Laser capture microdissection of uredinia formed by Melampsora larici-populina revealed a transcriptional switch between biotrophy and sporulation.
Identifieur interne : 000114 ( PubMed/Corpus ); précédent : 000113; suivant : 000115Laser capture microdissection of uredinia formed by Melampsora larici-populina revealed a transcriptional switch between biotrophy and sporulation.
Auteurs : Stéphane Hacquard ; Christine Delaruelle ; Valérie Legué ; Emilie Tisserant ; Annegret Kohler ; Pascal Frey ; Francis Martin ; Sébastien DuplessisSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2010.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Fungal Proteins.
- chemical , metabolism : Fungal Proteins.
- methods : Microdissection.
- microbiology : Populus.
- physiology : Basidiomycota, Gene Expression Regulation, Bacterial.
- Gene Expression Profiling, Spores, Bacterial, Transcription, Genetic.
Abstract
The foliar rust caused by the basidiomycete Melampsora larici-populina is the main disease affecting poplar plantations in Europe. The biotrophic status of rust fungi is a major limitation to study gene expression of cell or tissue types during host infection. At the uredinial stage, infected poplar leaves contain distinct rust tissues such as haustoria, infection hyphae, and uredinia with sporogenous hyphae and newly formed asexual urediniospores. Laser capture microdissection (LCM) was used to isolate three areas corresponding to uredinia and subjacent zones in the host mesophyll for expression analysis with M. larici-populina whole-genome exon oligoarrays. Optimization of tissue preparation prior to LCM allowed isolation of RNA of good integrity for genome-wide expression profiling. Our results indicate that the poplar rust uredinial stage is marked by distinct genetic programs related to biotrophy in the host palisade mesophyll and to sporulation in the uredinium. A strong induction of transcripts encoding small secreted proteins, likely containing rust effectors, is observed in the mesophyll, suggesting a late maintenance of suppression of host defense in the tissue containing haustoria and infection hyphae. On the other hand, cell cycle and cell defense rescue transcripts are strongly accumulated in the sporulation area. This combined LCM-transcriptomic approach brings new insights on the molecular mechanisms underlying urediniospore formation in rust fungi.
DOI: 10.1094/MPMI-05-10-0111
PubMed: 20831407
Links to Exploration step
pubmed:20831407Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Laser capture microdissection of uredinia formed by Melampsora larici-populina revealed a transcriptional switch between biotrophy and sporulation.</title>
<author><name sortKey="Hacquard, Stephane" sort="Hacquard, Stephane" uniqKey="Hacquard S" first="Stéphane" last="Hacquard">Stéphane Hacquard</name>
<affiliation><nlm:affiliation>Unité Mixte de Recherche 1136 INRA/Nancy Université Interactions Arbres/Micro-organismes, Champenoux, France.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Delaruelle, Christine" sort="Delaruelle, Christine" uniqKey="Delaruelle C" first="Christine" last="Delaruelle">Christine Delaruelle</name>
</author>
<author><name sortKey="Legue, Valerie" sort="Legue, Valerie" uniqKey="Legue V" first="Valérie" last="Legué">Valérie Legué</name>
</author>
<author><name sortKey="Tisserant, Emilie" sort="Tisserant, Emilie" uniqKey="Tisserant E" first="Emilie" last="Tisserant">Emilie Tisserant</name>
</author>
<author><name sortKey="Kohler, Annegret" sort="Kohler, Annegret" uniqKey="Kohler A" first="Annegret" last="Kohler">Annegret Kohler</name>
</author>
<author><name sortKey="Frey, Pascal" sort="Frey, Pascal" uniqKey="Frey P" first="Pascal" last="Frey">Pascal Frey</name>
</author>
<author><name sortKey="Martin, Francis" sort="Martin, Francis" uniqKey="Martin F" first="Francis" last="Martin">Francis Martin</name>
</author>
<author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:20831407</idno>
<idno type="pmid">20831407</idno>
<idno type="doi">10.1094/MPMI-05-10-0111</idno>
<idno type="wicri:Area/PubMed/Corpus">000114</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000114</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Laser capture microdissection of uredinia formed by Melampsora larici-populina revealed a transcriptional switch between biotrophy and sporulation.</title>
<author><name sortKey="Hacquard, Stephane" sort="Hacquard, Stephane" uniqKey="Hacquard S" first="Stéphane" last="Hacquard">Stéphane Hacquard</name>
<affiliation><nlm:affiliation>Unité Mixte de Recherche 1136 INRA/Nancy Université Interactions Arbres/Micro-organismes, Champenoux, France.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Delaruelle, Christine" sort="Delaruelle, Christine" uniqKey="Delaruelle C" first="Christine" last="Delaruelle">Christine Delaruelle</name>
</author>
<author><name sortKey="Legue, Valerie" sort="Legue, Valerie" uniqKey="Legue V" first="Valérie" last="Legué">Valérie Legué</name>
</author>
<author><name sortKey="Tisserant, Emilie" sort="Tisserant, Emilie" uniqKey="Tisserant E" first="Emilie" last="Tisserant">Emilie Tisserant</name>
</author>
<author><name sortKey="Kohler, Annegret" sort="Kohler, Annegret" uniqKey="Kohler A" first="Annegret" last="Kohler">Annegret Kohler</name>
</author>
<author><name sortKey="Frey, Pascal" sort="Frey, Pascal" uniqKey="Frey P" first="Pascal" last="Frey">Pascal Frey</name>
</author>
<author><name sortKey="Martin, Francis" sort="Martin, Francis" uniqKey="Martin F" first="Francis" last="Martin">Francis Martin</name>
</author>
<author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name>
</author>
</analytic>
<series><title level="j">Molecular plant-microbe interactions : MPMI</title>
<idno type="ISSN">0894-0282</idno>
<imprint><date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (physiology)</term>
<term>Fungal Proteins (genetics)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Bacterial (physiology)</term>
<term>Microdissection (methods)</term>
<term>Populus (microbiology)</term>
<term>Spores, Bacterial (MeSH)</term>
<term>Transcription, Genetic (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microdissection</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term>
<term>Gene Expression Regulation, Bacterial</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term>
