PvRbohB negatively regulates Rhizophagus irregularis colonization in Phaseolus vulgaris.
Identifieur interne : 001B82 ( Main/Corpus ); précédent : 001B81; suivant : 001B83PvRbohB negatively regulates Rhizophagus irregularis colonization in Phaseolus vulgaris.
Auteurs : Manoj-Kumar Arthikala ; Jesús Montiel ; Noreide Nava ; Olivia Santana ; Rosana Sánchez-L Pez ; Luis Cárdenas ; Carmen QuintoSource :
- Plant & cell physiology [ 1471-9053 ] ; 2013.
English descriptors
- KwdEn :
- Down-Regulation (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Reporter (MeSH), Glomeromycota (physiology), Mycorrhizae (cytology), Mycorrhizae (genetics), Mycorrhizae (physiology), NADPH Oxidases (genetics), NADPH Oxidases (metabolism), Phaseolus (cytology), Phaseolus (genetics), Phaseolus (microbiology), Phaseolus (physiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (cytology), Plant Roots (genetics), Plant Roots (microbiology), Plant Roots (physiology), RNA Interference (MeSH), Reactive Oxygen Species (analysis), Reactive Oxygen Species (metabolism), Symbiosis (MeSH).
- MESH :
- chemical , analysis : Reactive Oxygen Species.
- chemical , genetics : NADPH Oxidases, Plant Proteins.
- cytology : Mycorrhizae, Phaseolus, Plant Roots.
- genetics : Mycorrhizae, Phaseolus, Plant Roots.
- chemical , metabolism : NADPH Oxidases, Plant Proteins, Reactive Oxygen Species.
- microbiology : Phaseolus, Plant Roots.
- physiology : Glomeromycota, Mycorrhizae, Phaseolus, Plant Roots.
- Down-Regulation, Gene Expression Regulation, Plant, Genes, Reporter, RNA Interference, Symbiosis.
Abstract
Plant NADPH oxidases (RBOHs) regulate the early stages of rhizobial infection in Phaseolus vulgaris and affect nodule function in Medicago truncatula. In contrast, the role of RBOHs in the plant-arbuscular mycorrhizal (AM) symbiosis and in the regulation of reactive oxygen species (ROS) production during the establishment of the AM interaction is largely unknown. In this study, we assessed the role of P. vulgaris Rboh (PvRbohB) during the symbiosis with the AM fungus, Rhizophagus irregularis. Our results indicate that the PvRbohB transcript is significantly up-regulated in the mycorrhized roots of P. vulgaris. Further, the PvRbohB promoter was found to be active during the invasion of R. irregularis. Down-regulation of PvRbohB transcription by RNAi (RNA interference) silencing resulted in diminished ROS levels in the transgenic mycorrhized roots and induced early hyphal root colonization. Interestingly, the size of appressoria increased in PvRbohB-RNAi roots (760 ± 70.1 µm) relative to controls (251 ± 73.2 µm). Finally, the overall level of mycorrhizal colonization significantly increased in PvRbohB-RNAi roots [48.1 ± 3.3% root length colonization (RLC)] compared with controls (29.4 ± 1.9% RLC). We propose that PvRbohB negatively regulates AM colonization in P. vulgaris.
DOI: 10.1093/pcp/pct089
PubMed: 23788647
Links to Exploration step
pubmed:23788647Le document en format XML
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<author><name sortKey="Arthikala, Manoj Kumar" sort="Arthikala, Manoj Kumar" uniqKey="Arthikala M" first="Manoj-Kumar" last="Arthikala">Manoj-Kumar Arthikala</name>
<affiliation><nlm:affiliation>Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México.</nlm:affiliation>
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<author><name sortKey="Montiel, Jesus" sort="Montiel, Jesus" uniqKey="Montiel J" first="Jesús" last="Montiel">Jesús Montiel</name>
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<author><name sortKey="Nava, Noreide" sort="Nava, Noreide" uniqKey="Nava N" first="Noreide" last="Nava">Noreide Nava</name>
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<author><name sortKey="Santana, Olivia" sort="Santana, Olivia" uniqKey="Santana O" first="Olivia" last="Santana">Olivia Santana</name>
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<author><name sortKey="Sanchez L Pez, Rosana" sort="Sanchez L Pez, Rosana" uniqKey="Sanchez L Pez R" first="Rosana" last="Sánchez-L Pez">Rosana Sánchez-L Pez</name>
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<author><name sortKey="Santana, Olivia" sort="Santana, Olivia" uniqKey="Santana O" first="Olivia" last="Santana">Olivia Santana</name>
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<author><name sortKey="Sanchez L Pez, Rosana" sort="Sanchez L Pez, Rosana" uniqKey="Sanchez L Pez R" first="Rosana" last="Sánchez-L Pez">Rosana Sánchez-L Pez</name>
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<series><title level="j">Plant & cell physiology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Down-Regulation (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Reporter (MeSH)</term>
<term>Glomeromycota (physiology)</term>
<term>Mycorrhizae (cytology)</term>
<term>Mycorrhizae (genetics)</term>
<term>Mycorrhizae (physiology)</term>
<term>NADPH Oxidases (genetics)</term>
<term>NADPH Oxidases (metabolism)</term>
<term>Phaseolus (cytology)</term>
<term>Phaseolus (genetics)</term>
<term>Phaseolus (microbiology)</term>
<term>Phaseolus (physiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plant Roots (cytology)</term>
<term>Plant Roots (genetics)</term>
<term>Plant Roots (microbiology)</term>
<term>Plant Roots (physiology)</term>
<term>RNA Interference (MeSH)</term>
<term>Reactive Oxygen Species (analysis)</term>
