Three cis-Regulatory Motifs, AuxRE, MYCRS1 and MYCRS2, are Required for Modulating the Auxin- and Mycorrhiza-Responsive Expression of a Tomato GH3 Gene.
Identifieur interne : 000D31 ( Main/Corpus ); précédent : 000D30; suivant : 000D32Three cis-Regulatory Motifs, AuxRE, MYCRS1 and MYCRS2, are Required for Modulating the Auxin- and Mycorrhiza-Responsive Expression of a Tomato GH3 Gene.
Auteurs : Xiao Chen ; Dehua Liao ; Xiaofeng Yang ; Minjie Ji ; Shuangshuang Wang ; Mian Gu ; Aiqun Chen ; Guohua XuSource :
- Plant & cell physiology [ 1471-9053 ] ; 2017.
English descriptors
- KwdEn :
- Gene Expression Regulation, Plant (MeSH), Glucuronidase (genetics), Indoleacetic Acids (metabolism), Lycopersicon esculentum (genetics), Lycopersicon esculentum (microbiology), Mycorrhizae (MeSH), Oryza (genetics), Oryza (microbiology), Plant Proteins (genetics), Plant Roots (genetics), Plant Roots (microbiology), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (MeSH), Regulatory Sequences, Nucleic Acid (MeSH), Response Elements (MeSH), Soybeans (genetics), Soybeans (microbiology), Tobacco (genetics), Tobacco (microbiology).
- MESH :
- chemical , genetics : Glucuronidase, Plant Proteins.
- chemical , metabolism : Indoleacetic Acids.
- genetics : Lycopersicon esculentum, Oryza, Plant Roots, Soybeans, Tobacco.
- microbiology : Lycopersicon esculentum, Oryza, Plant Roots, Soybeans, Tobacco.
- Gene Expression Regulation, Plant, Mycorrhizae, Plants, Genetically Modified, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, Response Elements.
Abstract
Auxin is well known to be a key regulator that acts in almost all physiological processes during plant growth, and in interactions between plants and microbes. However, to date, the regulatory mechanisms underlying auxin-mediated plant-arbuscular mycorrhizal (AM) fungi symbiosis have not been well deciphered. Previously we identified a GH3 gene, SlGH3.4, strongly responsive to both auxin induction and mycorrhizal symbiosis. Here, we reported a refined dissection of the SlGH3.4 promoter activity using the β-glucuronidase (GUS) reporter. The SlGH3.4 promoter could drive GUS expression strongly in mycorrhizal roots of soybean and rice plants, and in IAA-treated soybean roots, but not in IAA-treated rice roots. A promoter deletion assay revealed three cis-acting motifs, i.e. the auxin-responsive element, AuxRE, and two newly identified motifs named MYCRS1 and MYCRS2, involved in the activation of auxin- and AM-mediated expression of SlGH3.4. Deletion of the AuxRE from the SlGH3.4 promoter caused almost complete abolition of GUS staining in response to external IAA induction. Seven repeats of AuxRE fused to the Cauliflower mosaic virus (CaMV) 35S minimal promoter could direct GUS expression in both IAA-treated and AM fungal-colonized roots of tobacco plants. Four repeats of MYCRS1 or MYCRS2 fused to the CaMV35S minimal promoter was sufficient to drive GUS expression in arbuscule-containing cells, but not in IAA-treated tobacco roots. In summary, our results offer new insights into the molecular mechanisms underlying the potential cross-talk between the auxin and the AM regulatory pathways in modulating the expression of AM-responsive GH3 genes in diverse mycorrhizal plants.
