High specificity generally characterizes mycorrhizal association in rare lady's slipper orchids, genus Cypripedium.
Identifieur interne : 003629 ( Main/Curation ); précédent : 003628; suivant : 003630High specificity generally characterizes mycorrhizal association in rare lady's slipper orchids, genus Cypripedium.
Auteurs : Richard P. Shefferson [États-Unis] ; Michael Weiss ; Tiiu Kull ; D Lee TaylorSource :
- Molecular ecology [ 0962-1083 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN ribosomique (génétique), Analyse de séquence d'ADN (MeSH), Basidiomycota (génétique), Basidiomycota (physiologie), Données de séquences moléculaires (MeSH), Estonie (MeSH), Fonctions de vraisemblance (MeSH), Modèles génétiques (MeSH), Mycorhizes (génétique), Mycorhizes (physiologie), Orchidaceae (génétique), Orchidaceae (physiologie), Phylogenèse (MeSH), Polymorphisme de restriction (MeSH), Spécificité d'espèce (MeSH), Symbiose (MeSH), Séquence nucléotidique (MeSH), États-Unis (MeSH).
- MESH :
- génétique : ADN ribosomique, Basidiomycota, Mycorhizes, Orchidaceae.
- physiologie : Basidiomycota, Mycorhizes, Orchidaceae.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Estonie, Fonctions de vraisemblance, Modèles génétiques, Phylogenèse, Polymorphisme de restriction, Spécificité d'espèce, Symbiose, Séquence nucléotidique, États-Unis.
- Wicri :
- geographic : Estonie, États-Unis.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Basidiomycota (genetics), Basidiomycota (physiology), DNA, Ribosomal (genetics), Estonia (MeSH), Likelihood Functions (MeSH), Models, Genetic (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (genetics), Mycorrhizae (physiology), Orchidaceae (genetics), Orchidaceae (physiology), Phylogeny (MeSH), Polymorphism, Restriction Fragment Length (MeSH), Sequence Analysis, DNA (MeSH), Species Specificity (MeSH), Symbiosis (MeSH), United States (MeSH).
- MESH :
- chemical , genetics : DNA, Ribosomal.
- geographic : Estonia, United States.
- genetics : Basidiomycota, Mycorrhizae, Orchidaceae.
- physiology : Basidiomycota, Mycorrhizae, Orchidaceae.
- Base Sequence, Likelihood Functions, Models, Genetic, Molecular Sequence Data, Phylogeny, Polymorphism, Restriction Fragment Length, Sequence Analysis, DNA, Species Specificity, Symbiosis.
Abstract
Lady's slipper orchids (Cypripedium spp.) are rare terrestrial plants that grow throughout the temperate Northern Hemisphere. Like all orchids, they require mycorrhizal fungi for germination and seedling nutrition. The nutritional relationships of adult Cypripedium mycorrhizae are unclear; however, Cypripedium distribution may be limited by mycorrhizal specificity, whether this specificity occurs only during the seedling stage or carries on into adulthood. We attempted to identify the primary mycorrhizal symbionts for 100 Cypripedium plants, and successfully did so with two Cypripedium calceolus, 10 Cypripedium californicum, six Cypripedium candidum, 16 Cypripedium fasciculatum, two Cypripedium guttatum, 12 Cypripedium montanum, and 11 Cypripedium parviflorum plants from a total of 44 populations in Europe and North America, yielding fungal nuclear large subunit and mitochondrial large subunit sequence and RFLP (restriction fragment length polymorphism) data for 59 plants. Because orchid mycorrhizal fungi are typically observed without fruiting structures, we assessed fungal identity through direct PCR (polymerase chain reaction) amplification of fungal genes from mycorrhizally colonized root tissue. Phylogenetic analysis revealed that the great majority of Cypripedium mycorrhizal fungi are members of narrow clades within the fungal family Tulasnellaceae. Rarely occurring root endophytes include members of the Sebacinaceae, Ceratobasidiaceae, and the ascomycetous genus, Phialophora. C. californicum was the only orchid species with apparently low specificity, as it associated with tulasnelloid, ceratobasidioid, and sebacinoid fungi in roughly equal proportion. Our results add support to the growing literature showing that high specificity is not limited to nonphotosynthetic plants, but also occurs in photosynthetic ones.
