PvRbohB negatively regulates Rhizophagus irregularis colonization in Phaseolus vulgaris.
Identifieur interne : 001B82 ( Main/Corpus ); précédent : 001B81; suivant : 001B83PvRbohB negatively regulates Rhizophagus irregularis colonization in Phaseolus vulgaris.
Auteurs : Manoj-Kumar Arthikala ; Jesús Montiel ; Noreide Nava ; Olivia Santana ; Rosana Sánchez-L Pez ; Luis Cárdenas ; Carmen QuintoSource :
- Plant & cell physiology [ 1471-9053 ] ; 2013.
English descriptors
- KwdEn :
- Down-Regulation (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Reporter (MeSH), Glomeromycota (physiology), Mycorrhizae (cytology), Mycorrhizae (genetics), Mycorrhizae (physiology), NADPH Oxidases (genetics), NADPH Oxidases (metabolism), Phaseolus (cytology), Phaseolus (genetics), Phaseolus (microbiology), Phaseolus (physiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (cytology), Plant Roots (genetics), Plant Roots (microbiology), Plant Roots (physiology), RNA Interference (MeSH), Reactive Oxygen Species (analysis), Reactive Oxygen Species (metabolism), Symbiosis (MeSH).
- MESH :
- chemical , analysis : Reactive Oxygen Species.
- chemical , genetics : NADPH Oxidases, Plant Proteins.
- cytology : Mycorrhizae, Phaseolus, Plant Roots.
- genetics : Mycorrhizae, Phaseolus, Plant Roots.
- chemical , metabolism : NADPH Oxidases, Plant Proteins, Reactive Oxygen Species.
- microbiology : Phaseolus, Plant Roots.
- physiology : Glomeromycota, Mycorrhizae, Phaseolus, Plant Roots.
- Down-Regulation, Gene Expression Regulation, Plant, Genes, Reporter, RNA Interference, Symbiosis.
Abstract
Plant NADPH oxidases (RBOHs) regulate the early stages of rhizobial infection in Phaseolus vulgaris and affect nodule function in Medicago truncatula. In contrast, the role of RBOHs in the plant-arbuscular mycorrhizal (AM) symbiosis and in the regulation of reactive oxygen species (ROS) production during the establishment of the AM interaction is largely unknown. In this study, we assessed the role of P. vulgaris Rboh (PvRbohB) during the symbiosis with the AM fungus, Rhizophagus irregularis. Our results indicate that the PvRbohB transcript is significantly up-regulated in the mycorrhized roots of P. vulgaris. Further, the PvRbohB promoter was found to be active during the invasion of R. irregularis. Down-regulation of PvRbohB transcription by RNAi (RNA interference) silencing resulted in diminished ROS levels in the transgenic mycorrhized roots and induced early hyphal root colonization. Interestingly, the size of appressoria increased in PvRbohB-RNAi roots (760 ± 70.1 µm) relative to controls (251 ± 73.2 µm). Finally, the overall level of mycorrhizal colonization significantly increased in PvRbohB-RNAi roots [48.1 ± 3.3% root length colonization (RLC)] compared with controls (29.4 ± 1.9% RLC). We propose that PvRbohB negatively regulates AM colonization in P. vulgaris.