DOI: 10.1093/pcp/pcx013
PubMed: 28339724
Links to Exploration step
pubmed:28339724Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Three cis-Regulatory Motifs, AuxRE, MYCRS1 and MYCRS2, are Required for Modulating the Auxin- and Mycorrhiza-Responsive Expression of a Tomato GH3 Gene.</title><author><name sortKey="Chen, Xiao" sort="Chen, Xiao" uniqKey="Chen X" first="Xiao" last="Chen">Xiao Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liao, Dehua" sort="Liao, Dehua" uniqKey="Liao D" first="Dehua" last="Liao">Dehua Liao</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Xiaofeng" sort="Yang, Xiaofeng" uniqKey="Yang X" first="Xiaofeng" last="Yang">Xiaofeng Yang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ji, Minjie" sort="Ji, Minjie" uniqKey="Ji M" first="Minjie" last="Ji">Minjie Ji</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Shuangshuang" sort="Wang, Shuangshuang" uniqKey="Wang S" first="Shuangshuang" last="Wang">Shuangshuang Wang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Gu, Mian" sort="Gu, Mian" uniqKey="Gu M" first="Mian" last="Gu">Mian Gu</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Aiqun" sort="Chen, Aiqun" uniqKey="Chen A" first="Aiqun" last="Chen">Aiqun Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Guohua" sort="Xu, Guohua" uniqKey="Xu G" first="Guohua" last="Xu">Guohua Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>MOA Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28339724</idno><idno type="pmid">28339724</idno><idno type="doi">10.1093/pcp/pcx013</idno><idno type="wicri:Area/Main/Corpus">000D31</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000D31</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Three cis-Regulatory Motifs, AuxRE, MYCRS1 and MYCRS2, are Required for Modulating the Auxin- and Mycorrhiza-Responsive Expression of a Tomato GH3 Gene.</title><author><name sortKey="Chen, Xiao" sort="Chen, Xiao" uniqKey="Chen X" first="Xiao" last="Chen">Xiao Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liao, Dehua" sort="Liao, Dehua" uniqKey="Liao D" first="Dehua" last="Liao">Dehua Liao</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Xiaofeng" sort="Yang, Xiaofeng" uniqKey="Yang X" first="Xiaofeng" last="Yang">Xiaofeng Yang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ji, Minjie" sort="Ji, Minjie" uniqKey="Ji M" first="Minjie" last="Ji">Minjie Ji</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Shuangshuang" sort="Wang, Shuangshuang" uniqKey="Wang S" first="Shuangshuang" last="Wang">Shuangshuang Wang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Gu, Mian" sort="Gu, Mian" uniqKey="Gu M" first="Mian" last="Gu">Mian Gu</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Aiqun" sort="Chen, Aiqun" uniqKey="Chen A" first="Aiqun" last="Chen">Aiqun Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Guohua" sort="Xu, Guohua" uniqKey="Xu G" first="Guohua" last="Xu">Guohua Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>MOA Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="eISSN">1471-9053</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glucuronidase (genetics)</term><term>Indoleacetic Acids (metabolism)</term><term>Lycopersicon esculentum (genetics)</term><term>Lycopersicon esculentum (microbiology)</term><term>Mycorrhizae (MeSH)</term><term>Oryza (genetics)</term><term>Oryza (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Roots (genetics)</term><term>Plant Roots (microbiology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Regulatory Sequences, Nucleic Acid (MeSH)</term><term>Response Elements (MeSH)</term><term>Soybeans (genetics)</term><term>Soybeans (microbiology)</term><term>Tobacco (genetics)</term><term>Tobacco (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucuronidase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Indoleacetic Acids</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lycopersicon esculentum</term><term>Oryza</term><term>Plant Roots</term><term>Soybeans</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lycopersicon esculentum</term><term>Oryza</term><term>Plant Roots</term><term>Soybeans</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Mycorrhizae</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Regulatory Sequences, Nucleic Acid</term><term>Response Elements</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Auxin is well known to be a key regulator that acts in almost all physiological processes during plant growth, and in interactions between plants and microbes. However, to date, the regulatory mechanisms underlying auxin-mediated plant-arbuscular mycorrhizal (AM) fungi symbiosis have not been well deciphered. Previously we identified a GH3 gene, SlGH3.4, strongly responsive to both auxin induction and mycorrhizal symbiosis. Here, we reported a refined dissection of the SlGH3.4 promoter activity using the β-glucuronidase (GUS) reporter. The SlGH3.4 promoter could drive GUS expression strongly in mycorrhizal roots of soybean and rice plants, and in IAA-treated soybean roots, but