Rhizophagus intraradices promotes alfalfa (Medicago sativa) defense against pea aphids (Acyrthosiphon pisum) revealed by RNA-Seq analysis.
Identifieur interne : 000303 ( Main/Corpus ); précédent : 000302; suivant : 000304Rhizophagus intraradices promotes alfalfa (Medicago sativa) defense against pea aphids (Acyrthosiphon pisum) revealed by RNA-Seq analysis.
Auteurs : Yingde Li ; Zhibiao Nan ; Tingyu DuanSource :
- Mycorrhiza [ 1432-1890 ] ; 2019.
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Abstract
Pea aphids (Acyrthosiphon pisum) are one of the most important insect pests of alfalfa (Medicago sativa). Arbuscular mycorrhizal (AM) fungi are important microorganisms of the agroecosystem that promote plant growth and improve plant resistance to abiotic and biotic stress. Little information is available on AM fungi-regulated defense responses of alfalfa to pea aphids. To better understand how alfalfa responds and to evaluate the impact of an AM fungus on aphid infestation, transcriptome sequencing was done and physiological parameters were analyzed. Our experiments showed that Rhizophagus intraradices can regulate plant response to aphids by promoting growth and increasing plant peroxidase (POD) and catalase (CAT) activities and salicylic acid (SA) concentration after aphid infestation. Transcriptome analysis showed that R. intraradices increased the expression of resistance-related genes, such as "WRKY transcription factor" and "Kunitz trypsin inhibitor." Additionally, GO terms "chitinase activity," "peroxidase activity," "defense response," and "response to biotic stimulus," and KEGG pathways "phenylpropanoid biosynthesis" and "phenylalanine metabolism" were significantly enriched in mycorrhizal fungus-inoculated plants and aphid-infested plants. These findings will improve our understanding about the impact of this AM fungus on alfalfa response to aphid feeding and will provide the basis for further research on plant defense against aphids.
DOI: 10.1007/s00572-019-00915-0
PubMed: 31588522
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rhizophagus intraradices promotes alfalfa (Medicago sativa) defense against pea aphids (Acyrthosiphon pisum) revealed by RNA-Seq analysis.</title><author><name sortKey="Li, Yingde" sort="Li, Yingde" uniqKey="Li Y" first="Yingde" last="Li">Yingde Li</name><affiliation><nlm:affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China.</nlm:affiliation></affiliation></author><author><name sortKey="Nan, Zhibiao" sort="Nan, Zhibiao" uniqKey="Nan Z" first="Zhibiao" last="Nan">Zhibiao Nan</name><affiliation><nlm:affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China.</nlm:affiliation></affiliation></author><author><name sortKey="Duan, Tingyu" sort="Duan, Tingyu" uniqKey="Duan T" first="Tingyu" last="Duan">Tingyu Duan</name><affiliation><nlm:affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China. duanty@lzu.edu.cnz.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China. duanty@lzu.edu.cnz.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China. duanty@lzu.edu.cnz.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31588522</idno><idno type="pmid">31588522</idno><idno type="doi">10.1007/s00572-019-00915-0</idno><idno type="wicri:Area/Main/Corpus">000303</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000303</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rhizophagus intraradices promotes alfalfa (Medicago sativa) defense against pea aphids (Acyrthosiphon pisum) revealed by RNA-Seq analysis.</title><author><name sortKey="Li, Yingde" sort="Li, Yingde" uniqKey="Li Y" first="Yingde" last="Li">Yingde Li</name><affiliation><nlm:affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China.</nlm:affiliation></affiliation></author><author><name sortKey="Nan, Zhibiao" sort="Nan, Zhibiao" uniqKey="Nan Z" first="Zhibiao" last="Nan">Zhibiao Nan</name><affiliation><nlm:affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China.</nlm:affiliation></affiliation></author><author><name sortKey="Duan, Tingyu" sort="Duan, Tingyu" uniqKey="Duan T" first="Tingyu" last="Duan">Tingyu Duan</name><affiliation><nlm:affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China. duanty@lzu.edu.cnz.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China. duanty@lzu.edu.cnz.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China. duanty@lzu.edu.cnz.