Assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex DNA bead-based suspension array
Identifieur interne : 000202 ( Ncbi/Curation ); précédent : 000201; suivant : 000203Assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex DNA bead-based suspension array
Auteurs : Amaia Ros-García [Espagne] ; Jesús F. Barandika [Espagne] ; Ana L. García-Pérez [Espagne] ; Ram N A. Juste [Espagne] ; Ana Hurtado [Espagne]Source :
- Parasites & Vectors [ 1756-3305 ] ; 2013.
Abstract
Piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. Detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. The purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the Basque Country (northern Spain).
A DNA bead-based suspension array using the Luminex® xMAP technology that included a generic
Piroplasms were detected in 48% (214/446) of blood samples, nearly half of them (49.1%, 105/214) as mixed infections. Five different piroplasms were identified:
The multiplex Luminex® xMAP procedure is a rapid and high throughput technique that provided highly specific and sensitive identification of single and mixed piroplasm infections in blood of sheep carriers. This study confirmed a situation of endemic stability for piroplasm infection in the region, where infection is present in the absence of clinical signs, and mountain grazing allows for sufficient inoculation rates to maintain such situation.
Url:
DOI: 10.1186/1756-3305-6-277
PubMed: 24499621
PubMed Central: 3849078
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000124
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000124
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000158
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000202
Links to Exploration step
PMC:3849078Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex DNA bead-based suspension array</title>
<author><name sortKey="Ros Garcia, Amaia" sort="Ros Garcia, Amaia" uniqKey="Ros Garcia A" first="Amaia" last="Ros-García">Amaia Ros-García</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Barandika, Jesus F" sort="Barandika, Jesus F" uniqKey="Barandika J" first="Jesús F" last="Barandika">Jesús F. Barandika</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Garcia Perez, Ana L" sort="Garcia Perez, Ana L" uniqKey="Garcia Perez A" first="Ana L" last="García-Pérez">Ana L. García-Pérez</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Juste, Ram N A" sort="Juste, Ram N A" uniqKey="Juste R" first="Ram N A" last="Juste">Ram N A. Juste</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hurtado, Ana" sort="Hurtado, Ana" uniqKey="Hurtado A" first="Ana" last="Hurtado">Ana Hurtado</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24499621</idno>
<idno type="pmc">3849078</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849078</idno>
<idno type="RBID">PMC:3849078</idno>
<idno type="doi">10.1186/1756-3305-6-277</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">000124</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000124</idno>
<idno type="wicri:Area/Pmc/Curation">000124</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000124</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000158</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000158</idno>
<idno type="wicri:Area/Ncbi/Merge">000202</idno>
<idno type="wicri:Area/Ncbi/Curation">000202</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex DNA bead-based suspension array</title>
<author><name sortKey="Ros Garcia, Amaia" sort="Ros Garcia, Amaia" uniqKey="Ros Garcia A" first="Amaia" last="Ros-García">Amaia Ros-García</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Barandika, Jesus F" sort="Barandika, Jesus F" uniqKey="Barandika J" first="Jesús F" last="Barandika">Jesús F. Barandika</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Garcia Perez, Ana L" sort="Garcia Perez, Ana L" uniqKey="Garcia Perez A" first="Ana L" last="García-Pérez">Ana L. García-Pérez</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Juste, Ram N A" sort="Juste, Ram N A" uniqKey="Juste R" first="Ram N A" last="Juste">Ram N A. Juste</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hurtado, Ana" sort="Hurtado, Ana" uniqKey="Hurtado A" first="Ana" last="Hurtado">Ana Hurtado</name>
<affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Pays basque</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Parasites & Vectors</title>
