The gene product specified by a double stranded RNA in the flax rust (Melampsora lini) is expressed during rust growth.
Identifieur interne : 000172 ( PubMed/Corpus ); précédent : 000171; suivant : 000173The gene product specified by a double stranded RNA in the flax rust (Melampsora lini) is expressed during rust growth.
Auteurs : Ren Zhang ; Matthew J. Dickminson ; Anthony J. PryorSource :
- Virus genes [ 0920-8569 ] ; 2004.
English descriptors
- KwdEn :
- Basidiomycota (genetics), Basidiomycota (growth & development), Fungal Proteins (chemistry), Fungal Proteins (genetics), Gene Expression (MeSH), Molecular Weight (MeSH), Open Reading Frames (MeSH), RNA, Double-Stranded (MeSH), RNA, Fungal (MeSH), Spores, Fungal (chemistry), Spores, Fungal (genetics), Spores, Fungal (growth & development).
- MESH :
- chemical , chemistry : Fungal Proteins.
- chemistry : Spores, Fungal.
- genetics : Basidiomycota, Fungal Proteins, Spores, Fungal.
- growth & development : Basidiomycota, Spores, Fungal.
- Gene Expression, Molecular Weight, Open Reading Frames, RNA, Double-Stranded, RNA, Fungal.
Abstract
Strain SP6 of the flax rust (Melampsora lini) contains 11 double-stranded RNAs (dsRNAs) of unknown function. A large open-reading frame (B3ORF1 in dsRNA B3 encodes a polypeptide of 614 amino acids, and using an antiserum raised against the B3ORF1-glutathione S-transferase fusion protein prepared from a bacterial expression system, we have detected the presence of a 67 kDa polypeptide in rust urediospores. This polypeptide, identical in size to that of the predicted translation product of B3ORF1 , was not detected in spores from either a fungal strain lacking the B3 dsRNA or an isogenic strain containing no dsRNA. These data indicate that B3ORF1 present in the flax rust B3 dsRNA is expressed in vivo which warrants farther investigation in search for its function during rust development.
DOI: 10.1007/s11262-004-7431-2
PubMed: 15550768
Links to Exploration step
pubmed:15550768Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The gene product specified by a double stranded RNA in the flax rust (Melampsora lini) is expressed during rust growth.</title><author><name sortKey="Zhang, Ren" sort="Zhang, Ren" uniqKey="Zhang R" first="Ren" last="Zhang">Ren Zhang</name><affiliation><nlm:affiliation>CSIRO, Division of Plant Industry, GPO Box 1600 Canberra, ACT 2601, Australia. rzhang@uow.edu.au</nlm:affiliation></affiliation></author><author><name sortKey="Dickminson, Matthew J" sort="Dickminson, Matthew J" uniqKey="Dickminson M" first="Matthew J" last="Dickminson">Matthew J. Dickminson</name></author><author><name sortKey="Pryor, Anthony J" sort="Pryor, Anthony J" uniqKey="Pryor A" first="Anthony J" last="Pryor">Anthony J. Pryor</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15550768</idno><idno type="pmid">15550768</idno><idno type="doi">10.1007/s11262-004-7431-2</idno><idno type="wicri:Area/PubMed/Corpus">000172</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000172</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The gene product specified by a double stranded RNA in the flax rust (Melampsora lini) is expressed during rust growth.</title><author><name sortKey="Zhang, Ren" sort="Zhang, Ren" uniqKey="Zhang R" first="Ren" last="Zhang">Ren Zhang</name><affiliation><nlm:affiliation>CSIRO, Division of Plant Industry, GPO Box 1600 Canberra, ACT 2601, Australia. rzhang@uow.edu.au</nlm:affiliation></affiliation></author><author><name sortKey="Dickminson, Matthew J" sort="Dickminson, Matthew J" uniqKey="Dickminson M" first="Matthew J" last="Dickminson">Matthew J. Dickminson</name></author><author><name sortKey="Pryor, Anthony J" sort="Pryor, Anthony J" uniqKey="Pryor A" first="Anthony J" last="Pryor">Anthony J. Pryor</name></author></analytic><series><title level="j">Virus genes</title><idno type="ISSN">0920-8569</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term><term>Basidiomycota (growth & development)</term><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (genetics)</term><term>Gene Expression (MeSH)</term><term>Molecular Weight (MeSH)</term><term>Open Reading Frames (MeSH)</term><term>RNA, Double-Stranded (MeSH)</term><term>RNA, Fungal (MeSH)</term><term>Spores, Fungal (chemistry)</term><term>Spores, Fungal (genetics)</term><term>Spores, Fungal (growth & development)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Fungal Proteins</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Basidiomycota</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Molecular Weight</term><term>Open Reading Frames</term><term>RNA, Double-Stranded</term><term>RNA, Fungal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Strain SP6 of the flax rust (Melampsora lini) contains 11 double-stranded RNAs (dsRNAs) of unknown function. A large open-reading frame (B3ORF1 in dsRNA B3 encodes a polypeptide of 614 amino acids, and using an antiserum raised against the B3ORF1-glutathione S-transferase fusion protein prepared from a bacterial expression system, we have detected the presence of a 67 kDa polypeptide in rust urediospores. This polypeptide, identical in size to that of the predicted translation product of B3ORF1 , was not detected in spores from either a fungal strain lacking the B3 dsRNA or an isogenic strain containing no dsRNA. These data indicate that B3ORF1 present in the flax rust B3 dsRNA is expressed in vivo which warrants farther investigation in search for its function during rust development.