Transient Protein Expression by Agroinfiltration in Lettuce.
Identifieur interne : 000318 ( Main/Corpus ); précédent : 000317; suivant : 000319Transient Protein Expression by Agroinfiltration in Lettuce.
Auteurs : Qiang Chen ; Matthew Dent ; Jonathan Hurtado ; Jake Stahnke ; Alyssa Mcnulty ; Kahlin Leuzinger ; Huafang LaiSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical : Recombinant Proteins.
- genetics : Agrobacterium tumefaciens, Lettuce.
- metabolism : Lettuce.
- methods : Genetic Engineering.
- Gene Expression, Genetic Vectors, Plants, Genetically Modified, Transformation, Genetic.
Abstract
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.
DOI: 10.1007/978-1-4939-3289-4_4
PubMed: 26614281
Links to Exploration step
pubmed:26614281Le document en format XML
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<term>Gene Expression (MeSH)</term>
<term>Genetic Engineering (methods)</term>
<term>Genetic Vectors (MeSH)</term>
<term>Lettuce (genetics)</term>
<term>Lettuce (metabolism)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Recombinant Proteins (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
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<front><div type="abstract" xml:lang="en">Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.</div>
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<Abstract><AbstractText>Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.</AbstractText>
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<Keyword MajorTopicYN="N">Plant biology</Keyword>
<Keyword MajorTopicYN="N">Plant-made pharmaceutics</Keyword>
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