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Transferability of SSR markers from related Uredinales species to the coffee rust Hemileia vastatrix.

Identifieur interne : 000154 ( Ncbi/Merge ); précédent : 000153; suivant : 000155

Transferability of SSR markers from related Uredinales species to the coffee rust Hemileia vastatrix.

Auteurs : M. Cristancho [Colombie] ; C. Escobar

Source :

RBID : pubmed:19048497

Descripteurs français

English descriptors

Abstract

The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR markers developed in the related Uredinales species Puccinia coronata f. sp lolli and Melampsora linii to H. vastatrix was tested. A low level of transferability of SSRs was detected, and only 4 potential markers that can be used to fingerprint the coffee rust races were identified.

DOI: 10.4238/vol7-4gmr493
PubMed: 19048497

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pubmed:19048497

Le document en format XML

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<nlm:affiliation>Plant Pathology Department, National Coffee Research Centre, CENICAFE-Colombia National Center for Coffee Research, Plan Alto, Chinchiná-Caldas, Colombia. marco.cristancho@cafedecolombia.com</nlm:affiliation>
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<div type="abstract" xml:lang="en">The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR markers developed in the related Uredinales species Puccinia coronata f. sp lolli and Melampsora linii to H. vastatrix was tested. A low level of transferability of SSRs was detected, and only 4 potential markers that can be used to fingerprint the coffee rust races were identified.</div>
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<AbstractText>The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR markers developed in the related Uredinales species Puccinia coronata f. sp lolli and Melampsora linii to H. vastatrix was tested. A low level of transferability of SSRs was detected, and only 4 potential markers that can be used to fingerprint the coffee rust races were identified.</AbstractText>
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