Analytical Comparison of In Vitro-Spiked Human Serum and Plasma for PCR-Based Detection of Aspergillus fumigatus DNA: a Study by the European Aspergillus PCR Initiative.
Identifieur interne : 002F42 ( PubMed/Checkpoint ); précédent : 002F41; suivant : 002F43Analytical Comparison of In Vitro-Spiked Human Serum and Plasma for PCR-Based Detection of Aspergillus fumigatus DNA: a Study by the European Aspergillus PCR Initiative.
Auteurs : Juergen Loeffler [Allemagne] ; Carlo Mengoli [Italie] ; Jan Springer [Allemagne] ; Stéphane Bretagne [France] ; Manuel Cuenca-Estrella [Espagne] ; Lena Klingspor [Suède] ; Katrien Lagrou [Belgique] ; Willem J G. Melchers [Pays-Bas] ; C Oliver Morton [Australie] ; Rosemary A. Barnes [Royaume-Uni] ; J Peter Donnelly [Pays-Bas] ; P Lewis White [Royaume-Uni]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
Descripteurs français
- KwdFr :
- ADN fongique (sang), Aspergillose (diagnostic), Aspergillose (microbiologie), Aspergillus fumigatus (génétique), Aspergillus fumigatus (isolement et purification), Facteurs temps, Humains, Modèles biologiques, Plasma sanguin (microbiologie), Prélèvement biologique (), Réaction de polymérisation en chaîne (), Sensibilité et spécificité, Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Aspergillose.
- génétique : Aspergillus fumigatus.
- isolement et purification : Aspergillus fumigatus.
- microbiologie : Aspergillose, Plasma sanguin.
- sang : ADN fongique.
- Facteurs temps, Humains, Modèles biologiques, Prélèvement biologique, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Techniques de diagnostic moléculaire.
English descriptors
- KwdEn :
- Aspergillosis (diagnosis), Aspergillosis (microbiology), Aspergillus fumigatus (genetics), Aspergillus fumigatus (isolation & purification), DNA, Fungal (blood), Humans, Models, Biological, Molecular Diagnostic Techniques (methods), Plasma (microbiology), Polymerase Chain Reaction (methods), Sensitivity and Specificity, Specimen Handling (methods), Time Factors.
- MESH :
- chemical , blood : DNA, Fungal.
- diagnosis : Aspergillosis.
- genetics : Aspergillus fumigatus.
- isolation & purification : Aspergillus fumigatus.
- methods : Molecular Diagnostic Techniques, Polymerase Chain Reaction, Specimen Handling.
- microbiology : Aspergillosis, Plasma.
- Humans, Models, Biological, Sensitivity and Specificity, Time Factors.
Abstract
The use of serum or plasma for Aspergillus PCR testing facilitates automated and standardized technology. Recommendations for serum testing are available, and while serum and plasma are regularly considered interchangeable for use in fungal diagnostics, differences in galactomannan enzyme immunoassay (GM-EIA) performance have been reported and are attributed to clot formation. Therefore, it is important to assess plasma PCR testing to determine if previous recommendations for serum are applicable and also to compare analytical performance with that of serum PCR. Molecular methods testing serum and plasma were compared through multicenter distribution of quality control panels, with additional studies to investigate the effect of clot formation and blood fractionation on DNA availability. Analytical sensitivity and time to positivity (TTP) were compared, and a regression analysis was performed to identify variables that enhanced plasma PCR performance. When testing plasma, sample volume, preextraction-to-postextraction volume ratio, PCR volume, duplicate testing, and the use of an internal control for PCR were positively associated with performance. When whole-blood samples were spiked and then fractionated, the analytical sensitivity and TTP were superior when testing plasma. Centrifugation had no effect on DNA availability, whereas the presence of clot material significantly lowered the concentration (P = 0.028). Technically, there are no major differences in the molecular processing of serum and plasma, but the formation of clot material potentially reduces available DNA in serum. During disease, Aspergillus DNA burdens in blood are often at the limits of PCR performance. Using plasma might improve performance while maintaining the methodological simplicity of serum testing.
