Lon domain-containing protein 1 represses thioredoxin y2 and regulates ROS levels in Arabidopsis chloroplasts.
Identifieur interne : 000092 ( Main/Corpus ); précédent : 000091; suivant : 000093Lon domain-containing protein 1 represses thioredoxin y2 and regulates ROS levels in Arabidopsis chloroplasts.
Auteurs : Jin Seok Shin ; Soo Youn Kim ; Won Mi So ; Minsoo Noh ; Kyoung Shin Yoo ; Jeong Sheop ShinSource :
- FEBS letters [ 1873-3468 ] ; 2020.
Abstract
Plant thioredoxins (Trxs) act as antioxidants and function as redox regulators in the chloroplast. Although the regulation of ROS in chloroplasts is well elucidated, the precise regulation mechanism of Trx remains unknown. Here, we characterize a novel chloroplast protein, Lon domain-containing protein 1 (LCP1), which contains only a Lon domain, the precise function of which is not known. We find that LCP1 interacts with Trx-y2 and represses its activity, and that knockdown (KD) of LCP1 causes anther indehiscence due to deficient lignin deposition. In addition, LCP1 KD plants show less ROS accumulation and lower expression of ROS-responsive marker genes than the wild-type plant. Taken together, we suggest that LCP1 directly regulates Trx-y2 and controls H2 O2 levels and, thereby, regulates lignin polymerization in the anther endothecium.
DOI: 10.1002/1873-3468.13664
PubMed: 31701529
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lon domain-containing protein 1 represses thioredoxin y2 and regulates ROS levels in Arabidopsis chloroplasts.</title><author><name sortKey="Shin, Jin Seok" sort="Shin, Jin Seok" uniqKey="Shin J" first="Jin Seok" last="Shin">Jin Seok Shin</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Soo Youn" sort="Kim, Soo Youn" uniqKey="Kim S" first="Soo Youn" last="Kim">Soo Youn Kim</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Bionics Inc., Seongdong-gu, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="So, Won Mi" sort="So, Won Mi" uniqKey="So W" first="Won Mi" last="So">Won Mi So</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Noh, Minsoo" sort="Noh, Minsoo" uniqKey="Noh M" first="Minsoo" last="Noh">Minsoo Noh</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Yoo, Kyoung Shin" sort="Yoo, Kyoung Shin" uniqKey="Yoo K" first="Kyoung Shin" last="Yoo">Kyoung Shin Yoo</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Shin, Jeong Sheop" sort="Shin, Jeong Sheop" uniqKey="Shin J" first="Jeong Sheop" last="Shin">Jeong Sheop Shin</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31701529</idno><idno type="pmid">31701529</idno><idno type="doi">10.1002/1873-3468.13664</idno><idno type="wicri:Area/Main/Corpus">000092</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000092</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Lon domain-containing protein 1 represses thioredoxin y2 and regulates ROS levels in Arabidopsis chloroplasts.</title><author><name sortKey="Shin, Jin Seok" sort="Shin, Jin Seok" uniqKey="Shin J" first="Jin Seok" last="Shin">Jin Seok Shin</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Soo Youn" sort="Kim, Soo Youn" uniqKey="Kim S" first="Soo Youn" last="Kim">Soo Youn Kim</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Bionics Inc., Seongdong-gu, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="So, Won Mi" sort="So, Won Mi" uniqKey="So W" first="Won Mi" last="So">Won Mi So</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Noh, Minsoo" sort="Noh, Minsoo" uniqKey="Noh M" first="Minsoo" last="Noh">Minsoo Noh</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Yoo, Kyoung Shin" sort="Yoo, Kyoung Shin" uniqKey="Yoo K" first="Kyoung Shin" last="Yoo">Kyoung Shin Yoo</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Shin, Jeong Sheop" sort="Shin, Jeong Sheop" uniqKey="Shin J" first="Jeong Sheop" last="Shin">Jeong Sheop Shin</name><affiliation><nlm:affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</nlm:affiliation></affiliation></author></analytic><series><title level="j">FEBS letters</title><idno type="eISSN">1873-3468</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant thioredoxins (Trxs) act as antioxidants and function as redox regulators in the chloroplast. Although the regulation of ROS in chloroplasts is well elucidated, the precise regulation mechanism of Trx remains unknown. Here, we characterize a novel chloroplast protein, Lon domain-containing protein 1 (LCP1), which contains only a Lon domain, the precise function of which is not known. We find that LCP1 interacts with Trx-y2 and represses its activity, and that knockdown (KD) of LCP1 causes anther indehiscence due to deficient lignin deposition. In addition, LCP1 KD plants show less ROS accumulation and lower expression of ROS-responsive marker genes than the wild-type plant. Taken together, we suggest that LCP1 directly regulates Trx-y2 and controls H<sub>2</sub> O<sub>2</sub> levels and, thereby, regulates lignin polymerization in the anther endothecium.