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Molecular analysis of arbuscular mycorrhizal fungi colonising a semi-natural grassland along a fertilisation gradient.

Identifieur interne : 003176 ( Main/Corpus ); précédent : 003175; suivant : 003177

Molecular analysis of arbuscular mycorrhizal fungi colonising a semi-natural grassland along a fertilisation gradient.

Auteurs : Juan C. Santos ; Roger D. Finlay ; Anders Tehler

Source :

RBID : pubmed:16945098

English descriptors

Abstract

The community of arbuscular mycorrhizal fungi (AMF) colonizing the roots of Festuca pratensis and Achillea millefolium was characterized in a Swedish pasture at different times, along a gradient of fertilization. The small subunit ribosomal RNA gene was subjected to PCR and denaturing gradient gel electrophoresis (DGGE), sequencing and phylogenetic analysis. The sequences found in this study clustered in 10 discrete sequence groups, seven belonging to Glomus, two to Scutellospora and one to Diversispora. A negative correlation was observed between soil mineral nitrogen and the number of AMF sequence groups in the roots. The frequency of occurrence of AMF in roots decreased dramatically between June and September. No plant-host specificity could be detected.

DOI: 10.1111/j.1469-8137.2006.01799.x
PubMed: 16945098

Links to Exploration step

pubmed:16945098

Le document en format XML

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<nlm:affiliation>Department of Cryptogamic Botany, Swedish Museum of Natural History, PO Box 50007, SE-104 05 Stockholm, Sweden. juan.santos@nrm.se</nlm:affiliation>
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<name sortKey="Finlay, Roger D" sort="Finlay, Roger D" uniqKey="Finlay R" first="Roger D" last="Finlay">Roger D. Finlay</name>
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<term>Achillea (microbiology)</term>
<term>Fertilizers (MeSH)</term>
<term>Festuca (microbiology)</term>
<term>Mycorrhizae (genetics)</term>
<term>Nitrogen (chemistry)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Roots (microbiology)</term>
<term>RNA, Fungal (genetics)</term>
<term>RNA, Ribosomal (genetics)</term>
<term>Soil (analysis)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en">
<term>Soil</term>
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<term>Mycorrhizae</term>
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<div type="abstract" xml:lang="en">The community of arbuscular mycorrhizal fungi (AMF) colonizing the roots of Festuca pratensis and Achillea millefolium was characterized in a Swedish pasture at different times, along a gradient of fertilization. The small subunit ribosomal RNA gene was subjected to PCR and denaturing gradient gel electrophoresis (DGGE), sequencing and phylogenetic analysis. The sequences found in this study clustered in 10 discrete sequence groups, seven belonging to Glomus, two to Scutellospora and one to Diversispora. A negative correlation was observed between soil mineral nitrogen and the number of AMF sequence groups in the roots. The frequency of occurrence of AMF in roots decreased dramatically between June and September. No plant-host specificity could be detected.</div>
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