<idno type="eISSN">1756-3305</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. Detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. The purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the Basque Country (northern Spain).</p>
</sec>
<sec><title>Methods</title>
<p>A DNA bead-based suspension array using the Luminex® xMAP technology that included a generic <italic>Theileria</italic>
-<italic>Babesia</italic>
control probe, 6 species-specific probes, and an internal control probe was developed to detect and identify piroplasms that infect sheep. To monitor piroplasm infection in clinically healthy sheep from 4 flocks that share communal mountain pastures, blood samples were collected during 2 grazing seasons.</p>
</sec>
<sec><title>Results</title>
<p>Piroplasms were detected in 48% (214/446) of blood samples, nearly half of them (49.1%, 105/214) as mixed infections. Five different piroplasms were identified: <italic>Theileria</italic>
sp. OT3 in 34.8% of the samples, <italic>Theileria ovis</italic>
in 20.9%, and at lower prevalences <italic>Babesia motasi</italic>
(12.3%), <italic>Theileria luwenshuni</italic>
/OT1 (10.5%) and <italic>Babesia ovis</italic>
(6.3%). Despite differences among flocks associated to differences in management, an increasing trend in the incidence of piroplasm infection with increasing age of animals after increased tick exposure was observed. This increment could be attributed to continued re-infection associated with re-exposure to ticks at grazing. Ticks were collected from animals (4 species) and vegetation (8 species), and associations between tick abundance seasonality and risk of infection with the different piroplasms were established.</p>
</sec>
<sec><title>Conclusion</title>
<p>The multiplex Luminex® xMAP procedure is a rapid and high throughput technique that provided highly specific and sensitive identification of single and mixed piroplasm infections in blood of sheep carriers. This study confirmed a situation of endemic stability for piroplasm infection in the region, where infection is present in the absence of clinical signs, and mountain grazing allows for sufficient inoculation rates to maintain such situation.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gabi A, D" uniqKey="Gabi A D">D Gabiña</name>
</author>
<author><name sortKey="Arrese, F" uniqKey="Arrese F">F Arrese</name>
</author>
<author><name sortKey="Arranz, J" uniqKey="Arranz J">J Arranz</name>
</author>
<author><name sortKey="Beltran De Heredia, I" uniqKey="Beltran De Heredia I">I Beltran de Heredia</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nagore, D" uniqKey="Nagore D">D Nagore</name>
</author>
<author><name sortKey="Garcia Sanmartin, J" uniqKey="Garcia Sanmartin J">J García-Sanmartín</name>
</author>
<author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name>
</author>
<author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name>
</author>
<author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Preston, Pm" uniqKey="Preston P">PM Preston</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Uilenberg, G" uniqKey="Uilenberg G">G Uilenberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name>
</author>
<author><name sortKey="Luo, J" uniqKey="Luo J">J Luo</name>
</author>
<author><name sortKey="Schnittger, L" uniqKey="Schnittger L">L Schnittger</name>
</author>
<author><name sortKey="Lu, B" uniqKey="Lu B">B Lu</name>
</author>
<author><name sortKey="Beyer, D" uniqKey="Beyer D">D Beyer</name>
</author>
<author><name sortKey="Ma, M" uniqKey="Ma M">M Ma</name>
</author>
<author><name sortKey="Guan, G" uniqKey="Guan G">G Guan</name>
</author>
<author><name sortKey="Bai, Q" uniqKey="Bai Q">Q Bai</name>
</author>
<author><name sortKey="Lu, C" uniqKey="Lu C">C Lu</name>
</author>
<author><name sortKey="Ahmed, J" uniqKey="Ahmed J">J Ahmed</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name>
</author>
<author><name sortKey="Luo, J" uniqKey="Luo J">J Luo</name>
</author>
<author><name sortKey="Guan, G" uniqKey="Guan G">G Guan</name>
</author>
<author><name sortKey="Lu, B" uniqKey="Lu B">B Lu</name>
</author>
<author><name sortKey="Ma, M" uniqKey="Ma M">M Ma</name>
</author>
<author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name>
