Activity profiling of ectomycorrhiza communities in two forest soils using multiple enzymatic tests.
Identifieur interne : 003526 ( Main/Corpus ); précédent : 003525; suivant : 003527Activity profiling of ectomycorrhiza communities in two forest soils using multiple enzymatic tests.
Auteurs : Pierre-Emmanuel Courty ; Karin Pritsch ; Michael Schloter ; Anton Hartmann ; Jean GarbayeSource :
- The New phytologist [ 0028-646X ] ; 2005.
English descriptors
- KwdEn :
- Biodiversity (MeSH), Cellulose 1,4-beta-Cellobiosidase (metabolism), Fungal Proteins (metabolism), Glucuronidase (metabolism), Hydrogen-Ion Concentration (MeSH), Laccase (metabolism), Leucyl Aminopeptidase (metabolism), Mycorrhizae (enzymology), Photometry (methods), Plant Roots (microbiology), Soil Microbiology (MeSH), Trees (microbiology), Xylosidases (metabolism).
- MESH :
- chemical , metabolism : Cellulose 1,4-beta-Cellobiosidase, Fungal Proteins, Glucuronidase, Laccase, Leucyl Aminopeptidase, Xylosidases.
- enzymology : Mycorrhizae.
- methods : Photometry.
- microbiology : Plant Roots, Trees.
- Biodiversity, Hydrogen-Ion Concentration, Soil Microbiology.
Abstract
Data on the diversity and distribution of enzyme activities in native ectomycorrhizal (ECM) communities are inadequate. A microplate multiple enzymatic test was developed which makes it possible to measure eight enzyme activities on 14 individual, excised ECM root tips. Hydrolytic and oxidative enzymes are involved in the decomposition of lignocellulose, chitin and phosphorus-containing organic compounds. This test system was used to describe the functional diversity of ECM communities in two forest sites. This set of tests proved to be accurate and sensitive enough to reveal a high diversity of activity profiles, depending on the fungal symbiont and the soil horizon. Ectomycorrhizas can be classified into specialists and generalists, and appear to complement each other in the same horizon to collectively perform all eight activities studied. By including a higher number of different assays for more detailed analyses, ECM activity profiling will provide a valuable tool for studying the functional diversity of ECM communities.
DOI: 10.1111/j.1469-8137.2005.01401.x
PubMed: 15948852
Links to Exploration step
pubmed:15948852Le document en format XML
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A microplate multiple enzymatic test was developed which makes it possible to measure eight enzyme activities on 14 individual, excised ECM root tips. Hydrolytic and oxidative enzymes are involved in the decomposition of lignocellulose, chitin and phosphorus-containing organic compounds. This test system was used to describe the functional diversity of ECM communities in two forest sites. This set of tests proved to be accurate and sensitive enough to reveal a high diversity of activity profiles, depending on the fungal symbiont and the soil horizon. Ectomycorrhizas can be classified into specialists and generalists, and appear to complement each other in the same horizon to collectively perform all eight activities studied. By including a higher number of different assays for more detailed analyses, ECM activity profiling will provide a valuable tool for studying the functional diversity of ECM communities.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15948852</PMID><DateCompleted><Year>2005</Year><Month>08</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0028-646X</ISSN><JournalIssue CitedMedium="Print"><Volume>167</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jul</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Activity profiling of ectomycorrhiza communities in two forest soils using multiple enzymatic tests.</ArticleTitle><Pagination><MedlinePgn>309-19</MedlinePgn></Pagination><Abstract><AbstractText>Data on the diversity and distribution of enzyme activities in native ectomycorrhizal (ECM) communities are inadequate. A microplate multiple enzymatic test was developed which makes it possible to measure eight enzyme activities on 14 individual, excised ECM root tips. Hydrolytic and oxidative enzymes are involved in the decomposition of lignocellulose, chitin and phosphorus-containing organic compounds. This test system was used to describe the functional diversity of ECM communities in two forest sites. This set of tests proved to be accurate and sensitive enough to reveal a high diversity of activity profiles, depending on the fungal symbiont and the soil horizon. Ectomycorrhizas can be classified into specialists and generalists, and appear to complement each other in the same horizon to collectively perform all eight activities studied. By including a higher number of different assays for more detailed analyses, ECM activity profiling will provide a valuable tool for studying the functional diversity of ECM communities.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Courty</LastName><ForeName>Pierre-Emmanuel</ForeName><Initials>PE</Initials><AffiliationInfo><Affiliation>Unité Mixte de Recherche INRA-UHP 1136 'Interactions Arbres/Microorganismes', F-54280 Champenoux, France. courty@nancy.inra.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pritsch</LastName><ForeName>Karin</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Schloter</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Hartmann</LastName><ForeName>Anton</ForeName><Initials>A</Initials></Author><Author 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MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014995" MajorTopicYN="N">Xylosidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>6</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>8</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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