Combined transcriptome profiling reveals a novel family of arbuscular mycorrhizal-specific Medicago truncatula lectin genes.
Identifieur interne : 003465 ( Main/Corpus ); précédent : 003464; suivant : 003466Combined transcriptome profiling reveals a novel family of arbuscular mycorrhizal-specific Medicago truncatula lectin genes.
Auteurs : André Frenzel ; Katja Manthey ; Andreas M. Perlick ; Folker Meyer ; Alfred Pühler ; Helge Küster ; Franziska KrajinskiSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2005.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cluster Analysis (MeSH), Expressed Sequence Tags (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (genetics), Genes, Plant (genetics), Lectins (chemistry), Lectins (genetics), Lectins (metabolism), Medicago truncatula (genetics), Medicago truncatula (metabolism), Mycorrhizae (metabolism), Phylogeny (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Symbiosis (MeSH), Transcription, Genetic (genetics).
- MESH :
- chemical , chemistry : Lectins.
- genetics : Gene Expression Regulation, Plant, Genes, Plant, Lectins, Medicago truncatula, Plant Proteins, Transcription, Genetic.
- chemical , metabolism : Lectins, Medicago truncatula, Mycorrhizae, Plant Proteins.
- Amino Acid Sequence, Cluster Analysis, Expressed Sequence Tags, Gene Expression Profiling, Phylogeny, Symbiosis.
Abstract
The large majority of plants are capable of undergoing a tight symbiosis with arbuscular mycorrhizal (AM) fungi. During this symbiosis, highly specialized new structures called arbuscules are formed within the host cells, indicating that, during interaction with AM fungi, plants express AM-specific genetic programs. Despite increasing efforts, the number of genes known to be induced in the AM symbiosis is still low. In order to identify novel AM-induced genes which have not been listed before, 5,646 expressed sequence tags (ESTs) were generated from two Medicago truncatula cDNA libraries: a random cDNA library (MtAmp) and a suppression subtractive hybridization (SSH) library (MtGim), the latter being designed to enhance the cloning of mycorrhiza-upregulated genes. In silico expression analysis was applied to identify those tentative consensus sequences (TCs) of The Institute for Genomic Research M. truncatula gene index (MtGI) that are composed exclusively of ESTs deriving from the MtGim or MtAmp library, but not from any other cDNA library of the MtGI. This search revealed 115 MtAmp- or MTGim-specific TCs. For the majority of these TCs with sequence similarities to plant genes, the AM-specific expression was verified by quantitative reverse-transcription polymerase chain reaction. Annotation of the novel genes induced in mycorrhizal roots suggested their involvement in different transport as well as signaling processes and revealed a novel family of AM-specific lectin genes. The expression of reporter gene fusions in transgenic roots revealed an arbuscule-related expression of two members of the lectin gene family, indicating a role for AM-specific lectins during arbuscule formation or functioning.
DOI: 10.1094/MPMI-18-0771
PubMed: 16134889
Links to Exploration step
pubmed:16134889Le document en format XML
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<author><name sortKey="Frenzel, Andre" sort="Frenzel, Andre" uniqKey="Frenzel A" first="André" last="Frenzel">André Frenzel</name>
<affiliation><nlm:affiliation>Lehrgebiet Molekulargenetik, Universität Hannover, Herrenhäuser Str. 2, D-30419 Hannover, Germany.</nlm:affiliation>
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<author><name sortKey="Manthey, Katja" sort="Manthey, Katja" uniqKey="Manthey K" first="Katja" last="Manthey">Katja Manthey</name>
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<author><name sortKey="Perlick, Andreas M" sort="Perlick, Andreas M" uniqKey="Perlick A" first="Andreas M" last="Perlick">Andreas M. Perlick</name>
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<author><name sortKey="Meyer, Folker" sort="Meyer, Folker" uniqKey="Meyer F" first="Folker" last="Meyer">Folker Meyer</name>
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<author><name sortKey="Puhler, Alfred" sort="Puhler, Alfred" uniqKey="Puhler A" first="Alfred" last="Pühler">Alfred Pühler</name>
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<author><name sortKey="Kuster, Helge" sort="Kuster, Helge" uniqKey="Kuster H" first="Helge" last="Küster">Helge Küster</name>
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<author><name sortKey="Krajinski, Franziska" sort="Krajinski, Franziska" uniqKey="Krajinski F" first="Franziska" last="Krajinski">Franziska Krajinski</name>
