Mycorrhizal networks facilitate the colonization of legume roots by a symbiotic nitrogen-fixing bacterium.
Identifieur interne : 000136 ( Main/Corpus ); précédent : 000135; suivant : 000137Mycorrhizal networks facilitate the colonization of legume roots by a symbiotic nitrogen-fixing bacterium.
Auteurs : Candido Barreto De Novais ; Cristiana Sbrana ; Ederson Da Conceição Jesus ; Luc Felicianus Marie Rouws ; Manuela Giovannetti ; Luciano Avio ; José Oswaldo Siqueira ; Orivaldo José Saggin Júnior ; Eliane Maria Ribeiro Da Silva ; Sergio Miana De FariaSource :
- Mycorrhiza [ 1432-1890 ] ; 2020.
English descriptors
- KwdEn :
- MESH :
- chemical : Nitrogen.
- Bacteria, Fabaceae, Mycorrhizae, Plant Roots, Symbiosis.
Abstract
Arbuscular mycorrhizal fungi (AMF) absorb and translocate nutrients from soil to their host plants by means of a wide network of extraradical mycelium (ERM). Here, we assessed whether nitrogen-fixing rhizobia can be transferred to the host legume Glycine max by ERM produced by Glomus formosanum isolate CNPAB020 colonizing the grass Urochloa decumbens. An H-bridge experimental system was developed to evaluate the migration of ERM and of the GFP-tagged Bradyrhizobium diazoefficiens USDA 110 strain across an air gap compartment. Mycorrhizal colonization, nodule formation in legumes, and occurrence of the GFP-tagged strain in root nodules were assessed by optical and confocal laser scanning microscopy. In the presence of non-mycorrhizal U. decumbens, legume roots were neither AMF-colonized nor nodulated. In contrast, G. formosanum ERM crossing the discontinuous compartment connected mycorrhizal U. decumbens and G. max roots, which showed 30-42% mycorrhizal colonization and 7-11 nodules per plant. Fluorescent B. diazoefficiens cells were detected in 94% of G. max root nodules. Our findings reveal that, besides its main activity in nutrient transfer, ERM produced by AMF may facilitate bacterial translocation and the simultaneous associations of plants with beneficial fungi and bacteria, representing an important structure, functional to the establishment of symbiotic relationships.
DOI: 10.1007/s00572-020-00948-w
PubMed: 32215759
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pubmed:32215759Le document en format XML
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the colonization of legume roots by a symbiotic nitrogen-fixing bacterium.</title><author><name sortKey="De Novais, Candido Barreto" sort="De Novais, Candido Barreto" uniqKey="De Novais C" first="Candido Barreto" last="De Novais">Candido Barreto De Novais</name><affiliation><nlm:affiliation>Instituto de Floresta, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 23890-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Sbrana, Cristiana" sort="Sbrana, Cristiana" uniqKey="Sbrana C" first="Cristiana" last="Sbrana">Cristiana Sbrana</name><affiliation><nlm:affiliation>CNR-Institute of Agricultural Biology and Biotechnology UOS Pisa, Pisa, Italy. cristiana.sbrana@ibba.cnr.it.</nlm:affiliation></affiliation></author><author><name sortKey="Da Conceicao Jesus, Ederson" sort="Da Conceicao Jesus, Ederson" uniqKey="Da Conceicao Jesus E" first="Ederson" last="Da Conceição Jesus">Ederson Da Conceição Jesus</name><affiliation><nlm:affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Rouws, Luc Felicianus Marie" sort="Rouws, Luc Felicianus Marie" uniqKey="Rouws L" first="Luc Felicianus Marie" last="Rouws">Luc Felicianus Marie Rouws</name><affiliation><nlm:affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Giovannetti, Manuela" sort="Giovannetti, Manuela" uniqKey="Giovannetti M" first="Manuela" last="Giovannetti">Manuela Giovannetti</name><affiliation><nlm:affiliation>Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Avio, Luciano" sort="Avio, Luciano" uniqKey="Avio L" first="Luciano" last="Avio">Luciano Avio</name><affiliation><nlm:affiliation>Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Siqueira, Jose Oswaldo" sort="Siqueira, Jose Oswaldo" uniqKey="Siqueira J" first="José Oswaldo" last="Siqueira">José Oswaldo Siqueira</name><affiliation><nlm:affiliation>DCS-Laboratório de Microbiologia do Solo, Universidade Federal de Lavras, Caixa Postal 3037, Lavras, MG, 37200-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Saggin Junior, Orivaldo Jose" sort="Saggin Junior, Orivaldo Jose" uniqKey="Saggin Junior O" first="Orivaldo José" last="Saggin Júnior">Orivaldo José Saggin Júnior</name><affiliation><nlm:affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Da Silva, Eliane Maria Ribeiro" sort="Da Silva, Eliane Maria Ribeiro" uniqKey="Da Silva E" first="Eliane Maria Ribeiro" last="Da Silva">Eliane Maria Ribeiro Da Silva</name><affiliation><nlm:affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="De Faria, Sergio Miana" sort="De Faria, Sergio Miana" uniqKey="De Faria S" first="Sergio Miana" last="De Faria">Sergio Miana De Faria</name><affiliation><nlm:affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="eISSN">1432-1890</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (MeSH)</term><term>Fabaceae (MeSH)</term><term>Mycorrhizae (MeSH)</term><term>Nitrogen (MeSH)</term><term>Plant Roots (MeSH)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Nitrogen</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacteria</term><term>Fabaceae</term><term>Mycorrhizae</term><term>Plant Roots</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arbuscular mycorrhizal fungi (AMF) absorb and translocate nutrients from soil to their host plants by means of a wide network of extraradical mycelium (ERM). Here, we assessed whether nitrogen-fixing rhizobia can be transferred to the host legume Glycine max by ERM produced by Glomus formosanum isolate CNPAB020 colonizing the grass Urochloa decumbens. An