The promoter of the Vicia faba L. leghemoglobin gene VfLb29 is specifically activated in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots from different legume and nonlegume plants.
Identifieur interne : 003805 ( Main/Corpus ); précédent : 003804; suivant : 003806The promoter of the Vicia faba L. leghemoglobin gene VfLb29 is specifically activated in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots from different legume and nonlegume plants.
Auteurs : Martin F. Vieweg ; Martin Frühling ; Hans-Joachim Quandt ; Ute Heim ; Helmut B Umlein ; Alfred Pühler ; Helge Küster ; M Perlick AndreasSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2004.
English descriptors
- KwdEn :
- Anaerobiosis (MeSH), Base Sequence (MeSH), Fabaceae (genetics), Fabaceae (growth & development), Fabaceae (microbiology), Gene Expression Regulation, Fungal (MeSH), Gene Expression Regulation, Plant (MeSH), Leghemoglobin (genetics), Leghemoglobin (metabolism), Medicago (genetics), Medicago (growth & development), Medicago (microbiology), Molecular Sequence Data (MeSH), Mycorrhizae (growth & development), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (genetics), Plant Roots (growth & development), Plant Roots (microbiology), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (genetics), Sequence Homology, Nucleic Acid (MeSH), Symbiosis (genetics), Symbiosis (physiology), Tobacco (genetics), Tobacco (growth & development), Tobacco (microbiology), Vicia faba (genetics), Vicia faba (growth & development), Vicia faba (microbiology).
- MESH :
- chemical , genetics : Leghemoglobin, Plant Proteins.
- genetics : Fabaceae, Medicago, Plant Roots, Promoter Regions, Genetic, Symbiosis, Tobacco, Vicia faba.
- growth & development : Fabaceae, Medicago, Mycorrhizae, Plant Roots, Tobacco, Vicia faba.
- chemical , metabolism : Leghemoglobin, Plant Proteins.
- microbiology : Fabaceae, Medicago, Plant Roots, Tobacco, Vicia faba.
- physiology : Symbiosis.
- Anaerobiosis, Base Sequence, Gene Expression Regulation, Fungal, Gene Expression Regulation, Plant, Molecular Sequence Data, Plants, Genetically Modified, Sequence Homology, Nucleic Acid.
Abstract
The VfLb29 leghemoglobin gene promoter was polymerase chain reaction-amplified from a Vicia faba genomic library and was fused to the gusAint coding region. Expression of the chimeric gene was analyzed in transgenic hairy roots of the legumes V. faba, V. hirsuta, and Medicago truncatula as well as in transgenic Nicotiana tabacum plants. The VfLb29 promoter was found to be specifically active not only in the infected cells of the nitrogen-fixing zone of root nodules but also in arbuscule-containing cells of transgenic V. faba and M. truncatula roots colonized by the endomycorrhizal fungus Glomus intraradices. In addition to these two legumes, specific expression in arbuscule-containing cells was also observed in the nonlegume N. tabacum. All studies were done in comparison to the V. faba leghemoglobin gene promoter VfLb3 that as VfLb29 was expressed in the infected cells of root nodules but showed no activity in endomycorrhiza. An activation of the VfLb29 promoter due to hypoxia in metabolically active tissues was excluded. The conserved activation in arbuscule-containing cells of legumes and the nonlegume N. tabacum suggests a conserved trigger for this promoter in legume and nonlegume endomycorrhiza symbioses.