DOI: 10.1093/pcp/pct089
PubMed: 23788647
Links to Exploration step
pubmed:23788647Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">PvRbohB negatively regulates Rhizophagus irregularis colonization in Phaseolus vulgaris.</title><author><name sortKey="Arthikala, Manoj Kumar" sort="Arthikala, Manoj Kumar" uniqKey="Arthikala M" first="Manoj-Kumar" last="Arthikala">Manoj-Kumar Arthikala</name><affiliation><nlm:affiliation>Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México.</nlm:affiliation></affiliation></author><author><name sortKey="Montiel, Jesus" sort="Montiel, Jesus" uniqKey="Montiel J" first="Jesús" last="Montiel">Jesús Montiel</name></author><author><name sortKey="Nava, Noreide" sort="Nava, Noreide" uniqKey="Nava N" first="Noreide" last="Nava">Noreide Nava</name></author><author><name sortKey="Santana, Olivia" sort="Santana, Olivia" uniqKey="Santana O" first="Olivia" last="Santana">Olivia Santana</name></author><author><name sortKey="Sanchez L Pez, Rosana" sort="Sanchez L Pez, Rosana" uniqKey="Sanchez L Pez R" first="Rosana" last="Sánchez-L Pez">Rosana Sánchez-L Pez</name></author><author><name sortKey="Cardenas, Luis" sort="Cardenas, Luis" uniqKey="Cardenas L" first="Luis" last="Cárdenas">Luis Cárdenas</name></author><author><name sortKey="Quinto, Carmen" sort="Quinto, Carmen" uniqKey="Quinto C" first="Carmen" last="Quinto">Carmen Quinto</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23788647</idno><idno type="pmid">23788647</idno><idno type="doi">10.1093/pcp/pct089</idno><idno type="wicri:Area/Main/Corpus">001B82</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001B82</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">PvRbohB negatively regulates Rhizophagus irregularis colonization in Phaseolus vulgaris.</title><author><name sortKey="Arthikala, Manoj Kumar" sort="Arthikala, Manoj Kumar" uniqKey="Arthikala M" first="Manoj-Kumar" last="Arthikala">Manoj-Kumar Arthikala</name><affiliation><nlm:affiliation>Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México.</nlm:affiliation></affiliation></author><author><name sortKey="Montiel, Jesus" sort="Montiel, Jesus" uniqKey="Montiel J" first="Jesús" last="Montiel">Jesús Montiel</name></author><author><name sortKey="Nava, Noreide" sort="Nava, Noreide" uniqKey="Nava N" first="Noreide" last="Nava">Noreide Nava</name></author><author><name sortKey="Santana, Olivia" sort="Santana, Olivia" uniqKey="Santana O" first="Olivia" last="Santana">Olivia Santana</name></author><author><name sortKey="Sanchez L Pez, Rosana" sort="Sanchez L Pez, Rosana" uniqKey="Sanchez L Pez R" first="Rosana" last="Sánchez-L Pez">Rosana Sánchez-L Pez</name></author><author><name sortKey="Cardenas, Luis" sort="Cardenas, Luis" uniqKey="Cardenas L" first="Luis" last="Cárdenas">Luis Cárdenas</name></author><author><name sortKey="Quinto, Carmen" sort="Quinto, Carmen" uniqKey="Quinto C" first="Carmen" last="Quinto">Carmen Quinto</name></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="eISSN">1471-9053</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Down-Regulation (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Glomeromycota (physiology)</term><term>Mycorrhizae (cytology)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (physiology)</term><term>NADPH Oxidases (genetics)</term><term>NADPH Oxidases (metabolism)</term><term>Phaseolus (cytology)</term><term>Phaseolus (genetics)</term><term>Phaseolus (microbiology)</term><term>Phaseolus (physiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (cytology)</term><term>Plant Roots (genetics)</term><term>Plant Roots (microbiology)</term><term>Plant Roots (physiology)</term><term>RNA Interference (MeSH)</term><term>Reactive Oxygen Species (analysis)</term><term>Reactive Oxygen Species (metabolism)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>NADPH Oxidases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Mycorrhizae</term><term>Phaseolus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycorrhizae</term><term>Phaseolus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>NADPH Oxidases</term><term>Plant Proteins</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Phaseolus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Glomeromycota</term><term>Mycorrhizae</term><term>Phaseolus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Down-Regulation</term><term>Gene Expression Regulation, Plant</term><term>Genes, Reporter</term><term>RNA Interference</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant NADPH oxidases (RBOHs) regulate the early stages of rhizobial infection in Phaseolus vulgaris and affect nodule function in Medicago truncatula. In contrast, the