Cellular Response to Linear and Branched Poly(acrylic acid).
Identifieur interne : 002683 ( PubMed/Corpus ); précédent : 002682; suivant : 002684Cellular Response to Linear and Branched Poly(acrylic acid).
Auteurs : Elizabeth G. Whitty ; Alison R. Maniego ; Sharon A. Bentwitch ; Yohann Guillaneuf ; Mark R. Jones ; Marianne Gaborieau ; Patrice CastignollesSource :
- Macromolecular bioscience [ 1616-5195 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Acrylic Resins, Blood Proteins.
- metabolism : Myoblasts, Skeletal.
- Animals, Cell Line, Tumor, Cell Survival, Mice.
Abstract
Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L(-1), showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.
DOI: 10.1002/mabi.201500153
PubMed: 26257305
Links to Exploration step
pubmed:26257305Le document en format XML
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Bentwitch</name><affiliation><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Guillaneuf, Yohann" sort="Guillaneuf, Yohann" uniqKey="Guillaneuf Y" first="Yohann" last="Guillaneuf">Yohann Guillaneuf</name><affiliation><nlm:affiliation>CNRS, Institut de Chimie Radicalaire, Aix-Marseille Université, UMR 7273, 13397, Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Jones, Mark R" sort="Jones, Mark R" uniqKey="Jones M" first="Mark R" last="Jones">Mark R. Jones</name><affiliation><nlm:affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Gaborieau, Marianne" sort="Gaborieau, Marianne" uniqKey="Gaborieau M" first="Marianne" last="Gaborieau">Marianne Gaborieau</name><affiliation><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.</nlm:affiliation></affiliation></author><author><name sortKey="Castignolles, Patrice" sort="Castignolles, Patrice" uniqKey="Castignolles P" first="Patrice" last="Castignolles">Patrice Castignolles</name><affiliation><nlm:affiliation>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26257305</idno><idno type="pmid">26257305</idno><idno type="doi">10.1002/mabi.201500153</idno><idno type="wicri:Area/PubMed/Corpus">002683</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002683</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cellular Response to Linear and Branched Poly(acrylic acid).</title><author><name sortKey="Whitty, Elizabeth G" sort="Whitty, Elizabeth G" uniqKey="Whitty E" first="Elizabeth G" last="Whitty">Elizabeth G. Whitty</name><affiliation><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Maniego, Alison R" sort="Maniego, Alison R" uniqKey="Maniego A" first="Alison R" last="Maniego">Alison R. Maniego</name><affiliation><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Bentwitch, Sharon A" sort="Bentwitch, Sharon A" uniqKey="Bentwitch S" first="Sharon A" last="Bentwitch">Sharon A. Bentwitch</name><affiliation><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Guillaneuf, Yohann" sort="Guillaneuf, Yohann" uniqKey="Guillaneuf Y" first="Yohann" last="Guillaneuf">Yohann Guillaneuf</name><affiliation><nlm:affiliation>CNRS, Institut de Chimie Radicalaire, Aix-Marseille Université, UMR 7273, 13397, Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Jones, Mark R" sort="Jones, Mark R" uniqKey="Jones M" first="Mark R" last="Jones">Mark R. Jones</name><affiliation><nlm:affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Gaborieau, Marianne" sort="Gaborieau, Marianne" uniqKey="Gaborieau M" first="Marianne" last="Gaborieau">Marianne Gaborieau</name><affiliation><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.</nlm:affiliation></affiliation></author><author><name sortKey="Castignolles, Patrice" sort="Castignolles, Patrice" uniqKey="Castignolles P" first="Patrice" last="Castignolles">Patrice Castignolles</name><affiliation><nlm:affiliation>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Macromolecular bioscience</title><idno type="eISSN">1616-5195</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrylic Resins (chemistry)</term><term>Animals</term><term>Blood Proteins (chemistry)</term><term>Cell Line, Tumor</term><term>Cell Survival</term><term>Mice</term><term>Myoblasts, Skeletal (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acrylic Resins</term><term>Blood Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Myoblasts, Skeletal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line, Tumor</term><term>Cell Survival</term><term>Mice</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L(-1), showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26257305</PMID><DateCreated><Year>2016</Year><Month>01</Month><Day>15</Day></DateCreated><DateCompleted><Year>2016</Year><Month>10</Month><Day>10</Day></DateCompleted><DateRevised><Year>2017</Year><Month>01</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1616-5195</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>12</Issue><PubDate><Year>2015</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Macromolecular bioscience</Title><ISOAbbreviation>Macromol Biosci</ISOAbbreviation></Journal><ArticleTitle>Cellular Response to Linear and Branched Poly(acrylic acid).</ArticleTitle><Pagination><MedlinePgn>1724-34</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/mabi.201500153</ELocationID><Abstract><AbstractText>Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L(-1), showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.</AbstractText><CopyrightInformation>© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Whitty</LastName><ForeName>Elizabeth G</ForeName><Initials>EG</Initials><AffiliationInfo><Affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maniego</LastName><ForeName>Alison R</ForeName><Initials>AR</Initials><AffiliationInfo><Affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bentwitch</LastName><ForeName>Sharon A</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guillaneuf</LastName><ForeName>Yohann</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>CNRS, Institut de Chimie Radicalaire, Aix-Marseille Université, UMR 7273, 13397, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jones</LastName><ForeName>Mark R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gaborieau</LastName><ForeName>Marianne</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Castignolles</LastName><ForeName>Patrice</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>08</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Macromol Biosci</MedlineTA><NlmUniqueID>101135941</NlmUniqueID><ISSNLinking>1616-5187</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000180">Acrylic Resins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001798">Blood Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>4Q93RCW27E</RegistryNumber><NameOfSubstance UI="C006903">carbopol 940</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000180" MajorTopicYN="N">Acrylic Resins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001798" MajorTopicYN="N">Blood Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032448" MajorTopicYN="N">Myoblasts, Skeletal</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MTT assay</Keyword><Keyword MajorTopicYN="N">capillary electrophoresis</Keyword><Keyword MajorTopicYN="N">cell</Keyword><Keyword MajorTopicYN="N">cytotoxicity</Keyword><Keyword MajorTopicYN="N">poly(acrylic acid)/poly(sodium acrylate)</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>04</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>06</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26257305</ArticleId><ArticleId 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