H-bridge experimental system was developed to evaluate the migration of ERM and of the GFP-tagged Bradyrhizobium diazoefficiens USDA 110 strain across an air gap compartment. Mycorrhizal colonization, nodule formation in legumes, and occurrence of the GFP-tagged strain in root nodules were assessed by optical and confocal laser scanning microscopy. In the presence of non-mycorrhizal U. decumbens, legume roots were neither AMF-colonized nor nodulated. In contrast, G. formosanum ERM crossing the discontinuous compartment connected mycorrhizal U. decumbens and G. max roots, which showed 30-42% mycorrhizal colonization and 7-11 nodules per plant. Fluorescent B. diazoefficiens cells were detected in 94% of G. max root nodules. Our findings reveal that, besides its main activity in nutrient transfer, ERM produced by AMF may facilitate bacterial translocation and the simultaneous associations of plants with beneficial fungi and bacteria, representing an important structure, functional to the establishment of symbiotic relationships.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">32215759</PMID><DateCompleted><Year>2020</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2020</Year><Month>05</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1890</ISSN><JournalIssue CitedMedium="Internet"><Volume>30</Volume><Issue>2-3</Issue><PubDate><Year>2020</Year><Month>May</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Mycorrhizal networks facilitate the colonization of legume roots by a symbiotic nitrogen-fixing bacterium.</ArticleTitle><Pagination><MedlinePgn>389-396</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-020-00948-w</ELocationID><Abstract><AbstractText>Arbuscular mycorrhizal fungi (AMF) absorb and translocate nutrients from soil to their host plants by means of a wide network of extraradical mycelium (ERM). Here, we assessed whether nitrogen-fixing rhizobia can be transferred to the host legume Glycine max by ERM produced by Glomus formosanum isolate CNPAB020 colonizing the grass Urochloa decumbens. An H-bridge experimental system was developed to evaluate the migration of ERM and of the GFP-tagged Bradyrhizobium diazoefficiens USDA 110 strain across an air gap compartment. Mycorrhizal colonization, nodule formation in legumes, and occurrence of the GFP-tagged strain in root nodules were assessed by optical and confocal laser scanning microscopy. In the presence of non-mycorrhizal U. decumbens, legume roots were neither AMF-colonized nor nodulated. In contrast, G. formosanum ERM crossing the discontinuous compartment connected mycorrhizal U. decumbens and G. max roots, which showed 30-42% mycorrhizal colonization and 7-11 nodules per plant. Fluorescent B. diazoefficiens cells were detected in 94% of G. max root nodules. Our findings reveal that, besides its main activity in nutrient transfer, ERM produced by AMF may facilitate bacterial translocation and the simultaneous associations of plants with beneficial fungi and bacteria, representing an important structure, functional to the establishment of symbiotic relationships.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>de Novais</LastName><ForeName>Candido Barreto</ForeName><Initials>CB</Initials><AffiliationInfo><Affiliation>Instituto de Floresta, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 23890-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sbrana</LastName><ForeName>Cristiana</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>CNR-Institute of Agricultural Biology and Biotechnology UOS Pisa, Pisa, Italy. cristiana.sbrana@ibba.cnr.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>da Conceição Jesus</LastName><ForeName>Ederson</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rouws</LastName><ForeName>Luc Felicianus Marie</ForeName><Initials>LFM</Initials><AffiliationInfo><Affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Giovannetti</LastName><ForeName>Manuela</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Avio</LastName><ForeName>Luciano</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Siqueira</LastName><ForeName>José Oswaldo</ForeName><Initials>JO</Initials><AffiliationInfo><Affiliation>DCS-Laboratório de Microbiologia do Solo, Universidade Federal de Lavras, Caixa Postal 3037, Lavras, MG, 37200-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saggin Júnior</LastName><ForeName>Orivaldo José</ForeName><Initials>OJ</Initials><AffiliationInfo><Affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>da Silva</LastName><ForeName>Eliane Maria Ribeiro</ForeName><Initials>EMR</Initials><AffiliationInfo><Affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Faria</LastName><ForeName>Sergio Miana</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23891-000, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>none</GrantID><Agency>CAPES</Agency><Country></Country></Grant><Grant><GrantID>311436/2017-0, 307872/2016-5 and E-26/010.001869/2017</GrantID><Agency>National Council for Scientific and Technological Development</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007887" MajorTopicYN="Y">Fabaceae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="Y">Mycorrhizae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arbuscular mycorrhizal fungi</Keyword><Keyword MajorTopicYN="N">Bacterial migration</Keyword><Keyword MajorTopicYN="N">Common mycorrhizal network</Keyword><Keyword MajorTopicYN="N">Extraradical mycelium</Keyword><Keyword MajorTopicYN="N">Plant symbioses</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32215759</ArticleId><ArticleId IdType="doi">10.1007/s00572-020-00948-w</ArticleId><ArticleId IdType="pii">10.1007/s00572-020-00948-w</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour 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