GintMT1 encodes a functional metallothionein in Glomus intraradices that responds to oxidative stress.
Identifieur interne : 003035 ( Main/Corpus ); précédent : 003034; suivant : 003036GintMT1 encodes a functional metallothionein in Glomus intraradices that responds to oxidative stress.
Auteurs : M. González-Guerrero ; C. Cano ; C. Azc N-Aguilar ; N. FerrolSource :
- Mycorrhiza [ 0940-6360 ] ; 2007.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Daucus carota (MeSH), Gene Expression Regulation, Fungal (MeSH), Genes, Fungal (genetics), Metalloproteins (genetics), Metalloproteins (physiology), Molecular Sequence Data (MeSH), Mycorrhizae (physiology), Oxidation-Reduction (MeSH), Oxidative Stress (MeSH), Sequence Alignment (MeSH).
- MESH :
- chemical , genetics : Metalloproteins.
- genetics : Genes, Fungal.
- chemical , physiology : Metalloproteins, Mycorrhizae.
- Amino Acid Sequence, Daucus carota, Gene Expression Regulation, Fungal, Molecular Sequence Data, Oxidation-Reduction, Oxidative Stress, Sequence Alignment.
Abstract
A full-length metallothionein (MT) gene (GintMT1) was isolated from Glomus intraradices extraradical mycelium. This is the first MT gene reported in the genus Glomus, third in the Glomeromycota. Functional analysis of GintMT1 in a MT-defective Saccharomyces cerevisiae strain indicates that it encodes a functional MT. Gene expression analyses revealed that the transcript levels of GintMT1 were elevated in mycelia treated with 5 mM Cu or paraquat but inhibited in mycelia treated with 50 microM Cu or 450 microM Cd. The elevated expression of GintMT1 in the 5 mM Cu-treated mycelia together with the ability of GintMT1 to provide tolerance to a Cu-sensitive yeast suggests that GintMT1 might afford protection against Cu. Induction of GintMT1 expression by paraquat and 5 mM Cu, treatments that also produced an oxidative damage to the fungal membranes, suggests that GintMT1 may play a role in the regulation of the redox status of the extraradical mycelium of G. intraradices.
DOI: 10.1007/s00572-007-0108-7
PubMed: 17277942
Links to Exploration step
pubmed:17277942Le document en format XML
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Profesor Albareda 1, 18008, Granada, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Azc N Aguilar, C" sort="Azc N Aguilar, C" uniqKey="Azc N Aguilar C" first="C" last="Azc N-Aguilar">C. Azc N-Aguilar</name><affiliation><nlm:affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Ferrol, N" sort="Ferrol, N" uniqKey="Ferrol N" first="N" last="Ferrol">N. Ferrol</name><affiliation><nlm:affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain. nferrol@eez.csic.es.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17277942</idno><idno type="pmid">17277942</idno><idno type="doi">10.1007/s00572-007-0108-7</idno><idno type="wicri:Area/Main/Corpus">003035</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003035</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">GintMT1 encodes a functional metallothionein in Glomus intraradices that responds to oxidative stress.</title><author><name sortKey="Gonzalez Guerrero, M" sort="Gonzalez Guerrero, M" uniqKey="Gonzalez Guerrero M" first="M" last="González-Guerrero">M. González-Guerrero</name><affiliation><nlm:affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Cano, C" sort="Cano, C" uniqKey="Cano C" first="C" last="Cano">C. Cano</name><affiliation><nlm:affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Azc N Aguilar, C" sort="Azc N Aguilar, C" uniqKey="Azc N Aguilar C" first="C" last="Azc N-Aguilar">C. Azc N-Aguilar</name><affiliation><nlm:affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Ferrol, N" sort="Ferrol, N" uniqKey="Ferrol N" first="N" last="Ferrol">N. Ferrol</name><affiliation><nlm:affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain. nferrol@eez.csic.es.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="ISSN">0940-6360</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Daucus carota (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Genes, Fungal (genetics)</term><term>Metalloproteins (genetics)</term><term>Metalloproteins (physiology)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Oxidation-Reduction (MeSH)</term><term>Oxidative Stress (MeSH)</term><term>Sequence Alignment (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Metalloproteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Fungal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Metalloproteins</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Daucus carota</term><term>Gene Expression Regulation, Fungal</term><term>Molecular Sequence Data</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term><term>Sequence Alignment</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A full-length metallothionein (MT) gene (GintMT1) was isolated from Glomus intraradices extraradical mycelium. This is the first MT gene reported in the genus Glomus, third in the Glomeromycota. Functional analysis of GintMT1 in a MT-defective Saccharomyces cerevisiae strain indicates that it encodes a functional MT. Gene expression analyses revealed that the transcript levels of GintMT1 were elevated in mycelia treated with 5 mM Cu or paraquat but inhibited in mycelia treated with 50 microM Cu or 450 microM Cd. The elevated expression of GintMT1 in the 5 mM Cu-treated mycelia together with the ability of GintMT1 to provide tolerance to a Cu-sensitive yeast suggests that GintMT1 might afford protection against Cu. Induction of GintMT1 expression by paraquat and 5 mM Cu, treatments that also produced an oxidative damage to the fungal membranes, suggests that GintMT1 may play a role in the regulation of the redox status of the extraradical mycelium of G. intraradices.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17277942</PMID><DateCompleted><Year>2007</Year><Month>11</Month><Day>13</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0940-6360</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>4</Issue><PubDate><Year>2007</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>GintMT1 encodes a functional metallothionein in Glomus intraradices that responds to oxidative stress.</ArticleTitle><Pagination><MedlinePgn>327-335</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-007-0108-7</ELocationID><Abstract><AbstractText>A full-length metallothionein (MT) gene (GintMT1) was isolated from Glomus intraradices extraradical mycelium. This is the first MT gene reported in the genus Glomus, third in the Glomeromycota. Functional analysis of GintMT1 in a MT-defective Saccharomyces cerevisiae strain indicates that it encodes a functional MT. Gene expression analyses revealed that the transcript levels of GintMT1 were elevated in mycelia treated with 5 mM Cu or paraquat but inhibited in mycelia treated with 50 microM Cu or 450 microM Cd. The elevated expression of GintMT1 in the 5 mM Cu-treated mycelia together with the ability of GintMT1 to provide tolerance to a Cu-sensitive yeast suggests that GintMT1 might afford protection against Cu. Induction of GintMT1 expression by paraquat and 5 mM Cu, treatments that also produced an oxidative damage to the fungal membranes, suggests that GintMT1 may play a role in the regulation of the redox status of the extraradical mycelium of G. intraradices.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>González-Guerrero</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cano</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Azcón-Aguilar</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ferrol</LastName><ForeName>N</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C. Profesor Albareda 1, 18008, Granada, Spain. nferrol@eez.csic.es.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>02</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008667">Metalloproteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018552" MajorTopicYN="N">Daucus carota</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008667" MajorTopicYN="N">Metalloproteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName 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