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Chronic erythropoietin treatment improves diet-induced glucose intolerance in rats.

Identifieur interne : 002E29 ( PubMed/Checkpoint ); précédent : 002E28; suivant : 002E30

Chronic erythropoietin treatment improves diet-induced glucose intolerance in rats.

Auteurs : Corinne Caillaud [Australie] ; Mie Mechta [Australie] ; Heidi Ainge [Australie] ; Andreas N. Madsen ; Patricia Ruell [Australie] ; Emilie Mas [Australie] ; Catherine Bisbal [Australie] ; Jacques Mercier ; Stephen Twigg [Australie] ; Trevor A. Mori [Australie] ; David Simar [Australie] ; Romain Barrès [Australie]

Source :

RBID : pubmed:25767056

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Abstract

Erythropoietin (EPO) ameliorates glucose metabolism through mechanisms not fully understood. In this study, we investigated the effect of EPO on glucose metabolism and insulin signaling in skeletal muscle. A 2-week EPO treatment of rats fed with a high-fat diet (HFD) improved fasting glucose levels and glucose tolerance, without altering total body weight or retroperitoneal fat mass. Concomitantly, EPO partially rescued insulin-stimulated AKT activation, reduced markers of oxidative stress, and restored heat-shock protein 72 expression in soleus muscles from HFD-fed rats. Incubation of skeletal muscle cell cultures with EPO failed to induce AKT phosphorylation and had no effect on glucose uptake or glycogen synthesis. We found that the EPO receptor gene was expressed in myotubes, but was undetectable in soleus. Together, our results indicate that EPO treatment improves glucose tolerance but does not directly activate the phosphorylation of AKT in muscle cells. We propose that the reduced systemic inflammation or oxidative stress that we observed after treatment with EPO could contribute to the improvement of whole-body glucose metabolism.