DOI: 10.1111/j.1365-294X.2005.02424.x
PubMed: 15660950
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Shefferson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Integrative Biology, University of California, 3060 Valley Life Sciences Building, #3140, Berkeley, California 94720, USA. dormancy@gmail.com</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Integrative Biology, University of California, 3060 Valley Life Sciences Building, #3140, Berkeley, California 94720</wicri:regionArea></affiliation></author><author><name sortKey="Weiss, Michael" sort="Weiss, Michael" uniqKey="Weiss M" first="Michael" last="Weiss">Michael Weiss</name></author><author><name sortKey="Kull, Tiiu" sort="Kull, Tiiu" uniqKey="Kull T" first="Tiiu" last="Kull">Tiiu Kull</name></author><author><name sortKey="Taylor, D Lee" sort="Taylor, D Lee" uniqKey="Taylor D" first="D Lee" last="Taylor">D Lee Taylor</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15660950</idno><idno type="pmid">15660950</idno><idno type="doi">10.1111/j.1365-294X.2005.02424.x</idno><idno type="wicri:Area/Main/Corpus">003629</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003629</idno><idno type="wicri:Area/Main/Curation">003629</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003629</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">High specificity generally characterizes mycorrhizal association in rare lady's slipper orchids, genus Cypripedium.</title><author><name sortKey="Shefferson, Richard P" sort="Shefferson, Richard P" uniqKey="Shefferson R" first="Richard P" last="Shefferson">Richard P. Shefferson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Integrative Biology, University of California, 3060 Valley Life Sciences Building, #3140, Berkeley, California 94720, USA. dormancy@gmail.com</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Integrative Biology, University of California, 3060 Valley Life Sciences Building, #3140, Berkeley, California 94720</wicri:regionArea></affiliation></author><author><name sortKey="Weiss, Michael" sort="Weiss, Michael" uniqKey="Weiss M" first="Michael" last="Weiss">Michael Weiss</name></author><author><name sortKey="Kull, Tiiu" sort="Kull, Tiiu" uniqKey="Kull T" first="Tiiu" last="Kull">Tiiu Kull</name></author><author><name sortKey="Taylor, D Lee" sort="Taylor, D Lee" uniqKey="Taylor D" first="D Lee" last="Taylor">D Lee Taylor</name></author></analytic><series><title level="j">Molecular ecology</title><idno type="ISSN">0962-1083</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Basidiomycota (genetics)</term><term>Basidiomycota (physiology)</term><term>DNA, Ribosomal (genetics)</term><term>Estonia (MeSH)</term><term>Likelihood Functions (MeSH)</term><term>Models, Genetic (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (physiology)</term><term>Orchidaceae (genetics)</term><term>Orchidaceae (physiology)</term><term>Phylogeny (MeSH)</term><term>Polymorphism, Restriction Fragment Length (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Species Specificity (MeSH)</term><term>Symbiosis (MeSH)</term><term>United States (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ribosomique (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Basidiomycota (génétique)</term><term>Basidiomycota (physiologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Estonie (MeSH)</term><term>Fonctions de vraisemblance (MeSH)</term><term>Modèles génétiques (MeSH)</term><term>Mycorhizes (génétique)</term><term>Mycorhizes (physiologie)</term><term>Orchidaceae (génétique)</term><term>Orchidaceae (physiologie)</term><term>Phylogenèse (MeSH)</term><term>Polymorphisme de restriction (MeSH)</term><term>Spécificité d'espèce (MeSH)</term><term>Symbiose (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>États-Unis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Estonia</term><term>United States</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN ribosomique</term><term>Basidiomycota</term><term>Mycorhizes</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Basidiomycota</term><term>Mycorhizes</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Likelihood Functions</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Polymorphism, Restriction Fragment Length</term><term>Sequence Analysis, DNA</term><term>Species Specificity</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Estonie</term><term>Fonctions de vraisemblance</term><term>Modèles génétiques</term><term>Phylogenèse</term><term>Polymorphisme de restriction</term><term>Spécificité d'espèce</term><term>Symbiose</term><term>Séquence nucléotidique</term><term>États-Unis</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Estonie</term><term>États-Unis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lady's slipper orchids (Cypripedium spp.) are rare terrestrial plants that grow throughout the temperate Northern Hemisphere. Like all orchids, they require mycorrhizal fungi for germination and seedling nutrition. The nutritional relationships of adult Cypripedium mycorrhizae are unclear; however, Cypripedium distribution may be limited by mycorrhizal specificity, whether this specificity occurs only during the seedling stage or carries on into adulthood. We attempted to identify the primary mycorrhizal symbionts for 100 Cypripedium plants, and successfully did so with two Cypripedium calceolus, 10 Cypripedium californicum, six Cypripedium candidum, 16 Cypripedium fasciculatum, two Cypripedium guttatum, 12 