<term>Reactive Oxygen Species (metabolism)</term>
<term>Symbiosis (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Reactive Oxygen Species</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>NADPH Oxidases</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Mycorrhizae</term>
<term>Phaseolus</term>
<term>Plant Roots</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycorrhizae</term>
<term>Phaseolus</term>
<term>Plant Roots</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>NADPH Oxidases</term>
<term>Plant Proteins</term>
<term>Reactive Oxygen Species</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Phaseolus</term>
<term>Plant Roots</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Glomeromycota</term>
<term>Mycorrhizae</term>
<term>Phaseolus</term>
<term>Plant Roots</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Down-Regulation</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genes, Reporter</term>
<term>RNA Interference</term>
<term>Symbiosis</term>
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<front><div type="abstract" xml:lang="en">Plant NADPH oxidases (RBOHs) regulate the early stages of rhizobial infection in Phaseolus vulgaris and affect nodule function in Medicago truncatula. In contrast, the role of RBOHs in the plant-arbuscular mycorrhizal (AM) symbiosis and in the regulation of reactive oxygen species (ROS) production during the establishment of the AM interaction is largely unknown. In this study, we assessed the role of P. vulgaris Rboh (PvRbohB) during the symbiosis with the AM fungus, Rhizophagus irregularis. Our results indicate that the PvRbohB transcript is significantly up-regulated in the mycorrhized roots of P. vulgaris. Further, the PvRbohB promoter was found to be active during the invasion of R. irregularis. Down-regulation of PvRbohB transcription by RNAi (RNA interference) silencing resulted in diminished ROS levels in the transgenic mycorrhized roots and induced early hyphal root colonization. Interestingly, the size of appressoria increased in PvRbohB-RNAi roots (760 ± 70.1 µm) relative to controls (251 ± 73.2 µm). Finally, the overall level of mycorrhizal colonization significantly increased in PvRbohB-RNAi roots [48.1 ± 3.3% root length colonization (RLC)] compared with controls (29.4 ± 1.9% RLC). We propose that PvRbohB negatively regulates AM colonization in P. vulgaris. </div>
</front>
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<Title>Plant & cell physiology</Title>
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<ArticleTitle>PvRbohB negatively regulates Rhizophagus irregularis colonization in Phaseolus vulgaris.</ArticleTitle>
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<Abstract><AbstractText>Plant NADPH oxidases (RBOHs) regulate the early stages of rhizobial infection in Phaseolus vulgaris and affect nodule function in Medicago truncatula. In contrast, the role of RBOHs in the plant-arbuscular mycorrhizal (AM) symbiosis and in the regulation of reactive oxygen species (ROS) production during the establishment of the AM interaction is largely unknown. In this study, we assessed the role of P. vulgaris Rboh (PvRbohB) during the symbiosis with the AM fungus, Rhizophagus irregularis. Our results indicate that the PvRbohB transcript is significantly up-regulated in the mycorrhized roots of P. vulgaris. Further, the PvRbohB promoter was found to be active during the invasion of R. irregularis. Down-regulation of PvRbohB transcription by RNAi (RNA interference) silencing resulted in diminished ROS levels in the transgenic mycorrhized roots and induced early hyphal root colonization. Interestingly, the size of appressoria increased in PvRbohB-RNAi roots (760 ± 70.1 µm) relative to controls (251 ± 73.2 µm). Finally, the overall level of mycorrhizal colonization significantly increased in PvRbohB-RNAi roots [48.1 ± 3.3% root length colonization (RLC)] compared with controls (29.4 ± 1.9% RLC). We propose that PvRbohB negatively regulates AM colonization in P. vulgaris. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Arthikala</LastName>
<ForeName>Manoj-Kumar</ForeName>
<Initials>MK</Initials>
<AffiliationInfo><Affiliation>Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México.</Affiliation>
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<Author ValidYN="Y"><LastName>Montiel</LastName>
<ForeName>Jesús</ForeName>
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<Author ValidYN="Y"><LastName>Nava</LastName>
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<Author ValidYN="Y"><LastName>Santana</LastName>
<ForeName>Olivia</ForeName>
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<Author ValidYN="Y"><LastName>Sánchez-López</LastName>
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<Author ValidYN="Y"><LastName>Cárdenas</LastName>
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<MeshHeading><DescriptorName UI="D027805" MajorTopicYN="N">Phaseolus</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
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<MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName>
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<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arbuscular mycorrhiza</Keyword>
<Keyword MajorTopicYN="N">Common bean</Keyword>
<Keyword MajorTopicYN="N">ROS</Keyword>
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