Transferability of SSR markers from related Uredinales species to the coffee rust Hemileia vastatrix.
Identifieur interne : 000130 ( PubMed/Checkpoint ); précédent : 000129; suivant : 000131Transferability of SSR markers from related Uredinales species to the coffee rust Hemileia vastatrix.
Auteurs : M. Cristancho [Colombie] ; C. EscobarSource :
- Genetics and molecular research : GMR [ 1676-5680 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Basidiomycota, Répétitions microsatellites.
- microbiologie : Coffea, Maladies des plantes.
- Génome végétal, Spécificité d'espèce, Transfert horizontal de gène.
English descriptors
- KwdEn :
- MESH :
- genetics : Basidiomycota, Microsatellite Repeats.
- microbiology : Coffea, Plant Diseases.
- Gene Transfer, Horizontal, Genome, Plant, Species Specificity.
Abstract
The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR markers developed in the related Uredinales species Puccinia coronata f. sp lolli and Melampsora linii to H. vastatrix was tested. A low level of transferability of SSRs was detected, and only 4 potential markers that can be used to fingerprint the coffee rust races were identified.
DOI: 10.4238/vol7-4gmr493
PubMed: 19048497
Affiliations:
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pubmed:19048497Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, National Coffee Research Centre, CENICAFE-Colombia National Center for Coffee Research, Plan Alto, Chinchiná-Caldas, Colombia. marco.cristancho@cafedecolombia.com</nlm:affiliation>
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<author><name sortKey="Escobar, C" sort="Escobar, C" uniqKey="Escobar C" first="C" last="Escobar">C. Escobar</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term>
<term>Coffea (microbiology)</term>
<term>Gene Transfer, Horizontal (MeSH)</term>
<term>Genome, Plant (MeSH)</term>
<term>Microsatellite Repeats (genetics)</term>
<term>Plant Diseases (microbiology)</term>
<term>Species Specificity (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term>
<term>Coffea (microbiologie)</term>
<term>Génome végétal (MeSH)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Répétitions microsatellites (génétique)</term>
<term>Spécificité d'espèce (MeSH)</term>
<term>Transfert horizontal de gène (MeSH)</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Microsatellite Repeats</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term>
<term>Répétitions microsatellites</term>
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<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Coffea</term>
<term>Maladies des plantes</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Coffea</term>
<term>Plant Diseases</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Transfer, Horizontal</term>
<term>Genome, Plant</term>
<term>Species Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Génome végétal</term>
<term>Spécificité d'espèce</term>
<term>Transfert horizontal de gène</term>
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<front><div type="abstract" xml:lang="en">The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR markers developed in the related Uredinales species Puccinia coronata f. sp lolli and Melampsora linii to H. vastatrix was tested. A low level of transferability of SSRs was detected, and only 4 potential markers that can be used to fingerprint the coffee rust races were identified.</div>
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<Abstract><AbstractText>The aim of the present research was to test the transferability of simple sequence repeat (SSR) markers developed in two Uredinales species to Hemileia vastatrix, coffee rust. The development of efficient techniques for the identification of H. vastatrix isolates is imperative, given the continuous development of new races. The transferability of 25 SSR markers developed in the related Uredinales species Puccinia coronata f. sp lolli and Melampsora linii to H. vastatrix was tested. A low level of transferability of SSRs was detected, and only 4 potential markers that can be used to fingerprint the coffee rust races were identified.</AbstractText>
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<AffiliationInfo><Affiliation>Plant Pathology Department, National Coffee Research Centre, CENICAFE-Colombia National Center for Coffee Research, Plan Alto, Chinchiná-Caldas, Colombia. marco.cristancho@cafedecolombia.com</Affiliation>
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