not in IAA-treated rice roots. A promoter deletion assay revealed three cis-acting motifs, i.e. the auxin-responsive element, AuxRE, and two newly identified motifs named MYCRS1 and MYCRS2, involved in the activation of auxin- and AM-mediated expression of SlGH3.4. Deletion of the AuxRE from the SlGH3.4 promoter caused almost complete abolition of GUS staining in response to external IAA induction. Seven repeats of AuxRE fused to the Cauliflower mosaic virus (CaMV) 35S minimal promoter could direct GUS expression in both IAA-treated and AM fungal-colonized roots of tobacco plants. Four repeats of MYCRS1 or MYCRS2 fused to the CaMV35S minimal promoter was sufficient to drive GUS expression in arbuscule-containing cells, but not in IAA-treated tobacco roots. In summary, our results offer new insights into the molecular mechanisms underlying the potential cross-talk between the auxin and the AM regulatory pathways in modulating the expression of AM-responsive GH3 genes in diverse mycorrhizal plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28339724</PMID><DateCompleted><Year>2017</Year><Month>10</Month><Day>17</Day></DateCompleted><DateRevised><Year>2017</Year><Month>10</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1471-9053</ISSN><JournalIssue CitedMedium="Internet"><Volume>58</Volume><Issue>4</Issue><PubDate><Year>2017</Year><Month>04</Month><Day>01</Day></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>Three cis-Regulatory Motifs, AuxRE, MYCRS1 and MYCRS2, are Required for Modulating the Auxin- and Mycorrhiza-Responsive Expression of a Tomato GH3 Gene.</ArticleTitle><Pagination><MedlinePgn>770-778</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/pcp/pcx013</ELocationID><Abstract><AbstractText>Auxin is well known to be a key regulator that acts in almost all physiological processes during plant growth, and in interactions between plants and microbes. However, to date, the regulatory mechanisms underlying auxin-mediated plant-arbuscular mycorrhizal (AM) fungi symbiosis have not been well deciphered. Previously we identified a GH3 gene, SlGH3.4, strongly responsive to both auxin induction and mycorrhizal symbiosis. Here, we reported a refined dissection of the SlGH3.4 promoter activity using the β-glucuronidase (GUS) reporter. The SlGH3.4 promoter could drive GUS expression strongly in mycorrhizal roots of soybean and rice plants, and in IAA-treated soybean roots, but not in IAA-treated rice roots. A promoter deletion assay revealed three cis-acting motifs, i.e. the auxin-responsive element, AuxRE, and two newly identified motifs named MYCRS1 and MYCRS2, involved in the activation of auxin- and AM-mediated expression of SlGH3.4. Deletion of the AuxRE from the SlGH3.4 promoter caused almost complete abolition of GUS staining in response to external IAA induction. Seven repeats of AuxRE fused to the Cauliflower mosaic virus (CaMV) 35S minimal promoter could direct GUS expression in both IAA-treated and AM fungal-colonized roots of tobacco plants. Four repeats of MYCRS1 or MYCRS2 fused to the CaMV35S minimal promoter was sufficient to drive GUS expression in arbuscule-containing cells, but not in IAA-treated tobacco roots. In summary, our results offer new insights into the molecular mechanisms underlying the potential cross-talk between the auxin and the AM regulatory pathways in modulating the expression of AM-responsive GH3 genes in diverse mycorrhizal plants.</AbstractText><CopyrightInformation>© The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xiao</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liao</LastName><ForeName>Dehua</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiaofeng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ji</LastName><ForeName>Minjie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Shuangshuang</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Mian</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Aiqun</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Guohua</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>MOA Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Cell Physiol</MedlineTA><NlmUniqueID>9430925</NlmUniqueID><ISSNLinking>0032-0781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007210">Indoleacetic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007210" MajorTopicYN="N">Indoleacetic Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012045" MajorTopicYN="N">Regulatory Sequences, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020218" MajorTopicYN="N">Response Elements</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Arbuscular mycorrhiza</Keyword><Keyword MajorTopicYN="Y">Auxin</Keyword><Keyword MajorTopicYN="Y">GUS reporter</Keyword><Keyword MajorTopicYN="Y">SlGH3.4</Keyword><Keyword MajorTopicYN="Y">cis-regulatory motif</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>05</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>01</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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