DOI: 10.1530/JOE-15-0010
PubMed: 25767056


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pubmed:25767056

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<title level="j">The Journal of endocrinology</title>
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<keywords scheme="KwdEn" xml:lang="en">
<term>Animals</term>
<term>Diet, High-Fat (adverse effects)</term>
<term>Erythropoietin (metabolism)</term>
<term>Glucose (metabolism)</term>
<term>Glucose Intolerance (enzymology)</term>
<term>Glucose Intolerance (genetics)</term>
<term>Glucose Intolerance (metabolism)</term>
<term>HSP72 Heat-Shock Proteins (genetics)</term>
<term>HSP72 Heat-Shock Proteins (metabolism)</term>
<term>Humans</term>
<term>Insulin (metabolism)</term>
<term>Male</term>
<term>Mice</term>
<term>Muscle, Skeletal (enzymology)</term>
<term>Muscle, Skeletal (metabolism)</term>
<term>Oxidative Stress</term>
<term>Proto-Oncogene Proteins c-akt (genetics)</term>
<term>Proto-Oncogene Proteins c-akt (metabolism)</term>
<term>Rats</term>
<term>Rats, Sprague-Dawley</term>
<term>Receptors, Erythropoietin (genetics)</term>
<term>Receptors, Erythropoietin (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>Alimentation riche en graisse (effets indésirables)</term>
<term>Animaux</term>
<term>Glucose (métabolisme)</term>
<term>Humains</term>
<term>Insuline (métabolisme)</term>
<term>Intolérance au glucose (enzymologie)</term>
<term>Intolérance au glucose (génétique)</term>
<term>Intolérance au glucose (métabolisme)</term>
<term>Muscles squelettiques (enzymologie)</term>
<term>Muscles squelettiques (métabolisme)</term>
<term>Mâle</term>
<term>Protéines du choc thermique HSP72 (génétique)</term>
<term>Protéines du choc thermique HSP72 (métabolisme)</term>
<term>Protéines proto-oncogènes c-akt (génétique)</term>
<term>Protéines proto-oncogènes c-akt (métabolisme)</term>
<term>Rat Sprague-Dawley</term>
<term>Rats</term>
<term>Récepteur érythropoïétine (génétique)</term>
<term>Récepteur érythropoïétine (métabolisme)</term>
<term>Souris</term>
<term>Stress oxydatif</term>
<term>Érythropoïétine (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>HSP72 Heat-Shock Proteins</term>
<term>Proto-Oncogene Proteins c-akt</term>
<term>Receptors, Erythropoietin</term>
</keywords>
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<term>Erythropoietin</term>
<term>Glucose</term>
<term>HSP72 Heat-Shock Proteins</term>
<term>Insulin</term>
<term>Proto-Oncogene Proteins c-akt</term>
<term>Receptors, Erythropoietin</term>
</keywords>
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<term>Diet, High-Fat</term>
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<term>Alimentation riche en graisse</term>
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<term>Intolérance au glucose</term>
<term>Muscles squelettiques</term>
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<term>Glucose Intolerance</term>
<term>Muscle, Skeletal</term>
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<term>Récepteur érythropoïétine</term>
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<term>Muscle, Skeletal</term>
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<term>Insuline</term>
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<term>Protéines proto-oncogènes c-akt</term>
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<term>Érythropoïétine</term>
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<term>Animals</term>
<term>Humans</term>
<term>Male</term>
<term>Mice</term>
<term>Oxidative Stress</term>
<term>Rats</term>
<term>Rats, Sprague-Dawley</term>
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<term>Animaux</term>
<term>Humains</term>
<term>Mâle</term>
<term>Rat Sprague-Dawley</term>
<term>Rats</term>
<term>Souris</term>
<term>Stress oxydatif</term>
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<div type="abstract" xml:lang="en">Erythropoietin (EPO) ameliorates glucose metabolism through mechanisms not fully understood. In this study, we investigated the effect of EPO on glucose metabolism and insulin signaling in skeletal muscle. A 2-week EPO treatment of rats fed with a high-fat diet (HFD) improved fasting glucose levels and glucose tolerance, without altering total body weight or retroperitoneal fat mass. Concomitantly, EPO partially rescued insulin-stimulated AKT activation, reduced markers of oxidative stress, and restored heat-shock protein 72 expression in soleus muscles from HFD-fed rats. Incubation of skeletal muscle cell cultures with EPO failed to induce AKT phosphorylation and had no effect on glucose uptake or glycogen synthesis. We found that the EPO receptor gene was expressed in myotubes, but was undetectable in soleus. Together, our results indicate that EPO treatment improves glucose tolerance but does not directly activate the phosphorylation of AKT in muscle cells. We propose that the reduced systemic inflammation or oxidative stress that we observed after treatment with EPO could contribute to the improvement of whole-body glucose metabolism.</div>
</front>
