TtcA a new tRNA-thioltransferase with an Fe-S cluster.
Identifieur interne : 000588 ( Main/Exploration ); précédent : 000587; suivant : 000589TtcA a new tRNA-thioltransferase with an Fe-S cluster.
Auteurs : Denis Bouvier [France] ; Natty Labessan [France] ; Martin Clémancey [France] ; Jean-Marc Latour [France] ; Jean-Luc Ravanat [France] ; Marc Fontecave [France] ; Mohamed Atta [France]Source :
- Nucleic acids research [ 1362-4962 ] ; 2014.
Descripteurs français
- KwdFr :
- Clonage moléculaire (MeSH), Cystéine (composition chimique), Escherichia coli (enzymologie), Expression des gènes (MeSH), Ferrosulfoprotéines (composition chimique), Ferrosulfoprotéines (génétique), Ferrosulfoprotéines (isolement et purification), Ferrosulfoprotéines (métabolisme), Glutarédoxines (composition chimique), Glutarédoxines (génétique), Glutarédoxines (isolement et purification), Glutarédoxines (métabolisme), Protéines Escherichia coli (composition chimique), Protéines Escherichia coli (génétique), Protéines Escherichia coli (isolement et purification), Protéines Escherichia coli (métabolisme), Sulfurtransferases (composition chimique), Sulfurtransferases (génétique), Sulfurtransferases (isolement et purification), Sulfurtransferases (métabolisme).
- MESH :
- composition chimique : Cystéine, Ferrosulfoprotéines, Glutarédoxines, Protéines Escherichia coli, Sulfurtransferases.
- enzymologie : Escherichia coli.
- génétique : Ferrosulfoprotéines, Glutarédoxines, Protéines Escherichia coli, Sulfurtransferases.
- isolement et purification : Ferrosulfoprotéines, Glutarédoxines, Protéines Escherichia coli, Sulfurtransferases.
- métabolisme : Ferrosulfoprotéines, Glutarédoxines, Protéines Escherichia coli, Sulfurtransferases.
- Clonage moléculaire, Expression des gènes.
English descriptors
- KwdEn :
- Cloning, Molecular (MeSH), Cysteine (chemistry), Escherichia coli (enzymology), Escherichia coli Proteins (chemistry), Escherichia coli Proteins (genetics), Escherichia coli Proteins (isolation & purification), Escherichia coli Proteins (metabolism), Gene Expression (MeSH), Glutaredoxins (chemistry), Glutaredoxins (genetics), Glutaredoxins (isolation & purification), Glutaredoxins (metabolism), Iron-Sulfur Proteins (chemistry), Iron-Sulfur Proteins (genetics), Iron-Sulfur Proteins (isolation & purification), Iron-Sulfur Proteins (metabolism), Sulfurtransferases (chemistry), Sulfurtransferases (genetics), Sulfurtransferases (isolation & purification), Sulfurtransferases (metabolism).
- MESH :
- chemical , chemistry : Cysteine, Escherichia coli Proteins, Glutaredoxins, Iron-Sulfur Proteins, Sulfurtransferases.
- enzymology : Escherichia coli.
- chemical , genetics : Escherichia coli Proteins, Glutaredoxins, Iron-Sulfur Proteins, Sulfurtransferases.
- chemical , isolation & purification : Escherichia coli Proteins, Glutaredoxins, Iron-Sulfur Proteins, Sulfurtransferases.
- chemical , metabolism : Escherichia coli Proteins, Glutaredoxins, Iron-Sulfur Proteins, Sulfurtransferases.
- Cloning, Molecular, Gene Expression.
Abstract
TtcA catalyzes the post-transcriptional thiolation of cytosine 32 in some tRNAs. The enzyme from Escherichia coli was homologously overexpressed in E. coli. The purified enzyme is a dimer containing an iron-sulfur cluster and displays activity in in vitro assays. The type and properties of the cluster were investigated using a combination of UV-visible absorption, EPR and Mössbauer spectroscopy, as well as by site-directed mutagenesis. These studies demonstrated that the TtcA enzyme contains a redox-active and oxygen-sensitive [4Fe-4S] cluster, chelated by only three cysteine residues and absolutely essential for activity. TtcA is unique tRNA-thiolating enzyme using an iron-sulfur cluster for catalyzing a non-redox reaction.
