphate (génétique), Protéines végétales (génétique), RT-PCR (méthodes).
MESH :
- croissance et développement : Medicago truncatula.
- génétique : Medicago truncatula, Protéines de transport du phosphate, Protéines végétales.
- microbiologie : Medicago truncatula.
- méthodes : RT-PCR.
- physiologie : Champignons, Mycorhizes.

English descriptors

KwdEn :
- Fungi (physiology), Medicago truncatula (genetics), Medicago truncatula (growth & development), Medicago truncatula (microbiology), Mycorrhizae (physiology), Phosphate Transport Proteins (genetics), Plant Proteins (genetics), Reverse Transcriptase Polymerase Chain Reaction (methods).
MESH :
- chemical , genetics : Phosphate Transport Proteins, Plant Proteins.
- genetics : Medicago truncatula.
- growth & development : Medicago truncatula.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- microbiology : Medicago truncatula.
- physiology : Fungi, Mycorrhizae.

Abstract

The quantifications of root colonization and symbiotic activity in the arbuscular mycorrhizal (AM) association of Medicago truncatula and Glomus intraradices were performed by quantitative polymerase chain reaction (real-time PCR). A strong correlation between fungal colonization of the root system and the amounts of fungal rDNA and rRNA were shown. In contrast, the transcript levels of the AM-specific phosphate transporter 4 from M. truncatula (MtPT4) correlate with arbuscule formation rather than with fungal colonization. These results suggest (i) that real-time PCR assay is a rapid, useful, and accurate method for the determination of arbuscular mycorrhizal features, (ii) that the amount of fungal rDNA or rRNA is a good parameter to estimate fungal colonization, and (iii) that it is necessary to evaluate the amount of other transcripts-like the MtPT4 transcript-to obtain additional information about the symbiotic state of the colonized root system.

DOI: 10.1016/j.jplph.2004.04.012
PubMed: 15658808

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Le document en format XML

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<term>Medicago truncatula (génétique)</term>
<term>Medicago truncatula (microbiologie)</term>
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<front><div type="abstract" xml:lang="en">The quantifications of root colonization and symbiotic activity in the arbuscular mycorrhizal (AM) association of Medicago truncatula and Glomus intraradices were performed by quantitative polymerase chain reaction (real-time PCR). A strong correlation between fungal colonization of the root system and the amounts of fungal rDNA and rRNA were shown. In contrast, the transcript levels of the AM-specific phosphate transporter 4 from M. truncatula (MtPT4) correlate with arbuscule formation rather than with fungal colonization. These results suggest (i) that real-time PCR assay is a rapid, useful, and accurate method for the determination of arbuscular mycorrhizal features, (ii) that the amount of fungal rDNA or rRNA is a good parameter to estimate fungal colonization, and (iii) that it is necessary to evaluate the amount of other transcripts-like the MtPT4 transcript-to obtain additional information about the symbiotic state of the colonized root system.</div>
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Rapid determination of fungal colonization and arbuscule formation in roots of Medicago truncatula using real-time (RT) PCR.

Rapid determination of fungal colonization and arbuscule formation in roots of Medicago truncatula using real-time (RT) PCR.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki