Genetic diversity and differential in vitro responses to Ni in Cenococcum geophilum isolates from serpentine soils in Portugal.
Identifieur interne : 002E59 ( Main/Corpus ); précédent : 002E58; suivant : 002E60Genetic diversity and differential in vitro responses to Ni in Cenococcum geophilum isolates from serpentine soils in Portugal.
Auteurs : Susana C. Gonçalves ; Ant Nio Portugal ; M Teresa Gonçalves ; Rita Vieira ; M Amélia Martins-Loução ; Helena FreitasSource :
- Mycorrhiza [ 0940-6360 ] ; 2007.
English descriptors
- KwdEn :
- Amplified Fragment Length Polymorphism Analysis (MeSH), Ascomycota (drug effects), Ascomycota (genetics), Ascomycota (isolation & purification), Ascomycota (metabolism), DNA, Fungal (chemistry), DNA, Fungal (genetics), Genetic Variation (MeSH), Mycorrhizae (drug effects), Mycorrhizae (genetics), Mycorrhizae (isolation & purification), Mycorrhizae (metabolism), Nickel (pharmacology), Portugal (MeSH), Quercus (microbiology), Soil Microbiology (MeSH), Trees (microbiology).
- MESH :
- chemical , chemistry : DNA, Fungal.
- geographic : Portugal.
- drug effects : Ascomycota, Mycorrhizae.
- genetics : Ascomycota, DNA, Fungal, Mycorrhizae.
- isolation & purification : Ascomycota, Mycorrhizae.
- metabolism : Ascomycota, Mycorrhizae.
- microbiology : Quercus, Trees.
- chemical , pharmacology : Nickel.
- Amplified Fragment Length Polymorphism Analysis, Genetic Variation, Soil Microbiology.
Abstract
Amplified fragment length polymorphism (AFLP) analysis was used to investigate the genetic diversity in isolates of the ectomycorrhizal fungus Cenococcum geophilum from serpentine and non-serpentine soils in Portugal. A high degree of genetic diversity was found among C. geophilum isolates; AFLP fingerprints showed that all the isolates were genetically distinct. We also assessed the in vitro Ni sensitivity in three serpentine isolates and one non-serpentine isolate. Only the non-serpentine isolate was significantly affected by the addition of Ni to the growth medium. At 30 microg g(-1) Ni, radial growth rate and biomass accumulation decreased to 73.3 and 71.6% of control, respectively, a highly significant inhibitory effect. Nickel at this concentration had no significant inhibitory effect on serpentine isolates, and so the fitness of serpentine isolates, as evaluated by radial growth rate and biomass yield, is likely unaffected by Ni in the field. In all isolates, the Ni concentration in the mycelia increased with increasing Ni concentration in the growth medium, but two profiles of Ni accumulation were identified. One serpentine isolate showed a linear trend of Ni accumulation. At the highest Ni exposure, the concentration of Ni in the mycelium of this isolate was in the hyperaccumulation range for Ni as defined for higher plants. In the remaining isolates, Ni accumulation was less pronounced and seems to approach a plateau at 30 microg g(-1) Ni. Because two profiles of Ni accumulation emerged among our Ni-insensitive serpentine isolates, this result suggests that different Ni detoxification pathways may be operating. The non-serpentine isolate whose growth was significantly affected by Ni was separated from the other isolates in the genetic analysis, suggesting a genetic basis for the Ni-sensitivity trait. This hypothesis is further supported by the fact that all isolates were maintained on medium without added Ni to avoid carry-over effects. However, because AFLP analysis failed to distinguish between serpentine and non-serpentine isolates, we cannot conclude that Ni insensitivity among our serpentine isolates is due to evolutionary adaptation. Screening a larger number of isolates, from different geographical origins and environments, should clarify the relationships between genetic diversity, morphology, and physiology in this important species.