DOI: 10.1093/nar/gku508
PubMed: 24914049
PubMed Central: PMC4081106
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">TtcA a new tRNA-thioltransferase with an Fe-S cluster.</title>
<author><name sortKey="Bouvier, Denis" sort="Bouvier, Denis" uniqKey="Bouvier D" first="Denis" last="Bouvier">Denis Bouvier</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Labessan, Natty" sort="Labessan, Natty" uniqKey="Labessan N" first="Natty" last="Labessan">Natty Labessan</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Clemancey, Martin" sort="Clemancey, Martin" uniqKey="Clemancey M" first="Martin" last="Clémancey">Martin Clémancey</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Latour, Jean Marc" sort="Latour, Jean Marc" uniqKey="Latour J" first="Jean-Marc" last="Latour">Jean-Marc Latour</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ravanat, Jean Luc" sort="Ravanat, Jean Luc" uniqKey="Ravanat J" first="Jean-Luc" last="Ravanat">Jean-Luc Ravanat</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, INAC, SCIB, F-38000 Grenoble, France CEA, iNAC, SCIB, F-38054 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, INAC, SCIB, F-38000 Grenoble, France CEA, iNAC, SCIB, F-38054 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fontecave, Marc" sort="Fontecave, Marc" uniqKey="Fontecave M" first="Marc" last="Fontecave">Marc Fontecave</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France Laboratoire de Chimie des Processus Biologiques, UMR 8229 Collège de France/CNRS/UPMC, 11 place Marcellin-Berthelot, Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France Laboratoire de Chimie des Processus Biologiques, UMR 8229 Collège de France/CNRS/UPMC, 11 place Marcellin-Berthelot, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Atta, Mohamed" sort="Atta, Mohamed" uniqKey="Atta M" first="Mohamed" last="Atta">Mohamed Atta</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France mohamed.atta@cea.fr.</nlm:affiliation>
<country wicri:rule="url">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:24914049</idno>
<idno type="pmid">24914049</idno>
<idno type="doi">10.1093/nar/gku508</idno>
<idno type="pmc">PMC4081106</idno>
<idno type="wicri:Area/Main/Corpus">000622</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000622</idno>
<idno type="wicri:Area/Main/Curation">000622</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000622</idno>
<idno type="wicri:Area/Main/Exploration">000622</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">TtcA a new tRNA-thioltransferase with an Fe-S cluster.</title>
<author><name sortKey="Bouvier, Denis" sort="Bouvier, Denis" uniqKey="Bouvier D" first="Denis" last="Bouvier">Denis Bouvier</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Labessan, Natty" sort="Labessan, Natty" uniqKey="Labessan N" first="Natty" last="Labessan">Natty Labessan</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Clemancey, Martin" sort="Clemancey, Martin" uniqKey="Clemancey M" first="Martin" last="Clémancey">Martin Clémancey</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Latour, Jean Marc" sort="Latour, Jean Marc" uniqKey="Latour J" first="Jean-Marc" last="Latour">Jean-Marc Latour</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ravanat, Jean Luc" sort="Ravanat, Jean Luc" uniqKey="Ravanat J" first="Jean-Luc" last="Ravanat">Jean-Luc Ravanat</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, INAC, SCIB, F-38000 Grenoble, France CEA, iNAC, SCIB, F-38054 Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, INAC, SCIB, F-38000 Grenoble, France CEA, iNAC, SCIB, F-38054 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fontecave, Marc" sort="Fontecave, Marc" uniqKey="Fontecave M" first="Marc" last="Fontecave">Marc Fontecave</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France Laboratoire de Chimie des Processus Biologiques, UMR 8229 Collège de France/CNRS/UPMC, 11 place Marcellin-Berthelot, Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France Laboratoire de Chimie des Processus Biologiques, UMR 8229 Collège de France/CNRS/UPMC, 11 place Marcellin-Berthelot, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Atta, Mohamed" sort="Atta, Mohamed" uniqKey="Atta M" first="Mohamed" last="Atta">Mohamed Atta</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France mohamed.atta@cea.fr.</nlm:affiliation>
<country wicri:rule="url">France</country>
<wicri:regionArea>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Nucleic acids research</title>
<idno type="eISSN">1362-4962</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular (MeSH)</term>
<term>Cysteine (chemistry)</term>
<term>Escherichia coli (enzymology)</term>
