X-ray microanalytical studies of mineral elements in the tripartite symbiosis between lima bean, N2-fixing bacteria and mycorrhizal fungi.
Identifieur interne : 000E75 ( Main/Corpus ); précédent : 000E74; suivant : 000E76X-ray microanalytical studies of mineral elements in the tripartite symbiosis between lima bean, N2-fixing bacteria and mycorrhizal fungi.
Auteurs : Bruna Wurr Rodak ; Douglas Siqueira Freitas ; Soraya Marx Bamberg ; Marco Aurélio Carbone Carneiro ; Luiz Roberto Guimarães GuilhermeSource :
- Journal of microbiological methods [ 1872-8359 ] ; 2017.
English descriptors
- KwdEn :
- Electron Probe Microanalysis (MeSH), Fabaceae (chemistry), Fabaceae (microbiology), Microscopy, Electron, Scanning (MeSH), Mycorrhizae (isolation & purification), Nitrogen-Fixing Bacteria (isolation & purification), Particle Size (MeSH), Plant Roots (chemistry), Plant Roots (microbiology), Spectrometry, X-Ray Emission (MeSH), Symbiosis (MeSH), Trace Elements (analysis).
- MESH :
- chemical , analysis : Trace Elements.
- chemistry : Fabaceae, Plant Roots.
- isolation & purification : Mycorrhizae, Nitrogen-Fixing Bacteria.
- microbiology : Fabaceae, Plant Roots.
- Electron Probe Microanalysis, Microscopy, Electron, Scanning, Particle Size, Spectrometry, X-Ray Emission, Symbiosis.
Abstract
The symbiosis between legumes, arbuscular mycorrhizal (AM) fungi, and N2-fixing bacteria (NFB) provides mutual nutritional gains. However, assessing the nutritional status of the microorganisms is a difficult task. A methodology that could assess this status, in situ, could assist managing these organisms in agriculture. This study used X-ray microanalyses to quantify and locate mineral elements in structures formed in a tripartite symbiosis. Lima bean (Phaseolus lunatus L. Walp) was cultivated in pots under greenhouse conditions, to which we have added AM fungal isolates (Glomus macrocarpum and Acaulospora colombiana) and NFB (Bradyrhizobium japonicum) inocula. Uninoculated control plants were also included. Symbionts were evaluated at the onset of flowering. Quantification of the mineral elements in the symbiotic components was performed using energy dispersive X-ray spectroscopy (EDX) and a scanning electron microscopy (SEM) was used to identify structures. EDX analysis detected 13 elements with the most abundant being N, Ca, and Se, occurring in all tissues, Fe in roots, Ni and Al in epidermis and P and Mo in nodules. Elemental quantification in fungal structures was not possible. The distribution of elements was related to their symbiotic function. X-ray microanalysis can be efficiently applied for nutritional diagnosis in tripartite symbiosis.
DOI: 10.1016/j.mimet.2016.11.006
PubMed: 27838542
Links to Exploration step
pubmed:27838542Le document en format XML
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Electronic address: doug20106@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Bamberg, Soraya Marx" sort="Bamberg, Soraya Marx" uniqKey="Bamberg S" first="Soraya Marx" last="Bamberg">Soraya Marx Bamberg</name><affiliation><nlm:affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Carneiro, Marco Aurelio Carbone" sort="Carneiro, Marco Aurelio Carbone" uniqKey="Carneiro M" first="Marco Aurélio Carbone" last="Carneiro">Marco Aurélio Carbone Carneiro</name><affiliation><nlm:affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Guilherme, Luiz Roberto Guimaraes" sort="Guilherme, Luiz Roberto Guimaraes" uniqKey="Guilherme L" first="Luiz Roberto Guimarães" last="Guilherme">Luiz Roberto Guimarães Guilherme</name><affiliation><nlm:affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:27838542</idno><idno type="pmid">27838542</idno><idno type="doi">10.1016/j.mimet.2016.11.006</idno><idno type="wicri:Area/Main/Corpus">000E75</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">X-ray microanalytical studies of mineral elements in the tripartite symbiosis between lima bean, N<sub>2</sub>-fixing bacteria and mycorrhizal fungi.</title><author><name sortKey="Rodak, Bruna Wurr" sort="Rodak, Bruna Wurr" uniqKey="Rodak B" first="Bruna Wurr" last="Rodak">Bruna Wurr Rodak</name><affiliation><nlm:affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Freitas, Douglas Siqueira" sort="Freitas, Douglas Siqueira" uniqKey="Freitas D" first="Douglas Siqueira" last="Freitas">Douglas Siqueira Freitas</name><affiliation><nlm:affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil. Electronic address: doug20106@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Bamberg, Soraya Marx" sort="Bamberg, Soraya Marx" uniqKey="Bamberg S" first="Soraya Marx" last="Bamberg">Soraya Marx Bamberg</name><affiliation><nlm:affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Carneiro, Marco Aurelio Carbone" sort="Carneiro, Marco Aurelio Carbone" uniqKey="Carneiro M" first="Marco Aurélio Carbone" last="Carneiro">Marco Aurélio Carbone Carneiro</name><affiliation><nlm:affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Guilherme, Luiz Roberto Guimaraes" sort="Guilherme, Luiz Roberto Guimaraes" uniqKey="Guilherme L" first="Luiz Roberto Guimarães" last="Guilherme">Luiz Roberto Guimarães Guilherme</name><affiliation><nlm:affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of microbiological methods</title><idno type="eISSN">1872-8359</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electron Probe Microanalysis (MeSH)</term><term>Fabaceae (chemistry)</term><term>Fabaceae (microbiology)</term><term>Microscopy, Electron, Scanning (MeSH)</term><term>Mycorrhizae (isolation & purification)</term><term>Nitrogen-Fixing Bacteria (isolation & purification)</term><term>Particle Size (MeSH)</term><term>Plant Roots (chemistry)</term><term>Plant Roots (microbiology)</term><term>Spectrometry, X-Ray Emission (MeSH)</term><term>Symbiosis (MeSH)</term><term>Trace Elements (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Trace Elements</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Fabaceae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Mycorrhizae</term><term>Nitrogen-Fixing Bacteria</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Fabaceae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electron Probe Microanalysis</term><term>Microscopy, Electron, Scanning</term><term>Particle Size</term><term>Spectrometry, X-Ray