Uvitex2B: a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots.
Identifieur interne : 002432 ( Main/Corpus ); précédent : 002431; suivant : 002433Uvitex2B: a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots.
Auteurs : Nathalie Diagne ; Jacques Escoute ; Marc Lartaud ; Jean Luc Verdeil ; Claudine Franche ; Aboubacry Kane ; Didier Bogusz ; Diegane Diouf ; Robin Duponnois ; Sergio SvistoonoffSource :
- Mycorrhiza [ 1432-1890 ] ; 2011.
English descriptors
- KwdEn :
- Daucus carota (microbiology), Fluorescent Dyes (chemistry), Fungi (chemistry), Glomeromycota (chemistry), Glomeromycota (isolation & purification), Magnoliopsida (microbiology), Medicago truncatula (microbiology), Mycorrhizae (chemistry), Mycorrhizae (isolation & purification), Plant Roots (microbiology), Staining and Labeling (instrumentation), Staining and Labeling (methods).
- MESH :
- chemical , chemistry : Fluorescent Dyes.
- chemistry : Fungi, Glomeromycota, Mycorrhizae.
- instrumentation : Staining and Labeling.
- isolation & purification : Glomeromycota, Mycorrhizae.
- methods : Staining and Labeling.
- microbiology : Daucus carota, Magnoliopsida, Medicago truncatula, Plant Roots.
Abstract
The study of arbuscular mycorrhiza often requires the staining of fungal structures using specific dyes. Fluorescent dyes such as acid fuchsin and wheat germ agglutinin conjugates give excellent results, but these compounds are either hazardous or very expensive. Here, we show that a safer and inexpensive dye, Uvitex2B, can be efficiently used to stain intraradical fungal structures formed by the arbuscular mycorrhizal fungus Glomus intraradices in three plant species: carrot, Casuarina equisetifolia, and Medicago truncatula. The intensity and stability of Uvitex2B allow the acquisition of high-quality images using not only confocal laser scanning microscopy but also epifluorescence microscopy coupled with image deconvolution. Furthermore, we demonstrate that Uvitex2B and β-glucuronidase staining are compatible and can thus be used to reveal arbuscular mycorrhizal structures in the context of promoter activation analysis.
DOI: 10.1007/s00572-010-0357-8
PubMed: 21225294
Links to Exploration step
pubmed:21225294Le document en format XML
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uniqKey="Franche C" first="Claudine" last="Franche">Claudine Franche</name></author><author><name sortKey="Kane, Aboubacry" sort="Kane, Aboubacry" uniqKey="Kane A" first="Aboubacry" last="Kane">Aboubacry Kane</name></author><author><name sortKey="Bogusz, Didier" sort="Bogusz, Didier" uniqKey="Bogusz D" first="Didier" last="Bogusz">Didier Bogusz</name></author><author><name sortKey="Diouf, Diegane" sort="Diouf, Diegane" uniqKey="Diouf D" first="Diegane" last="Diouf">Diegane Diouf</name></author><author><name sortKey="Duponnois, Robin" sort="Duponnois, Robin" uniqKey="Duponnois R" first="Robin" last="Duponnois">Robin Duponnois</name></author><author><name sortKey="Svistoonoff, Sergio" sort="Svistoonoff, Sergio" uniqKey="Svistoonoff S" first="Sergio" last="Svistoonoff">Sergio Svistoonoff</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21225294</idno><idno type="pmid">21225294</idno><idno 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sortKey="Verdeil, Jean Luc" sort="Verdeil, Jean Luc" uniqKey="Verdeil J" first="Jean Luc" last="Verdeil">Jean Luc Verdeil</name></author><author><name sortKey="Franche, Claudine" sort="Franche, Claudine" uniqKey="Franche C" first="Claudine" last="Franche">Claudine Franche</name></author><author><name sortKey="Kane, Aboubacry" sort="Kane, Aboubacry" uniqKey="Kane A" first="Aboubacry" last="Kane">Aboubacry Kane</name></author><author><name sortKey="Bogusz, Didier" sort="Bogusz, Didier" uniqKey="Bogusz D" first="Didier" last="Bogusz">Didier Bogusz</name></author><author><name sortKey="Diouf, Diegane" sort="Diouf, Diegane" uniqKey="Diouf D" first="Diegane" last="Diouf">Diegane Diouf</name></author><author><name sortKey="Duponnois, Robin" sort="Duponnois, Robin" uniqKey="Duponnois R" first="Robin" last="Duponnois">Robin Duponnois</name></author><author><name sortKey="Svistoonoff, Sergio" sort="Svistoonoff, Sergio" uniqKey="Svistoonoff S" first="Sergio" last="Svistoonoff">Sergio Svistoonoff</name></author></analytic><series><title level="j">Mycorrhiza</title><idno type="eISSN">1432-1890</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Daucus carota (microbiology)</term><term>Fluorescent Dyes (chemistry)</term><term>Fungi (chemistry)</term><term>Glomeromycota (chemistry)</term><term>Glomeromycota (isolation & purification)</term><term>Magnoliopsida (microbiology)</term><term>Medicago truncatula (microbiology)</term><term>Mycorrhizae (chemistry)</term><term>Mycorrhizae (isolation & purification)</term><term>Plant Roots (microbiology)</term><term>Staining and Labeling (instrumentation)</term><term>Staining and Labeling (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fluorescent Dyes</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Fungi</term><term>Glomeromycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Glomeromycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Daucus carota</term><term>Magnoliopsida</term><term>Medicago truncatula</term><term>Plant Roots</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The study of arbuscular mycorrhiza often requires the staining of fungal structures using specific dyes. Fluorescent dyes such as acid fuchsin and wheat germ agglutinin conjugates give excellent results, but these compounds are either hazardous or very expensive. Here, we show that a safer and inexpensive dye, Uvitex2B, can be efficiently used to stain intraradical fungal structures formed by the arbuscular mycorrhizal fungus Glomus intraradices in three plant species: carrot, Casuarina equisetifolia, and Medicago truncatula. The intensity and stability of Uvitex2B allow the acquisition of high-quality images using not only confocal laser scanning microscopy but also epifluorescence microscopy coupled with image deconvolution. Furthermore, we demonstrate that Uvitex2B and β-glucuronidase staining are compatible and can thus be used to reveal arbuscular mycorrhizal structures in the context of promoter activation analysis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21225294</PMID><DateCompleted><Year>2011</Year><Month>07</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1890</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>4</Issue><PubDate><Year>2011</Year><Month>May</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Uvitex2B: a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots.</ArticleTitle><Pagination><MedlinePgn>315-21</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-010-0357-8</ELocationID><Abstract><AbstractText>The study of arbuscular mycorrhiza often requires the staining of fungal structures using specific dyes. Fluorescent dyes such as acid fuchsin and wheat germ agglutinin conjugates give excellent results, but these compounds are either hazardous or very expensive. Here, we show that a safer and inexpensive dye, Uvitex2B, can be efficiently used to stain intraradical fungal structures formed by the arbuscular mycorrhizal fungus Glomus intraradices in three plant species: carrot, Casuarina equisetifolia, and Medicago truncatula. The intensity and stability of Uvitex2B allow the acquisition of high-quality images using not only confocal laser scanning microscopy but also epifluorescence microscopy coupled with image deconvolution. Furthermore, we demonstrate that Uvitex2B and β-glucuronidase staining are compatible and can thus be used to reveal arbuscular mycorrhizal structures in the context of promoter activation analysis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Diagne</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Département de Biologie Végétale, Université Cheikh Anta Diop (UCAD), Dakar, Senegal. nathalie.diagne@ird.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Escoute</LastName><ForeName>Jacques</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Lartaud</LastName><ForeName>Marc</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Verdeil</LastName><ForeName>Jean Luc</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Franche</LastName><ForeName>Claudine</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Kane</LastName><ForeName>Aboubacry</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bogusz</LastName><ForeName>Didier</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Diouf</LastName><ForeName>Diegane</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Duponnois</LastName><ForeName>Robin</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Svistoonoff</LastName><ForeName>Sergio</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>01</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018552" MajorTopicYN="N">Daucus carota</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005456" MajorTopicYN="N">Fluorescent Dyes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000737" 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Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>06</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>1</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>1</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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