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<DateCreated>
<Year>2015</Year>
<Month>05</Month>
<Day>09</Day>
</DateCreated>
<DateCompleted>
<Year>2015</Year>
<Month>08</Month>
<Day>10</Day>
</DateCompleted>
<DateRevised>
<Year>2015</Year>
<Month>05</Month>
<Day>09</Day>
</DateRevised>
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<Journal>
<ISSN IssnType="Electronic">1479-6805</ISSN>
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<Volume>225</Volume>
<Issue>2</Issue>
<PubDate>
<Year>2015</Year>
<Month>May</Month>
</PubDate>
</JournalIssue>
<Title>The Journal of endocrinology</Title>
<ISOAbbreviation>J. Endocrinol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Chronic erythropoietin treatment improves diet-induced glucose intolerance in rats.</ArticleTitle>
<Pagination>
<MedlinePgn>77-88</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1530/JOE-15-0010</ELocationID>
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<AbstractText>Erythropoietin (EPO) ameliorates glucose metabolism through mechanisms not fully understood. In this study, we investigated the effect of EPO on glucose metabolism and insulin signaling in skeletal muscle. A 2-week EPO treatment of rats fed with a high-fat diet (HFD) improved fasting glucose levels and glucose tolerance, without altering total body weight or retroperitoneal fat mass. Concomitantly, EPO partially rescued insulin-stimulated AKT activation, reduced markers of oxidative stress, and restored heat-shock protein 72 expression in soleus muscles from HFD-fed rats. Incubation of skeletal muscle cell cultures with EPO failed to induce AKT phosphorylation and had no effect on glucose uptake or glycogen synthesis. We found that the EPO receptor gene was expressed in myotubes, but was undetectable in soleus. Together, our results indicate that EPO treatment improves glucose tolerance but does not directly activate the phosphorylation of AKT in muscle cells. We propose that the reduced systemic inflammation or oxidative stress that we observed after treatment with EPO could contribute to the improvement of whole-body glucose metabolism.</AbstractText>
<CopyrightInformation>© 2015 The authors.</CopyrightInformation>
</Abstract>
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<LastName>Caillaud</LastName>
<ForeName>Corinne</ForeName>
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<Affiliation>Exercise Health and Performance Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Faculty of Health and Medical Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark Department of Neuroscience and Pharmacology Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark School of Medicine and Pharmacology Royal Perth Hospital, The University of Western Australia, Perth, Western Australia, Australia UMR CNRS 9214 U1046 INSERM Physiologie et Médecine Expérimentale du Cœur et des Muscles, Université de Montpellier, Montpellier, France Physiology Department CHU Arnaud de Villeneuve, Montpellier, France Department of Endocrinology Sydney Medical School, Royal Prince Alfred Hospital, University of Sydney, Camperdown, New South Wales, Australia Inflammation and Infection Research School of Medical Sciences, UNSW Australia, Sydney, New South Wales, Australia corinne.caillaud@sydney.edu.au barres@sund.ku.dk.</Affiliation>
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<LastName>Mechta</LastName>
<ForeName>Mie</ForeName>
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<Affiliation>Exercise Health and Performance Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Faculty of Health and Medical Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark Department of Neuroscience and Pharmacology Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark School of Medicine and Pharmacology Royal Perth Hospital, The University of Western Australia, Perth, Western Australia, Australia UMR CNRS 9214 U1046 INSERM Physiologie et Médecine Expérimentale du Cœur et des Muscles, Université de Montpellier, Montpellier, France Physiology Department CHU Arnaud de Villeneuve, Montpellier, France Department of Endocrinology Sydney Medical School, Royal Prince Alfred Hospital, University of Sydney, Camperdown, New South Wales, Australia Inflammation and Infection Research School of Medical Sciences, UNSW Australia, Sydney, New South Wales, Australia.</Affiliation>