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">31701529</PMID><DateRevised><Year>2020</Year><Month>03</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3468</ISSN><JournalIssue CitedMedium="Internet"><Volume>594</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>Mar</Month></PubDate></JournalIssue><Title>FEBS letters</Title><ISOAbbreviation>FEBS Lett</ISOAbbreviation></Journal><ArticleTitle>Lon domain-containing protein 1 represses thioredoxin y2 and regulates ROS levels in Arabidopsis chloroplasts.</ArticleTitle><Pagination><MedlinePgn>986-994</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/1873-3468.13664</ELocationID><Abstract><AbstractText>Plant thioredoxins (Trxs) act as antioxidants and function as redox regulators in the chloroplast. Although the regulation of ROS in chloroplasts is well elucidated, the precise regulation mechanism of Trx remains unknown. Here, we characterize a novel chloroplast protein, Lon domain-containing protein 1 (LCP1), which contains only a Lon domain, the precise function of which is not known. We find that LCP1 interacts with Trx-y2 and represses its activity, and that knockdown (KD) of LCP1 causes anther indehiscence due to deficient lignin deposition. In addition, LCP1 KD plants show less ROS accumulation and lower expression of ROS-responsive marker genes than the wild-type plant. Taken together, we suggest that LCP1 directly regulates Trx-y2 and controls H<sub>2</sub> O<sub>2</sub> levels and, thereby, regulates lignin polymerization in the anther endothecium.</AbstractText><CopyrightInformation>© 2019 Federation of European Biochemical Societies.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shin</LastName><ForeName>Jin Seok</ForeName><Initials>JS</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-3617-7954</Identifier><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Soo Youn</ForeName><Initials>SY</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-4498-4799</Identifier><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Bionics Inc., Seongdong-gu, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>So</LastName><ForeName>Won Mi</ForeName><Initials>WM</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-4572-3248</Identifier><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noh</LastName><ForeName>Minsoo</ForeName><Initials>M</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-1239-3783</Identifier><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yoo</LastName><ForeName>Kyoung Shin</ForeName><Initials>KS</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-5519-5568</Identifier><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shin</LastName><ForeName>Jeong Sheop</ForeName><Initials>JS</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-5944-4947</Identifier><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul, Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2014R1A2A2A01005974</GrantID><Agency>National Research Foundation of Korea (NRF) funded by the Ministry of Science</Agency><Country></Country></Grant><Grant><GrantID>2019R1F1A1060014</GrantID><Agency>National Research Foundation of Korea (NRF) funded by the Ministry of Science</Agency><Country></Country></Grant><Grant><GrantID>PJ01367001</GrantID><Agency>Next-Generation BioGreen 21 Program funded by the Rural Development Administration</Agency><Country></Country></Grant><Grant><Agency>Korea University</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEBS Lett</MedlineTA><NlmUniqueID>0155157</NlmUniqueID><ISSNLinking>0014-5793</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arabidopsis</Keyword><Keyword MajorTopicYN="N">ROS</Keyword><Keyword MajorTopicYN="N">chloroplast</Keyword><Keyword MajorTopicYN="N">dehiscence</Keyword><Keyword MajorTopicYN="N">lignification</Keyword><Keyword MajorTopicYN="N">thioredoxin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>10</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31701529</ArticleId><ArticleId IdType="doi">10.1002/1873-3468.13664</ArticleId></ArticleIdList><ReferenceList><Title>References</Title><Reference><Citation>Bonner LJ and Dickinson H (1989) Anther dehiscence in Lycopersicon esculentum Mill. 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