role of RBOHs in the plant-arbuscular mycorrhizal (AM) symbiosis and in the regulation of reactive oxygen species (ROS) production during the establishment of the AM interaction is largely unknown. In this study, we assessed the role of P. vulgaris Rboh (PvRbohB) during the symbiosis with the AM fungus, Rhizophagus irregularis. Our results indicate that the PvRbohB transcript is significantly up-regulated in the mycorrhized roots of P. vulgaris. Further, the PvRbohB promoter was found to be active during the invasion of R. irregularis. Down-regulation of PvRbohB transcription by RNAi (RNA interference) silencing resulted in diminished ROS levels in the transgenic mycorrhized roots and induced early hyphal root colonization. Interestingly, the size of appressoria increased in PvRbohB-RNAi roots (760 ± 70.1 µm) relative to controls (251 ± 73.2 µm). Finally, the overall level of mycorrhizal colonization significantly increased in PvRbohB-RNAi roots [48.1 ± 3.3% root length colonization (RLC)] compared with controls (29.4 ± 1.9% RLC). We propose that PvRbohB negatively regulates AM colonization in P. vulgaris. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23788647</PMID><DateCompleted><Year>2014</Year><Month>06</Month><Day>10</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1471-9053</ISSN><JournalIssue CitedMedium="Internet"><Volume>54</Volume><Issue>8</Issue><PubDate><Year>2013</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>PvRbohB negatively regulates Rhizophagus irregularis colonization in Phaseolus vulgaris.</ArticleTitle><Pagination><MedlinePgn>1391-402</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/pcp/pct089</ELocationID><Abstract><AbstractText>Plant NADPH oxidases (RBOHs) regulate the early stages of rhizobial infection in Phaseolus vulgaris and affect nodule function in Medicago truncatula. In contrast, the role of RBOHs in the plant-arbuscular mycorrhizal (AM) symbiosis and in the regulation of reactive oxygen species (ROS) production during the establishment of the AM interaction is largely unknown. In this study, we assessed the role of P. vulgaris Rboh (PvRbohB) during the symbiosis with the AM fungus, Rhizophagus irregularis. Our results indicate that the PvRbohB transcript is significantly up-regulated in the mycorrhized roots of P. vulgaris. Further, the PvRbohB promoter was found to be active during the invasion of R. irregularis. Down-regulation of PvRbohB transcription by RNAi (RNA interference) silencing resulted in diminished ROS levels in the transgenic mycorrhized roots and induced early hyphal root colonization. Interestingly, the size of appressoria increased in PvRbohB-RNAi roots (760 ± 70.1 µm) relative to controls (251 ± 73.2 µm). Finally, the overall level of mycorrhizal colonization significantly increased in PvRbohB-RNAi roots [48.1 ± 3.3% root length colonization (RLC)] compared with controls (29.4 ± 1.9% RLC). We propose that PvRbohB negatively regulates AM colonization in P. vulgaris. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Arthikala</LastName><ForeName>Manoj-Kumar</ForeName><Initials>MK</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Montiel</LastName><ForeName>Jesús</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Nava</LastName><ForeName>Noreide</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Santana</LastName><ForeName>Olivia</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Sánchez-López</LastName><ForeName>Rosana</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Cárdenas</LastName><ForeName>Luis</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Quinto</LastName><ForeName>Carmen</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>06</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Cell Physiol</MedlineTA><NlmUniqueID>9430925</NlmUniqueID><ISSNLinking>0032-0781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.6.3.-</RegistryNumber><NameOfSubstance UI="D019255">NADPH Oxidases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019255" MajorTopicYN="N">NADPH Oxidases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027805" MajorTopicYN="N">Phaseolus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arbuscular mycorrhiza</Keyword><Keyword MajorTopicYN="N">Common bean</Keyword><Keyword MajorTopicYN="N">ROS</Keyword><Keyword MajorTopicYN="N">Rbohs</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>6</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23788647</ArticleId><ArticleId IdType="pii">pct089</ArticleId><ArticleId IdType="doi">10.1093/pcp/pct089</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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