VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.
Identifieur interne : 001526 ( Main/Corpus ); précédent : 001525; suivant : 001527VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.
Auteurs : Mette Gr NlundSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2015.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (physiology), Gene Expression Regulation, Plant (MeSH), Gene Knockdown Techniques (methods), Gene Silencing (MeSH), Mycorrhizae (physiology), Peas (genetics), Peas (microbiology), Plant Proteins (genetics), Plant Viruses (genetics), Plant Viruses (physiology), Rhizobium leguminosarum (physiology), Symbiosis (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Peas, Plant Viruses.
- methods : Gene Knockdown Techniques.
- microbiology : Peas.
- physiology : Agrobacterium tumefaciens, Mycorrhizae, Plant Viruses, Rhizobium leguminosarum.
- Gene Expression Regulation, Plant, Gene Silencing, Symbiosis.
Abstract
Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.
DOI: 10.1007/978-1-4939-2453-0_19
PubMed: 25740371
Links to Exploration step
pubmed:25740371Le document en format XML
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<affiliation><nlm:affiliation>Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark, metg@kt.dtu.dk.</nlm:affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.</title>
<author><name sortKey="Gr Nlund, Mette" sort="Gr Nlund, Mette" uniqKey="Gr Nlund M" first="Mette" last="Gr Nlund">Mette Gr Nlund</name>
<affiliation><nlm:affiliation>Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark, metg@kt.dtu.dk.</nlm:affiliation>
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<series><title level="j">Methods in molecular biology (Clifton, N.J.)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (physiology)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Gene Knockdown Techniques (methods)</term>
<term>Gene Silencing (MeSH)</term>
<term>Mycorrhizae (physiology)</term>
<term>Peas (genetics)</term>
<term>Peas (microbiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Viruses (genetics)</term>
<term>Plant Viruses (physiology)</term>
<term>Rhizobium leguminosarum (physiology)</term>
<term>Symbiosis (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Peas</term>
<term>Plant Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Knockdown Techniques</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Peas</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Mycorrhizae</term>
<term>Plant Viruses</term>
<term>Rhizobium leguminosarum</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Gene Silencing</term>
<term>Symbiosis</term>
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<front><div type="abstract" xml:lang="en">Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.</div>
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<Title>Methods in molecular biology (Clifton, N.J.)</Title>
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<Abstract><AbstractText>Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.</AbstractText>
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<MeshHeading><DescriptorName UI="D016961" MajorTopicYN="N">Rhizobium leguminosarum</DescriptorName>
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