Agrobacterium rhizogenes-mediated transformation of Pisum sativum L. roots as a tool for studying the mycorrhizal and root nodule symbioses.
Identifieur interne : 000533 ( Main/Corpus ); précédent : 000532; suivant : 000534Agrobacterium rhizogenes-mediated transformation of Pisum sativum L. roots as a tool for studying the mycorrhizal and root nodule symbioses.
Auteurs : Irina V. Leppyanen ; Anna N. Kirienko ; Elena A. DolgikhSource :
- PeerJ [ 2167-8359 ] ; 2019.
Abstract
In this study, we demonstrated the successful transformation of two pea (Pisum sativum L.) cultivars using Agrobacterium rhizogenes, whereby transgenic roots in the resulting composite plants showed expression of the gene encoding the green fluorescent protein. Subsequent to infection with A. rhizogenes, approximately 70%-80% of pea seedlings developed transgenic hairy roots. We found out that the transgenic roots can be efficiently nodulated by Rhizobium leguminosarum bv. viciae and infected by the arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis. The morphology of nodules in the transgenic roots was found to be identical to that of nodules observed in wild-type roots, and we also observed the effective induction of markers typical of the symbiotic association with AM fungi. The convenient protocol for highly efficient A. rhizogenes-mediated transformation developed in this study would be a rapid and effective tool for investigating those genes involved in the development of the two types of symbioses found in pea plants.
DOI: 10.7717/peerj.6552
PubMed: 30863680
PubMed Central: PMC6408910
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pubmed:30863680Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><i>Agrobacterium rhizogenes</i>
-mediated transformation of <i>Pisum sativum</i>
L. roots as a tool for studying the mycorrhizal and root nodule symbioses.</title>
<author><name sortKey="Leppyanen, Irina V" sort="Leppyanen, Irina V" uniqKey="Leppyanen I" first="Irina V" last="Leppyanen">Irina V. Leppyanen</name>
<affiliation><nlm:affiliation>All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, Russia.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Kirienko, Anna N" sort="Kirienko, Anna N" uniqKey="Kirienko A" first="Anna N" last="Kirienko">Anna N. Kirienko</name>
<affiliation><nlm:affiliation>All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, Russia.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Dolgikh, Elena A" sort="Dolgikh, Elena A" uniqKey="Dolgikh E" first="Elena A" last="Dolgikh">Elena A. Dolgikh</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en"><i>Agrobacterium rhizogenes</i>
-mediated transformation of <i>Pisum sativum</i>
L. roots as a tool for studying the mycorrhizal and root nodule symbioses.</title>
<author><name sortKey="Leppyanen, Irina V" sort="Leppyanen, Irina V" uniqKey="Leppyanen I" first="Irina V" last="Leppyanen">Irina V. Leppyanen</name>
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<author><name sortKey="Kirienko, Anna N" sort="Kirienko, Anna N" uniqKey="Kirienko A" first="Anna N" last="Kirienko">Anna N. Kirienko</name>
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<author><name sortKey="Dolgikh, Elena A" sort="Dolgikh, Elena A" uniqKey="Dolgikh E" first="Elena A" last="Dolgikh">Elena A. Dolgikh</name>
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<front><div type="abstract" xml:lang="en">In this study, we demonstrated the successful transformation of two pea (<i>Pisum sativum</i>
L.) cultivars using <i>Agrobacterium rhizogenes</i>
, whereby transgenic roots in the resulting composite plants showed expression of the gene encoding the green fluorescent protein. Subsequent to infection with <i>A. rhizogenes</i>
, approximately 70%-80% of pea seedlings developed transgenic hairy roots. We found out that the transgenic roots can be efficiently nodulated by <i>Rhizobium leguminosarum</i>
bv. <i>viciae</i>
and infected by the arbuscular mycorrhizal (AM) fungus <i>Rhizophagus irregularis</i>
. The morphology of nodules in the transgenic roots was found to be identical to that of nodules observed in wild-type roots, and we also observed the effective induction of markers typical of the symbiotic association with AM fungi. The convenient protocol for highly efficient <i>A. rhizogenes</i>
-mediated transformation developed in this study would be a rapid and effective tool for investigating those genes involved in the development of the two types of symbioses found in pea plants.</div>
</front>
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<ArticleTitle><i>Agrobacterium rhizogenes</i>
-mediated transformation of <i>Pisum sativum</i>
L. roots as a tool for studying the mycorrhizal and root nodule symbioses.</ArticleTitle>
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<Abstract><AbstractText>In this study, we demonstrated the successful transformation of two pea (<i>Pisum sativum</i>
L.) cultivars using <i>Agrobacterium rhizogenes</i>
, whereby transgenic roots in the resulting composite plants showed expression of the gene encoding the green fluorescent protein. Subsequent to infection with <i>A. rhizogenes</i>
, approximately 70%-80% of pea seedlings developed transgenic hairy roots. We found out that the transgenic roots can be efficiently nodulated by <i>Rhizobium leguminosarum</i>
bv. <i>viciae</i>
and infected by the arbuscular mycorrhizal (AM) fungus <i>Rhizophagus irregularis</i>
. The morphology of nodules in the transgenic roots was found to be identical to that of nodules observed in wild-type roots, and we also observed the effective induction of markers typical of the symbiotic association with AM fungi. The convenient protocol for highly efficient <i>A. rhizogenes</i>
-mediated transformation developed in this study would be a rapid and effective tool for investigating those genes involved in the development of the two types of symbioses found in pea plants.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Leppyanen</LastName>
<ForeName>Irina V</ForeName>
<Initials>IV</Initials>
<AffiliationInfo><Affiliation>All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, Russia.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Kirienko</LastName>
<ForeName>Anna N</ForeName>
<Initials>AN</Initials>
<AffiliationInfo><Affiliation>All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, Russia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dolgikh</LastName>
<ForeName>Elena A</ForeName>
<Initials>EA</Initials>
<AffiliationInfo><Affiliation>All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, Russia.</Affiliation>
</AffiliationInfo>
</Author>
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<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
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<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>03</Month>
<Day>06</Day>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium rhizogenes</Keyword>
<Keyword MajorTopicYN="N">Composite plants</Keyword>
<Keyword MajorTopicYN="N">Mycorrhizae</Keyword>
<Keyword MajorTopicYN="N">Nodulation</Keyword>
<Keyword MajorTopicYN="N">Pea transformation</Keyword>
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<CoiStatement>The authors declare there are no competing interests.</CoiStatement>
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