Assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex DNA bead-based suspension array
Identifieur interne : 000202 ( Ncbi/Merge ); précédent : 000201; suivant : 000203Assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex DNA bead-based suspension array
Auteurs : Amaia Ros-García [Espagne] ; Jesús F. Barandika [Espagne] ; Ana L. García-Pérez [Espagne] ; Ram N A. Juste [Espagne] ; Ana Hurtado [Espagne]Source :
- Parasites & Vectors [ 1756-3305 ] ; 2013.
Abstract
Piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. Detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. The purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the Basque Country (northern Spain).
A DNA bead-based suspension array using the Luminex® xMAP technology that included a generic
Piroplasms were detected in 48% (214/446) of blood samples, nearly half of them (49.1%, 105/214) as mixed infections. Five different piroplasms were identified:
The multiplex Luminex® xMAP procedure is a rapid and high throughput technique that provided highly specific and sensitive identification of single and mixed piroplasm infections in blood of sheep carriers. This study confirmed a situation of endemic stability for piroplasm infection in the region, where infection is present in the absence of clinical signs, and mountain grazing allows for sufficient inoculation rates to maintain such situation.
Url:
DOI: 10.1186/1756-3305-6-277
PubMed: 24499621
PubMed Central: 3849078
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Barandika</name><affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea><placeName><region nuts="2" type="communauté">Pays basque</region></placeName></affiliation></author><author><name sortKey="Garcia Perez, Ana L" sort="Garcia Perez, Ana L" uniqKey="Garcia Perez A" first="Ana L" last="García-Pérez">Ana L. 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Barandika</name><affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea><placeName><region nuts="2" type="communauté">Pays basque</region></placeName></affiliation></author><author><name sortKey="Garcia Perez, Ana L" sort="Garcia Perez, Ana L" uniqKey="Garcia Perez A" first="Ana L" last="García-Pérez">Ana L. García-Pérez</name><affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea><placeName><region nuts="2" type="communauté">Pays basque</region></placeName></affiliation></author><author><name sortKey="Juste, Ram N A" sort="Juste, Ram N A" uniqKey="Juste R" first="Ram N A" last="Juste">Ram N A. Juste</name><affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea><placeName><region nuts="2" type="communauté">Pays basque</region></placeName></affiliation></author><author><name sortKey="Hurtado, Ana" sort="Hurtado, Ana" uniqKey="Hurtado A" first="Ana" last="Hurtado">Ana Hurtado</name><affiliation wicri:level="2"><nlm:aff id="I1">Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160</wicri:regionArea><placeName><region nuts="2" type="communauté">Pays basque</region></placeName></affiliation></author></analytic><series><title level="j">Parasites & Vectors</title><idno type="eISSN">1756-3305</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. Detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. The purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the Basque Country (northern Spain).</p></sec><sec><title>Methods</title><p>A DNA bead-based suspension array using the Luminex® xMAP technology that included a generic <italic>Theileria</italic>-<italic>Babesia</italic> control probe, 6 species-specific probes, and an internal control probe was developed to detect and identify piroplasms that infect sheep. To monitor piroplasm infection in clinically healthy sheep from 4 flocks that share communal mountain pastures, blood samples were collected during 2 grazing seasons.