Emission</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The symbiosis between legumes, arbuscular mycorrhizal (AM) fungi, and N<sub>2</sub>-fixing bacteria (NFB) provides mutual nutritional gains. However, assessing the nutritional status of the microorganisms is a difficult task. A methodology that could assess this status, in situ, could assist managing these organisms in agriculture. This study used X-ray microanalyses to quantify and locate mineral elements in structures formed in a tripartite symbiosis. Lima bean (Phaseolus lunatus L. Walp) was cultivated in pots under greenhouse conditions, to which we have added AM fungal isolates (Glomus macrocarpum and Acaulospora colombiana) and NFB (Bradyrhizobium japonicum) inocula. Uninoculated control plants were also included. Symbionts were evaluated at the onset of flowering. Quantification of the mineral elements in the symbiotic components was performed using energy dispersive X-ray spectroscopy (EDX) and a scanning electron microscopy (SEM) was used to identify structures. EDX analysis detected 13 elements with the most abundant being N, Ca, and Se, occurring in all tissues, Fe in roots, Ni and Al in epidermis and P and Mo in nodules. Elemental quantification in fungal structures was not possible. The distribution of elements was related to their symbiotic function. X-ray microanalysis can be efficiently applied for nutritional diagnosis in tripartite symbiosis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27838542</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>11</Day></DateCompleted><DateRevised><Year>2018</Year><Month>01</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-8359</ISSN><JournalIssue CitedMedium="Internet"><Volume>132</Volume><PubDate><Year>2017</Year><Month>01</Month></PubDate></JournalIssue><Title>Journal of microbiological methods</Title><ISOAbbreviation>J Microbiol Methods</ISOAbbreviation></Journal><ArticleTitle>X-ray microanalytical studies of mineral elements in the tripartite symbiosis between lima bean, N<sub>2</sub>-fixing bacteria and mycorrhizal fungi.</ArticleTitle><Pagination><MedlinePgn>14-20</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0167-7012(16)30315-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.mimet.2016.11.006</ELocationID><Abstract><AbstractText>The symbiosis between legumes, arbuscular mycorrhizal (AM) fungi, and N<sub>2</sub>-fixing bacteria (NFB) provides mutual nutritional gains. However, assessing the nutritional status of the microorganisms is a difficult task. A methodology that could assess this status, in situ, could assist managing these organisms in agriculture. This study used X-ray microanalyses to quantify and locate mineral elements in structures formed in a tripartite symbiosis. Lima bean (Phaseolus lunatus L. Walp) was cultivated in pots under greenhouse conditions, to which we have added AM fungal isolates (Glomus macrocarpum and Acaulospora colombiana) and NFB (Bradyrhizobium japonicum) inocula. Uninoculated control plants were also included. Symbionts were evaluated at the onset of flowering. Quantification of the mineral elements in the symbiotic components was performed using energy dispersive X-ray spectroscopy (EDX) and a scanning electron microscopy (SEM) was used to identify structures. EDX analysis detected 13 elements with the most abundant being N, Ca, and Se, occurring in all tissues, Fe in roots, Ni and Al in epidermis and P and Mo in nodules. Elemental quantification in fungal structures was not possible. The distribution of elements was related to their symbiotic function. X-ray microanalysis can be efficiently applied for nutritional diagnosis in tripartite symbiosis.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rodak</LastName><ForeName>Bruna Wurr</ForeName><Initials>BW</Initials><AffiliationInfo><Affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Freitas</LastName><ForeName>Douglas Siqueira</ForeName><Initials>DS</Initials><AffiliationInfo><Affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil. Electronic address: doug20106@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bamberg</LastName><ForeName>Soraya Marx</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carneiro</LastName><ForeName>Marco Aurélio Carbone</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guilherme</LastName><ForeName>Luiz Roberto Guimarães</ForeName><Initials>LR</Initials><AffiliationInfo><Affiliation>Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>11</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Microbiol Methods</MedlineTA><NlmUniqueID>8306883</NlmUniqueID><ISSNLinking>0167-7012</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014131">Trace Elements</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004577" MajorTopicYN="N">Electron Probe Microanalysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007887" MajorTopicYN="N">Fabaceae</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008855" MajorTopicYN="N">Microscopy, Electron, Scanning</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000072738" MajorTopicYN="N">Nitrogen-Fixing Bacteria</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010316" MajorTopicYN="N">Particle Size</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013052" MajorTopicYN="N">Spectrometry, X-Ray Emission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014131" MajorTopicYN="N">Trace Elements</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Distribution</Keyword><Keyword MajorTopicYN="Y">EDX mapping</Keyword><Keyword MajorTopicYN="Y">Nutritional status</Keyword><Keyword MajorTopicYN="Y">Phaseolus lunatus</Keyword><Keyword MajorTopicYN="Y">Quantification</Keyword><Keyword MajorTopicYN="Y">SEM micrographs</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>07</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>10</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>11</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>11</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>11</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27838542</ArticleId><ArticleId IdType="pii">S0167-7012(16)30315-3</ArticleId><ArticleId IdType="doi">10.1016/j.mimet.2016.11.006</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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