DOI: 10.1128/JCM.00906-15
PubMed: 26085614
Affiliations:
- Allemagne, Australie, Belgique, Espagne, France, Italie, Pays-Bas, Royaume-Uni, Suède
- Bavière, Communauté de Madrid, District de Basse-Franconie, Gueldre, Svealand, Île-de-France
- Madrid, Nimègue, Paris, Stockholm, Wurtzbourg
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pubmed:26085614Le document en format XML
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wicri:step="PubMed">002895</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Analytical Comparison of In Vitro-Spiked Human Serum and Plasma for PCR-Based Detection of Aspergillus fumigatus DNA: a Study by the European Aspergillus PCR Initiative.</title><author><name sortKey="Loeffler, Juergen" sort="Loeffler, Juergen" uniqKey="Loeffler J" first="Juergen" last="Loeffler">Juergen Loeffler</name><affiliation wicri:level="3"><nlm:affiliation>Würzburg University, Würzburg, Germany loeffler_j@ukw.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Würzburg University, Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Mengoli, Carlo" sort="Mengoli, Carlo" uniqKey="Mengoli C" first="Carlo" last="Mengoli">Carlo Mengoli</name><affiliation wicri:level="1"><nlm:affiliation>University of Padua, Padua, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>University of Padua, Padua</wicri:regionArea><wicri:noRegion>Padua</wicri:noRegion></affiliation></author><author><name sortKey="Springer, Jan" sort="Springer, Jan" uniqKey="Springer J" first="Jan" last="Springer">Jan Springer</name><affiliation wicri:level="3"><nlm:affiliation>Würzburg University, Würzburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Würzburg University, Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Bretagne, Stephane" sort="Bretagne, Stephane" uniqKey="Bretagne S" first="Stéphane" last="Bretagne">Stéphane Bretagne</name><affiliation wicri:level="3"><nlm:affiliation>Paris Diderot University, Saint Louis Hospital-APHP, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Paris Diderot University, Saint Louis Hospital-APHP, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Cuenca Estrella, Manuel" sort="Cuenca Estrella, Manuel" uniqKey="Cuenca Estrella M" first="Manuel" last="Cuenca-Estrella">Manuel Cuenca-Estrella</name><affiliation wicri:level="3"><nlm:affiliation>Spanish National Centre for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Spanish National Centre for Microbiology, Instituto de Salud Carlos III, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Klingspor, Lena" sort="Klingspor, Lena" uniqKey="Klingspor L" first="Lena" last="Klingspor">Lena Klingspor</name><affiliation wicri:level="3"><nlm:affiliation>Karolinska University Hospital, Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Karolinska University Hospital, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Lagrou, Katrien" sort="Lagrou, Katrien" uniqKey="Lagrou K" first="Katrien" last="Lagrou">Katrien Lagrou</name><affiliation wicri:level="1"><nlm:affiliation>National Reference Center for Mycosis, University Hospitals Leuven, Leuven, Belgium Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>National Reference Center for Mycosis, University Hospitals Leuven, Leuven, Belgium Department of Microbiology and Immunology, KU Leuven, Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation></author><author><name sortKey="Melchers, Willem J G" sort="Melchers, Willem J G" uniqKey="Melchers W" first="Willem J G" last="Melchers">Willem J G. Melchers</name><affiliation wicri:level="3"><nlm:affiliation>Radboud University Medical Centre, Nijmegen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Radboud University Medical Centre, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Morton, C Oliver" sort="Morton, C Oliver" uniqKey="Morton C" first="C Oliver" last="Morton">C Oliver Morton</name><affiliation wicri:level="1"><nlm:affiliation>University of Western Sydney, Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>University of Western Sydney, Sydney, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Barnes, Rosemary A" sort="Barnes, Rosemary A" uniqKey="Barnes R" first="Rosemary A" last="Barnes">Rosemary A. Barnes</name><affiliation wicri:level="1"><nlm:affiliation>Cardiff University, UHW, Cardiff, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Cardiff University, UHW, Cardiff</wicri:regionArea><wicri:noRegion>Cardiff</wicri:noRegion></affiliation></author><author><name sortKey="Donnelly, J Peter" sort="Donnelly, J Peter" uniqKey="Donnelly J" first="J Peter" last="Donnelly">J Peter Donnelly</name><affiliation wicri:level="3"><nlm:affiliation>Radboud University Medical Centre, Nijmegen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Radboud University Medical Centre, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="White, P Lewis" sort="White, P Lewis" uniqKey="White