</p></sec><sec><title>Results</title><p>Piroplasms were detected in 48% (214/446) of blood samples, nearly half of them (49.1%, 105/214) as mixed infections. Five different piroplasms were identified: <italic>Theileria</italic> sp. OT3 in 34.8% of the samples, <italic>Theileria ovis</italic> in 20.9%, and at lower prevalences <italic>Babesia motasi</italic> (12.3%), <italic>Theileria luwenshuni</italic>/OT1 (10.5%) and <italic>Babesia ovis</italic> (6.3%). Despite differences among flocks associated to differences in management, an increasing trend in the incidence of piroplasm infection with increasing age of animals after increased tick exposure was observed. This increment could be attributed to continued re-infection associated with re-exposure to ticks at grazing. Ticks were collected from animals (4 species) and vegetation (8 species), and associations between tick abundance seasonality and risk of infection with the different piroplasms were established.</p></sec><sec><title>Conclusion</title><p>The multiplex Luminex® xMAP procedure is a rapid and high throughput technique that provided highly specific and sensitive identification of single and mixed piroplasm infections in blood of sheep carriers. This study confirmed a situation of endemic stability for piroplasm infection in the region, where infection is present in the absence of clinical signs, and mountain grazing allows for sufficient inoculation rates to maintain such situation.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gabi A, D" uniqKey="Gabi A D">D Gabiña</name></author><author><name sortKey="Arrese, F" uniqKey="Arrese F">F Arrese</name></author><author><name sortKey="Arranz, J" uniqKey="Arranz J">J Arranz</name></author><author><name sortKey="Beltran De Heredia, I" uniqKey="Beltran De Heredia I">I Beltran de Heredia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagore, D" uniqKey="Nagore D">D Nagore</name></author><author><name sortKey="Garcia Sanmartin, J" uniqKey="Garcia Sanmartin J">J García-Sanmartín</name></author><author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name></author><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name></author><author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Preston, Pm" uniqKey="Preston P">PM Preston</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uilenberg, G" uniqKey="Uilenberg G">G Uilenberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name></author><author><name sortKey="Luo, J" uniqKey="Luo J">J Luo</name></author><author><name sortKey="Schnittger, L" uniqKey="Schnittger L">L Schnittger</name></author><author><name sortKey="Lu, B" uniqKey="Lu B">B Lu</name></author><author><name sortKey="Beyer, D" uniqKey="Beyer D">D Beyer</name></author><author><name sortKey="Ma, M" uniqKey="Ma M">M Ma</name></author><author><name sortKey="Guan, G" uniqKey="Guan G">G Guan</name></author><author><name sortKey="Bai, Q" uniqKey="Bai Q">Q Bai</name></author><author><name sortKey="Lu, C" uniqKey="Lu C">C Lu</name></author><author><name sortKey="Ahmed, J" uniqKey="Ahmed J">J Ahmed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name></author><author><name sortKey="Luo, J" uniqKey="Luo J">J Luo</name></author><author><name sortKey="Guan, G" uniqKey="Guan G">G Guan</name></author><author><name sortKey="Lu, B" uniqKey="Lu B">B Lu</name></author><author><name sortKey="Ma, M" uniqKey="Ma M">M Ma</name></author><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name></author><author><name sortKey="Lu, W" uniqKey="Lu W">W Lu</name></author><author><name sortKey="Lu, C" uniqKey="Lu C">C Lu</name></author><author><name sortKey="Ahmed, J" uniqKey="Ahmed J">J Ahmed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uilenberg, G" uniqKey="Uilenberg G">G Uilenberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sonenshine, De" uniqKey="Sonenshine D">DE Sonenshine</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chauvin, A" uniqKey="Chauvin A">A Chauvin</name></author><author><name sortKey="Moreau, E" uniqKey="Moreau E">E Moreau</name></author><author><name sortKey="Bonnet, S" uniqKey="Bonnet S">S Bonnet</name></author><author><name sortKey="Plantard, O" uniqKey="Plantard O">O Plantard</name></author><author><name sortKey="Malandrin, L" uniqKey="Malandrin L">L Malandrin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yeruham, I" uniqKey="Yeruham I">I Yeruham</name></author><author><name sortKey="Hadani, A" uniqKey="Hadani A">A Hadani</name></author><author><name sortKey="Galker, F" uniqKey="Galker F">F Galker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Ah" uniqKey="Liu A">AH