Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae.
Identifieur interne : 000099 ( Main/Corpus ); précédent : 000098; suivant : 000100Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae.
Auteurs : Ghislaine Recorbet ; Pierre-Emmanuel Courty ; Daniel WipfSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2020.
Abstract
In arbuscular mycorrhizal symbiosis, the belowground mycelium that develops into the soil, not only provides extensive pathways for nutrient fluxes, the occupation of different niches, and dispersal of propagules, but also has strong influences upon biogeochemical cycling. By providing a valuable overview of expression changes of most proteins, shotgun proteomics can help decipher key metabolic pathways involved in the functioning of fungal mycelia. In this protocol, we describe the combination of extra-radical mycelium growth systems with gel-based extraction of fungal peptides amenable for shotgun protein profiling, which allows gaining information about the extra-radical proteome.
DOI: 10.1007/978-1-0716-0603-2_17
PubMed: 32415607
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By providing a valuable overview of expression changes of most proteins, shotgun proteomics can help decipher key metabolic pathways involved in the functioning of fungal mycelia. In this protocol, we describe the combination of extra-radical mycelium growth systems with gel-based extraction of fungal peptides amenable for shotgun protein profiling, which allows gaining information about the extra-radical proteome.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32415607</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>2146</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae.</ArticleTitle><Pagination><MedlinePgn>223-238</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-0716-0603-2_17</ELocationID><Abstract><AbstractText>In arbuscular mycorrhizal symbiosis, the belowground mycelium that develops into the soil, not only provides extensive pathways for nutrient fluxes, the occupation of different niches, and dispersal of propagules, but also has strong influences upon biogeochemical cycling. By providing a valuable overview of expression changes of most proteins, shotgun proteomics can help decipher key metabolic pathways involved in the functioning of fungal mycelia. In this protocol, we describe the combination of extra-radical mycelium growth systems with gel-based extraction of fungal peptides amenable for shotgun protein profiling, which allows gaining information about the extra-radical proteome.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Recorbet</LastName><ForeName>Ghislaine</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Agroécologie, AgroSup Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté, Dijon, France. ghislaine.recorbet@inra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Courty</LastName><ForeName>Pierre-Emmanuel</ForeName><Initials>PE</Initials><AffiliationInfo><Affiliation>Agroécologie, AgroSup Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté, Dijon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wipf</LastName><ForeName>Daniel</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Agroécologie, AgroSup Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté, Dijon, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Bi-compartmented soil microcosm</Keyword><Keyword MajorTopicYN="Y">Extra-radical mycelium</Keyword><Keyword MajorTopicYN="Y">In-gel digestion</Keyword><Keyword MajorTopicYN="Y">Proteomics</Keyword><Keyword MajorTopicYN="Y">Root organ culture</Keyword><Keyword MajorTopicYN="Y">SDS-PAGE</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32415607</ArticleId><ArticleId IdType="doi">10.1007/978-1-0716-0603-2_17</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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