DOI: 10.1007/s00572-007-0145-2
PubMed: 17710447
Links to Exploration step
pubmed:17710447Le document en format XML
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Gonçalves</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal. scgoncal@ci.uc.pt.</nlm:affiliation></affiliation></author><author><name sortKey="Portugal, Ant Nio" sort="Portugal, Ant Nio" uniqKey="Portugal A" first="Ant Nio" last="Portugal">Ant Nio Portugal</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</nlm:affiliation></affiliation></author><author><name sortKey="Goncalves, M Teresa" sort="Goncalves, M Teresa" uniqKey="Goncalves M" first="M Teresa" last="Gonçalves">M Teresa Gonçalves</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</nlm:affiliation></affiliation></author><author><name sortKey="Vieira, Rita" sort="Vieira, Rita" uniqKey="Vieira R" first="Rita" last="Vieira">Rita Vieira</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</nlm:affiliation></affiliation></author><author><name sortKey="Martins Loucao, M Amelia" sort="Martins Loucao, M Amelia" uniqKey="Martins Loucao M" first="M Amélia" last="Martins-Loução">M Amélia Martins-Loução</name><affiliation><nlm:affiliation>Departamento de Biologia Vegetal, Faculdade de Ciências, Centro de Ecologia e Biologia Vegetal, Universidade de Lisboa, Lisboa, 1749-016, Portugal.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Museu Nacional de História Natural, Jardim Botânico, 1250-102, Lisboa, Portugal.</nlm:affiliation></affiliation></author><author><name sortKey="Freitas, Helena" sort="Freitas, Helena" uniqKey="Freitas H" first="Helena" last="Freitas">Helena Freitas</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17710447</idno><idno type="pmid">17710447</idno><idno type="doi">10.1007/s00572-007-0145-2</idno><idno type="wicri:Area/Main/Corpus">002E59</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002E59</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic diversity and differential in vitro responses to Ni in Cenococcum geophilum isolates from serpentine soils in Portugal.</title><author><name sortKey="Goncalves, Susana C" sort="Goncalves, Susana C" uniqKey="Goncalves S" first="Susana C" last="Gonçalves">Susana C. Gonçalves</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal. scgoncal@ci.uc.pt.</nlm:affiliation></affiliation></author><author><name sortKey="Portugal, Ant Nio" sort="Portugal, Ant Nio" uniqKey="Portugal A" first="Ant Nio" last="Portugal">Ant Nio Portugal</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</nlm:affiliation></affiliation></author><author><name sortKey="Goncalves, M Teresa" sort="Goncalves, M Teresa" uniqKey="Goncalves M" first="M Teresa" last="Gonçalves">M Teresa Gonçalves</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</nlm:affiliation></affiliation></author><author><name sortKey="Vieira, Rita" sort="Vieira, Rita" uniqKey="Vieira R" first="Rita" last="Vieira">Rita Vieira</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</nlm:affiliation></affiliation></author><author><name sortKey="Martins Loucao, M Amelia" sort="Martins Loucao, M Amelia" uniqKey="Martins Loucao M" first="M Amélia" last="Martins-Loução">M Amélia Martins-Loução</name><affiliation><nlm:affiliation>Departamento de Biologia Vegetal, Faculdade de Ciências, Centro de Ecologia e Biologia Vegetal, Universidade de Lisboa, Lisboa, 1749-016, Portugal.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Museu Nacional de História Natural, Jardim Botânico, 1250-102, Lisboa, Portugal.</nlm:affiliation></affiliation></author><author><name sortKey="Freitas, Helena" sort="Freitas, Helena" uniqKey="Freitas H" first="Helena" last="Freitas">Helena Freitas</name><affiliation><nlm:affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="ISSN">0940-6360</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amplified Fragment Length Polymorphism Analysis (MeSH)</term><term>Ascomycota (drug effects)</term><term>Ascomycota (genetics)</term><term>Ascomycota (isolation & purification)</term><term>Ascomycota (metabolism)</term><term>DNA, Fungal (chemistry)</term><term>DNA, Fungal (genetics)</term><term>Genetic Variation (MeSH)</term><term>Mycorrhizae (drug effects)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (isolation & purification)</term><term>Mycorrhizae (metabolism)</term><term>Nickel (pharmacology)</term><term>Portugal (MeSH)</term><term>Quercus (microbiology)</term><term>Soil Microbiology (MeSH)</term><term>Trees (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Portugal</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>DNA, Fungal</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Quercus</term><term>Trees</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Nickel</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amplified Fragment Length Polymorphism Analysis</term><term>Genetic Variation</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Amplified fragment length polymorphism (AFLP) analysis was used to investigate the genetic diversity in isolates of the ectomycorrhizal fungus Cenococcum geophilum from serpentine and non-serpentine soils in Portugal. A high degree of genetic diversity was found among C. geophilum isolates; AFLP fingerprints showed that all the isolates were genetically distinct. We also assessed the in vitro Ni sensitivity in three serpentine isolates and one non-serpentine isolate. Only the non-serpentine isolate was significantly affected by the addition of Ni to the growth medium. At 30 microg g(-1) Ni, radial growth rate and biomass accumulation decreased to 73.3 and 71.6% of control, respectively, a highly significant inhibitory effect. Nickel at this concentration had no significant inhibitory effect on serpentine isolates, and so the fitness of serpentine isolates, as evaluated by radial growth rate and biomass yield, is likely unaffected by Ni in the field. In all isolates, the Ni concentration in the mycelia increased with increasing Ni concentration in the growth medium, but two profiles of Ni accumulation were identified. One serpentine isolate showed a linear trend of Ni accumulation. At the highest Ni exposure, the concentration