Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.
Identifieur interne : 001153 ( PubMed/Corpus ); précédent : 001152; suivant : 001154Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.
Auteurs : Blandine Rougemont ; Sébastien Bontemps Gallo ; Sophie Ayciriex ; Romain Carrière ; Hubert Hondermarck ; Jean Marie Lacroix ; J C Yves Le Blanc ; Jérôme LemoineSource :
- Analytical chemistry [ 1520-6882 ] ; 2017.
Abstract
Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.
DOI: 10.1021/acs.analchem.6b03201
PubMed: 28029036
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.</title><author><name sortKey="Rougemont, Blandine" sort="Rougemont, Blandine" uniqKey="Rougemont B" first="Blandine" last="Rougemont">Blandine Rougemont</name><affiliation><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation></affiliation></author><author><name sortKey="Bontemps Gallo, Sebastien" sort="Bontemps Gallo, Sebastien" uniqKey="Bontemps Gallo S" first="Sébastien" last="Bontemps Gallo">Sébastien Bontemps Gallo</name><affiliation><nlm:affiliation>Université de Lille, CNRS, UMR 8576 , Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Ayciriex, Sophie" sort="Ayciriex, Sophie" uniqKey="Ayciriex S" first="Sophie" last="Ayciriex">Sophie Ayciriex</name><affiliation><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation></affiliation></author><author><name sortKey="Carriere, Romain" sort="Carriere, Romain" uniqKey="Carriere R" first="Romain" last="Carrière">Romain Carrière</name><affiliation><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation></affiliation></author><author><name sortKey="Hondermarck, Hubert" sort="Hondermarck, Hubert" uniqKey="Hondermarck H" first="Hubert" last="Hondermarck">Hubert Hondermarck</name><affiliation><nlm:affiliation>School of Biomedical Sciences and Pharmacy and Hunter Medical Research Institute, University of Newcastle , Callaghan, NSW 2308, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Lacroix, Jean Marie" sort="Lacroix, Jean Marie" uniqKey="Lacroix J" first="Jean Marie" last="Lacroix">Jean Marie Lacroix</name><affiliation><nlm:affiliation>Université de Lille, CNRS, UMR 8576 , Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Le Blanc, J C Yves" sort="Le Blanc, J C Yves" uniqKey="Le Blanc J" first="J C Yves" last="Le Blanc">J C Yves Le Blanc</name><affiliation><nlm:affiliation>SCIEX, 71 Four Valley Drive, Concord, Ontario L4K 4 V8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Lemoine, Jerome" sort="Lemoine, Jerome" uniqKey="Lemoine J" first="Jérôme" last="Lemoine">Jérôme Lemoine</name><affiliation><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28029036</idno><idno type="pmid">28029036</idno><idno type="doi">10.1021/acs.analchem.6b03201</idno><idno type="wicri:Area/PubMed/Corpus">001153</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001153</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.</title><author><name sortKey="Rougemont, Blandine" sort="Rougemont, Blandine" uniqKey="Rougemont B" first="Blandine" last="Rougemont">Blandine Rougemont</name><affiliation><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation></affiliation></author><author><name sortKey="Bontemps Gallo, Sebastien" sort="Bontemps Gallo, Sebastien" uniqKey="Bontemps Gallo S" first="Sébastien" last="Bontemps Gallo">Sébastien Bontemps Gallo</name><affiliation><nlm:affiliation>Université de Lille, CNRS, UMR 8576 , Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Ayciriex, Sophie" sort="Ayciriex, Sophie" uniqKey="Ayciriex S" first="Sophie" last="Ayciriex">Sophie Ayciriex</name><affiliation><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation></affiliation></author><author><name sortKey="Carriere, Romain" sort="Carriere, Romain" uniqKey="Carriere R" first="Romain" last="Carrière">Romain Carrière</name><affiliation><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation></affiliation></author><author><name sortKey="Hondermarck, Hubert" sort="Hondermarck, Hubert" uniqKey="Hondermarck H" first="Hubert" last="Hondermarck">Hubert Hondermarck</name><affiliation><nlm:affiliation>School of Biomedical Sciences and Pharmacy and Hunter Medical Research Institute, University of Newcastle , Callaghan, NSW 2308, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Lacroix, Jean Marie" sort="Lacroix, Jean Marie" uniqKey="Lacroix J" first="Jean Marie" last="Lacroix">Jean Marie Lacroix</name><affiliation><nlm:affiliation>Université de Lille, CNRS, UMR 8576 , Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Le Blanc, J C Yves" sort="Le Blanc, J C Yves" uniqKey="Le Blanc J" first="J C Yves" last="Le Blanc">J C Yves Le Blanc</name><affiliation><nlm:affiliation>SCIEX, 71 Four Valley Drive, Concord, Ontario L4K 4 V8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Lemoine, Jerome" sort="Lemoine, Jerome" uniqKey="Lemoine J" first="Jérôme" last="Lemoine">Jérôme Lemoine</name><affiliation><nlm:affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Analytical chemistry</title><idno type="eISSN">1520-6882</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">28029036</PMID><DateCreated><Year>2016</Year><Month>12</Month><Day>28</Day></DateCreated><DateRevised><Year>2017</Year><Month>02</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6882</ISSN><JournalIssue CitedMedium="Internet"><Volume>89</Volume><Issue>3</Issue><PubDate><Year>2017</Year><Month>Feb</Month><Day>07</Day></PubDate></JournalIssue><Title>Analytical chemistry</Title><ISOAbbreviation>Anal. Chem.</ISOAbbreviation></Journal><ArticleTitle>Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.</ArticleTitle><Pagination><MedlinePgn>1421-1426</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.analchem.6b03201</ELocationID><Abstract><AbstractText>Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rougemont</LastName><ForeName>Blandine</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bontemps Gallo</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Université de Lille, CNRS, UMR 8576 , Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ayciriex</LastName><ForeName>Sophie</ForeName><Initials>S</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-4813-5900</Identifier><AffiliationInfo><Affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carrière</LastName><ForeName>Romain</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hondermarck</LastName><ForeName>Hubert</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>School of Biomedical Sciences and Pharmacy and Hunter Medical Research Institute, University of Newcastle , Callaghan, NSW 2308, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lacroix</LastName><ForeName>Jean Marie</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Université de Lille, CNRS, UMR 8576 , Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Le Blanc</LastName><ForeName>J C Yves</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>SCIEX, 71 Four Valley Drive, Concord, Ontario L4K 4 V8, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lemoine</LastName><ForeName>Jérôme</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-5497-6880</Identifier><AffiliationInfo><Affiliation>Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Chem</MedlineTA><NlmUniqueID>0370536</NlmUniqueID><ISSNLinking>0003-2700</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>12</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>12</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28029036</ArticleId><ArticleId IdType="doi">10.1021/acs.analchem.6b03201</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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