The Gene Product Specified by a Double Stranded RNA in the Flax Rust ( Melampsora lini ) is Expressed during Rust Growth
Identifieur interne : 001211 ( Main/Exploration ); précédent : 001210; suivant : 001212The Gene Product Specified by a Double Stranded RNA in the Flax Rust ( Melampsora lini ) is Expressed during Rust Growth
Auteurs : Ren Zhang [Australie] ; Matthew Dickminson [Australie, Royaume-Uni] ; Anthony Pryor [Australie]Source :
- Virus Genes [ 0920-8569 ] ; 2004.
Descripteurs français
- KwdFr :
- ARN double brin (MeSH), ARN fongique (MeSH), Basidiomycota (croissance et développement), Basidiomycota (génétique), Cadres ouverts de lecture (MeSH), Expression des gènes (MeSH), Masse moléculaire (MeSH), Protéines fongiques (composition chimique), Protéines fongiques (génétique), Spores fongiques (composition chimique), Spores fongiques (croissance et développement), Spores fongiques (génétique).
- MESH :
- composition chimique : Protéines fongiques, Spores fongiques.
- croissance et développement : Basidiomycota, Spores fongiques.
- génétique : Basidiomycota, Protéines fongiques, Spores fongiques.
- ARN double brin, ARN fongique, Cadres ouverts de lecture, Expression des gènes, Masse moléculaire.
English descriptors
- KwdEn :
- Basidiomycota (genetics), Basidiomycota (growth & development), Fungal Proteins (chemistry), Fungal Proteins (genetics), Gene Expression (MeSH), Molecular Weight (MeSH), Open Reading Frames (MeSH), RNA, Double-Stranded (MeSH), RNA, Fungal (MeSH), Spores, Fungal (chemistry), Spores, Fungal (genetics), Spores, Fungal (growth & development).
- MESH :
- chemical , chemistry : Fungal Proteins.
- chemistry : Spores, Fungal.
- genetics : Basidiomycota, Fungal Proteins, Spores, Fungal.
- growth & development : Basidiomycota, Spores, Fungal.
- Gene Expression, Molecular Weight, Open Reading Frames, RNA, Double-Stranded, RNA, Fungal.
Abstract
Abstract: Strain SP6 of the flax rust (Melampsora lini) contains 11 double-stranded RNAs (dsRNAs) of unknown function. A large open-reading frame (B3ORF1) in dsRNA B3 encodes a polypeptide of 614 amino acids, and using an antiserum raised against the B3ORF1-glutathione S-transferase fusion protein prepared from a bacterial expression system, we have detected the presence of a 67 kDa polypeptide in rust urediospores. This polypeptide, identical in size to that of the predicted translation product of B3ORF1, was not detected in spores from either a fungal strain lacking the B3 dsRNA or an isogenic strain containing no dsRNA. These data indicate that B3ORF1 present in the flax rust B3 dsRNA is expressed in vivo which warrants farther investigation in search for its function during rust development.
Url:
DOI: 10.1007/s11262-004-7431-2
Affiliations:
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Le document en format XML
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A large open-reading frame (B3ORF1) in dsRNA B3 encodes a polypeptide of 614 amino acids, and using an antiserum raised against the B3ORF1-glutathione S-transferase fusion protein prepared from a bacterial expression system, we have detected the presence of a 67 kDa polypeptide in rust urediospores. This polypeptide, identical in size to that of the predicted translation product of B3ORF1, was not detected in spores from either a fungal strain lacking the B3 dsRNA or an isogenic strain containing no dsRNA. These data indicate that B3ORF1 present in the flax rust B3 dsRNA is expressed in vivo which warrants farther investigation in search for its function during rust development.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Nottinghamshire</li></region><settlement><li>Nottingham</li></settlement><orgName><li>Université de Nottingham</li></orgName></list><tree><country name="Australie"><noRegion><name sortKey="Zhang, Ren" sort="Zhang, Ren" uniqKey="Zhang R" first="Ren" last="Zhang">Ren Zhang</name></noRegion><name sortKey="Dickminson, Matthew" sort="Dickminson, Matthew" uniqKey="Dickminson M" first="Matthew" last="Dickminson">Matthew Dickminson</name><name sortKey="Pryor, Anthony" sort="Pryor, Anthony" uniqKey="Pryor A" first="Anthony" last="Pryor">Anthony Pryor</name><name sortKey="Zhang, Ren" sort="Zhang, Ren" uniqKey="Zhang R" first="Ren" last="Zhang">Ren Zhang</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Dickminson, Matthew" sort="Dickminson, Matthew" uniqKey="Dickminson M" first="Matthew" last="Dickminson">Matthew Dickminson</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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