P" first="P Lewis" last="White">P Lewis White</name><affiliation wicri:level="1"><nlm:affiliation>Public Health Wales Microbiology Cardiff, Cardiff, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health Wales Microbiology Cardiff, Cardiff</wicri:regionArea><wicri:noRegion>Cardiff</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aspergillosis (diagnosis)</term><term>Aspergillosis (microbiology)</term><term>Aspergillus fumigatus (genetics)</term><term>Aspergillus fumigatus (isolation & purification)</term><term>DNA, Fungal (blood)</term><term>Humans</term><term>Models, Biological</term><term>Molecular Diagnostic Techniques (methods)</term><term>Plasma (microbiology)</term><term>Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term><term>Specimen Handling (methods)</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (sang)</term><term>Aspergillose (diagnostic)</term><term>Aspergillose (microbiologie)</term><term>Aspergillus fumigatus (génétique)</term><term>Aspergillus fumigatus (isolement et purification)</term><term>Facteurs temps</term><term>Humains</term><term>Modèles biologiques</term><term>Plasma sanguin (microbiologie)</term><term>Prélèvement biologique ()</term><term>Réaction de polymérisation en chaîne ()</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Aspergillosis</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Aspergillose</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Polymerase Chain Reaction</term><term>Specimen Handling</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Aspergillose</term><term>Plasma sanguin</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Aspergillosis</term><term>Plasma</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ADN fongique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Models, Biological</term><term>Sensitivity and Specificity</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term><term>Humains</term><term>Modèles biologiques</term><term>Prélèvement biologique</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The use of serum or plasma for Aspergillus PCR testing facilitates automated and standardized technology. Recommendations for serum testing are available, and while serum and plasma are regularly considered interchangeable for use in fungal diagnostics, differences in galactomannan enzyme immunoassay (GM-EIA) performance have been reported and are attributed to clot formation. Therefore, it is important to assess plasma PCR testing to determine if previous recommendations for serum are applicable and also to compare analytical performance with that of serum PCR. Molecular methods testing serum and plasma were compared through multicenter distribution of quality control panels, with additional studies to investigate the effect of clot formation and blood fractionation on DNA availability. Analytical sensitivity and time to positivity (TTP) were compared, and a regression analysis was performed to identify variables that enhanced plasma PCR performance. When testing plasma, sample volume, preextraction-to-postextraction volume ratio, PCR volume, duplicate testing, and the use of an internal control for PCR were positively associated with performance. When whole-blood samples were spiked and then fractionated, the analytical sensitivity and TTP were superior when testing plasma. Centrifugation had no effect on DNA availability, whereas the presence of clot material significantly lowered the concentration (P = 0.028). Technically, there are no major differences in the molecular processing of serum and plasma, but the formation of clot material potentially reduces available DNA in serum. During disease, Aspergillus DNA burdens in blood are often at the limits of PCR performance. Using plasma might improve performance while maintaining the methodological simplicity of serum testing.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26085614</PMID><DateCreated><Year>2015</Year><Month>08</Month><Day>19</Day></DateCreated><DateCompleted><Year>2016</Year><Month>05</Month><Day>09</Day></DateCompleted><DateRevised><Year>2015</Year><Month>09</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-660X</ISSN><JournalIssue CitedMedium="Internet"><Volume>53</Volume><Issue>9</Issue><PubDate><Year>2015</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Analytical Comparison of In Vitro-Spiked Human Serum and Plasma for PCR-Based Detection of Aspergillus fumigatus DNA: a Study by the European Aspergillus PCR Initiative.