Role of the Fusarium oxysporum metallothionein Mt1 in resistance to metal toxicity and virulence.
Identifieur interne : 000030 ( Main/Corpus ); précédent : 000029; suivant : 000031Role of the Fusarium oxysporum metallothionein Mt1 in resistance to metal toxicity and virulence.
Auteurs : Damaris Lorenzo-Gutiérrez ; Lucía G Mez-Gil ; Josep Guarro ; M Isabel G. Roncero ; Ana Fernández-Bravo ; Javier Capilla ; Loida L Pez-FernándezSource :
- Metallomics : integrated biometal science [ 1756-591X ] ; 2019.
English descriptors
- KwdEn :
- Animals (MeSH), Cadmium (metabolism), Cell Line (MeSH), Copper (metabolism), Fungal Proteins (metabolism), Fusariosis (metabolism), Fusariosis (microbiology), Fusariosis (pathology), Fusarium (genetics), Fusarium (metabolism), Fusarium (pathogenicity), Gene Deletion (MeSH), Lycopersicon esculentum (microbiology), Male (MeSH), Metallothionein (genetics), Metallothionein (metabolism), Metals, Heavy (metabolism), Mice (MeSH), Plant Diseases (microbiology), Virulence (MeSH), Zinc (metabolism).
- MESH :
- chemical , genetics : Metallothionein.
- chemical , metabolism : Cadmium, Copper, Fungal Proteins, Metallothionein, Metals, Heavy, Zinc.
- genetics : Fusarium.
- metabolism : Fusariosis, Fusarium.
- microbiology : Fusariosis, Lycopersicon esculentum, Plant Diseases.
- pathogenicity : Fusarium.
- pathology : Fusariosis.
- Animals, Cell Line, Gene Deletion, Male, Mice, Virulence.
Abstract
Soil organisms exhibit high tolerance to heavy metals, probably acquired through evolutionary adaptation to contaminated environments. Essentially, metal tolerance in fungi involves several specific and non-specific mechanisms that include metal efflux, metal binding to cell walls, extracellular and intracellular sequestration and complexation with proteins. However, fungi have adopted different strategies to detoxify heavy metals, although species differ in the mechanisms used. In this complex molecular framework, metallothioneins (MTs) are becoming increasingly relevant in metal homeostasis, even though little is known about their role in metal adaptation and virulence in fungal pathogens. With the aim to decipher the function of metallothioneins in the opportunistic fungus Fusarium oxysporum, we have carried out an in silico analysis that revealed the presence of a hypothetical metallothionein (mt1) that has multiple metal responsive elements in its promoter region and conserved cysteine motifs in its coding sequence. Characterization of strain Δmt1 deficient in the mt1 gene revealed higher sensitivity of this mutant to copper, cadmium and zinc compared to the wild type strain (wt). Expression analyses revealed that Zn specifically activates mt1, but the lack of this gene did not lead to a transcriptional up-regulation of genes gapdh and prx, associated with the oxidative stress response. The lack of mt1 did not alter the pathogenic capacity of the fungus, either in tomato plant or in a murine model of systemic infection. Nevertheless, Δmt1 displayed lower resistance to macrophage killing, suggesting a connection between the absence of mt1 and impaired defence capacity against copper and reactive oxygen species.