</author>
<author><name sortKey="Lu, W" uniqKey="Lu W">W Lu</name>
</author>
<author><name sortKey="Lu, C" uniqKey="Lu C">C Lu</name>
</author>
<author><name sortKey="Ahmed, J" uniqKey="Ahmed J">J Ahmed</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Uilenberg, G" uniqKey="Uilenberg G">G Uilenberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sonenshine, De" uniqKey="Sonenshine D">DE Sonenshine</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chauvin, A" uniqKey="Chauvin A">A Chauvin</name>
</author>
<author><name sortKey="Moreau, E" uniqKey="Moreau E">E Moreau</name>
</author>
<author><name sortKey="Bonnet, S" uniqKey="Bonnet S">S Bonnet</name>
</author>
<author><name sortKey="Plantard, O" uniqKey="Plantard O">O Plantard</name>
</author>
<author><name sortKey="Malandrin, L" uniqKey="Malandrin L">L Malandrin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yeruham, I" uniqKey="Yeruham I">I Yeruham</name>
</author>
<author><name sortKey="Hadani, A" uniqKey="Hadani A">A Hadani</name>
</author>
<author><name sortKey="Galker, F" uniqKey="Galker F">F Galker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Ah" uniqKey="Liu A">AH Liu</name>
</author>
<author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name>
</author>
<author><name sortKey="Guan, Gq" uniqKey="Guan G">GQ Guan</name>
</author>
<author><name sortKey="Schnittger, L" uniqKey="Schnittger L">L Schnittger</name>
</author>
<author><name sortKey="Liu, Zj" uniqKey="Liu Z">ZJ Liu</name>
</author>
<author><name sortKey="Ma, Ml" uniqKey="Ma M">ML Ma</name>
</author>
<author><name sortKey="Dang, Zs" uniqKey="Dang Z">ZS Dang</name>
</author>
<author><name sortKey="Liu, Jl" uniqKey="Liu J">JL Liu</name>
</author>
<author><name sortKey="Ren, Qy" uniqKey="Ren Q">QY Ren</name>
</author>
<author><name sortKey="Bai, Q" uniqKey="Bai Q">Q Bai</name>
</author>
<author><name sortKey="Ahmed, Js" uniqKey="Ahmed J">JS Ahmed</name>
</author>
<author><name sortKey="Luo, Jx" uniqKey="Luo J">JX Luo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schnittger, L" uniqKey="Schnittger L">L Schnittger</name>
</author>
<author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name>
</author>
<author><name sortKey="Qi, B" uniqKey="Qi B">B Qi</name>
</author>
<author><name sortKey="Gubbels, Jm" uniqKey="Gubbels J">JM Gubbels</name>
</author>
<author><name sortKey="Beyer, D" uniqKey="Beyer D">D Beyer</name>
</author>
<author><name sortKey="Niemann, S" uniqKey="Niemann S">S Niemann</name>
</author>
<author><name sortKey="Jongejan, E" uniqKey="Jongejan E">E Jongejan</name>
</author>
<author><name sortKey="Ahmed, Js" uniqKey="Ahmed J">JS Ahmed</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ros Garcia, A" uniqKey="Ros Garcia A">A Ros-García</name>
</author>
<author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name>
</author>
<author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gil Collado, J" uniqKey="Gil Collado J">J Gil-Collado</name>
</author>
<author><name sortKey="Guillen Llera, Jl" uniqKey="Guillen Llera J">JL Guillén-Llera</name>
</author>
<author><name sortKey="Zapatero Ramos, Lm" uniqKey="Zapatero Ramos L">LM Zapatero-Ramos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Manilla, G" uniqKey="Manilla G">G Manilla</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Georges, K" uniqKey="Georges K">K Georges</name>
</author>
<author><name sortKey="Loria, Gr" uniqKey="Loria G">GR Loria</name>
</author>
<author><name sortKey="Riili, S" uniqKey="Riili S">S Riili</name>
</author>
<author><name sortKey="Greco, A" uniqKey="Greco A">A Greco</name>
</author>
<author><name sortKey="Caracappa, S" uniqKey="Caracappa S">S Caracappa</name>
</author>
<author><name sortKey="Jongejan, F" uniqKey="Jongejan F">F Jongejan</name>
</author>
<author><name sortKey="Sparagano, O" uniqKey="Sparagano O">O Sparagano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name>
</author>
<author><name sortKey="Garrido, Jm" uniqKey="Garrido J">JM Garrido</name>
</author>
<author><name sortKey="Geijo, M" uniqKey="Geijo M">M Geijo</name>
</author>
<author><name sortKey="Elguezabal, N" uniqKey="Elguezabal N">N Elguezabal</name>
</author>
<author><name sortKey="Aduriz, G" uniqKey="Aduriz G">G Aduriz</name>
</author>
<author><name sortKey="Atxaerandio, R" uniqKey="Atxaerandio R">R Atxaerandio</name>