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15550768</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0920-8569</ISSN><JournalIssue CitedMedium="Print"><Volume>29</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Virus genes</Title><ISOAbbreviation>Virus Genes</ISOAbbreviation></Journal><ArticleTitle>The gene product specified by a double stranded RNA in the flax rust (Melampsora lini) is expressed during rust growth.</ArticleTitle><Pagination><MedlinePgn>297-301</MedlinePgn></Pagination><Abstract><AbstractText>Strain SP6 of the flax rust (Melampsora lini) contains 11 double-stranded RNAs (dsRNAs) of unknown function. A large open-reading frame (B3ORF1 in dsRNA B3 encodes a polypeptide of 614 amino acids, and using an antiserum raised against the B3ORF1-glutathione S-transferase fusion protein prepared from a bacterial expression system, we have detected the presence of a 67 kDa polypeptide in rust urediospores. This polypeptide, identical in size to that of the predicted translation product of B3ORF1 , was not detected in spores from either a fungal strain lacking the B3 dsRNA or an isogenic strain containing no dsRNA. These data indicate that B3ORF1 present in the flax rust B3 dsRNA is expressed in vivo which warrants farther investigation in search for its function during rust development.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ren</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>CSIRO, Division of Plant Industry, GPO Box 1600 Canberra, ACT 2601, Australia. rzhang@uow.edu.au</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dickminson</LastName><ForeName>Matthew J</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Pryor</LastName><ForeName>Anthony J</ForeName><Initials>AJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virus Genes</MedlineTA><NlmUniqueID>8803967</NlmUniqueID><ISSNLinking>0920-8569</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012330">RNA, Double-Stranded</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012331">RNA, Fungal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012330" MajorTopicYN="Y">RNA, Double-Stranded</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012331" MajorTopicYN="Y">RNA, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>11</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>5</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>11</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15550768</ArticleId><ArticleId IdType="pii">5277431</ArticleId><ArticleId IdType="doi">10.1007/s11262-004-7431-2</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Gene. 1988 Jul 15;67(1):31-40</Citation><ArticleIdList><ArticleId IdType="pubmed">3047011</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 1990;28:37-58</Citation><ArticleIdList><ArticleId IdType="pubmed">20540602</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7996-8000</Citation><ArticleIdList><ArticleId IdType="pubmed">8755591</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 1998 Jul;11(7):601-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9650294</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1994 Dec 1;13(23):5616-23</Citation><ArticleIdList><ArticleId IdType="pubmed">7988558</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 1993 Nov;24(5):428-32</Citation><ArticleIdList><ArticleId IdType="pubmed">8299159</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1990 Oct 5;215(3):403-10</Citation><ArticleIdList><ArticleId IdType="pubmed">2231712</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1998 May 26;95(11):6425-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9600982</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000172 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000172 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MelampsoraV2
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:15550768
|texte= The gene product specified by a double stranded RNA in the flax rust (Melampsora lini) is expressed during rust growth.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:15550768" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MelampsoraV2
| This area was generated with Dilib version V0.6.38. |  |