Contributions of adhesive proteins to the cellular and bacterial response to surfaces treated with bioactive polymers: case of poly(sodium styrene sulfonate) grafted titanium surfaces.
Identifieur interne : 002719 ( PubMed/Corpus ); précédent : 002718; suivant : 002720Contributions of adhesive proteins to the cellular and bacterial response to surfaces treated with bioactive polymers: case of poly(sodium styrene sulfonate) grafted titanium surfaces.
Auteurs : Helena P. Felgueiras ; Ines Ben Aissa ; Margaret D M. Evans ; Véronique MigonneySource :
- Journal of materials science. Materials in medicine [ 1573-4838 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Bacterial Proteins, Polymers, Titanium.
- physiology : Staphylococcus aureus.
- 3T3 Cells, Animals, Bacterial Adhesion, Cell Differentiation, Cell Proliferation, Mice, Protein Binding, Spectroscopy, Fourier Transform Infrared, Surface Properties.
Abstract
The research developed on functionalized model or prosthetic surfaces with bioactive polymers has raised the possibility to modulate and/or control the biological in vitro and in vivo responses to synthetic biomaterials. The mechanisms underlying the bioactivity exhibited by sulfonated groups on surfaces involves both selective adsorption and conformational changes of adsorbed proteins. Indeed, surfaces functionalized by grafting poly(sodium styrene sulfonate) [poly(NaSS)] modulate the cellular and bacterial response by inducing specific interactions with fibronectin (Fn). Once implanted, a biomaterial surface is exposed to a milieu of many proteins that compete for the surface which dictates the subsequent biological response. Once understood, this can be controlled by dictating exposure of active binding sites. In this in vitro study, we report the influence of binary mixtures of proteins [albumin (BSA), Fn and collagen type I (Col I)] adsorbed on poly(NaSS) grafted Ti6Al4V on the adhesion and differentiation of MC3T3-E1 osteoblast-like cells and the adhesion and proliferation of Staphylococcus aureus (S. aureus). Outcomes showed that poly(NaSS) stimulated cell spreading, attachment strength, differentiation and mineralization, whatever the nature of protein provided at the interface compared with ungrafted Ti6Al4V (control). While in competition, Fn and Col I were capable of prevailing over BSA. Fn played an important role in the early interactions of the cells with the surface, while Col I was responsible for increased alkaline phosphatase, calcium and phosphate productions associated with differentiation. Poly(NaSS) grafted surfaces decreased the adhesion of S. aureus and the presence of Fn on these chemically altered surfaces increased bacterial resistance ≈70% compared to the ungrafted Ti6Al4V. Overall, our study showed that poly(NaSS) grafted Ti6Al4V selectively adsorbed proteins (particularly Fn) promoting the adhesion and differentiation of osteoblast-like cells while reducing bacterial adhesion to create a bioactive surface with potential for orthopaedic applications.