</author>
<author><name sortKey="Sevilla, I" uniqKey="Sevilla I">I Sevilla</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gubbels, Jm" uniqKey="Gubbels J">JM Gubbels</name>
</author>
<author><name sortKey="De Vos, Ap" uniqKey="De Vos A">AP de Vos</name>
</author>
<author><name sortKey="Van Der Weide, M" uniqKey="Van Der Weide M">M van der Weide</name>
</author>
<author><name sortKey="Viseras, J" uniqKey="Viseras J">J Viseras</name>
</author>
<author><name sortKey="Schouls, Lm" uniqKey="Schouls L">LM Schouls</name>
</author>
<author><name sortKey="De Vries, E" uniqKey="De Vries E">E de Vries</name>
</author>
<author><name sortKey="Jongejan, F" uniqKey="Jongejan F">F Jongejan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hoorfar, J" uniqKey="Hoorfar J">J Hoorfar</name>
</author>
<author><name sortKey="Malorny, B" uniqKey="Malorny B">B Malorny</name>
</author>
<author><name sortKey="Abdulmawjood, A" uniqKey="Abdulmawjood A">A Abdulmawjood</name>
</author>
<author><name sortKey="Cook, N" uniqKey="Cook N">N Cook</name>
</author>
<author><name sortKey="Wagner, M" uniqKey="Wagner M">M Wagner</name>
</author>
<author><name sortKey="Fach, P" uniqKey="Fach P">P Fach</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barandika, Jf" uniqKey="Barandika J">JF Barandika</name>
</author>
<author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name>
</author>
<author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name>
</author>
<author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barandika, Jf" uniqKey="Barandika J">JF Barandika</name>
</author>
<author><name sortKey="Olmeda, Sa" uniqKey="Olmeda S">SA Olmeda</name>
</author>
<author><name sortKey="Casado Nistal, Ma" uniqKey="Casado Nistal M">MA Casado-Nistal</name>
</author>
<author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name>
</author>
<author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name>
</author>
<author><name sortKey="Valcarcel, F" uniqKey="Valcarcel F">F Valcarcel</name>
</author>
<author><name sortKey="Anda, P" uniqKey="Anda P">P Anda</name>
</author>
<author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garcia Sanmartin, J" uniqKey="Garcia Sanmartin J">J García-Sanmartín</name>
</author>
<author><name sortKey="Barandika, Jf" uniqKey="Barandika J">JF Barandika</name>
</author>
<author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name>
</author>
<author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name>
</author>
<author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barandika, Jf" uniqKey="Barandika J">JF Barandika</name>
</author>
<author><name sortKey="Berriatua, E" uniqKey="Berriatua E">E Berriatua</name>
</author>
<author><name sortKey="Barral, M" uniqKey="Barral M">M Barral</name>
</author>
<author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name>
</author>
<author><name sortKey="Anda, P" uniqKey="Anda P">P Anda</name>
</author>
<author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yeruham, I" uniqKey="Yeruham I">I Yeruham</name>
</author>
<author><name sortKey="Hadani, A" uniqKey="Hadani A">A Hadani</name>
</author>
<author><name sortKey="Galker, F" uniqKey="Galker F">F Galker</name>
</author>
<author><name sortKey="Rosen, S" uniqKey="Rosen S">S Rosen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Norval, Rai" uniqKey="Norval R">RAI Norval</name>
</author>
<author><name sortKey="Perry, Bd" uniqKey="Perry B">BD Perry</name>
</author>
<author><name sortKey="Young, As" uniqKey="Young A">AS Young</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dalgliesh, Rj" uniqKey="Dalgliesh R">RJ Dalgliesh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wikel, Sk" uniqKey="Wikel S">SK Wikel</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/CheneBelgiqueV2/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000202 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000202 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= CheneBelgiqueV2 |flux= Ncbi |étape= Curation |type= RBID |clé= PMC:3849078 |texte= Assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex DNA bead-based suspension array }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:24499621" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a CheneBelgiqueV2
This area was generated with Dilib version V0.6.27. |