<term>Spores, Bacterial</term>
<term>Transcription, Genetic</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The foliar rust caused by the basidiomycete Melampsora larici-populina is the main disease affecting poplar plantations in Europe. The biotrophic status of rust fungi is a major limitation to study gene expression of cell or tissue types during host infection. At the uredinial stage, infected poplar leaves contain distinct rust tissues such as haustoria, infection hyphae, and uredinia with sporogenous hyphae and newly formed asexual urediniospores. Laser capture microdissection (LCM) was used to isolate three areas corresponding to uredinia and subjacent zones in the host mesophyll for expression analysis with M. larici-populina whole-genome exon oligoarrays. Optimization of tissue preparation prior to LCM allowed isolation of RNA of good integrity for genome-wide expression profiling. Our results indicate that the poplar rust uredinial stage is marked by distinct genetic programs related to biotrophy in the host palisade mesophyll and to sporulation in the uredinium. A strong induction of transcripts encoding small secreted proteins, likely containing rust effectors, is observed in the mesophyll, suggesting a late maintenance of suppression of host defense in the tissue containing haustoria and infection hyphae. On the other hand, cell cycle and cell defense rescue transcripts are strongly accumulated in the sporulation area. This combined LCM-transcriptomic approach brings new insights on the molecular mechanisms underlying urediniospore formation in rust fungi.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20831407</PMID>
<DateCompleted><Year>2010</Year>
<Month>12</Month>
<Day>02</Day>
</DateCompleted>
<DateRevised><Year>2010</Year>
<Month>09</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN>
<JournalIssue CitedMedium="Print"><Volume>23</Volume>
<Issue>10</Issue>
<PubDate><Year>2010</Year>
<Month>Oct</Month>
</PubDate>
</JournalIssue>
<Title>Molecular plant-microbe interactions : MPMI</Title>
<ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation>
</Journal>
<ArticleTitle>Laser capture microdissection of uredinia formed by Melampsora larici-populina revealed a transcriptional switch between biotrophy and sporulation.</ArticleTitle>
<Pagination><MedlinePgn>1275-86</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-05-10-0111</ELocationID>
<Abstract><AbstractText>The foliar rust caused by the basidiomycete Melampsora larici-populina is the main disease affecting poplar plantations in Europe. The biotrophic status of rust fungi is a major limitation to study gene expression of cell or tissue types during host infection. At the uredinial stage, infected poplar leaves contain distinct rust tissues such as haustoria, infection hyphae, and uredinia with sporogenous hyphae and newly formed asexual urediniospores. Laser capture microdissection (LCM) was used to isolate three areas corresponding to uredinia and subjacent zones in the host mesophyll for expression analysis with M. larici-populina whole-genome exon oligoarrays. Optimization of tissue preparation prior to LCM allowed isolation of RNA of good integrity for genome-wide expression profiling. Our results indicate that the poplar rust uredinial stage is marked by distinct genetic programs related to biotrophy in the host palisade mesophyll and to sporulation in the uredinium. A strong induction of transcripts encoding small secreted proteins, likely containing rust effectors, is observed in the mesophyll, suggesting a late maintenance of suppression of host defense in the tissue containing haustoria and infection hyphae. On the other hand, cell cycle and cell defense rescue transcripts are strongly accumulated in the sporulation area. This combined LCM-transcriptomic approach brings new insights on the molecular mechanisms underlying urediniospore formation in rust fungi.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hacquard</LastName>
<ForeName>Stéphane</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Unité Mixte de Recherche 1136 INRA/Nancy Université Interactions Arbres/Micro-organismes, Champenoux, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Delaruelle</LastName>
<ForeName>Christine</ForeName>
<Initials>C</Initials>
</Author>
<Author ValidYN="Y"><LastName>Legué</LastName>
<ForeName>Valérie</ForeName>
<Initials>V</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tisserant</LastName>
<ForeName>Emilie</ForeName>
<Initials>E</Initials>
</Author>
<Author ValidYN="Y"><LastName>Kohler</LastName>
<ForeName>Annegret</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Frey</LastName>
<ForeName>Pascal</ForeName>
<Initials>P</Initials>
</Author>
<Author ValidYN="Y"><LastName>Martin</LastName>
<ForeName>Francis</ForeName>
<Initials>F</Initials>
</Author>
<Author ValidYN="Y"><LastName>Duplessis</LastName>
<ForeName>Sébastien</ForeName>
<Initials>S</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Mol Plant Microbe Interact</MedlineTA>
<NlmUniqueID>9107902</NlmUniqueID>
<ISSNLinking>0894-0282</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015964" MajorTopicYN="N">Gene Expression Regulation, Bacterial</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D042282" MajorTopicYN="N">Microdissection</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013171" MajorTopicYN="N">Spores, Bacterial</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year>
<Month>9</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2010</Year>
<Month>9</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2010</Year>
<Month>12</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">20831407</ArticleId>
<ArticleId IdType="doi">10.1094/MPMI-05-10-0111</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000114 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000114 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= MelampsoraV2 |flux= PubMed |étape= Corpus |type= RBID |clé= pubmed:20831407 |texte= Laser capture microdissection of uredinia formed by Melampsora larici-populina revealed a transcriptional switch between biotrophy and sporulation. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:20831407" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a MelampsoraV2
![]() | This area was generated with Dilib version V0.6.38. | ![]() |