DOI: 10.1007/s10856-015-5596-y
PubMed: 26449451
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pubmed:26449451Le document en format XML
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Felgueiras</name><affiliation><nlm:affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France.</nlm:affiliation></affiliation></author><author><name sortKey="Aissa, Ines Ben" sort="Aissa, Ines Ben" uniqKey="Aissa I" first="Ines Ben" last="Aissa">Ines Ben Aissa</name><affiliation><nlm:affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France.</nlm:affiliation></affiliation></author><author><name sortKey="Evans, Margaret D M" sort="Evans, Margaret D M" uniqKey="Evans M" first="Margaret D M" last="Evans">Margaret D M. Evans</name><affiliation><nlm:affiliation>CSIRO Biomedical Materials Program, 11 Julius Avenue, North Ride, Sydney, NSW, 2113, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Migonney, Veronique" sort="Migonney, Veronique" uniqKey="Migonney V" first="Véronique" last="Migonney">Véronique Migonney</name><affiliation><nlm:affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France. veronique.migonney@univ-paris13.fr.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26449451</idno><idno type="pmid">26449451</idno><idno type="doi">10.1007/s10856-015-5596-y</idno><idno type="wicri:Area/PubMed/Corpus">002719</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002719</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Contributions of adhesive proteins to the cellular and bacterial response to surfaces treated with bioactive polymers: case of poly(sodium styrene sulfonate) grafted titanium surfaces.</title><author><name sortKey="Felgueiras, Helena P" sort="Felgueiras, Helena P" uniqKey="Felgueiras H" first="Helena P" last="Felgueiras">Helena P. Felgueiras</name><affiliation><nlm:affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France.</nlm:affiliation></affiliation></author><author><name sortKey="Aissa, Ines Ben" sort="Aissa, Ines Ben" uniqKey="Aissa I" first="Ines Ben" last="Aissa">Ines Ben Aissa</name><affiliation><nlm:affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France.</nlm:affiliation></affiliation></author><author><name sortKey="Evans, Margaret D M" sort="Evans, Margaret D M" uniqKey="Evans M" first="Margaret D M" last="Evans">Margaret D M. Evans</name><affiliation><nlm:affiliation>CSIRO Biomedical Materials Program, 11 Julius Avenue, North Ride, Sydney, NSW, 2113, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Migonney, Veronique" sort="Migonney, Veronique" uniqKey="Migonney V" first="Véronique" last="Migonney">Véronique Migonney</name><affiliation><nlm:affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France. veronique.migonney@univ-paris13.fr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of materials science. Materials in medicine</title><idno type="eISSN">1573-4838</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3T3 Cells</term><term>Animals</term><term>Bacterial Adhesion</term><term>Bacterial Proteins (chemistry)</term><term>Cell Differentiation</term><term>Cell Proliferation</term><term>Mice</term><term>Polymers (chemistry)</term><term>Protein Binding</term><term>Spectroscopy, Fourier Transform Infrared</term><term>Staphylococcus aureus (physiology)</term><term>Surface Properties</term><term>Titanium (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bacterial Proteins</term><term>Polymers</term><term>Titanium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>3T3 Cells</term><term>Animals</term><term>Bacterial Adhesion</term><term>Cell Differentiation</term><term>Cell Proliferation</term><term>Mice</term><term>Protein Binding</term><term>Spectroscopy, Fourier Transform Infrared</term><term>Surface Properties</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The research developed on functionalized model or prosthetic surfaces with bioactive polymers has raised the possibility to modulate and/or control the biological in vitro and in vivo responses to synthetic biomaterials. The mechanisms underlying the bioactivity exhibited by sulfonated groups on surfaces involves both selective adsorption and conformational changes of adsorbed proteins. Indeed, surfaces functionalized by grafting poly(sodium styrene sulfonate) [poly(NaSS)] modulate the cellular and bacterial response by inducing specific interactions with fibronectin (Fn). Once implanted, a biomaterial surface is exposed to a milieu of many proteins that compete for the surface which dictates the subsequent biological response. Once understood, this can be controlled by dictating exposure of active binding sites. In this in vitro study, we report the influence of binary mixtures of proteins [albumin (BSA), Fn and collagen type I (Col I)] adsorbed on poly(NaSS) grafted Ti6Al4V on the adhesion and differentiation of MC3T3-E1 osteoblast-like cells and the adhesion and proliferation of Staphylococcus aureus (S. aureus). Outcomes showed that poly(NaSS) stimulated cell spreading, attachment strength, differentiation and mineralization, whatever the nature of protein provided at the interface compared with ungrafted Ti6Al4V (control). While in competition, Fn and Col I were capable of prevailing over BSA. Fn played an important role in the early interactions of the cells with the surface, while Col I was responsible for increased alkaline phosphatase, calcium and phosphate productions associated with differentiation. Poly(NaSS) grafted surfaces decreased the adhesion of S. aureus and the presence of Fn on these chemically altered surfaces increased bacterial resistance ≈70% compared to the ungrafted Ti6Al4V. Overall, our study showed that poly(NaSS) grafted Ti6Al4V selectively adsorbed proteins (particularly Fn) promoting the adhesion and differentiation of osteoblast-like cells while reducing bacterial adhesion to create a bioactive surface with potential for orthopaedic applications.