Influence of long-term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi.
Identifieur interne : 003177 ( Main/Corpus ); précédent : 003176; suivant : 003178Influence of long-term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi.
Auteurs : Brigitte A. Bastias ; Zhihong Xu ; John W G. CairneySource :
- The New phytologist [ 0028-646X ] ; 2006.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Fungal, DNA, Ribosomal.
- geographic : Australia.
- physiology : Mycorrhizae.
- Ecosystem, Fires, Soil Microbiology.
Abstract
To demonstrate the efficacy of direct DNA extraction from hyphal ingrowth bags for community profiling of ectomycorrhizal (ECM) mycelia in soil, we applied the method to investigate the influence of long-term repeated prescribed burning on an ECM fungal community. DNA was extracted from hyphal ingrowth bags buried in forest plots that received different prescribed burning treatments for 30 yr, and denaturing gradient gel electrophoresis (DGGE) profiles of partial fungal rDNA internal transcribed spacer (ITS) regions were compared. Restriction fragment length polymorphism (RFLP) and sequence analyses were also used to compare clone assemblages between the treatments. The majority of sequences derived from the ingrowth bags were apparently those of ECM fungi. DGGE profiles for biennially burned plots were significantly different from those of quadrennially burned and unburned control plots. Analysis of clone assemblages indicated that this reflected altered ECM fungal community composition. The results indicate that hyphal ingrowth bags represent a useful method for investigation of ECM mycelial communities, and that frequent long-term prescribed burning can influence below-ground ECM fungal communities.
DOI: 10.1111/j.1469-8137.2006.01793.x
PubMed: 16945097
Links to Exploration step
pubmed:16945097Le document en format XML
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<author><name sortKey="Bastias, Brigitte A" sort="Bastias, Brigitte A" uniqKey="Bastias B" first="Brigitte A" last="Bastias">Brigitte A. Bastias</name>
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<author><name sortKey="Xu, Zhihong" sort="Xu, Zhihong" uniqKey="Xu Z" first="Zhihong" last="Xu">Zhihong Xu</name>
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<author><name sortKey="Cairney, John W G" sort="Cairney, John W G" uniqKey="Cairney J" first="John W G" last="Cairney">John W G. Cairney</name>
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<series><title level="j">The New phytologist</title>
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<term>Fires (MeSH)</term>
<term>Mycorrhizae (physiology)</term>
<term>Soil Microbiology (MeSH)</term>
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<front><div type="abstract" xml:lang="en">To demonstrate the efficacy of direct DNA extraction from hyphal ingrowth bags for community profiling of ectomycorrhizal (ECM) mycelia in soil, we applied the method to investigate the influence of long-term repeated prescribed burning on an ECM fungal community. DNA was extracted from hyphal ingrowth bags buried in forest plots that received different prescribed burning treatments for 30 yr, and denaturing gradient gel electrophoresis (DGGE) profiles of partial fungal rDNA internal transcribed spacer (ITS) regions were compared. Restriction fragment length polymorphism (RFLP) and sequence analyses were also used to compare clone assemblages between the treatments. The majority of sequences derived from the ingrowth bags were apparently those of ECM fungi. DGGE profiles for biennially burned plots were significantly different from those of quadrennially burned and unburned control plots. Analysis of clone assemblages indicated that this reflected altered ECM fungal community composition. The results indicate that hyphal ingrowth bags represent a useful method for investigation of ECM mycelial communities, and that frequent long-term prescribed burning can influence below-ground ECM fungal communities.</div>
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<Abstract><AbstractText>To demonstrate the efficacy of direct DNA extraction from hyphal ingrowth bags for community profiling of ectomycorrhizal (ECM) mycelia in soil, we applied the method to investigate the influence of long-term repeated prescribed burning on an ECM fungal community. DNA was extracted from hyphal ingrowth bags buried in forest plots that received different prescribed burning treatments for 30 yr, and denaturing gradient gel electrophoresis (DGGE) profiles of partial fungal rDNA internal transcribed spacer (ITS) regions were compared. Restriction fragment length polymorphism (RFLP) and sequence analyses were also used to compare clone assemblages between the treatments. The majority of sequences derived from the ingrowth bags were apparently those of ECM fungi. DGGE profiles for biennially burned plots were significantly different from those of quadrennially burned and unburned control plots. Analysis of clone assemblages indicated that this reflected altered ECM fungal community composition. The results indicate that hyphal ingrowth bags represent a useful method for investigation of ECM mycelial communities, and that frequent long-term prescribed burning can influence below-ground ECM fungal communities.</AbstractText>
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