</ArticleTitle><Pagination><MedlinePgn>2838-45</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JCM.00906-15</ELocationID><Abstract><AbstractText>The use of serum or plasma for Aspergillus PCR testing facilitates automated and standardized technology. Recommendations for serum testing are available, and while serum and plasma are regularly considered interchangeable for use in fungal diagnostics, differences in galactomannan enzyme immunoassay (GM-EIA) performance have been reported and are attributed to clot formation. Therefore, it is important to assess plasma PCR testing to determine if previous recommendations for serum are applicable and also to compare analytical performance with that of serum PCR. Molecular methods testing serum and plasma were compared through multicenter distribution of quality control panels, with additional studies to investigate the effect of clot formation and blood fractionation on DNA availability. Analytical sensitivity and time to positivity (TTP) were compared, and a regression analysis was performed to identify variables that enhanced plasma PCR performance. When testing plasma, sample volume, preextraction-to-postextraction volume ratio, PCR volume, duplicate testing, and the use of an internal control for PCR were positively associated with performance. When whole-blood samples were spiked and then fractionated, the analytical sensitivity and TTP were superior when testing plasma. Centrifugation had no effect on DNA availability, whereas the presence of clot material significantly lowered the concentration (P = 0.028). Technically, there are no major differences in the molecular processing of serum and plasma, but the formation of clot material potentially reduces available DNA in serum. During disease, Aspergillus DNA burdens in blood are often at the limits of PCR performance. Using plasma might improve performance while maintaining the methodological simplicity of serum testing.</AbstractText><CopyrightInformation>Copyright © 2015 Loeffler et al.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Loeffler</LastName><ForeName>Juergen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Würzburg University, Würzburg, Germany loeffler_j@ukw.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mengoli</LastName><ForeName>Carlo</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>University of Padua, Padua, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Springer</LastName><ForeName>Jan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Würzburg University, Würzburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bretagne</LastName><ForeName>Stéphane</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Paris Diderot University, Saint Louis Hospital-APHP, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cuenca-Estrella</LastName><ForeName>Manuel</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Spanish National Centre for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Klingspor</LastName><ForeName>Lena</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Karolinska University Hospital, Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lagrou</LastName><ForeName>Katrien</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>National Reference Center for Mycosis, University Hospitals Leuven, Leuven, Belgium Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Melchers</LastName><ForeName>Willem J G</ForeName><Initials>WJ</Initials><AffiliationInfo><Affiliation>Radboud University Medical Centre, Nijmegen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morton</LastName><ForeName>C Oliver</ForeName><Initials>CO</Initials><AffiliationInfo><Affiliation>University of Western Sydney, Sydney, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barnes</LastName><ForeName>Rosemary A</ForeName><Initials>RA</Initials><AffiliationInfo><Affiliation>Cardiff University, UHW, Cardiff, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Donnelly</LastName><ForeName>J Peter</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Radboud University Medical Centre, Nijmegen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>White</LastName><ForeName>P Lewis</ForeName><Initials>PL</Initials><AffiliationInfo><Affiliation>Public Health Wales Microbiology Cardiff, Cardiff, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><CollectiveName>European Aspergillus PCR Initiative</CollectiveName></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016448">Multicenter Study</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>06</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2000 Oct;38(10):3830-3</RefSource><PMID Version="1">11015412</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2002 Apr;40(4):1567-8</RefSource><PMID Version="1">11923400</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Ann N Y Acad Sci. 1983 Jun 27;408:233-53</RefSource><PMID Version="1">6575687</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Invest. 