Serveur d'exploration Melampsora (ISTEX)

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Identification of a Differentially Expressed TIR-NBS-LRR Gene in a Major QTL Associated to Leaf Rust Resistance in Salix

Identifieur interne : 000632 ( Main/Exploration ); précédent : 000631; suivant : 000633

Identification of a Differentially Expressed TIR-NBS-LRR Gene in a Major QTL Associated to Leaf Rust Resistance in Salix

Auteurs : Tom Martin [Suède] ; Ann-Christin Rönnberg-W Stljung [Suède] ; Jan Stenlid [Suède] ; Berit Samils [Suède]

Source :

RBID : PMC:5176316

Descripteurs français

English descriptors

Abstract

An earlier identified major quantitative trait locus for resistance towards the willow leaf rust fungus Melampsora larici-epitea in a Salix viminalis x (S. viminalis × S. schwerinii) population was used to identify potential resistance genes to the rust pathogen. Screening a genomic bacterial artificial chromosome library with markers from the peak position of the QTL region revealed one gene with TIR-NBS-LRR (Toll Interleukin1 Receptor-Nucleotide Binding Site-Leucine-Rich Repeat) domain structure indicative of a resistance gene. The resistance gene analog was denoted RGA1 and further analysis revealed a number of non-synonymous single nucleotide polymorphisms in the LRR domain between the resistant and susceptible Salix genotypes. Gene expression levels under controlled conditions showed a significantly lower constitutive expression of RGA1 in the susceptible genotype. In addition, the susceptible genotype showed a significantly reduced expression level of the RGA1 gene at 24 hours post inoculation with M. larici-epitea. This indicates that the pathogen may actively suppress RGA1 gene expression allowing a compatible plant-pathogen interaction and causing infection.


Url:
DOI: 10.1371/journal.pone.0168776
PubMed: 28002449
PubMed Central: 5176316


Affiliations:


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Le document en format XML

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<term>Amino Acid Sequence (MeSH)</term>
<term>Basidiomycota (pathogenicity)</term>
<term>Chromosomes, Artificial, Bacterial (genetics)</term>
<term>Chromosomes, Artificial, Bacterial (metabolism)</term>
<term>Disease Resistance (genetics)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genotype (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (microbiology)</term>
<term>Plant Proteins (chemistry)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Polymorphism, Single Nucleotide (MeSH)</term>
<term>Protein Structure, Tertiary (MeSH)</term>
<term>Quantitative Trait Loci (MeSH)</term>
<term>Salix (genetics)</term>
<term>Salix (microbiology)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Toll-Like Receptor 1 (chemistry)</term>
<term>Toll-Like Receptor 1 (genetics)</term>
<term>Toll-Like Receptor 1 (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>Alignement de séquences (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Basidiomycota (pathogénicité)</term>
<term>Chromosomes artificiels de bactérie (génétique)</term>
<term>Chromosomes artificiels de bactérie (métabolisme)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (microbiologie)</term>
<term>Génotype (MeSH)</term>
<term>Locus de caractère quantitatif (MeSH)</term>
<term>Maladies des plantes (génétique)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Polymorphisme de nucléotide simple (MeSH)</term>
<term>Protéines végétales (composition chimique)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Récepteur de type Toll-1 (composition chimique)</term>
<term>Récepteur de type Toll-1 (génétique)</term>
<term>Récepteur de type Toll-1 (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Résistance à la maladie (génétique)</term>
<term>Salix (génétique)</term>
<term>Salix (microbiologie)</term>
<term>Structure tertiaire des protéines (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en">
<term>Plant Proteins</term>
<term>Toll-Like Receptor 1</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr">
<term>Protéines végétales</term>
<term>Récepteur de type Toll-1</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Chromosomes, Artificial, Bacterial</term>
<term>Disease Resistance</term>
<term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Plant Proteins</term>
<term>Salix</term>
<term>Toll-Like Receptor 1</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>Chromosomes artificiels de bactérie</term>
<term>Feuilles de plante</term>
<term>Maladies des plantes</term>
<term>Protéines végétales</term>
<term>Récepteur de type Toll-1</term>
<term>Résistance à la maladie</term>
<term>Salix</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en">
<term>Chromosomes, Artificial, Bacterial</term>
<term>Plant Proteins</term>
<term>Toll-Like Receptor 1</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr">
<term>Feuilles de plante</term>
<term>Maladies des plantes</term>
<term>Salix</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en">
<term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Salix</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr">
<term>Chromosomes artificiels de bactérie</term>
<term>Protéines végétales</term>
<term>Récepteur de type Toll-1</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en">
<term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr">
<term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Amino Acid Sequence</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genotype</term>
<term>Molecular Sequence Data</term>
<term>Polymorphism, Single Nucleotide</term>
<term>Protein Structure, Tertiary</term>
<term>Quantitative Trait Loci</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Alignement de séquences</term>
<term>Analyse de séquence d'ADN</term>
<term>Données de séquences moléculaires</term>
<term>Génotype</term>
<term>Locus de caractère quantitatif</term>
<term>Polymorphisme de nucléotide simple</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
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<front>
<div type="abstract" xml:lang="en">
<p>An earlier identified major quantitative trait locus for resistance towards the willow leaf rust fungus
<italic>Melampsora larici-epitea</italic>
in a
<italic>Salix viminalis</italic>
x (
<italic>S</italic>
.
<italic>viminalis</italic>
×
<italic>S</italic>
.
<italic>schwerinii</italic>
) population was used to identify potential resistance genes to the rust pathogen. Screening a genomic bacterial artificial chromosome library with markers from the peak position of the QTL region revealed one gene with TIR-NBS-LRR (Toll Interleukin1 Receptor-Nucleotide Binding Site-Leucine-Rich Repeat) domain structure indicative of a resistance gene. The resistance gene analog was denoted RGA1 and further analysis revealed a number of non-synonymous single nucleotide polymorphisms in the LRR domain between the resistant and susceptible
<italic>Salix</italic>
genotypes. Gene expression levels under controlled conditions showed a significantly lower constitutive expression of RGA1 in the susceptible genotype. In addition, the susceptible genotype showed a significantly reduced expression level of the RGA1 gene at 24 hours post inoculation with
<italic>M</italic>
.
<italic>larici-epitea</italic>
. This indicates that the pathogen may actively suppress RGA1 gene expression allowing a compatible plant-pathogen interaction and causing infection.</p>
</div>
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