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26449451</PMID><DateCreated><Year>2015</Year><Month>10</Month><Day>09</Day></DateCreated><DateCompleted><Year>2016</Year><Month>08</Month><Day>26</Day></DateCompleted><DateRevised><Year>2017</Year><Month>10</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-4838</ISSN><JournalIssue CitedMedium="Internet"><Volume>26</Volume><Issue>11</Issue><PubDate><Year>2015</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of materials science. Materials in medicine</Title><ISOAbbreviation>J Mater Sci Mater Med</ISOAbbreviation></Journal><ArticleTitle>Contributions of adhesive proteins to the cellular and bacterial response to surfaces treated with bioactive polymers: case of poly(sodium styrene sulfonate) grafted titanium surfaces.</ArticleTitle><Pagination><MedlinePgn>261</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10856-015-5596-y</ELocationID><Abstract><AbstractText>The research developed on functionalized model or prosthetic surfaces with bioactive polymers has raised the possibility to modulate and/or control the biological in vitro and in vivo responses to synthetic biomaterials. The mechanisms underlying the bioactivity exhibited by sulfonated groups on surfaces involves both selective adsorption and conformational changes of adsorbed proteins. Indeed, surfaces functionalized by grafting poly(sodium styrene sulfonate) [poly(NaSS)] modulate the cellular and bacterial response by inducing specific interactions with fibronectin (Fn). Once implanted, a biomaterial surface is exposed to a milieu of many proteins that compete for the surface which dictates the subsequent biological response. Once understood, this can be controlled by dictating exposure of active binding sites. In this in vitro study, we report the influence of binary mixtures of proteins [albumin (BSA), Fn and collagen type I (Col I)] adsorbed on poly(NaSS) grafted Ti6Al4V on the adhesion and differentiation of MC3T3-E1 osteoblast-like cells and the adhesion and proliferation of Staphylococcus aureus (S. aureus). Outcomes showed that poly(NaSS) stimulated cell spreading, attachment strength, differentiation and mineralization, whatever the nature of protein provided at the interface compared with ungrafted Ti6Al4V (control). While in competition, Fn and Col I were capable of prevailing over BSA. Fn played an important role in the early interactions of the cells with the surface, while Col I was responsible for increased alkaline phosphatase, calcium and phosphate productions associated with differentiation. Poly(NaSS) grafted surfaces decreased the adhesion of S. aureus and the presence of Fn on these chemically altered surfaces increased bacterial resistance ≈70% compared to the ungrafted Ti6Al4V. Overall, our study showed that poly(NaSS) grafted Ti6Al4V selectively adsorbed proteins (particularly Fn) promoting the adhesion and differentiation of osteoblast-like cells while reducing bacterial adhesion to create a bioactive surface with potential for orthopaedic applications.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Felgueiras</LastName><ForeName>Helena P</ForeName><Initials>HP</Initials><AffiliationInfo><Affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aissa</LastName><ForeName>Ines Ben</ForeName><Initials>IB</Initials><AffiliationInfo><Affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Evans</LastName><ForeName>Margaret D M</ForeName><Initials>MD</Initials><AffiliationInfo><Affiliation>CSIRO Biomedical Materials Program, 11 Julius Avenue, North Ride, Sydney, NSW, 2113, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Migonney</LastName><ForeName>Véronique</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France. veronique.migonney@univ-paris13.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>10</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Mater Sci Mater Med</MedlineTA><NlmUniqueID>9013087</NlmUniqueID><ISSNLinking>0957-4530</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011108">Polymers</NameOfSubstance></Chemical><Chemical><RegistryNumber>D1JT611TNE</RegistryNumber><NameOfSubstance UI="D014025">Titanium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Biomed Mater Res A. 2007 Dec 1;83(3):712-9</RefSource><PMID Version="1">17559125</PMID></CommentsCorrections><CommentsCorrections 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MajorTopicYN="N">3T3 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001422" MajorTopicYN="N">Bacterial Adhesion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011108" MajorTopicYN="N">Polymers</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein 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