1985 Dec;76(6):2182-90</RefSource><PMID Version="1">3001145</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Clin Infect Dis. 2006 Feb 15;42(4):479-86</RefSource><PMID Version="1">16421791</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Clin Infect Dis. 2008 Jun 15;46(12):1813-21</RefSource><PMID Version="1">18462102</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Clin Chem. 2009 Apr;55(4):611-22</RefSource><PMID Version="1">19246619</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Pathol. 2009 Jun;62(6):539-41</RefSource><PMID Version="1">19190008</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2010 Mar;48(3):811-6</RefSource><PMID Version="1">20042634</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2010 Apr;48(4):1231-40</RefSource><PMID Version="1">20147637</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2011 Jun;49(6):2169-74</RefSource><PMID Version="1">21450965</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2011 Nov;49(11):3842-8</RefSource><PMID Version="1">21940479</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Clin Vaccine Immunol. 2013 Apr;20(4):636-8</RefSource><PMID Version="1">23389933</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2013 May;51(5):1445-50</RefSource><PMID Version="1">23426930</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Thorax. 2015 Mar;70(3):270-7</RefSource><PMID Version="1">25354514</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D001228" MajorTopicYN="N">Aspergillosis</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001232" MajorTopicYN="N">Aspergillus fumigatus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008954" MajorTopicYN="N">Models, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025202" MajorTopicYN="N">Molecular Diagnostic Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010949" MajorTopicYN="N">Plasma</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013048" MajorTopicYN="N">Specimen Handling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4540929</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>04</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>06</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26085614</ArticleId><ArticleId IdType="pii">JCM.00906-15</ArticleId><ArticleId IdType="doi">10.1128/JCM.00906-15</ArticleId><ArticleId IdType="pmc">PMC4540929</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>Belgique</li><li>Espagne</li><li>France</li><li>Italie</li><li>Pays-Bas</li><li>Royaume-Uni</li><li>Suède</li></country><region><li>Bavière</li><li>Communauté de Madrid</li><li>District de Basse-Franconie</li><li>Gueldre</li><li>Svealand</li><li>Île-de-France</li></region><settlement><li>Madrid</li><li>Nimègue</li><li>Paris</li><li>Stockholm</li><li>Wurtzbourg</li></settlement></list><tree><country name="Allemagne"><region name="Bavière"><name sortKey="Loeffler, Juergen" sort="Loeffler, Juergen" uniqKey="Loeffler J" first="Juergen" last="Loeffler">Juergen Loeffler</name></region><name sortKey="Springer, Jan" sort="Springer, Jan" uniqKey="Springer J" first="Jan" last="Springer">Jan Springer</name></country><country name="Italie"><noRegion><name sortKey="Mengoli, Carlo" sort="Mengoli, Carlo" uniqKey="Mengoli C" first="Carlo" last="Mengoli">Carlo Mengoli</name></noRegion></country><country name="France"><region name="Île-de-France"><name sortKey="Bretagne, Stephane" sort="Bretagne, Stephane" uniqKey="Bretagne S" first="Stéphane" last="Bretagne">Stéphane Bretagne</name></region></country><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Cuenca Estrella, Manuel" sort="Cuenca Estrella, Manuel" uniqKey="Cuenca Estrella M" first="Manuel" last="Cuenca-Estrella">Manuel Cuenca-Estrella</name></region></country><country name="Suède"><region name="Svealand"><name sortKey="Klingspor, Lena" sort="Klingspor, Lena" uniqKey="Klingspor L" first="Lena" last="Klingspor">Lena Klingspor</name></region></country><country name="Belgique"><noRegion><name sortKey="Lagrou, Katrien" sort="Lagrou, Katrien" uniqKey="Lagrou K" first="Katrien" last="Lagrou">Katrien Lagrou</name></noRegion></country><country name="Pays-Bas"><region name="Gueldre"><name sortKey="Melchers, Willem J G" sort="Melchers, Willem J G" uniqKey="Melchers W" first="Willem J G" last="Melchers">Willem J G. Melchers</name></region><name sortKey="Donnelly, J Peter" sort="Donnelly, J Peter" uniqKey="Donnelly J" first="J Peter" last="Donnelly">J Peter Donnelly</name></country><country name="Australie"><noRegion><name sortKey="Morton, C Oliver" sort="Morton, C Oliver" uniqKey="Morton C" first="C Oliver" last="Morton">C Oliver Morton</name></noRegion></country><country name="Royaume-Uni"><noRegion><name sortKey="Barnes, Rosemary A" sort="Barnes, Rosemary A" uniqKey="Barnes R" first="Rosemary A" last="Barnes">Rosemary A. Barnes</name></noRegion><name sortKey="White, P Lewis" sort="White, P Lewis" uniqKey="White P" first="P Lewis" last="White">P Lewis White</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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