DOI: 10.1094/MPMI.2004.17.1.62
PubMed: 14714869
Links to Exploration step
pubmed:14714869Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The promoter of the Vicia faba L. leghemoglobin gene VfLb29 is specifically activated in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots from different legume and nonlegume plants.</title><author><name sortKey="Vieweg, Martin F" sort="Vieweg, Martin F" uniqKey="Vieweg M" first="Martin F" last="Vieweg">Martin F. Vieweg</name><affiliation><nlm:affiliation>Universität Bielefeld, Fakultät für Biologie, Lehrstuhl für Genetik, P. O. Box 100131, D-33501 Bielefeld, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Fruhling, Martin" sort="Fruhling, Martin" uniqKey="Fruhling M" first="Martin" last="Frühling">Martin Frühling</name></author><author><name sortKey="Quandt, Hans Joachim" sort="Quandt, Hans Joachim" uniqKey="Quandt H" first="Hans-Joachim" last="Quandt">Hans-Joachim Quandt</name></author><author><name sortKey="Heim, Ute" sort="Heim, Ute" uniqKey="Heim U" first="Ute" last="Heim">Ute Heim</name></author><author><name sortKey="B Umlein, Helmut" sort="B Umlein, Helmut" uniqKey="B Umlein H" first="Helmut" last="B Umlein">Helmut B Umlein</name></author><author><name sortKey="Puhler, Alfred" sort="Puhler, Alfred" uniqKey="Puhler A" first="Alfred" last="Pühler">Alfred Pühler</name></author><author><name sortKey="Kuster, Helge" sort="Kuster, Helge" uniqKey="Kuster H" first="Helge" last="Küster">Helge Küster</name></author><author><name sortKey="Andreas, M Perlick" sort="Andreas, M Perlick" uniqKey="Andreas M" first="M Perlick" last="Andreas">M Perlick Andreas</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:14714869</idno><idno type="pmid">14714869</idno><idno type="doi">10.1094/MPMI.2004.17.1.62</idno><idno type="wicri:Area/Main/Corpus">003805</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003805</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The promoter of the Vicia faba L. leghemoglobin gene VfLb29 is specifically activated in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots from different legume and nonlegume plants.</title><author><name sortKey="Vieweg, Martin F" sort="Vieweg, Martin F" uniqKey="Vieweg M" first="Martin F" last="Vieweg">Martin F. Vieweg</name><affiliation><nlm:affiliation>Universität Bielefeld, Fakultät für Biologie, Lehrstuhl für Genetik, P. O. Box 100131, D-33501 Bielefeld, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Fruhling, Martin" sort="Fruhling, Martin" uniqKey="Fruhling M" first="Martin" last="Frühling">Martin Frühling</name></author><author><name sortKey="Quandt, Hans Joachim" sort="Quandt, Hans Joachim" uniqKey="Quandt H" first="Hans-Joachim" last="Quandt">Hans-Joachim Quandt</name></author><author><name sortKey="Heim, Ute" sort="Heim, Ute" uniqKey="Heim U" first="Ute" last="Heim">Ute Heim</name></author><author><name sortKey="B Umlein, Helmut" sort="B Umlein, Helmut" uniqKey="B Umlein H" first="Helmut" last="B Umlein">Helmut B Umlein</name></author><author><name sortKey="Puhler, Alfred" sort="Puhler, Alfred" uniqKey="Puhler A" first="Alfred" last="Pühler">Alfred Pühler</name></author><author><name sortKey="Kuster, Helge" sort="Kuster, Helge" uniqKey="Kuster H" first="Helge" last="Küster">Helge Küster</name></author><author><name sortKey="Andreas, M Perlick" sort="Andreas, M Perlick" uniqKey="Andreas M" first="M Perlick" last="Andreas">M Perlick Andreas</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anaerobiosis (MeSH)</term><term>Base Sequence (MeSH)</term><term>Fabaceae (genetics)</term><term>Fabaceae (growth & development)</term><term>Fabaceae (microbiology)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Leghemoglobin (genetics)</term><term>Leghemoglobin (metabolism)</term><term>Medicago (genetics)</term><term>Medicago (growth & development)</term><term>Medicago (microbiology)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (growth & development)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (genetics)</term><term>Plant Roots (growth & development)</term><term>Plant Roots (microbiology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (genetics)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term><term>Symbiosis (genetics)</term><term>Symbiosis (physiology)</term><term>Tobacco (genetics)</term><term>Tobacco (growth & development)</term><term>Tobacco (microbiology)</term><term>Vicia faba (genetics)</term><term>Vicia faba (growth & development)</term><term>Vicia faba (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Leghemoglobin</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fabaceae</term><term>Medicago</term><term>Plant Roots</term><term>Promoter Regions, Genetic</term><term>Symbiosis</term><term>Tobacco</term><term>Vicia faba</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fabaceae</term><term>Medicago</term><term>Mycorrhizae</term><term>Plant Roots</term><term>Tobacco</term><term>Vicia faba</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Leghemoglobin</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Fabaceae</term><term>Medicago</term><term>Plant Roots</term><term>Tobacco</term><term>Vicia