<term>Escherichia coli Proteins (chemistry)</term>
<term>Escherichia coli Proteins (genetics)</term>
<term>Escherichia coli Proteins (isolation & purification)</term>
<term>Escherichia coli Proteins (metabolism)</term>
<term>Gene Expression (MeSH)</term>
<term>Glutaredoxins (chemistry)</term>
<term>Glutaredoxins (genetics)</term>
<term>Glutaredoxins (isolation & purification)</term>
<term>Glutaredoxins (metabolism)</term>
<term>Iron-Sulfur Proteins (chemistry)</term>
<term>Iron-Sulfur Proteins (genetics)</term>
<term>Iron-Sulfur Proteins (isolation & purification)</term>
<term>Iron-Sulfur Proteins (metabolism)</term>
<term>Sulfurtransferases (chemistry)</term>
<term>Sulfurtransferases (genetics)</term>
<term>Sulfurtransferases (isolation & purification)</term>
<term>Sulfurtransferases (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Clonage moléculaire (MeSH)</term>
<term>Cystéine (composition chimique)</term>
<term>Escherichia coli (enzymologie)</term>
<term>Expression des gènes (MeSH)</term>
<term>Ferrosulfoprotéines (composition chimique)</term>
<term>Ferrosulfoprotéines (génétique)</term>
<term>Ferrosulfoprotéines (isolement et purification)</term>
<term>Ferrosulfoprotéines (métabolisme)</term>
<term>Glutarédoxines (composition chimique)</term>
<term>Glutarédoxines (génétique)</term>
<term>Glutarédoxines (isolement et purification)</term>
<term>Glutarédoxines (métabolisme)</term>
<term>Protéines Escherichia coli (composition chimique)</term>
<term>Protéines Escherichia coli (génétique)</term>
<term>Protéines Escherichia coli (isolement et purification)</term>
<term>Protéines Escherichia coli (métabolisme)</term>
<term>Sulfurtransferases (composition chimique)</term>
<term>Sulfurtransferases (génétique)</term>
<term>Sulfurtransferases (isolement et purification)</term>
<term>Sulfurtransferases (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cysteine</term>
<term>Escherichia coli Proteins</term>
<term>Glutaredoxins</term>
<term>Iron-Sulfur Proteins</term>
<term>Sulfurtransferases</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cystéine</term>
<term>Ferrosulfoprotéines</term>
<term>Glutarédoxines</term>
<term>Protéines Escherichia coli</term>
<term>Sulfurtransferases</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Escherichia coli Proteins</term>
<term>Glutaredoxins</term>
<term>Iron-Sulfur Proteins</term>
<term>Sulfurtransferases</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ferrosulfoprotéines</term>
<term>Glutarédoxines</term>
<term>Protéines Escherichia coli</term>
<term>Sulfurtransferases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Escherichia coli Proteins</term>
<term>Glutaredoxins</term>
<term>Iron-Sulfur Proteins</term>
<term>Sulfurtransferases</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Ferrosulfoprotéines</term>
<term>Glutarédoxines</term>
<term>Protéines Escherichia coli</term>
<term>Sulfurtransferases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Escherichia coli Proteins</term>
<term>Glutaredoxins</term>
<term>Iron-Sulfur Proteins</term>
<term>Sulfurtransferases</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Ferrosulfoprotéines</term>
<term>Glutarédoxines</term>
<term>Protéines Escherichia coli</term>
<term>Sulfurtransferases</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term>
<term>Gene Expression</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term>
<term>Expression des gènes</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">TtcA catalyzes the post-transcriptional thiolation of cytosine 32 in some tRNAs. The enzyme from Escherichia coli was homologously overexpressed in E. coli. The purified enzyme is a dimer containing an iron-sulfur cluster and displays activity in in vitro assays. The type and properties of the cluster were investigated using a combination of UV-visible absorption, EPR and Mössbauer spectroscopy, as well as by site-directed mutagenesis. These studies demonstrated that the TtcA enzyme contains a redox-active and oxygen-sensitive [4Fe-4S] cluster, chelated by only three cysteine residues and absolutely essential for activity. TtcA is unique tRNA-thiolating enzyme using an iron-sulfur cluster for catalyzing a non-redox reaction. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24914049</PMID>
<DateCompleted><Year>2014</Year>
<Month>09</Month>
<Day>02</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1362-4962</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>42</Volume>
<Issue>12</Issue>
<PubDate><Year>2014</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Nucleic acids research</Title>
<ISOAbbreviation>Nucleic Acids Res</ISOAbbreviation>
</Journal>
<ArticleTitle>TtcA a new tRNA-thioltransferase with an Fe-S cluster.</ArticleTitle>
