Ribosomal DNA polymorphisms reveal genetic structure and a phylogeographic pattern in the Burgundy truffle Tuber aestivum Vittad.
Identifieur interne : 000590 ( Main/Corpus ); précédent : 000589; suivant : 000591Ribosomal DNA polymorphisms reveal genetic structure and a phylogeographic pattern in the Burgundy truffle Tuber aestivum Vittad.
Auteurs : Claudia Riccioni ; Andrea Rubini ; Aziz Türko Lu ; Beatrice Belfiori ; Francesco PaolocciSource :
- Mycologia [ 1557-2536 ]
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Fungal, DNA, Intergenic, DNA, Ribosomal.
- geographic : Turkey.
- classification : Mycorrhizae.
- genetics : Mycorrhizae.
- Genetic Variation, Haplotypes, Phylogeny, Phylogeography, Polymorphism, Genetic.
Abstract
Ectomycorrhizal ascomycetes belonging to the genus Tuber produce edible fruiting bodies known as truffles. Tuber aestivum, in particular, is a fungus appreciated worldwide and has a natural distribution throughout Europe. Most of the molecular studies conducted on this species have been focused on the question as to whether or not T. aestivum and the morphologically similar T. uncinatum are conspecific. Conversely, only a handful of studies have assessed the level and distribution of genetic diversity and occurrence of phylogeographic patterns in this species. Here, we analyzed the genetic diversity of T. aestivum over a wide geographic range, performing an extensive sampling of specimens from Turkey, which is novel, to the best of our knowledge. We compared the internal transcribed spacer (ITS) profiles of 45 samples collected in different Turkish areas with those of 144 samples from all over Europe. We identified 63 haplotypes, 32 of which were exclusively present in Turkey. The majority of these haplotyes were also population specific. Haplotype network analysis and statistical tests highlighted the presence of a genetic structure and phylogeographic pattern, with three spatially distinct genetic clusters (northeastern Europe, southern Europe, and Turkey), with Turkey representing a diversity hotspot. Based on these results, we hypothesize the presence of glacial refugia for T. aestivum in Turkey, whereas European populations likely experienced a population bottleneck. The possible occurrence of cryptic species among Turkish T. aestivum samples also emerged. Our results are of practical relevance for the marketing of T. aestivum truffles and mycorrhizal seedlings and the preservation of the biodiversity of this species.
DOI: 10.1080/00275514.2018.1543508
PubMed: 30676256
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Lu">Aziz Türko Lu</name><affiliation><nlm:affiliation>b School of Environmental and Forest Sciences, Box 352100 University of Washington , Seattle , Washington 98195-2100.</nlm:affiliation></affiliation></author><author><name sortKey="Belfiori, Beatrice" sort="Belfiori, Beatrice" uniqKey="Belfiori B" first="Beatrice" last="Belfiori">Beatrice Belfiori</name><affiliation><nlm:affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Paolocci, Francesco" sort="Paolocci, Francesco" uniqKey="Paolocci F" first="Francesco" last="Paolocci">Francesco Paolocci</name><affiliation><nlm:affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019 Jan-Feb</date><idno type="RBID">pubmed:30676256</idno><idno type="pmid">30676256</idno><idno type="doi">10.1080/00275514.2018.1543508</idno><idno type="wicri:Area/Main/Corpus">000590</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000590</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ribosomal DNA polymorphisms reveal genetic structure and a phylogeographic pattern in the Burgundy truffle Tuber aestivum Vittad.</title><author><name sortKey="Riccioni, Claudia" sort="Riccioni, Claudia" uniqKey="Riccioni C" first="Claudia" last="Riccioni">Claudia Riccioni</name><affiliation><nlm:affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Rubini, Andrea" sort="Rubini, Andrea" uniqKey="Rubini A" first="Andrea" last="Rubini">Andrea Rubini</name><affiliation><nlm:affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Turko Lu, Aziz" sort="Turko Lu, Aziz" uniqKey="Turko Lu A" first="Aziz" last="Türko Lu">Aziz Türko Lu</name><affiliation><nlm:affiliation>b School of Environmental and Forest Sciences, Box 352100 University of Washington , Seattle , Washington 98195-2100.</nlm:affiliation></affiliation></author><author><name sortKey="Belfiori, Beatrice" sort="Belfiori, Beatrice" uniqKey="Belfiori B" first="Beatrice" last="Belfiori">Beatrice Belfiori</name><affiliation><nlm:affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Paolocci, Francesco" sort="Paolocci, Francesco" uniqKey="Paolocci F" first="Francesco" last="Paolocci">Francesco Paolocci</name><affiliation><nlm:affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycologia</title><idno type="eISSN">1557-2536</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Fungal (genetics)</term><term>DNA, Intergenic (genetics)</term><term>DNA, Ribosomal (genetics)</term><term>Genetic Variation (MeSH)</term><term>Haplotypes (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Phylogeny (MeSH)</term><term>Phylogeography (MeSH)</term><term>Polymorphism, Genetic (MeSH)</term><term>Turkey (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term><term>DNA, Intergenic</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Turkey</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genetic Variation</term><term>Haplotypes</term><term>Phylogeny</term><term>Phylogeography</term><term>Polymorphism, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ectomycorrhizal ascomycetes belonging to the genus Tuber produce edible fruiting bodies known as truffles. Tuber aestivum, in particular, is a fungus appreciated worldwide and has a natural distribution throughout Europe. Most of the molecular studies conducted on this species have been focused on the question as to whether or not T. aestivum and the morphologically similar T. uncinatum are conspecific. Conversely, only a handful of studies have assessed the level and distribution of genetic diversity and occurrence of phylogeographic