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="eISSN">1432-1890</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Aphids (MeSH)</term><term>Medicago sativa (MeSH)</term><term>Mycorrhizae (MeSH)</term><term>Peas (MeSH)</term><term>RNA-Seq (MeSH)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Aphids</term><term>Medicago sativa</term><term>Mycorrhizae</term><term>Peas</term><term>RNA-Seq</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pea aphids (Acyrthosiphon pisum) are one of the most important insect pests of alfalfa (Medicago sativa). Arbuscular mycorrhizal (AM) fungi are important microorganisms of the agroecosystem that promote plant growth and improve plant resistance to abiotic and biotic stress. Little information is available on AM fungi-regulated defense responses of alfalfa to pea aphids. To better understand how alfalfa responds and to evaluate the impact of an AM fungus on aphid infestation, transcriptome sequencing was done and physiological parameters were analyzed. Our experiments showed that Rhizophagus intraradices can regulate plant response to aphids by promoting growth and increasing plant peroxidase (POD) and catalase (CAT) activities and salicylic acid (SA) concentration after aphid infestation. Transcriptome analysis showed that R. intraradices increased the expression of resistance-related genes, such as "WRKY transcription factor" and "Kunitz trypsin inhibitor." Additionally, GO terms "chitinase activity," "peroxidase activity," "defense response," and "response to biotic stimulus," and KEGG pathways "phenylpropanoid biosynthesis" and "phenylalanine metabolism" were significantly enriched in mycorrhizal fungus-inoculated plants and aphid-infested plants. These findings will improve our understanding about the impact of this AM fungus on alfalfa response to aphid feeding and will provide the basis for further research on plant defense against aphids.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">31588522</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>21</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1890</ISSN><JournalIssue CitedMedium="Internet"><Volume>29</Volume><Issue>6</Issue><PubDate><Year>2019</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Rhizophagus intraradices promotes alfalfa (Medicago sativa) defense against pea aphids (Acyrthosiphon pisum) revealed by RNA-Seq analysis.</ArticleTitle><Pagination><MedlinePgn>623-635</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-019-00915-0</ELocationID><Abstract><AbstractText>Pea aphids (Acyrthosiphon pisum) are one of the most important insect pests of alfalfa (Medicago sativa). Arbuscular mycorrhizal (AM) fungi are important microorganisms of the agroecosystem that promote plant growth and improve plant resistance to abiotic and biotic stress. Little information is available on AM fungi-regulated defense responses of alfalfa to pea aphids. To better understand how alfalfa responds and to evaluate the impact of an AM fungus on aphid infestation, transcriptome sequencing was done and physiological parameters were analyzed. Our experiments showed that Rhizophagus intraradices can regulate plant response to aphids by promoting growth and increasing plant peroxidase (POD) and catalase (CAT) activities and salicylic acid (SA) concentration after aphid infestation. Transcriptome analysis showed that R. intraradices increased the expression of resistance-related genes, such as "WRKY transcription factor" and "Kunitz trypsin inhibitor." Additionally, GO terms "chitinase activity," "peroxidase activity," "defense response," and "response to biotic stimulus," and KEGG pathways "phenylpropanoid biosynthesis" and "phenylalanine metabolism" were significantly enriched in mycorrhizal fungus-inoculated plants and aphid-infested plants. These findings will improve our understanding about the impact of this AM fungus on alfalfa response to aphid feeding and will provide the basis for further research on plant defense against aphids.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yingde</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nan</LastName><ForeName>Zhibiao</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Tingyu</ForeName><Initials>T</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-9518-4073</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Grassland Agro-Ecosystems Lanzhou Unviersity, Lanzhou, China. duanty@lzu.edu.cnz.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, China. duanty@lzu.edu.cnz.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Pastoral Agriculture Science and Technology, Lanzhou University, 768 Jiayuguan West Road, Lanzhou, 730020, NO, China. duanty@lzu.edu.cnz.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CARS-22 Green Manure</GrantID><Agency>Agriculture Research System of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001042" MajorTopicYN="Y">Aphids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000455" MajorTopicYN="N">Medicago sativa</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="Y">Mycorrhizae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018532" MajorTopicYN="N">Peas</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000081246" MajorTopicYN="N">RNA-Seq</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Acyrthosiphon pisum</Keyword><Keyword MajorTopicYN="N">Medicago sativa</Keyword><Keyword MajorTopicYN="N">RNA-Seq</Keyword><Keyword MajorTopicYN="N">Rhizophagus intraradices</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>02</Month><Day>09</Day></PubMedPubDate><PubMedPubDate 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