<Pagination><MedlinePgn>7960-70</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1093/nar/gku508</ELocationID>
<Abstract><AbstractText>TtcA catalyzes the post-transcriptional thiolation of cytosine 32 in some tRNAs. The enzyme from Escherichia coli was homologously overexpressed in E. coli. The purified enzyme is a dimer containing an iron-sulfur cluster and displays activity in in vitro assays. The type and properties of the cluster were investigated using a combination of UV-visible absorption, EPR and Mössbauer spectroscopy, as well as by site-directed mutagenesis. These studies demonstrated that the TtcA enzyme contains a redox-active and oxygen-sensitive [4Fe-4S] cluster, chelated by only three cysteine residues and absolutely essential for activity. TtcA is unique tRNA-thiolating enzyme using an iron-sulfur cluster for catalyzing a non-redox reaction. </AbstractText>
<CopyrightInformation>© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bouvier</LastName>
<ForeName>Denis</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Labessan</LastName>
<ForeName>Natty</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Clémancey</LastName>
<ForeName>Martin</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Latour</LastName>
<ForeName>Jean-Marc</ForeName>
<Initials>JM</Initials>
<AffiliationInfo><Affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ravanat</LastName>
<ForeName>Jean-Luc</ForeName>
<Initials>JL</Initials>
<AffiliationInfo><Affiliation>University of Grenoble Alpes, INAC, SCIB, F-38000 Grenoble, France CEA, iNAC, SCIB, F-38054 Grenoble, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fontecave</LastName>
<ForeName>Marc</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France Laboratoire de Chimie des Processus Biologiques, UMR 8229 Collège de France/CNRS/UPMC, 11 place Marcellin-Berthelot, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Atta</LastName>
<ForeName>Mohamed</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>University of Grenoble Alpes, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CNRS, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France CEA, iRTSV-LCBM, UMR5249, F-38000 Grenoble, France mohamed.atta@cea.fr.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>06</Month>
<Day>09</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Nucleic Acids Res</MedlineTA>
<NlmUniqueID>0411011</NlmUniqueID>
<ISSNLinking>0305-1048</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D029968">Escherichia coli Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007506">Iron-Sulfur Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.8.1.-</RegistryNumber>
<NameOfSubstance UI="D013466">Sulfurtransferases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.8.1.-</RegistryNumber>
<NameOfSubstance UI="C000591238">TtcA protein, E coli</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>K848JZ4886</RegistryNumber>
<NameOfSubstance UI="D003545">Cysteine</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003545" MajorTopicYN="N">Cysteine</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029968" MajorTopicYN="N">Escherichia coli Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007506" MajorTopicYN="N">Iron-Sulfur Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013466" MajorTopicYN="N">Sulfurtransferases</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year>
<Month>6</Month>
<Day>11</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2014</Year>
<Month>6</Month>
<Day>11</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2014</Year>
<Month>9</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">24914049</ArticleId>
<ArticleId IdType="pii">gku508</ArticleId>
<ArticleId IdType="doi">10.1093/nar/gku508</ArticleId>
<ArticleId IdType="pmc">PMC4081106</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Bacteriol. 2004 Feb;186(3):758-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14729702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 2004 Feb;186(3):750-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14729701</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1983 Feb 15;129(1):1-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6190418</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1983 Jun;131(2):373-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6614472</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Rev. 1988 Mar;52(1):103-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3280963</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1988;158:357-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3374387</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Chromatogr. 