patterns in this species. Here, we analyzed the genetic diversity of T. aestivum over a wide geographic range, performing an extensive sampling of specimens from Turkey, which is novel, to the best of our knowledge. We compared the internal transcribed spacer (ITS) profiles of 45 samples collected in different Turkish areas with those of 144 samples from all over Europe. We identified 63 haplotypes, 32 of which were exclusively present in Turkey. The majority of these haplotyes were also population specific. Haplotype network analysis and statistical tests highlighted the presence of a genetic structure and phylogeographic pattern, with three spatially distinct genetic clusters (northeastern Europe, southern Europe, and Turkey), with Turkey representing a diversity hotspot. Based on these results, we hypothesize the presence of glacial refugia for T. aestivum in Turkey, whereas European populations likely experienced a population bottleneck. The possible occurrence of cryptic species among Turkish T. aestivum samples also emerged. Our results are of practical relevance for the marketing of T. aestivum truffles and mycorrhizal seedlings and the preservation of the biodiversity of this species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30676256</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1557-2536</ISSN><JournalIssue CitedMedium="Internet"><Volume>111</Volume><Issue>1</Issue><PubDate><MedlineDate>2019 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Mycologia</Title><ISOAbbreviation>Mycologia</ISOAbbreviation></Journal><ArticleTitle>Ribosomal DNA polymorphisms reveal genetic structure and a phylogeographic pattern in the Burgundy truffle Tuber aestivum Vittad.</ArticleTitle><Pagination><MedlinePgn>26-39</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/00275514.2018.1543508</ELocationID><Abstract><AbstractText>Ectomycorrhizal ascomycetes belonging to the genus Tuber produce edible fruiting bodies known as truffles. Tuber aestivum, in particular, is a fungus appreciated worldwide and has a natural distribution throughout Europe. Most of the molecular studies conducted on this species have been focused on the question as to whether or not T. aestivum and the morphologically similar T. uncinatum are conspecific. Conversely, only a handful of studies have assessed the level and distribution of genetic diversity and occurrence of phylogeographic patterns in this species. Here, we analyzed the genetic diversity of T. aestivum over a wide geographic range, performing an extensive sampling of specimens from Turkey, which is novel, to the best of our knowledge. We compared the internal transcribed spacer (ITS) profiles of 45 samples collected in different Turkish areas with those of 144 samples from all over Europe. We identified 63 haplotypes, 32 of which were exclusively present in Turkey. The majority of these haplotyes were also population specific. Haplotype network analysis and statistical tests highlighted the presence of a genetic structure and phylogeographic pattern, with three spatially distinct genetic clusters (northeastern Europe, southern Europe, and Turkey), with Turkey representing a diversity hotspot. Based on these results, we hypothesize the presence of glacial refugia for T. aestivum in Turkey, whereas European populations likely experienced a population bottleneck. The possible occurrence of cryptic species among Turkish T. aestivum samples also emerged. Our results are of practical relevance for the marketing of T. aestivum truffles and mycorrhizal seedlings and the preservation of the biodiversity of this species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Riccioni</LastName><ForeName>Claudia</ForeName><Initials>C</Initials><Identifier Source="ORCID">0000-0003-1069-8943</Identifier><AffiliationInfo><Affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rubini</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0003-3988-6294</Identifier><AffiliationInfo><Affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Türkoğlu</LastName><ForeName>Aziz</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>b School of Environmental and Forest Sciences, Box 352100 University of Washington , Seattle , Washington 98195-2100.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Belfiori</LastName><ForeName>Beatrice</ForeName><Initials>B</Initials><Identifier Source="ORCID">0000-0002-5366-8579</Identifier><AffiliationInfo><Affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paolocci</LastName><ForeName>Francesco</ForeName><Initials>F</Initials><Identifier Source="ORCID">0000-0002-9394-876X</Identifier><AffiliationInfo><Affiliation>a Institute of Biosciences and Bioresources Perugia Division, National Research Council , Via Madonna Alta n. 130, 06128 Perugia , Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>01</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mycologia</MedlineTA><NlmUniqueID>0400764</NlmUniqueID><ISSNLinking>0027-5514</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021901">DNA, Intergenic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004275">DNA, Ribosomal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021901" MajorTopicYN="N">DNA, Intergenic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004275" MajorTopicYN="N">DNA, Ribosomal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006239" MajorTopicYN="N">Haplotypes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058974" MajorTopicYN="N">Phylogeography</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011110" MajorTopicYN="Y">Polymorphism, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014421" MajorTopicYN="N" Type="Geographic">Turkey</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Ectomycorrhizal fungi</Keyword><Keyword MajorTopicYN="Y">Turkey</Keyword><Keyword MajorTopicYN="Y">genetic pattern</Keyword><Keyword MajorTopicYN="Y">internal transcribed spacer</Keyword><Keyword MajorTopicYN="Y">phylogeography</Keyword><Keyword MajorTopicYN="Y">truffles</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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