Liu</name></author><author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name></author><author><name sortKey="Guan, Gq" uniqKey="Guan G">GQ Guan</name></author><author><name sortKey="Schnittger, L" uniqKey="Schnittger L">L Schnittger</name></author><author><name sortKey="Liu, Zj" uniqKey="Liu Z">ZJ Liu</name></author><author><name sortKey="Ma, Ml" uniqKey="Ma M">ML Ma</name></author><author><name sortKey="Dang, Zs" uniqKey="Dang Z">ZS Dang</name></author><author><name sortKey="Liu, Jl" uniqKey="Liu J">JL Liu</name></author><author><name sortKey="Ren, Qy" uniqKey="Ren Q">QY Ren</name></author><author><name sortKey="Bai, Q" uniqKey="Bai Q">Q Bai</name></author><author><name sortKey="Ahmed, Js" uniqKey="Ahmed J">JS Ahmed</name></author><author><name sortKey="Luo, Jx" uniqKey="Luo J">JX Luo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schnittger, L" uniqKey="Schnittger L">L Schnittger</name></author><author><name sortKey="Yin, H" uniqKey="Yin H">H Yin</name></author><author><name sortKey="Qi, B" uniqKey="Qi B">B Qi</name></author><author><name sortKey="Gubbels, Jm" uniqKey="Gubbels J">JM Gubbels</name></author><author><name sortKey="Beyer, D" uniqKey="Beyer D">D Beyer</name></author><author><name sortKey="Niemann, S" uniqKey="Niemann S">S Niemann</name></author><author><name sortKey="Jongejan, E" uniqKey="Jongejan E">E Jongejan</name></author><author><name sortKey="Ahmed, Js" uniqKey="Ahmed J">JS Ahmed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ros Garcia, A" uniqKey="Ros Garcia A">A Ros-García</name></author><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name></author><author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gil Collado, J" uniqKey="Gil Collado J">J Gil-Collado</name></author><author><name sortKey="Guillen Llera, Jl" uniqKey="Guillen Llera J">JL Guillén-Llera</name></author><author><name sortKey="Zapatero Ramos, Lm" uniqKey="Zapatero Ramos L">LM Zapatero-Ramos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Manilla, G" uniqKey="Manilla G">G Manilla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Georges, K" uniqKey="Georges K">K Georges</name></author><author><name sortKey="Loria, Gr" uniqKey="Loria G">GR Loria</name></author><author><name sortKey="Riili, S" uniqKey="Riili S">S Riili</name></author><author><name sortKey="Greco, A" uniqKey="Greco A">A Greco</name></author><author><name sortKey="Caracappa, S" uniqKey="Caracappa S">S Caracappa</name></author><author><name sortKey="Jongejan, F" uniqKey="Jongejan F">F Jongejan</name></author><author><name sortKey="Sparagano, O" uniqKey="Sparagano O">O Sparagano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name></author><author><name sortKey="Garrido, Jm" uniqKey="Garrido J">JM Garrido</name></author><author><name sortKey="Geijo, M" uniqKey="Geijo M">M Geijo</name></author><author><name sortKey="Elguezabal, N" uniqKey="Elguezabal N">N Elguezabal</name></author><author><name sortKey="Aduriz, G" uniqKey="Aduriz G">G Aduriz</name></author><author><name sortKey="Atxaerandio, R" uniqKey="Atxaerandio R">R Atxaerandio</name></author><author><name sortKey="Sevilla, I" uniqKey="Sevilla I">I Sevilla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gubbels, Jm" uniqKey="Gubbels J">JM Gubbels</name></author><author><name sortKey="De Vos, Ap" uniqKey="De Vos A">AP de Vos</name></author><author><name sortKey="Van Der Weide, M" uniqKey="Van Der Weide M">M van der Weide</name></author><author><name sortKey="Viseras, J" uniqKey="Viseras J">J Viseras</name></author><author><name sortKey="Schouls, Lm" uniqKey="Schouls L">LM Schouls</name></author><author><name sortKey="De Vries, E" uniqKey="De Vries E">E de Vries</name></author><author><name sortKey="Jongejan, F" uniqKey="Jongejan F">F Jongejan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoorfar, J" uniqKey="Hoorfar J">J Hoorfar</name></author><author><name sortKey="Malorny, B" uniqKey="Malorny B">B Malorny</name></author><author><name sortKey="Abdulmawjood, A" uniqKey="Abdulmawjood A">A Abdulmawjood</name></author><author><name sortKey="Cook, N" uniqKey="Cook N">N Cook</name></author><author><name sortKey="Wagner, M" uniqKey="Wagner M">M Wagner</name></author><author><name sortKey="Fach, P" uniqKey="Fach