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<Affiliation>Exercise Health and Performance Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Faculty of Health and Medical Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark Department of Neuroscience and Pharmacology Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark School of Medicine and Pharmacology Royal Perth Hospital, The University of Western Australia, Perth, Western Australia, Australia UMR CNRS 9214 U1046 INSERM Physiologie et Médecine Expérimentale du Cœur et des Muscles, Université de Montpellier, Montpellier, France Physiology Department CHU Arnaud de Villeneuve, Montpellier, France Department of Endocrinology Sydney Medical School, Royal Prince Alfred Hospital, University of Sydney, Camperdown, New South Wales, Australia Inflammation and Infection Research School of Medical Sciences, UNSW Australia, Sydney, New South Wales, Australia Exercise Health and Performance Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Faculty of Health and Medical Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark Department of Neuroscience and Pharmacology Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark School of Medicine and Pharmacology Royal Perth Hospital, The University of Western Australia, Perth, Western Australia, Australia UMR CNRS 9214 U1046 INSERM Physiologie et Médecine Expérimentale du Cœur et des Muscles, Université de Montpellier, Montpellier, France Physiology Department CHU Arnaud de Villeneuve, Montpellier, France Department of Endocrinology Sydney Medical School, Royal Prince Alfred Hospital, University of Sydney, Camperdown, New South Wales, Australia Inflammation and Infection Research School of Medical Sciences, UNSW Australia, Sydney, N</Affiliation>
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<Affiliation>Exercise Health and Performance Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Faculty of Health and Medical Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark Department of Neuroscience and Pharmacology Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark School of Medicine and Pharmacology Royal Perth Hospital, The University of Western Australia, Perth, Western Australia, Australia UMR CNRS 9214 U1046 INSERM Physiologie et Médecine Expérimentale du Cœur et des Muscles, Université de Montpellier, Montpellier, France Physiology Department CHU Arnaud de Villeneuve, Montpellier, France Department of Endocrinology Sydney Medical School, Royal Prince Alfred Hospital, University of Sydney, Camperdown, New South Wales, Australia Inflammation and Infection Research School of Medical Sciences, UNSW Australia, Sydney, New South Wales, Australia.</Affiliation>
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<Affiliation>Exercise Health and Performance Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Faculty of Health and Medical Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark Department of Neuroscience and Pharmacology Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark School of Medicine and Pharmacology Royal Perth Hospital, The University of Western Australia, Perth, Western Australia, Australia UMR CNRS 9214 U1046 INSERM Physiologie et Médecine Expérimentale du Cœur et des Muscles, Université de Montpellier, Montpellier, France Physiology Department CHU Arnaud de Villeneuve, Montpellier, France Department of Endocrinology Sydney Medical School, Royal Prince Alfred Hospital, University of Sydney, Camperdown, New South Wales, Australia Inflammation and Infection Research School of Medical Sciences, UNSW Australia, Sydney, New South Wales, Australia.</Affiliation>
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<Affiliation>Exercise Health and Performance Faculty of Health Sciences, and Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Faculty of Health and Medical Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark Department of Neuroscience and Pharmacology Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark School of Medicine and Pharmacology Royal Perth Hospital, The University of Western Australia, Perth, Western Australia, Australia UMR CNRS 9214 U1046 INSERM Physiologie et Médecine Expérimentale du Cœur et des Muscles, Université de Montpellier, Montpellier, France Physiology Department CHU Arnaud de Villeneuve, Montpellier, France Department of Endocrinology Sydney Medical School, Royal Prince Alfred Hospital, University of Sydney, Camperdown, New South Wales, Australia Inflammation and Infection Research School of Medical Sciences, UNSW Australia, Sydney, New South Wales, Australia corinne.caillaud@sydney.edu.au barres@sund.ku.dk.</Affiliation>
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<name sortKey="Barres, Romain" sort="Barres, Romain" uniqKey="Barres R" first="Romain" last="Barrès">Romain Barrès</name>
<name sortKey="Bisbal, Catherine" sort="Bisbal, Catherine" uniqKey="Bisbal C" first="Catherine" last="Bisbal">Catherine Bisbal</name>
<name sortKey="Mas, Emilie" sort="Mas, Emilie" uniqKey="Mas E" first="Emilie" last="Mas">Emilie Mas</name>
<name sortKey="Mechta, Mie" sort="Mechta, Mie" uniqKey="Mechta M" first="Mie" last="Mechta">Mie Mechta</name>
<name sortKey="Mori, Trevor A" sort="Mori, Trevor A" uniqKey="Mori T" first="Trevor A" last="Mori">Trevor A. Mori</name>
<name sortKey="Ruell, Patricia" sort="Ruell, Patricia" uniqKey="Ruell P" first="Patricia" last="Ruell">Patricia Ruell</name>
<name sortKey="Simar, David" sort="Simar, David" uniqKey="Simar D" first="David" last="Simar">David Simar</name>
<name sortKey="Twigg, Stephen" sort="Twigg, Stephen" uniqKey="Twigg S" first="Stephen" last="Twigg">Stephen Twigg</name>
</country>
</tree>
</affiliations>
</record>

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