A symbiosis-dedicated SYNTAXIN OF PLANTS 13II isoform controls the formation of a stable host-microbe interface in symbiosis.
Identifieur interne : 001093 ( Main/Corpus ); précédent : 001092; suivant : 001094A symbiosis-dedicated SYNTAXIN OF PLANTS 13II isoform controls the formation of a stable host-microbe interface in symbiosis.
Auteurs : Rik Huisman ; Jan Hontelez ; Kirankumar S. Mysore ; Jiangqi Wen ; Ton Bisseling ; Erik LimpensSource :
- The New phytologist [ 1469-8137 ] ; 2016.
English descriptors
- KwdEn :
- Alternative Splicing (genetics), Amino Acid Sequence (MeSH), Medicago truncatula (microbiology), Mycorrhizae (cytology), Mycorrhizae (physiology), Phylogeny (MeSH), Plant Proteins (chemistry), Plant Proteins (metabolism), Protein Isoforms (chemistry), Protein Isoforms (metabolism), Protein Transport (MeSH), Rhizobium (physiology), SNARE Proteins (metabolism), Subcellular Fractions (metabolism), Symbiosis (MeSH).
- MESH :
- chemical , chemistry : Plant Proteins, Protein Isoforms.
- cytology : Mycorrhizae.
- genetics : Alternative Splicing.
- chemical , metabolism : Plant Proteins, Protein Isoforms, SNARE Proteins, Subcellular Fractions.
- microbiology : Medicago truncatula.
- physiology : Mycorrhizae, Rhizobium.
- Amino Acid Sequence, Phylogeny, Protein Transport, Symbiosis.
Abstract
Arbuscular mycorrhizal (AM) fungi and rhizobium bacteria are accommodated in specialized membrane compartments that form a host-microbe interface. To better understand how these interfaces are made, we studied the regulation of exocytosis during interface formation. We used a phylogenetic approach to identify target soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (t-SNAREs) that are dedicated to symbiosis and used cell-specific expression analysis together with protein localization to identify t-SNAREs that are present on the host-microbe interface in Medicago truncatula. We investigated the role of these t-SNAREs during the formation of a host-microbe interface. We showed that multiple syntaxins are present on the peri-arbuscular membrane. From these, we identified SYNTAXIN OF PLANTS 13II (SYP13II) as a t-SNARE that is essential for the formation of a stable symbiotic interface in both AM and rhizobium symbiosis. In most dicot plants, the SYP13II transcript is alternatively spliced, resulting in two isoforms, SYP13IIα and SYP13IIβ. These splice-forms differentially mark functional and degrading arbuscule branches. Our results show that vesicle traffic to the symbiotic interface is specialized and required for its maintenance. Alternative splicing of SYP13II allows plants to replace a t-SNARE involved in traffic to the plasma membrane with a t-SNARE that is more stringent in its localization to functional arbuscules.
DOI: 10.1111/nph.13973
PubMed: 27110912
Links to Exploration step
pubmed:27110912Le document en format XML
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Mysore</name><affiliation><nlm:affiliation>Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Wen, Jiangqi" sort="Wen, Jiangqi" uniqKey="Wen J" first="Jiangqi" last="Wen">Jiangqi Wen</name><affiliation><nlm:affiliation>Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bisseling, Ton" sort="Bisseling, Ton" uniqKey="Bisseling T" first="Ton" last="Bisseling">Ton Bisseling</name><affiliation><nlm:affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</nlm:affiliation></affiliation></author><author><name sortKey="Limpens, Erik" sort="Limpens, Erik" uniqKey="Limpens E" first="Erik" last="Limpens">Erik Limpens</name><affiliation><nlm:affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27110912</idno><idno type="pmid">27110912</idno><idno type="doi">10.1111/nph.13973</idno><idno type="wicri:Area/Main/Corpus">001093</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001093</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A symbiosis-dedicated SYNTAXIN OF PLANTS 13II isoform controls the formation of a stable host-microbe interface in symbiosis.</title><author><name sortKey="Huisman, Rik" sort="Huisman, Rik" uniqKey="Huisman R" first="Rik" last="Huisman">Rik Huisman</name><affiliation><nlm:affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</nlm:affiliation></affiliation></author><author><name sortKey="Hontelez, Jan" sort="Hontelez, Jan" uniqKey="Hontelez J" first="Jan" last="Hontelez">Jan Hontelez</name><affiliation><nlm:affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</nlm:affiliation></affiliation></author><author><name sortKey="Mysore, Kirankumar S" sort="Mysore, Kirankumar S" uniqKey="Mysore K" first="Kirankumar S" last="Mysore">Kirankumar S. Mysore</name><affiliation><nlm:affiliation>Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Wen, Jiangqi" sort="Wen, Jiangqi" uniqKey="Wen J" first="Jiangqi" last="Wen">Jiangqi Wen</name><affiliation><nlm:affiliation>Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bisseling, Ton" sort="Bisseling, Ton" uniqKey="Bisseling T" first="Ton" last="Bisseling">Ton Bisseling</name><affiliation><nlm:affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</nlm:affiliation></affiliation></author><author><name sortKey="Limpens, Erik" sort="Limpens, Erik" uniqKey="Limpens E" first="Erik" last="Limpens">Erik Limpens</name><affiliation><nlm:affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alternative Splicing (genetics)</term><term>Amino Acid Sequence (MeSH)</term><term>Medicago truncatula (microbiology)</term><term>Mycorrhizae (cytology)</term><term>Mycorrhizae (physiology)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (metabolism)</term><term>Protein Isoforms (chemistry)</term><term>Protein Isoforms (metabolism)</term><term>Protein Transport (MeSH)</term><term>Rhizobium (physiology)</term><term>SNARE Proteins (metabolism)</term><term>Subcellular Fractions (metabolism)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term><term>Protein Isoforms</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Alternative