P">P Fach</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barandika, Jf" uniqKey="Barandika J">JF Barandika</name></author><author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name></author><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name></author><author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barandika, Jf" uniqKey="Barandika J">JF Barandika</name></author><author><name sortKey="Olmeda, Sa" uniqKey="Olmeda S">SA Olmeda</name></author><author><name sortKey="Casado Nistal, Ma" uniqKey="Casado Nistal M">MA Casado-Nistal</name></author><author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name></author><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name></author><author><name sortKey="Valcarcel, F" uniqKey="Valcarcel F">F Valcarcel</name></author><author><name sortKey="Anda, P" uniqKey="Anda P">P Anda</name></author><author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Sanmartin, J" uniqKey="Garcia Sanmartin J">J García-Sanmartín</name></author><author><name sortKey="Barandika, Jf" uniqKey="Barandika J">JF Barandika</name></author><author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name></author><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name></author><author><name sortKey="Hurtado, A" uniqKey="Hurtado A">A Hurtado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barandika, Jf" uniqKey="Barandika J">JF Barandika</name></author><author><name sortKey="Berriatua, E" uniqKey="Berriatua E">E Berriatua</name></author><author><name sortKey="Barral, M" uniqKey="Barral M">M Barral</name></author><author><name sortKey="Juste, Ra" uniqKey="Juste R">RA Juste</name></author><author><name sortKey="Anda, P" uniqKey="Anda P">P Anda</name></author><author><name sortKey="Garcia Perez, Al" uniqKey="Garcia Perez A">AL García-Pérez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yeruham, I" uniqKey="Yeruham I">I Yeruham</name></author><author><name sortKey="Hadani, A" uniqKey="Hadani A">A Hadani</name></author><author><name sortKey="Galker, F" uniqKey="Galker F">F Galker</name></author><author><name sortKey="Rosen, S" uniqKey="Rosen S">S Rosen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Norval, Rai" uniqKey="Norval R">RAI Norval</name></author><author><name sortKey="Perry, Bd" uniqKey="Perry B">BD Perry</name></author><author><name sortKey="Young, As" uniqKey="Young A">AS Young</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dalgliesh, Rj" uniqKey="Dalgliesh R">RJ Dalgliesh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wikel, Sk" uniqKey="Wikel S">SK Wikel</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article" xml:lang="en"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Parasit Vectors</journal-id><journal-id journal-id-type="iso-abbrev">Parasit Vectors</journal-id><journal-title-group><journal-title>Parasites & Vectors</journal-title></journal-title-group><issn pub-type="epub">1756-3305</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24499621</article-id><article-id pub-id-type="pmc">3849078</article-id><article-id pub-id-type="publisher-id">1756-3305-6-277</article-id><article-id pub-id-type="doi">10.1186/1756-3305-6-277</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group></article-categories><title-group><article-title>Assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex DNA bead-based suspension array</article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Ros-García</surname><given-names>Amaia</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>aros@neiker.net</email></contrib><contrib contrib-type="author" id="A2"><name><surname>Barandika</surname><given-names>Jesús F</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>jbarandika@neiker.net</email></contrib><contrib contrib-type="author" id="A3"><name><surname>García-Pérez</surname><given-names>Ana L</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>agarcia@neiker.net</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Juste</surname><given-names>Ramón A</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>rjuste@neiker.net</email></contrib><contrib