1989 Jun 2;471:3-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2670985</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteins. 1994 Dec;20(4):347-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7731953</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Struct Biol. 1996 Jan;3(1):74-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8548458</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1998 Jun 1;26(11):2606-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9592144</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2006 Jan 6;21(1):97-108</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16387657</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Chem Biol. 2006 Apr;2(4):185-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16547481</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 2006 Apr;188(8):3052-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16585765</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2006 May 19;281(20):14296-306</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16547008</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2007 May 1;46(17):5140-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17407324</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Soc Trans. 2008 Dec;36(Pt 6):1112-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19021507</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Chem Soc. 2011 Mar 30;133(12):4342-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21366233</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Genet. 2012;46:69-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22905870</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Chem Biol. 2013 May;9(5):333-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23542644</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Sci. 1999 Nov;8(11):2424-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10595545</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biochem. 2001 Jul;130(1):63-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11432781</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2002 Apr 19;277(16):13367-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11882645</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 2002 Dec;184(24):6820-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12446632</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 2002 Dec;184(24):6830-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12446633</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Commun (Camb). 2002 Nov 21;(22):2708-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12510310</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2003 Feb 4;42(4):1109-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12549933</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2003 Sep;12(3):689-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14527414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>France</li>
</country>
<region><li>Auvergne-Rhône-Alpes</li>
<li>Rhône-Alpes</li>
<li>Île-de-France</li>
</region>
<settlement><li>Grenoble</li>
<li>Paris</li>
</settlement>
</list>
<tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Bouvier, Denis" sort="Bouvier, Denis" uniqKey="Bouvier D" first="Denis" last="Bouvier">Denis Bouvier</name>
</region>
<name sortKey="Atta, Mohamed" sort="Atta, Mohamed" uniqKey="Atta M" first="Mohamed" last="Atta">Mohamed Atta</name>
<name sortKey="Clemancey, Martin" sort="Clemancey, Martin" uniqKey="Clemancey M" first="Martin" last="Clémancey">Martin Clémancey</name>
<name sortKey="Fontecave, Marc" sort="Fontecave, Marc" uniqKey="Fontecave M" first="Marc" last="Fontecave">Marc Fontecave</name>
<name sortKey="Labessan, Natty" sort="Labessan, Natty" uniqKey="Labessan N" first="Natty" last="Labessan">Natty Labessan</name>
<name sortKey="Latour, Jean Marc" sort="Latour, Jean Marc" uniqKey="Latour J" first="Jean-Marc" last="Latour">Jean-Marc Latour</name>
<name sortKey="Ravanat, Jean Luc" sort="Ravanat, Jean Luc" uniqKey="Ravanat J" first="Jean-Luc" last="Ravanat">Jean-Luc Ravanat</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GlutaredoxinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000588 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000588 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= GlutaredoxinV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:24914049 |texte= TtcA a new tRNA-thioltransferase with an Fe-S cluster. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24914049" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a GlutaredoxinV1
This area was generated with Dilib version V0.6.37. |