DOI: 10.1039/c9mt00081j
PubMed: 31134239
Links to Exploration step
pubmed:31134239Le document en format XML
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Roncero</name><affiliation><nlm:affiliation>Departamento de Genetica, Facultad de Ciencias and Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Cordoba, 14071 Cordoba, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Fernandez Bravo, Ana" sort="Fernandez Bravo, Ana" uniqKey="Fernandez Bravo A" first="Ana" last="Fernández-Bravo">Ana Fernández-Bravo</name><affiliation><nlm:affiliation>Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut and Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Reus, Spain. loidalopezfernandez@gmail.com javier.capilla@urv.cat.</nlm:affiliation></affiliation></author><author><name sortKey="Capilla, Javier" sort="Capilla, Javier" uniqKey="Capilla J" first="Javier" last="Capilla">Javier Capilla</name><affiliation><nlm:affiliation>Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut and Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Reus, Spain. loidalopezfernandez@gmail.com javier.capilla@urv.cat.</nlm:affiliation></affiliation></author><author><name sortKey="L Pez Fernandez, Loida" sort="L Pez Fernandez, Loida" uniqKey="L Pez Fernandez L" first="Loida" last="L Pez-Fernández">Loida L Pez-Fernández</name><affiliation><nlm:affiliation>Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut and Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Reus, Spain. loidalopezfernandez@gmail.com javier.capilla@urv.cat.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Metallomics : integrated biometal science</title><idno type="eISSN">1756-591X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Cadmium (metabolism)</term><term>Cell Line (MeSH)</term><term>Copper (metabolism)</term><term>Fungal Proteins (metabolism)</term><term>Fusariosis (metabolism)</term><term>Fusariosis (microbiology)</term><term>Fusariosis (pathology)</term><term>Fusarium (genetics)</term><term>Fusarium (metabolism)</term><term>Fusarium (pathogenicity)</term><term>Gene Deletion (MeSH)</term><term>Lycopersicon esculentum (microbiology)</term><term>Male (MeSH)</term><term>Metallothionein (genetics)</term><term>Metallothionein (metabolism)</term><term>Metals, Heavy (metabolism)</term><term>Mice (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Virulence (MeSH)</term><term>Zinc (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Metallothionein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cadmium</term><term>Copper</term><term>Fungal Proteins</term><term>Metallothionein</term><term>Metals, Heavy</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fusariosis</term><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Fusariosis</term><term>Lycopersicon esculentum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Fusariosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Gene Deletion</term><term>Male</term><term>Mice</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Soil organisms exhibit high tolerance to heavy metals, probably acquired through evolutionary adaptation to contaminated environments. Essentially, metal tolerance in fungi involves several specific and non-specific mechanisms that include metal efflux, metal binding to cell walls, extracellular and intracellular sequestration and complexation with proteins. However, fungi have adopted different strategies to detoxify heavy metals, although species differ in the mechanisms used. In this complex molecular framework, metallothioneins (MTs) are becoming increasingly relevant in metal homeostasis, even though little is known about their role in metal adaptation and virulence in fungal pathogens. With the aim to decipher the function of metallothioneins in the opportunistic fungus Fusarium oxysporum, we have carried out an in silico analysis that revealed the presence of a hypothetical metallothionein (mt1) that has multiple metal responsive elements in its promoter region and conserved cysteine motifs in its coding sequence. Characterization of strain Δmt1 deficient in the mt1 gene revealed higher sensitivity of this mutant to copper, cadmium and zinc compared to the wild type strain (wt). Expression analyses revealed that Zn specifically activates mt1, but the lack of this gene did not lead to a transcriptional up-regulation of genes gapdh and prx, associated with the oxidative stress response. The lack of mt1 did not alter the pathogenic capacity of the fungus, either in tomato plant or in a murine model of systemic infection. Nevertheless, Δmt1 displayed lower resistance to macrophage killing, suggesting a connection between the absence of mt1 and impaired defence capacity against copper and reactive oxygen species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31134239</PMID><DateCompleted><Year>2020</Year><Month>05</Month><Day>28</Day></DateCompleted><DateRevised><Year>2020</Year><Month>05</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1756-591X</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>7</Issue><PubDate><Year>2019</Year><Month>07</Month><Day>17</Day></PubDate></JournalIssue><Title>Metallomics : integrated biometal science</Title><ISOAbbreviation>Metallomics</ISOAbbreviation></Journal><ArticleTitle>Role of the Fusarium oxysporum metallothionein Mt1 in resistance to metal toxicity and virulence.