Cypripedium montanum, and 11 Cypripedium parviflorum plants from a total of 44 populations in Europe and North America, yielding fungal nuclear large subunit and mitochondrial large subunit sequence and RFLP (restriction fragment length polymorphism) data for 59 plants. Because orchid mycorrhizal fungi are typically observed without fruiting structures, we assessed fungal identity through direct PCR (polymerase chain reaction) amplification of fungal genes from mycorrhizally colonized root tissue. Phylogenetic analysis revealed that the great majority of Cypripedium mycorrhizal fungi are members of narrow clades within the fungal family Tulasnellaceae. Rarely occurring root endophytes include members of the Sebacinaceae, Ceratobasidiaceae, and the ascomycetous genus, Phialophora. C. californicum was the only orchid species with apparently low specificity, as it associated with tulasnelloid, ceratobasidioid, and sebacinoid fungi in roughly equal proportion. Our results add support to the growing literature showing that high specificity is not limited to nonphotosynthetic plants, but also occurs in photosynthetic ones.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15660950</PMID><DateCompleted><Year>2005</Year><Month>04</Month><Day>12</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0962-1083</ISSN><JournalIssue CitedMedium="Print"><Volume>14</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Molecular ecology</Title><ISOAbbreviation>Mol Ecol</ISOAbbreviation></Journal><ArticleTitle>High specificity generally characterizes mycorrhizal association in rare lady's slipper orchids, genus Cypripedium.</ArticleTitle><Pagination><MedlinePgn>613-26</MedlinePgn></Pagination><Abstract><AbstractText>Lady's slipper orchids (Cypripedium spp.) are rare terrestrial plants that grow throughout the temperate Northern Hemisphere. Like all orchids, they require mycorrhizal fungi for germination and seedling nutrition. The nutritional relationships of adult Cypripedium mycorrhizae are unclear; however, Cypripedium distribution may be limited by mycorrhizal specificity, whether this specificity occurs only during the seedling stage or carries on into adulthood. We attempted to identify the primary mycorrhizal symbionts for 100 Cypripedium plants, and successfully did so with two Cypripedium calceolus, 10 Cypripedium californicum, six Cypripedium candidum, 16 Cypripedium fasciculatum, two Cypripedium guttatum, 12 Cypripedium montanum, and 11 Cypripedium parviflorum plants from a total of 44 populations in Europe and North America, yielding fungal nuclear large subunit and mitochondrial large subunit sequence and RFLP (restriction fragment length polymorphism) data for 59 plants. Because orchid mycorrhizal fungi are typically observed without fruiting structures, we assessed fungal identity through direct PCR (polymerase chain reaction) amplification of fungal genes from mycorrhizally colonized root tissue. Phylogenetic analysis revealed that the great majority of Cypripedium mycorrhizal fungi are members of narrow clades within the fungal family Tulasnellaceae. Rarely occurring root endophytes include members of the Sebacinaceae, Ceratobasidiaceae, and the ascomycetous genus, Phialophora. C. californicum was the only orchid species with apparently low specificity, as it associated with tulasnelloid, ceratobasidioid, and sebacinoid fungi in roughly equal proportion. Our results add support to the growing literature showing that high specificity is not limited to nonphotosynthetic plants, but also occurs in photosynthetic ones.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shefferson</LastName><ForeName>Richard P</ForeName><Initials>RP</Initials><AffiliationInfo><Affiliation>Department of Integrative Biology, University of California, 3060 Valley Life Sciences Building, #3140, Berkeley, California 94720, USA. dormancy@gmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Weiss</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Kull</LastName><ForeName>Tiiu</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Taylor</LastName><ForeName>D Lee</ForeName><Initials>DL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Ecol</MedlineTA><NlmUniqueID>9214478</NlmUniqueID><ISSNLinking>0962-1083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004275">DNA, Ribosomal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004275" MajorTopicYN="N">DNA, Ribosomal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004957" MajorTopicYN="N" Type="Geographic">Estonia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016013" MajorTopicYN="N">Likelihood Functions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008957" MajorTopicYN="N">Models, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029595" MajorTopicYN="N">Orchidaceae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012150" MajorTopicYN="N">Polymorphism, Restriction Fragment Length</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014481" MajorTopicYN="N" Type="Geographic">United States</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>1</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>4</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>1</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15660950</ArticleId><ArticleId IdType="pii">MEC2424</ArticleId><ArticleId IdType="doi">10.1111/j.1365-294X.2005.02424.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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