Comparative analysis of secreted protein evolution using expressed sequence tags from four poplar leaf rusts (Melampsora spp.).
Identifieur interne : 000115 ( PubMed/Corpus ); précédent : 000114; suivant : 000116Comparative analysis of secreted protein evolution using expressed sequence tags from four poplar leaf rusts (Melampsora spp.).
Auteurs : David L. Joly ; Nicolas Feau ; Philippe Tanguay ; Richard C. HamelinSource :
- BMC genomics [ 1471-2164 ] ; 2010.
English descriptors
- KwdEn :
- Adaptation, Physiological (MeSH), Basidiomycota (genetics), Basidiomycota (metabolism), Basidiomycota (physiology), Cloning, Molecular (MeSH), Evolution, Molecular (MeSH), Expressed Sequence Tags (metabolism), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Profiling (MeSH), Gene Library (MeSH), Genomics (MeSH), Immunity, Innate (MeSH), Plant Diseases (immunology), Plant Diseases (microbiology), Plant Leaves (microbiology), Proteome (genetics), Proteome (metabolism), Sequence Homology, Nucleic Acid (MeSH).
- MESH :
- chemical , genetics : Fungal Proteins, Proteome.
- genetics : Basidiomycota.
- immunology : Plant Diseases.
- metabolism : Basidiomycota, Expressed Sequence Tags, Fungal Proteins, Proteome.
- microbiology : Plant Diseases, Plant Leaves.
- physiology : Basidiomycota.
- Adaptation, Physiological, Cloning, Molecular, Evolution, Molecular, Gene Expression Profiling, Gene Library, Genomics, Immunity, Innate, Sequence Homology, Nucleic Acid.
Abstract
BACKGROUND
Obligate biotrophs such as rust fungi are believed to establish long-term relationships by modulating plant defenses through a plethora of effector proteins, whose most recognizable feature is the presence of a signal peptide for secretion. Since the phenotypes of these effectors extend to host cells, their genes are expected to be under accelerated evolution stimulated by host-pathogen coevolutionary arms races. Recently, whole genome sequence data has allowed the prediction of secretomes, facilitating the identification of putative effectors.
RESULTS
We generated cDNA libraries from four poplar leaf rust pathogens (Melampsora spp.) and used computational approaches to identify and annotate putative secreted proteins with the aim of uncovering new knowledge about the nature and evolution of the rust secretome. While more than half of the predicted secretome members encoded lineage-specific proteins, similarities with experimentally characterized fungal effectors were also identified. A SAGE analysis indicated a strong stage-specific regulation of transcripts encoding secreted proteins. The average sequence identity of putative secreted proteins to their closest orthologs in the wheat stem rust Puccinia graminis f. sp. tritici was dramatically reduced compared with non-secreted ones. A comparative genomics approach based on homologous gene groups unravelled positive selection in putative members of the secretome.
CONCLUSION
We uncovered robust evidence that different evolutionary constraints are acting on the rust secretome when compared to the rest of the genome. These results are consistent with the view that these genes are more likely to exhibit an effector activity and be involved in coevolutionary arms races with host factors.
DOI: 10.1186/1471-2164-11-422
PubMed: 20615251
PubMed Central: PMC2996950
Links to Exploration step
pubmed:20615251Le document en format XML
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<term>Basidiomycota (physiology)</term>
<term>Cloning, Molecular (MeSH)</term>
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<term>Fungal Proteins (metabolism)</term>
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<term>Plant Diseases (microbiology)</term>
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<term>Proteome (genetics)</term>
<term>Proteome (metabolism)</term>
<term>Sequence Homology, Nucleic Acid (MeSH)</term>
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<term>Evolution, Molecular</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Obligate biotrophs such as rust fungi are believed to establish long-term relationships by modulating plant defenses through a plethora of effector proteins, whose most recognizable feature is the presence of a signal peptide for secretion. Since the phenotypes of these effectors extend to host cells, their genes are expected to be under accelerated evolution stimulated by host-pathogen coevolutionary arms races. Recently, whole genome sequence data has allowed the prediction of secretomes, facilitating the identification of putative effectors.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>We generated cDNA libraries from four poplar leaf rust pathogens (Melampsora spp.) and used computational approaches to identify and annotate putative secreted proteins with the aim of uncovering new knowledge about the nature and evolution of the rust secretome. While more than half of the predicted secretome members encoded lineage-specific proteins, similarities with experimentally characterized fungal effectors were also identified. A SAGE analysis indicated a strong stage-specific regulation of transcripts encoding secreted proteins. The average sequence identity of putative secreted proteins to their closest orthologs in the wheat stem rust Puccinia graminis f. sp. tritici was dramatically reduced compared with non-secreted ones. A comparative genomics approach based on homologous gene groups unravelled positive selection in putative members of the secretome.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSION</b>
</p>
<p>We uncovered robust evidence that different evolutionary constraints are acting on the rust secretome when compared to the rest of the genome. These results are consistent with the view that these genes are more likely to exhibit an effector activity and be involved in coevolutionary arms races with host factors.</p>
</div>
</front>
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<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Obligate biotrophs such as rust fungi are believed to establish long-term relationships by modulating plant defenses through a plethora of effector proteins, whose most recognizable feature is the presence of a signal peptide for secretion. Since the phenotypes of these effectors extend to host cells, their genes are expected to be under accelerated evolution stimulated by host-pathogen coevolutionary arms races. Recently, whole genome sequence data has allowed the prediction of secretomes, facilitating the identification of putative effectors.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">We generated cDNA libraries from four poplar leaf rust pathogens (Melampsora spp.) and used computational approaches to identify and annotate putative secreted proteins with the aim of uncovering new knowledge about the nature and evolution of the rust secretome. While more than half of the predicted secretome members encoded lineage-specific proteins, similarities with experimentally characterized fungal effectors were also identified. A SAGE analysis indicated a strong stage-specific regulation of transcripts encoding secreted proteins. The average sequence identity of putative secreted proteins to their closest orthologs in the wheat stem rust Puccinia graminis f. sp. tritici was dramatically reduced compared with non-secreted ones. A comparative genomics approach based on homologous gene groups unravelled positive selection in putative members of the secretome.</AbstractText>
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