</ArticleTitle><Pagination><MedlinePgn>1230-1240</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c9mt00081j</ELocationID><Abstract><AbstractText>Soil organisms exhibit high tolerance to heavy metals, probably acquired through evolutionary adaptation to contaminated environments. Essentially, metal tolerance in fungi involves several specific and non-specific mechanisms that include metal efflux, metal binding to cell walls, extracellular and intracellular sequestration and complexation with proteins. However, fungi have adopted different strategies to detoxify heavy metals, although species differ in the mechanisms used. In this complex molecular framework, metallothioneins (MTs) are becoming increasingly relevant in metal homeostasis, even though little is known about their role in metal adaptation and virulence in fungal pathogens. With the aim to decipher the function of metallothioneins in the opportunistic fungus Fusarium oxysporum, we have carried out an in silico analysis that revealed the presence of a hypothetical metallothionein (mt1) that has multiple metal responsive elements in its promoter region and conserved cysteine motifs in its coding sequence. Characterization of strain Δmt1 deficient in the mt1 gene revealed higher sensitivity of this mutant to copper, cadmium and zinc compared to the wild type strain (wt). Expression analyses revealed that Zn specifically activates mt1, but the lack of this gene did not lead to a transcriptional up-regulation of genes gapdh and prx, associated with the oxidative stress response. The lack of mt1 did not alter the pathogenic capacity of the fungus, either in tomato plant or in a murine model of systemic infection. Nevertheless, Δmt1 displayed lower resistance to macrophage killing, suggesting a connection between the absence of mt1 and impaired defence capacity against copper and reactive oxygen species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lorenzo-Gutiérrez</LastName><ForeName>Damaris</ForeName><Initials>D</Initials><Identifier Source="ORCID">0000-0003-2867-3664</Identifier><AffiliationInfo><Affiliation>Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut and Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Reus, Spain. loidalopezfernandez@gmail.com javier.capilla@urv.cat.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gómez-Gil</LastName><ForeName>Lucía</ForeName><Initials>L</Initials><Identifier Source="ORCID">0000-0001-9512-656X</Identifier><AffiliationInfo><Affiliation>Departamento de Genetica, Facultad de Ciencias and Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Cordoba, 14071 Cordoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guarro</LastName><ForeName>Josep</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-7839-7568</Identifier><AffiliationInfo><Affiliation>Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut and Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Reus, Spain. loidalopezfernandez@gmail.com javier.capilla@urv.cat.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roncero</LastName><ForeName>M Isabel G</ForeName><Initials>MIG</Initials><Identifier Source="ORCID">0000-0002-5283-7836</Identifier><AffiliationInfo><Affiliation>Departamento de Genetica, Facultad de Ciencias and Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Cordoba, 14071 Cordoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernández-Bravo</LastName><ForeName>Ana</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0001-7637-5958</Identifier><AffiliationInfo><Affiliation>Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut and Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Reus, Spain. loidalopezfernandez@gmail.com javier.capilla@urv.cat.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Capilla</LastName><ForeName>Javier</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-0765-6403</Identifier><AffiliationInfo><Affiliation>Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut and Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Reus, Spain. loidalopezfernandez@gmail.com javier.capilla@urv.cat.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>López-Fernández</LastName><ForeName>Loida</ForeName><Initials>L</Initials><Identifier Source="ORCID">0000-0002-6270-9301</Identifier><AffiliationInfo><Affiliation>Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut and Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Reus, Spain. loidalopezfernandez@gmail.com javier.capilla@urv.cat.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Metallomics</MedlineTA><NlmUniqueID>101478346</NlmUniqueID><ISSNLinking>1756-5901</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>00BH33GNGH</RegistryNumber><NameOfSubstance UI="D002104">Cadmium</NameOfSubstance></Chemical><Chemical><RegistryNumber>789U1901C5</RegistryNumber><NameOfSubstance UI="D003300">Copper</NameOfSubstance></Chemical><Chemical><RegistryNumber>9038-94-2</RegistryNumber><NameOfSubstance UI="D008668">Metallothionein</NameOfSubstance></Chemical><Chemical><RegistryNumber>J41CSQ7QDS</RegistryNumber><NameOfSubstance UI="D015032">Zinc</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002104" MajorTopicYN="N">Cadmium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003300" 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Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008668" MajorTopicYN="N">Metallothionein</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" 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