Splicing</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term><term>Protein Isoforms</term><term>SNARE Proteins</term><term>Subcellular Fractions</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term><term>Rhizobium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Phylogeny</term><term>Protein Transport</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arbuscular mycorrhizal (AM) fungi and rhizobium bacteria are accommodated in specialized membrane compartments that form a host-microbe interface. To better understand how these interfaces are made, we studied the regulation of exocytosis during interface formation. We used a phylogenetic approach to identify target soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (t-SNAREs) that are dedicated to symbiosis and used cell-specific expression analysis together with protein localization to identify t-SNAREs that are present on the host-microbe interface in Medicago truncatula. We investigated the role of these t-SNAREs during the formation of a host-microbe interface. We showed that multiple syntaxins are present on the peri-arbuscular membrane. From these, we identified SYNTAXIN OF PLANTS 13II (SYP13II) as a t-SNARE that is essential for the formation of a stable symbiotic interface in both AM and rhizobium symbiosis. In most dicot plants, the SYP13II transcript is alternatively spliced, resulting in two isoforms, SYP13IIα and SYP13IIβ. These splice-forms differentially mark functional and degrading arbuscule branches. Our results show that vesicle traffic to the symbiotic interface is specialized and required for its maintenance. Alternative splicing of SYP13II allows plants to replace a t-SNARE involved in traffic to the plasma membrane with a t-SNARE that is more stringent in its localization to functional arbuscules.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27110912</PMID><DateCompleted><Year>2018</Year><Month>01</Month><Day>26</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>211</Volume><Issue>4</Issue><PubDate><Year>2016</Year><Month>09</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>A symbiosis-dedicated SYNTAXIN OF PLANTS 13II isoform controls the formation of a stable host-microbe interface in symbiosis.</ArticleTitle><Pagination><MedlinePgn>1338-51</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.13973</ELocationID><Abstract><AbstractText>Arbuscular mycorrhizal (AM) fungi and rhizobium bacteria are accommodated in specialized membrane compartments that form a host-microbe interface. To better understand how these interfaces are made, we studied the regulation of exocytosis during interface formation. We used a phylogenetic approach to identify target soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (t-SNAREs) that are dedicated to symbiosis and used cell-specific expression analysis together with protein localization to identify t-SNAREs that are present on the host-microbe interface in Medicago truncatula. We investigated the role of these t-SNAREs during the formation of a host-microbe interface. We showed that multiple syntaxins are present on the peri-arbuscular membrane. From these, we identified SYNTAXIN OF PLANTS 13II (SYP13II) as a t-SNARE that is essential for the formation of a stable symbiotic interface in both AM and rhizobium symbiosis. In most dicot plants, the SYP13II transcript is alternatively spliced, resulting in two isoforms, SYP13IIα and SYP13IIβ. These splice-forms differentially mark functional and degrading arbuscule branches. Our results show that vesicle traffic to the symbiotic interface is specialized and required for its maintenance. Alternative splicing of SYP13II allows plants to replace a t-SNARE involved in traffic to the plasma membrane with a t-SNARE that is more stringent in its localization to functional arbuscules.</AbstractText><CopyrightInformation>© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huisman</LastName><ForeName>Rik</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hontelez</LastName><ForeName>Jan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mysore</LastName><ForeName>Kirankumar S</ForeName><Initials>KS</Initials><AffiliationInfo><Affiliation>Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Jiangqi</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bisseling</LastName><ForeName>Ton</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Limpens</LastName><ForeName>Erik</ForeName><Initials>E</Initials><Identifier Source="ORCID">0000-0002-9668-4085</Identifier><AffiliationInfo><Affiliation>Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, the Netherlands.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020033">Protein Isoforms</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D050600">SNARE Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017398" MajorTopicYN="N">Alternative Splicing</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046913" MajorTopicYN="N">Medicago truncatula</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020033" MajorTopicYN="N">Protein Isoforms</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050600" MajorTopicYN="N">SNARE Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013347" MajorTopicYN="N">Subcellular Fractions</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE)</Keyword><Keyword MajorTopicYN="Y">alternative splicing</Keyword><Keyword MajorTopicYN="Y">arbuscular mycorrhiza (AM)</Keyword><Keyword MajorTopicYN="Y">arbuscule</Keyword><Keyword MajorTopicYN="Y">host-microbe interface</Keyword><Keyword MajorTopicYN="Y">rhizobium</Keyword><Keyword MajorTopicYN="Y">symbiosis</Keyword><Keyword MajorTopicYN="Y">symbiosome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>12</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>03</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27110912</ArticleId><ArticleId IdType="doi">10.1111/nph.13973</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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