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<term>Cluster Analysis (MeSH)</term>
<term>Expressed Sequence Tags (MeSH)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Plant (genetics)</term>
<term>Genes, Plant (genetics)</term>
<term>Lectins (chemistry)</term>
<term>Lectins (genetics)</term>
<term>Lectins (metabolism)</term>
<term>Medicago truncatula (genetics)</term>
<term>Medicago truncatula (metabolism)</term>
<term>Mycorrhizae (metabolism)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Symbiosis (MeSH)</term>
<term>Transcription, Genetic (genetics)</term>
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<term>Genes, Plant</term>
<term>Lectins</term>
<term>Medicago truncatula</term>
<term>Plant Proteins</term>
<term>Transcription, Genetic</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lectins</term>
<term>Medicago truncatula</term>
<term>Mycorrhizae</term>
<term>Plant Proteins</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Cluster Analysis</term>
<term>Expressed Sequence Tags</term>
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<front><div type="abstract" xml:lang="en">The large majority of plants are capable of undergoing a tight symbiosis with arbuscular mycorrhizal (AM) fungi. During this symbiosis, highly specialized new structures called arbuscules are formed within the host cells, indicating that, during interaction with AM fungi, plants express AM-specific genetic programs. Despite increasing efforts, the number of genes known to be induced in the AM symbiosis is still low. In order to identify novel AM-induced genes which have not been listed before, 5,646 expressed sequence tags (ESTs) were generated from two Medicago truncatula cDNA libraries: a random cDNA library (MtAmp) and a suppression subtractive hybridization (SSH) library (MtGim), the latter being designed to enhance the cloning of mycorrhiza-upregulated genes. In silico expression analysis was applied to identify those tentative consensus sequences (TCs) of The Institute for Genomic Research M. truncatula gene index (MtGI) that are composed exclusively of ESTs deriving from the MtGim or MtAmp library, but not from any other cDNA library of the MtGI. This search revealed 115 MtAmp- or MTGim-specific TCs. For the majority of these TCs with sequence similarities to plant genes, the AM-specific expression was verified by quantitative reverse-transcription polymerase chain reaction. Annotation of the novel genes induced in mycorrhizal roots suggested their involvement in different transport as well as signaling processes and revealed a novel family of AM-specific lectin genes. The expression of reporter gene fusions in transgenic roots revealed an arbuscule-related expression of two members of the lectin gene family, indicating a role for AM-specific lectins during arbuscule formation or functioning.</div>
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<Abstract><AbstractText>The large majority of plants are capable of undergoing a tight symbiosis with arbuscular mycorrhizal (AM) fungi. During this symbiosis, highly specialized new structures called arbuscules are formed within the host cells, indicating that, during interaction with AM fungi, plants express AM-specific genetic programs. Despite increasing efforts, the number of genes known to be induced in the AM symbiosis is still low. In order to identify novel AM-induced genes which have not been listed before, 5,646 expressed sequence tags (ESTs) were generated from two Medicago truncatula cDNA libraries: a random cDNA library (MtAmp) and a suppression subtractive hybridization (SSH) library (MtGim), the latter being designed to enhance the cloning of mycorrhiza-upregulated genes. In silico expression analysis was applied to identify those tentative consensus sequences (TCs) of The Institute for Genomic Research M. truncatula gene index (MtGI) that are composed exclusively of ESTs deriving from the MtGim or MtAmp library, but not from any other cDNA library of the MtGI. This search revealed 115 MtAmp- or MTGim-specific TCs. For the majority of these TCs with sequence similarities to plant genes, the AM-specific expression was verified by quantitative reverse-transcription polymerase chain reaction. Annotation of the novel genes induced in mycorrhizal roots suggested their involvement in different transport as well as signaling processes and revealed a novel family of AM-specific lectin genes. The expression of reporter gene fusions in transgenic roots revealed an arbuscule-related expression of two members of the lectin gene family, indicating a role for AM-specific lectins during arbuscule formation or functioning.</AbstractText>
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