of Ni in the mycelium of this isolate was in the hyperaccumulation range for Ni as defined for higher plants. In the remaining isolates, Ni accumulation was less pronounced and seems to approach a plateau at 30 microg g(-1) Ni. Because two profiles of Ni accumulation emerged among our Ni-insensitive serpentine isolates, this result suggests that different Ni detoxification pathways may be operating. The non-serpentine isolate whose growth was significantly affected by Ni was separated from the other isolates in the genetic analysis, suggesting a genetic basis for the Ni-sensitivity trait. This hypothesis is further supported by the fact that all isolates were maintained on medium without added Ni to avoid carry-over effects. However, because AFLP analysis failed to distinguish between serpentine and non-serpentine isolates, we cannot conclude that Ni insensitivity among our serpentine isolates is due to evolutionary adaptation. Screening a larger number of isolates, from different geographical origins and environments, should clarify the relationships between genetic diversity, morphology, and physiology in this important species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17710447</PMID><DateCompleted><Year>2008</Year><Month>06</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0940-6360</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>8</Issue><PubDate><Year>2007</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Genetic diversity and differential in vitro responses to Ni in Cenococcum geophilum isolates from serpentine soils in Portugal.</ArticleTitle><Pagination><MedlinePgn>677-686</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-007-0145-2</ELocationID><Abstract><AbstractText>Amplified fragment length polymorphism (AFLP) analysis was used to investigate the genetic diversity in isolates of the ectomycorrhizal fungus Cenococcum geophilum from serpentine and non-serpentine soils in Portugal. A high degree of genetic diversity was found among C. geophilum isolates; AFLP fingerprints showed that all the isolates were genetically distinct. We also assessed the in vitro Ni sensitivity in three serpentine isolates and one non-serpentine isolate. Only the non-serpentine isolate was significantly affected by the addition of Ni to the growth medium. At 30 microg g(-1) Ni, radial growth rate and biomass accumulation decreased to 73.3 and 71.6% of control, respectively, a highly significant inhibitory effect. Nickel at this concentration had no significant inhibitory effect on serpentine isolates, and so the fitness of serpentine isolates, as evaluated by radial growth rate and biomass yield, is likely unaffected by Ni in the field. In all isolates, the Ni concentration in the mycelia increased with increasing Ni concentration in the growth medium, but two profiles of Ni accumulation were identified. One serpentine isolate showed a linear trend of Ni accumulation. At the highest Ni exposure, the concentration of Ni in the mycelium of this isolate was in the hyperaccumulation range for Ni as defined for higher plants. In the remaining isolates, Ni accumulation was less pronounced and seems to approach a plateau at 30 microg g(-1) Ni. Because two profiles of Ni accumulation emerged among our Ni-insensitive serpentine isolates, this result suggests that different Ni detoxification pathways may be operating. The non-serpentine isolate whose growth was significantly affected by Ni was separated from the other isolates in the genetic analysis, suggesting a genetic basis for the Ni-sensitivity trait. This hypothesis is further supported by the fact that all isolates were maintained on medium without added Ni to avoid carry-over effects. However, because AFLP analysis failed to distinguish between serpentine and non-serpentine isolates, we cannot conclude that Ni insensitivity among our serpentine isolates is due to evolutionary adaptation. Screening a larger number of isolates, from different geographical origins and environments, should clarify the relationships between genetic diversity, morphology, and physiology in this important species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gonçalves</LastName><ForeName>Susana C</ForeName><Initials>SC</Initials><AffiliationInfo><Affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal. scgoncal@ci.uc.pt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Portugal</LastName><ForeName>António</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gonçalves</LastName><ForeName>M Teresa</ForeName><Initials>MT</Initials><AffiliationInfo><Affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vieira</LastName><ForeName>Rita</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martins-Loução</LastName><ForeName>M Amélia</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Departamento de Biologia Vegetal, Faculdade de Ciências, Centro de Ecologia e Biologia Vegetal, Universidade de Lisboa, Lisboa, 1749-016, Portugal.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Museu Nacional de História Natural, Jardim Botânico, 1250-102, Lisboa, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Freitas</LastName><ForeName>Helena</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Centro de Ecologia Funcional, Departamento de Botânica, Universidade de Coimbra, 3000-456, Coimbra, Portugal.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>08</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>4FLT4T3WUN</RegistryNumber><NameOfSubstance UI="C029938">nickel sulfate</NameOfSubstance></Chemical><Chemical><RegistryNumber>7OV03QG267</RegistryNumber><NameOfSubstance UI="D009532">Nickel</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D054458" MajorTopicYN="N">Amplified Fragment Length Polymorphism Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009532" MajorTopicYN="N">Nickel</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011174" MajorTopicYN="N" Type="Geographic">Portugal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029963" MajorTopicYN="N">Quercus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil 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