VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.
Identifieur interne : 001526 ( Main/Corpus ); précédent : 001525; suivant : 001527VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.
Auteurs : Mette Gr NlundSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2015.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (physiology), Gene Expression Regulation, Plant (MeSH), Gene Knockdown Techniques (methods), Gene Silencing (MeSH), Mycorrhizae (physiology), Peas (genetics), Peas (microbiology), Plant Proteins (genetics), Plant Viruses (genetics), Plant Viruses (physiology), Rhizobium leguminosarum (physiology), Symbiosis (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Peas, Plant Viruses.
- methods : Gene Knockdown Techniques.
- microbiology : Peas.
- physiology : Agrobacterium tumefaciens, Mycorrhizae, Plant Viruses, Rhizobium leguminosarum.
- Gene Expression Regulation, Plant, Gene Silencing, Symbiosis.
Abstract
Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.
DOI: 10.1007/978-1-4939-2453-0_19
PubMed: 25740371
Links to Exploration step
pubmed:25740371Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.</title><author><name sortKey="Gr Nlund, Mette" sort="Gr Nlund, Mette" uniqKey="Gr Nlund M" first="Mette" last="Gr Nlund">Mette Gr Nlund</name><affiliation><nlm:affiliation>Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark, metg@kt.dtu.dk.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25740371</idno><idno type="pmid">25740371</idno><idno type="doi">10.1007/978-1-4939-2453-0_19</idno><idno type="wicri:Area/Main/Corpus">001526</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001526</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.</title><author><name sortKey="Gr Nlund, Mette" sort="Gr Nlund, Mette" uniqKey="Gr Nlund M" first="Mette" last="Gr Nlund">Mette Gr Nlund</name><affiliation><nlm:affiliation>Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark, metg@kt.dtu.dk.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (physiology)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Gene Knockdown Techniques (methods)</term><term>Gene Silencing (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Peas (genetics)</term><term>Peas (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Viruses (genetics)</term><term>Plant Viruses (physiology)</term><term>Rhizobium leguminosarum (physiology)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Peas</term><term>Plant Viruses</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Knockdown Techniques</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Peas</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Mycorrhizae</term><term>Plant Viruses</term><term>Rhizobium leguminosarum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Gene Silencing</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25740371</PMID><DateCompleted><Year>2015</Year><Month>11</Month><Day>20</Day></DateCompleted><DateRevised><Year>2015</Year><Month>03</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1287</Volume><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants.</ArticleTitle><Pagination><MedlinePgn>255-65</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-2453-0_19</ELocationID><Abstract><AbstractText>Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Grønlund</LastName><ForeName>Mette</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark, metg@kt.dtu.dk.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055785" MajorTopicYN="N">Gene Knockdown Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="Y">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018532" MajorTopicYN="N">Peas</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010942" MajorTopicYN="N">Plant Viruses</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016961" MajorTopicYN="N">Rhizobium leguminosarum</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25740371</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-2453-0_19</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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