In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi?
Identifieur interne : 001A73 ( PubMed/Corpus ); précédent : 001A72; suivant : 001A74In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi?
Auteurs : Cécile Lepère ; Martin Ostrowski ; Manuela Hartmann ; Mikhail V. Zubkov ; David J. ScanlanSource :
- Environmental microbiology reports [ 1758-2229 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- genetics : Alveolata, Fungi.
- isolation & purification : Alveolata, Fungi.
- microbiology : Phytoplankton.
- parasitology : Phytoplankton.
- Atlantic Ocean, Biodiversity, Flow Cytometry, Host-Parasite Interactions, In Situ Hybridization, Fluorescence.
Abstract
Photosynthetic picoeukaryotes (PPEs) are important components of the marine picophytoplankton community playing a critical role in CO2 fixation but also as bacterivores, particularly in the oligotrophic gyres. Despite an increased interest in these organisms and an improved understanding of the genetic diversity of this group, we still know little of the environmental factors controlling the abundance of these organisms. Here, we investigated the quantitative importance of eukaryotic parasites in the free-living fraction as well as in associations with PPEs along a transect in the South Atlantic. Using tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH), we provide quantitative evidence of the occurrence of free-living fungi in open ocean marine systems, while the Perkinsozoa and Syndiniales parasites were not abundant in these waters. Using flow cytometric cell sorting of different PPE populations followed by a dual-labelled TSA-FISH approach, we also demonstrate fungal associations, potentially parasitic, occurring with both pico-Prymnesiophyceae and pico-Chrysophyceae. These data highlight the necessity for further work investigating the specific role of marine fungi as parasites of phytoplankton to improve understanding of carbon flow in marine ecosystems.
DOI: 10.1111/1758-2229.12339
PubMed: 26420747
Links to Exploration step
pubmed:26420747Le document en format XML
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Zubkov</name><affiliation><nlm:affiliation>National Oceanography Centre, Hampshire, SO14 3ZH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Scanlan, David J" sort="Scanlan, David J" uniqKey="Scanlan D" first="David J" last="Scanlan">David J. Scanlan</name><affiliation><nlm:affiliation>School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26420747</idno><idno type="pmid">26420747</idno><idno type="doi">10.1111/1758-2229.12339</idno><idno type="wicri:Area/PubMed/Corpus">001A73</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A73</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi?</title><author><name sortKey="Lepere, Cecile" sort="Lepere, Cecile" uniqKey="Lepere C" first="Cécile" last="Lepère">Cécile Lepère</name><affiliation><nlm:affiliation>School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Ostrowski, Martin" sort="Ostrowski, Martin" uniqKey="Ostrowski M" first="Martin" last="Ostrowski">Martin Ostrowski</name><affiliation><nlm:affiliation>Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, NSW, 2109, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Hartmann, Manuela" sort="Hartmann, Manuela" uniqKey="Hartmann M" first="Manuela" last="Hartmann">Manuela Hartmann</name><affiliation><nlm:affiliation>National Oceanography Centre, Hampshire, SO14 3ZH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Zubkov, Mikhail V" sort="Zubkov, Mikhail V" uniqKey="Zubkov M" first="Mikhail V" last="Zubkov">Mikhail V. Zubkov</name><affiliation><nlm:affiliation>National Oceanography Centre, Hampshire, SO14 3ZH, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Scanlan, David J" sort="Scanlan, David J" uniqKey="Scanlan D" first="David J" last="Scanlan">David J. Scanlan</name><affiliation><nlm:affiliation>School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Environmental microbiology reports</title><idno type="eISSN">1758-2229</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alveolata (genetics)</term><term>Alveolata (isolation & purification)</term><term>Atlantic Ocean</term><term>Biodiversity</term><term>Flow Cytometry</term><term>Fungi (genetics)</term><term>Fungi (isolation & purification)</term><term>Host-Parasite Interactions</term><term>In Situ Hybridization, Fluorescence</term><term>Phytoplankton (microbiology)</term><term>Phytoplankton (parasitology)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Alveolata</term><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Alveolata</term><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Phytoplankton</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Phytoplankton</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Atlantic