The AvrM Effector from Flax Rust Has a Structured C-Terminal Domain and Interacts Directly with the M Resistance Protein
Identifieur interne : 000189 ( Ncbi/Checkpoint ); précédent : 000188; suivant : 000190The AvrM Effector from Flax Rust Has a Structured C-Terminal Domain and Interacts Directly with the M Resistance Protein
Auteurs : Ann-Maree Catanzariti [États-Unis] ; Peter N. Dodds [Australie] ; Thomas Ve [Australie] ; Bostjan Kobe [Australie] ; Jeffrey G. Ellis [Australie] ; Brian J. Staskawicz [États-Unis]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2010.
Abstract
In plant immunity, recognition of pathogen effectors by plant resistance proteins leads to the activation of plant defenses and a localized cell death response. The AvrM effector from flax rust is a small secreted protein that is recognized by the M resistance protein in flax. Here, we investigate the mechanism of M–AvrM recognition and show that these two proteins directly interact in a yeast two-hybrid assay, and that this interaction correlates with the recognition specificity observed for each of the different AvrM variants. We further characterize this interaction by demonstrating that the C-terminal domain of AvrM is required for M-dependent cell death, and show that this domain also interacts with the M protein in yeast. We investigate the role of C-terminal differences among the different AvrM proteins for their involvement in this interaction and establish that M recognition is hindered by an additional 34 amino acids present at the C terminus of several AvrM variants. Structural characterization of recombinant AvrM-A protein revealed a globular C-terminal domain that dimerizes.
Url:
DOI: 10.1094/MPMI-23-1-0049
PubMed: 19958138
PubMed Central: 3142614
Affiliations:
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Dodds</name><affiliation wicri:level="1"><nlm:aff id="A2"> Commonwealth Scientific and Industrial Research Organisation, Division of Plant Industry, GPO Box 1600, Canberra ACT 2601, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea> Commonwealth Scientific and Industrial Research Organisation, Division of Plant Industry, GPO Box 1600, Canberra ACT 2601</wicri:regionArea><wicri:noRegion>Canberra ACT 2601</wicri:noRegion></affiliation></author><author><name sortKey="Ve, Thomas" sort="Ve, Thomas" uniqKey="Ve T" first="Thomas" last="Ve">Thomas Ve</name><affiliation wicri:level="1"><nlm:aff id="A3"> School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea> School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072</wicri:regionArea><wicri:noRegion>Brisbane QLD 4072</wicri:noRegion></affiliation></author><author><name sortKey="Kobe, Bostjan" sort="Kobe, Bostjan" uniqKey="Kobe B" first="Bostjan" last="Kobe">Bostjan Kobe</name><affiliation wicri:level="1"><nlm:aff id="A3"> School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea> School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072</wicri:regionArea><wicri:noRegion>Brisbane QLD 4072</wicri:noRegion></affiliation></author><author><name sortKey="Ellis, Jeffrey G" sort="Ellis, Jeffrey G" uniqKey="Ellis J" first="Jeffrey G." last="Ellis">Jeffrey G. 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Staskawicz</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley 94720-3102, U.S.A</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea> Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley 94720-3102</wicri:regionArea><placeName><settlement type="city">Berkeley (Californie)</settlement><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">In plant immunity, recognition of pathogen effectors by plant resistance proteins leads to the activation of plant defenses and a localized cell death response. The AvrM effector from flax rust is a small secreted protein that is recognized by the M resistance protein in flax. Here, we investigate the mechanism of M–AvrM recognition and show that these two proteins directly interact in a yeast two-hybrid assay, and that this interaction correlates with the recognition specificity observed for each of the different AvrM variants. We further characterize this interaction by demonstrating that the C-terminal domain of AvrM is required for M-dependent cell death, and show that this domain also interacts with the M protein in yeast. We investigate the role of C-terminal differences among the different AvrM proteins for their involvement in this interaction and establish that M recognition is hindered by an additional 34 amino acids present at the C terminus of several AvrM variants. Structural characterization of recombinant AvrM-A protein revealed a globular C-terminal domain that dimerizes.</p></div></front></TEI><affiliations><list><country><li>Australie</li><li>États-Unis</li></country><region><li>Californie</li></region><settlement><li>Berkeley (Californie)</li></settlement></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Catanzariti, Ann Maree" sort="Catanzariti, Ann Maree" uniqKey="Catanzariti A" first="Ann-Maree" last="Catanzariti">Ann-Maree Catanzariti</name></region><name sortKey="Staskawicz, Brian J" sort="Staskawicz, Brian J" uniqKey="Staskawicz B" first="Brian J." last="Staskawicz">Brian J. Staskawicz</name></country><country name="Australie"><noRegion><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name></noRegion><name sortKey="Ellis, Jeffrey G" sort="Ellis, Jeffrey G" uniqKey="Ellis J" first="Jeffrey G." last="Ellis">Jeffrey G. Ellis</name><name sortKey="Kobe, Bostjan" sort="Kobe, Bostjan" uniqKey="Kobe B" first="Bostjan" last="Kobe">Bostjan Kobe</name><name sortKey="Ve, Thomas" sort="Ve, Thomas" uniqKey="Ve T" first="Thomas" last="Ve">Thomas Ve</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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