contrib-type="author" corresp="yes" id="A5"><name><surname>Hurtado</surname><given-names>Ana</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>ahurtado@neiker.net</email></contrib></contrib-group><aff id="I1"><label>1</label>Department of Animal Health, NEIKER - Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga 1, Derio, Bizkaia 48160, Spain</aff><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>24</day><month>9</month><year>2013</year></pub-date><volume>6</volume><fpage>277</fpage><lpage>277</lpage><history><date date-type="received"><day>2</day><month>7</month><year>2013</year></date><date date-type="accepted"><day>17</day><month>9</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013 Ros-García et al.; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>Ros-García et al.; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.parasitesandvectors.com/content/6/1/277"></self-uri><abstract><sec><title>Background</title><p>Piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. Detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. The purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the Basque Country (northern Spain).</p></sec><sec><title>Methods</title><p>A DNA bead-based suspension array using the Luminex® xMAP technology that included a generic <italic>Theileria</italic>-<italic>Babesia</italic> control probe, 6 species-specific probes, and an internal control probe was developed to detect and identify piroplasms that infect sheep. To monitor piroplasm infection in clinically healthy sheep from 4 flocks that share communal mountain pastures, blood samples were collected during 2 grazing seasons.</p></sec><sec><title>Results</title><p>Piroplasms were detected in 48% (214/446) of blood samples, nearly half of them (49.1%, 105/214) as mixed infections. Five different piroplasms were identified: <italic>Theileria</italic> sp. OT3 in 34.8% of the samples, <italic>Theileria ovis</italic> in 20.9%, and at lower prevalences <italic>Babesia motasi</italic> (12.3%), <italic>Theileria luwenshuni</italic>/OT1 (10.5%) and <italic>Babesia ovis</italic> (6.3%). Despite differences among flocks associated to differences in management, an increasing trend in the incidence of piroplasm infection with increasing age of animals after increased tick exposure was observed. This increment could be attributed to continued re-infection associated with re-exposure to ticks at grazing. Ticks were collected from animals (4 species) and vegetation (8 species), and associations between tick abundance seasonality and risk of infection with the different piroplasms were established.</p></sec><sec><title>Conclusion</title><p>The multiplex Luminex® xMAP procedure is a rapid and high throughput technique that provided highly specific and sensitive identification of single and mixed piroplasm infections in blood of sheep carriers. This study confirmed a situation of endemic stability for piroplasm infection in the region, where infection is present in the absence of clinical signs, and mountain grazing allows for sufficient inoculation rates to maintain such situation.</p></sec></abstract><kwd-group><kwd><italic>Babesia</italic></kwd><kwd><italic>Theileria</italic></kwd><kwd>Luminex</kwd><kwd>Suspension microarray</kwd><kwd>Sheep</kwd><kwd>RLB</kwd><kwd>Tick-borne pathogens</kwd><kwd>Piroplasmosis</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Espagne</li></country><region><li>Pays basque</li></region></list><tree><country name="Espagne"><region name="Pays basque"><name sortKey="Ros Garcia, Amaia" sort="Ros Garcia, Amaia" uniqKey="Ros Garcia A" first="Amaia" last="Ros-García">Amaia Ros-García</name></region><name sortKey="Barandika, Jesus F" sort="Barandika, Jesus F" uniqKey="Barandika J" first="Jesús F" last="Barandika">Jesús F. Barandika</name><name sortKey="Garcia Perez, Ana L" sort="Garcia Perez, Ana L" uniqKey="Garcia Perez A" first="Ana L" last="García-Pérez">Ana L. García-Pérez</name><name sortKey="Hurtado, Ana" sort="Hurtado, Ana" uniqKey="Hurtado A" first="Ana" last="Hurtado">Ana Hurtado</name><name sortKey="Juste, Ram N A" sort="Juste, Ram N A" uniqKey="Juste R" first="Ram N A" last="Juste">Ram N A. Juste</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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