Ocean</term><term>Biodiversity</term><term>Flow Cytometry</term><term>Host-Parasite Interactions</term><term>In Situ Hybridization, Fluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Photosynthetic picoeukaryotes (PPEs) are important components of the marine picophytoplankton community playing a critical role in CO2 fixation but also as bacterivores, particularly in the oligotrophic gyres. Despite an increased interest in these organisms and an improved understanding of the genetic diversity of this group, we still know little of the environmental factors controlling the abundance of these organisms. Here, we investigated the quantitative importance of eukaryotic parasites in the free-living fraction as well as in associations with PPEs along a transect in the South Atlantic. Using tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH), we provide quantitative evidence of the occurrence of free-living fungi in open ocean marine systems, while the Perkinsozoa and Syndiniales parasites were not abundant in these waters. Using flow cytometric cell sorting of different PPE populations followed by a dual-labelled TSA-FISH approach, we also demonstrate fungal associations, potentially parasitic, occurring with both pico-Prymnesiophyceae and pico-Chrysophyceae. These data highlight the necessity for further work investigating the specific role of marine fungi as parasites of phytoplankton to improve understanding of carbon flow in marine ecosystems.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26420747</PMID><DateCreated><Year>2016</Year><Month>07</Month><Day>05</Day></DateCreated><DateCompleted><Year>2017</Year><Month>08</Month><Day>29</Day></DateCompleted><DateRevised><Year>2017</Year><Month>09</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>4</Issue><PubDate><Year>2016</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Environmental microbiology reports</Title><ISOAbbreviation>Environ Microbiol Rep</ISOAbbreviation></Journal><ArticleTitle>In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi?</ArticleTitle><Pagination><MedlinePgn>445-51</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/1758-2229.12339</ELocationID><Abstract><AbstractText>Photosynthetic picoeukaryotes (PPEs) are important components of the marine picophytoplankton community playing a critical role in CO2 fixation but also as bacterivores, particularly in the oligotrophic gyres. Despite an increased interest in these organisms and an improved understanding of the genetic diversity of this group, we still know little of the environmental factors controlling the abundance of these organisms. Here, we investigated the quantitative importance of eukaryotic parasites in the free-living fraction as well as in associations with PPEs along a transect in the South Atlantic. Using tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH), we provide quantitative evidence of the occurrence of free-living fungi in open ocean marine systems, while the Perkinsozoa and Syndiniales parasites were not abundant in these waters. Using flow cytometric cell sorting of different PPE populations followed by a dual-labelled TSA-FISH approach, we also demonstrate fungal associations, potentially parasitic, occurring with both pico-Prymnesiophyceae and pico-Chrysophyceae. These data highlight the necessity for further work investigating the specific role of marine fungi as parasites of phytoplankton to improve understanding of carbon flow in marine ecosystems.</AbstractText><CopyrightInformation>© 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lepère</LastName><ForeName>Cécile</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université Clermont Auvergne, Université Blaise Pascal, Laboratoire 'Microorganismes : Génome et Environnement', BP 10448, Clermont-Ferrand, F-63000, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>CNRS, UMR 6023, LMGE, Aubière, F-63171, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ostrowski</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, NSW, 2109, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hartmann</LastName><ForeName>Manuela</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>National Oceanography Centre, Hampshire, SO14 3ZH, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zubkov</LastName><ForeName>Mikhail V</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>National Oceanography Centre, Hampshire, SO14 3ZH, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scanlan</LastName><ForeName>David J</ForeName><Initials>DJ</Initials><AffiliationInfo><Affiliation>School of Life Sciences, University of Warwick, Coventry, CV4 7AL, 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