faba</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Anaerobiosis</term><term>Base Sequence</term><term>Gene Expression Regulation, Fungal</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Plants, Genetically Modified</term><term>Sequence Homology, Nucleic Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The VfLb29 leghemoglobin gene promoter was polymerase chain reaction-amplified from a Vicia faba genomic library and was fused to the gusAint coding region. Expression of the chimeric gene was analyzed in transgenic hairy roots of the legumes V. faba, V. hirsuta, and Medicago truncatula as well as in transgenic Nicotiana tabacum plants. The VfLb29 promoter was found to be specifically active not only in the infected cells of the nitrogen-fixing zone of root nodules but also in arbuscule-containing cells of transgenic V. faba and M. truncatula roots colonized by the endomycorrhizal fungus Glomus intraradices. In addition to these two legumes, specific expression in arbuscule-containing cells was also observed in the nonlegume N. tabacum. All studies were done in comparison to the V. faba leghemoglobin gene promoter VfLb3 that as VfLb29 was expressed in the infected cells of root nodules but showed no activity in endomycorrhiza. An activation of the VfLb29 promoter due to hypoxia in metabolically active tissues was excluded. The conserved activation in arbuscule-containing cells of legumes and the nonlegume N. tabacum suggests a conserved trigger for this promoter in legume and nonlegume endomycorrhiza symbioses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14714869</PMID><DateCompleted><Year>2004</Year><Month>04</Month><Day>02</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>The promoter of the Vicia faba L. leghemoglobin gene VfLb29 is specifically activated in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots from different legume and nonlegume plants.</ArticleTitle><Pagination><MedlinePgn>62-9</MedlinePgn></Pagination><Abstract><AbstractText>The VfLb29 leghemoglobin gene promoter was polymerase chain reaction-amplified from a Vicia faba genomic library and was fused to the gusAint coding region. Expression of the chimeric gene was analyzed in transgenic hairy roots of the legumes V. faba, V. hirsuta, and Medicago truncatula as well as in transgenic Nicotiana tabacum plants. The VfLb29 promoter was found to be specifically active not only in the infected cells of the nitrogen-fixing zone of root nodules but also in arbuscule-containing cells of transgenic V. faba and M. truncatula roots colonized by the endomycorrhizal fungus Glomus intraradices. In addition to these two legumes, specific expression in arbuscule-containing cells was also observed in the nonlegume N. tabacum. All studies were done in comparison to the V. faba leghemoglobin gene promoter VfLb3 that as VfLb29 was expressed in the infected cells of root nodules but showed no activity in endomycorrhiza. An activation of the VfLb29 promoter due to hypoxia in metabolically active tissues was excluded. The conserved activation in arbuscule-containing cells of legumes and the nonlegume N. tabacum suggests a conserved trigger for this promoter in legume and nonlegume endomycorrhiza symbioses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vieweg</LastName><ForeName>Martin F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Universität Bielefeld, Fakultät für Biologie, Lehrstuhl für Genetik, P. O. Box 100131, D-33501 Bielefeld, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Frühling</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Quandt</LastName><ForeName>Hans-Joachim</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>Heim</LastName><ForeName>Ute</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Bäumlein</LastName><ForeName>Helmut</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Pühler</LastName><ForeName>Alfred</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Küster</LastName><ForeName>Helge</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Andreas</LastName><ForeName>M Perlick</ForeName><Initials>MP</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AJ564166</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007874">Leghemoglobin</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000693" MajorTopicYN="N">Anaerobiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007887" MajorTopicYN="N">Fabaceae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007874" MajorTopicYN="N">Leghemoglobin</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029902" MajorTopicYN="N">Medicago</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031307" MajorTopicYN="N">Vicia faba</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>1</Month><Day>13</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>4</Month><Day>3</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>1</Month><Day>13</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14714869</ArticleId><ArticleId IdType="doi">10.1094/MPMI.2004.17.1.62</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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