Transient Protein Expression by Agroinfiltration in Lettuce.
Identifieur interne : 000318 ( Main/Corpus ); précédent : 000317; suivant : 000319Transient Protein Expression by Agroinfiltration in Lettuce.
Auteurs : Qiang Chen ; Matthew Dent ; Jonathan Hurtado ; Jake Stahnke ; Alyssa Mcnulty ; Kahlin Leuzinger ; Huafang LaiSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical : Recombinant Proteins.
- genetics : Agrobacterium tumefaciens, Lettuce.
- metabolism : Lettuce.
- methods : Genetic Engineering.
- Gene Expression, Genetic Vectors, Plants, Genetically Modified, Transformation, Genetic.
Abstract
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.
DOI: 10.1007/978-1-4939-3289-4_4
PubMed: 26614281
Links to Exploration step
pubmed:26614281Le document en format XML
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Huafang.lai@asu.edu.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26614281</idno><idno type="pmid">26614281</idno><idno type="doi">10.1007/978-1-4939-3289-4_4</idno><idno type="wicri:Area/Main/Corpus">000318</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000318</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transient Protein Expression by Agroinfiltration in Lettuce.</title><author><name sortKey="Chen, Qiang" sort="Chen, Qiang" uniqKey="Chen Q" first="Qiang" last="Chen">Qiang Chen</name><affiliation><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</nlm:affiliation></affiliation></author><author><name sortKey="Dent, Matthew" sort="Dent, Matthew" uniqKey="Dent M" first="Matthew" last="Dent">Matthew Dent</name><affiliation><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Hurtado, Jonathan" sort="Hurtado, Jonathan" uniqKey="Hurtado J" first="Jonathan" last="Hurtado">Jonathan Hurtado</name><affiliation><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Stahnke, Jake" sort="Stahnke, Jake" uniqKey="Stahnke J" first="Jake" last="Stahnke">Jake Stahnke</name><affiliation><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Mcnulty, Alyssa" sort="Mcnulty, Alyssa" uniqKey="Mcnulty A" first="Alyssa" last="Mcnulty">Alyssa Mcnulty</name><affiliation><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Leuzinger, Kahlin" sort="Leuzinger, Kahlin" uniqKey="Leuzinger K" first="Kahlin" last="Leuzinger">Kahlin Leuzinger</name><affiliation><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lai, Huafang" sort="Lai, Huafang" uniqKey="Lai H" first="Huafang" last="Lai">Huafang Lai</name><affiliation><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. Huafang.lai@asu.edu.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Gene Expression (MeSH)</term><term>Genetic Engineering (methods)</term><term>Genetic Vectors (MeSH)</term><term>Lettuce (genetics)</term><term>Lettuce (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Recombinant Proteins (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Lettuce</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lettuce</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Engineering</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Genetic Vectors</term><term>Plants, Genetically Modified</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26614281</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>18</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1385</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Transient Protein Expression by Agroinfiltration in Lettuce.</ArticleTitle><Pagination><MedlinePgn>55-67</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-3289-4_4</ELocationID><Abstract><AbstractText>Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dent</LastName><ForeName>Matthew</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hurtado</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stahnke</LastName><ForeName>Jake</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McNulty</LastName><ForeName>Alyssa</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leuzinger</LastName><ForeName>Kahlin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lai</LastName><ForeName>Huafang</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. Huafang.lai@asu.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="Y">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018545" MajorTopicYN="N">Lettuce</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="Y">Recombinant Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium tumefaciens</Keyword><Keyword MajorTopicYN="N">Agroinfiltration</Keyword><Keyword MajorTopicYN="N">Antibodies</Keyword><Keyword MajorTopicYN="N">Biotechnology</Keyword><Keyword MajorTopicYN="N">Large-scale production</Keyword><Keyword MajorTopicYN="N">Lettuce</Keyword><Keyword MajorTopicYN="N">Plant biology</Keyword><Keyword MajorTopicYN="N">Plant-made pharmaceutics</Keyword><Keyword MajorTopicYN="N">Recombinant protein</Keyword><Keyword MajorTopicYN="N">Transient expression</Keyword><Keyword MajorTopicYN="N">Vaccines</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>11</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>11</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26614281</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-3289-4_4</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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